Dialister micraerophilus DSM 19965

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Dialister; Dialister micraerophilus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2BY57|F2BY57_9FIRM Serine-type D-Ala-D-Ala carboxypeptidase OS=Dialister micraerophilus DSM 19965 OX=888062 GN=dacB PE=3 SV=1
MM1 pKa = 8.3DD2 pKa = 5.65DD3 pKa = 3.5MQVYY7 pKa = 9.61IANLGKK13 pKa = 10.45YY14 pKa = 9.93NEE16 pKa = 4.62GEE18 pKa = 4.02LVGAWFTFPIDD29 pKa = 3.62FEE31 pKa = 4.44EE32 pKa = 4.38VKK34 pKa = 10.79EE35 pKa = 4.14KK36 pKa = 10.6IGLNDD41 pKa = 3.31EE42 pKa = 4.29YY43 pKa = 11.02EE44 pKa = 4.27EE45 pKa = 4.26YY46 pKa = 10.57AIHH49 pKa = 7.92DD50 pKa = 4.17YY51 pKa = 10.78EE52 pKa = 5.43LPFTVDD58 pKa = 3.5EE59 pKa = 4.3YY60 pKa = 11.25TSIGEE65 pKa = 4.27LNRR68 pKa = 11.84LWEE71 pKa = 4.42MVSEE75 pKa = 4.35LPEE78 pKa = 4.12EE79 pKa = 4.32LQSEE83 pKa = 4.63LSALLTHH90 pKa = 6.76FSSIEE95 pKa = 3.84EE96 pKa = 3.84LSEE99 pKa = 3.9YY100 pKa = 10.62QEE102 pKa = 5.43DD103 pKa = 4.22IIIHH107 pKa = 5.74SDD109 pKa = 3.6CDD111 pKa = 3.75DD112 pKa = 3.61MADD115 pKa = 3.27VARR118 pKa = 11.84YY119 pKa = 9.18YY120 pKa = 10.14IEE122 pKa = 3.93EE123 pKa = 4.23TGVLGEE129 pKa = 4.82VPTSLQNYY137 pKa = 8.58IDD139 pKa = 3.88YY140 pKa = 10.71EE141 pKa = 4.63SYY143 pKa = 11.25GRR145 pKa = 11.84DD146 pKa = 3.37LEE148 pKa = 4.23ISGTFITTNYY158 pKa = 10.31GIFEE162 pKa = 4.06IVYY165 pKa = 10.0

Molecular weight: 19.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2BWM8|F2BWM8_9FIRM Adenylosuccinate synthetase OS=Dialister micraerophilus DSM 19965 OX=888062 GN=purA PE=3 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNHH11 pKa = 5.38WRR13 pKa = 11.84KK14 pKa = 7.63KK15 pKa = 6.32THH17 pKa = 5.67GFRR20 pKa = 11.84ARR22 pKa = 11.84MKK24 pKa = 8.69TRR26 pKa = 11.84AGRR29 pKa = 11.84IVLKK33 pKa = 10.39RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.89GRR40 pKa = 11.84KK41 pKa = 8.67VLSAA45 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1242

0

1242

376269

37

1694

303.0

34.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.705 ± 0.085

1.172 ± 0.025

5.104 ± 0.055

7.321 ± 0.087

4.376 ± 0.054

6.794 ± 0.077

1.744 ± 0.03

8.871 ± 0.067

8.813 ± 0.081

8.797 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.878 ± 0.032

5.226 ± 0.068

3.108 ± 0.038

2.674 ± 0.034

3.931 ± 0.062

6.044 ± 0.054

5.165 ± 0.049

6.701 ± 0.072

0.815 ± 0.021

3.761 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski