Rhodobacter sp. CACIA14H1
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3570 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V7EJG3|V7EJG3_9RHOB Uncharacterized protein OS=Rhodobacter sp. CACIA14H1 OX=1408890 GN=Q27BPR15_13750 PE=4 SV=1
MM1 pKa = 7.77 LFLLLAAFGLPANALNLSGASVEE24 pKa = 5.16 LIYY27 pKa = 10.86 SDD29 pKa = 3.87 FGSYY33 pKa = 10.32 GSAPVGSGVEE43 pKa = 3.87 FDD45 pKa = 4.15 EE46 pKa = 5.49 GFGALTYY53 pKa = 10.88 DD54 pKa = 3.71 VDD56 pKa = 4.0 PANNTVTLHH65 pKa = 6.74 LSNSNPWGDD74 pKa = 3.45 TTATLRR80 pKa = 11.84 FSGGSVTDD88 pKa = 3.96 FTSVAVTSNTTGQTYY103 pKa = 7.37 TASVSGKK110 pKa = 10.02 DD111 pKa = 3.56 LVITLPVNSGAGDD124 pKa = 3.58 VMFTLSDD131 pKa = 3.66 TPLDD135 pKa = 3.91 TVQPTVTGLTASSGRR150 pKa = 11.84 YY151 pKa = 9.59 GIGQTVQVSVSFSEE165 pKa = 4.38 VVYY168 pKa = 9.36 VTGTPQLTLEE178 pKa = 4.32 TGATDD183 pKa = 3.89 RR184 pKa = 11.84 VASYY188 pKa = 11.11 LSGSGTSTLVFSYY201 pKa = 9.36 TVQAGDD207 pKa = 3.25 TAADD211 pKa = 3.8 LDD213 pKa = 4.25 YY214 pKa = 11.61 VAINSLALNGGTIRR228 pKa = 11.84 DD229 pKa = 3.65 AASNNAVLVLPSPGAANSLGANANIIVDD257 pKa = 3.96 GVVPTVSSVTSPTSNGTYY275 pKa = 8.7 KK276 pKa = 10.53 TGDD279 pKa = 3.7 TISVVVNFSEE289 pKa = 4.57 TVIVTGTPQLTLEE302 pKa = 4.44 TGLTDD307 pKa = 4.15 RR308 pKa = 11.84 SASYY312 pKa = 11.11 SSGSGTSALTFTYY325 pKa = 9.54 TVQSGDD331 pKa = 3.11 TTADD335 pKa = 3.34 LDD337 pKa = 4.26 YY338 pKa = 11.45 VATNSLALNGGSIRR352 pKa = 11.84 DD353 pKa = 3.57 EE354 pKa = 4.51 AGNNATLTLASPGAANSLGANRR376 pKa = 11.84 NIVIDD381 pKa = 5.08 AISPTVTSVTSTTANDD397 pKa = 3.74 TYY399 pKa = 11.18 KK400 pKa = 10.41 IGDD403 pKa = 3.91 VISISVNFSEE413 pKa = 4.7 TVTVTGTPQLTLEE426 pKa = 4.32 TGATDD431 pKa = 3.52 RR432 pKa = 11.84 VVNYY436 pKa = 10.43 ASGSGTGTLVFSYY449 pKa = 9.2 TVQAGDD455 pKa = 3.22 QSADD459 pKa = 3.24 LDD461 pKa = 4.35 YY462 pKa = 11.58 ASTAALALNGGTIRR476 pKa = 11.84 DD477 pKa = 3.74 AAGNAATLTLASPGSANSLGANKK500 pKa = 10.27 NIVIDD505 pKa = 4.11 GVRR508 pKa = 11.84 PAVTSIAPAGSTFGGASSVTFSVTFSEE535 pKa = 4.97 TVTGVTPDD543 pKa = 3.99 DD544 pKa = 3.79 FALTVTGTAAGTIVSVSHH562 pKa = 6.69 SGTTSDD568 pKa = 3.54 VTVGSISGDD577 pKa = 3.02 GTLRR581 pKa = 11.84 LDD583 pKa = 3.99 LRR585 pKa = 11.84 GSTDD589 pKa = 2.41 ICC591 pKa = 4.89
Molecular weight: 59.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.719
IPC_protein 3.757
Toseland 3.516
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.427
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.177
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.884
Patrickios 1.252
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|V7EI79|V7EI79_9RHOB DNA polymerase III subunit epsilon (Fragment) OS=Rhodobacter sp. CACIA14H1 OX=1408890 GN=Q27BPR15_14575 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.34 GGRR28 pKa = 11.84 LVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.92 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3570
0
3570
1060311
29
3616
297.0
32.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.528 ± 0.065
0.871 ± 0.016
5.805 ± 0.052
5.464 ± 0.037
3.596 ± 0.028
9.212 ± 0.071
2.042 ± 0.022
4.68 ± 0.031
2.77 ± 0.037
10.292 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.735 ± 0.023
2.25 ± 0.024
5.379 ± 0.034
2.886 ± 0.022
7.228 ± 0.042
4.602 ± 0.024
5.485 ± 0.038
7.666 ± 0.041
1.473 ± 0.023
2.035 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here