Amino acid dipepetide frequency for Megasphaera cerevisiae DSM 20462

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.968AlaAla: 10.968 ± 0.145	1.354AlaCys: 1.354 ± 0.044	5.196AlaAsp: 5.196 ± 0.095	5.136AlaGlu: 5.136 ± 0.077	3.387AlaPhe: 3.387 ± 0.056	7.512AlaGly: 7.512 ± 0.117	1.726AlaHis: 1.726 ± 0.04	5.934AlaIle: 5.934 ± 0.094	4.375AlaLys: 4.375 ± 0.079	7.983AlaLeu: 7.983 ± 0.107	2.808AlaMet: 2.808 ± 0.058	2.608AlaAsn: 2.608 ± 0.06	2.561AlaPro: 2.561 ± 0.057	2.997AlaGln: 2.997 ± 0.068	3.49AlaArg: 3.49 ± 0.072	4.453AlaSer: 4.453 ± 0.076	3.458AlaThr: 3.458 ± 0.071	8.232AlaVal: 8.232 ± 0.116	0.827AlaTrp: 0.827 ± 0.032	3.114AlaTyr: 3.114 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	1.1CysAla: 1.1 ± 0.041	0.298CysCys: 0.298 ± 0.018	0.753CysAsp: 0.753 ± 0.031	0.675CysGlu: 0.675 ± 0.032	0.539CysPhe: 0.539 ± 0.026	1.437CysGly: 1.437 ± 0.044	0.421CysHis: 0.421 ± 0.022	1.145CysIle: 1.145 ± 0.038	0.618CysLys: 0.618 ± 0.027	1.24CysLeu: 1.24 ± 0.037	0.439CysMet: 0.439 ± 0.023	0.502CysAsn: 0.502 ± 0.025	0.747CysPro: 0.747 ± 0.033	0.499CysGln: 0.499 ± 0.023	1.024CysArg: 1.024 ± 0.039	0.863CysSer: 0.863 ± 0.035	0.838CysThr: 0.838 ± 0.036	0.933CysVal: 0.933 ± 0.036	0.119CysTrp: 0.119 ± 0.011	0.443CysTyr: 0.443 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	4.692AspAla: 4.692 ± 0.093	0.767AspCys: 0.767 ± 0.032	2.716AspAsp: 2.716 ± 0.057	3.453AspGlu: 3.453 ± 0.073	2.289AspPhe: 2.289 ± 0.056	4.151AspGly: 4.151 ± 0.085	1.189AspHis: 1.189 ± 0.036	4.857AspIle: 4.857 ± 0.077	3.187AspLys: 3.187 ± 0.068	4.315AspLeu: 4.315 ± 0.076	2.011AspMet: 2.011 ± 0.052	2.006AspAsn: 2.006 ± 0.053	2.025AspPro: 2.025 ± 0.054	1.558AspGln: 1.558 ± 0.043	2.504AspArg: 2.504 ± 0.056	2.811AspSer: 2.811 ± 0.059	3.503AspThr: 3.503 ± 0.07	4.179AspVal: 4.179 ± 0.066	0.613AspTrp: 0.613 ± 0.029	2.203AspTyr: 2.203 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	5.217GluAla: 5.217 ± 0.081	0.674GluCys: 0.674 ± 0.029	3.06GluAsp: 3.06 ± 0.063	4.352GluGlu: 4.352 ± 0.083	2.025GluPhe: 2.025 ± 0.055	3.713GluGly: 3.713 ± 0.06	1.285GluHis: 1.285 ± 0.035	4.288GluIle: 4.288 ± 0.074	4.826GluLys: 4.826 ± 0.078	5.549GluLeu: 5.549 ± 0.087	1.89GluMet: 1.89 ± 0.054	2.734GluAsn: 2.734 ± 0.061	1.785GluPro: 1.785 ± 0.041	2.631GluGln: 2.631 ± 0.058	3.023GluArg: 3.023 ± 0.075	2.672GluSer: 2.672 ± 0.061	3.23GluThr: 3.23 ± 0.063	3.545GluVal: 3.545 ± 0.07	0.605GluTrp: 0.605 ± 0.028	2.163GluTyr: 2.163 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	3.148PheAla: 3.148 ± 0.061	0.665PheCys: 0.665 ± 0.026	2.359PheAsp: 2.359 ± 0.048	1.839PheGlu: 1.839 ± 0.048	1.92PhePhe: 1.92 ± 0.06	3.142PheGly: 3.142 ± 0.07	0.92PheHis: 0.92 ± 0.033	3.051PheIle: 3.051 ± 0.061	1.768PheLys: 1.768 ± 0.042	3.639PheLeu: 3.639 ± 0.073	1.193PheMet: 1.193 ± 0.038	1.467PheAsn: 1.467 ± 0.046	1.504PhePro: 1.504 ± 0.041	1.218PheGln: 1.218 ± 0.032	1.693PheArg: 1.693 ± 0.044	2.859PheSer: 2.859 ± 0.068	2.505PheThr: 2.505 ± 0.053	2.658PheVal: 2.658 ± 0.06	0.429PheTrp: 0.429 ± 0.022	1.453PheTyr: 1.453 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	6.111GlyAla: 6.111 ± 0.105	1.317GlyCys: 1.317 ± 0.043	3.604GlyAsp: 3.604 ± 0.069	3.63GlyGlu: 3.63 ± 0.071	3.045GlyPhe: 3.045 ± 0.06	5.589GlyGly: 5.589 ± 0.112	1.717GlyHis: 1.717 ± 0.044	6.464GlyIle: 6.464 ± 0.095	4.84GlyLys: 4.84 ± 0.083	6.456GlyLeu: 6.456 ± 0.104	2.64GlyMet: 2.64 ± 0.046	3.011GlyAsn: 3.011 ± 0.076	1.899GlyPro: 1.899 ± 0.042	2.593GlyGln: 2.593 ± 0.065	3.688GlyArg: 3.688 ± 0.068	4.482GlySer: 4.482 ± 0.095	5.027GlyThr: 5.027 ± 0.116	5.098GlyVal: 5.098 ± 0.083	0.781GlyTrp: 0.781 ± 0.033	3.118GlyTyr: 3.118 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.693HisAla: 1.693 ± 0.042	0.421HisCys: 0.421 ± 0.019	1.29HisAsp: 1.29 ± 0.037	1.237HisGlu: 1.237 ± 0.035	0.943HisPhe: 0.943 ± 0.032	1.721HisGly: 1.721 ± 0.05	0.696HisHis: 0.696 ± 0.035	2.062HisIle: 2.062 ± 0.052	1.051HisLys: 1.051 ± 0.034	1.988HisLeu: 1.988 ± 0.048	0.752HisMet: 0.752 ± 0.03	0.881HisAsn: 0.881 ± 0.036	1.131HisPro: 1.131 ± 0.037	0.782HisGln: 0.782 ± 0.03	1.017HisArg: 1.017 ± 0.036	1.189HisSer: 1.189 ± 0.037	1.424HisThr: 1.424 ± 0.043	1.651HisVal: 1.651 ± 0.043	0.253HisTrp: 0.253 ± 0.016	0.773HisTyr: 0.773 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.697IleAla: 6.697 ± 0.108	1.281IleCys: 1.281 ± 0.039	4.201IleAsp: 4.201 ± 0.08	4.0IleGlu: 4.0 ± 0.06	2.888IlePhe: 2.888 ± 0.068	5.663IleGly: 5.663 ± 0.083	1.802IleHis: 1.802 ± 0.042	5.528IleIle: 5.528 ± 0.094	3.599IleLys: 3.599 ± 0.081	6.807IleLeu: 6.807 ± 0.096	2.184IleMet: 2.184 ± 0.052	2.759IleAsn: 2.759 ± 0.055	3.435IlePro: 3.435 ± 0.062	2.558IleGln: 2.558 ± 0.053	3.719IleArg: 3.719 ± 0.067	4.716IleSer: 4.716 ± 0.075	4.4IleThr: 4.4 ± 0.078	5.33IleVal: 5.33 ± 0.083	0.622IleTrp: 0.622 ± 0.026	2.514IleTyr: 2.514 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	4.78LysAla: 4.78 ± 0.085	0.527LysCys: 0.527 ± 0.029	3.187LysAsp: 3.187 ± 0.066	4.448LysGlu: 4.448 ± 0.09	1.624LysPhe: 1.624 ± 0.051	3.781LysGly: 3.781 ± 0.069	1.176LysHis: 1.176 ± 0.037	4.308LysIle: 4.308 ± 0.078	4.969LysLys: 4.969 ± 0.095	4.347LysLeu: 4.347 ± 0.074	1.921LysMet: 1.921 ± 0.046	2.989LysAsn: 2.989 ± 0.068	1.926LysPro: 1.926 ± 0.053	2.377LysGln: 2.377 ± 0.06	2.82LysArg: 2.82 ± 0.057	2.826LysSer: 2.826 ± 0.055	3.413LysThr: 3.413 ± 0.065	3.458LysVal: 3.458 ± 0.076	0.584LysTrp: 0.584 ± 0.026	2.172LysTyr: 2.172 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	8.062LeuAla: 8.062 ± 0.111	1.436LeuCys: 1.436 ± 0.042	4.718LeuAsp: 4.718 ± 0.082	4.942LeuGlu: 4.942 ± 0.083	3.623LeuPhe: 3.623 ± 0.066	6.633LeuGly: 6.633 ± 0.088	2.14LeuHis: 2.14 ± 0.056	5.905LeuIle: 5.905 ± 0.107	5.002LeuLys: 5.002 ± 0.077	8.291LeuLeu: 8.291 ± 0.118	2.419LeuMet: 2.419 ± 0.056	3.266LeuAsn: 3.266 ± 0.061	4.091LeuPro: 4.091 ± 0.063	3.905LeuGln: 3.905 ± 0.074	4.497LeuArg: 4.497 ± 0.08	5.527LeuSer: 5.527 ± 0.082	5.483LeuThr: 5.483 ± 0.091	5.454LeuVal: 5.454 ± 0.082	0.804LeuTrp: 0.804 ± 0.033	3.044LeuTyr: 3.044 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.97MetAla: 2.97 ± 0.068	0.288MetCys: 0.288 ± 0.019	1.882MetAsp: 1.882 ± 0.054	2.068MetGlu: 2.068 ± 0.051	0.874MetPhe: 0.874 ± 0.028	2.299MetGly: 2.299 ± 0.056	0.622MetHis: 0.622 ± 0.028	2.15MetIle: 2.15 ± 0.061	2.25MetLys: 2.25 ± 0.045	2.666MetLeu: 2.666 ± 0.056	0.91MetMet: 0.91 ± 0.037	1.534MetAsn: 1.534 ± 0.043	1.373MetPro: 1.373 ± 0.044	1.13MetGln: 1.13 ± 0.033	1.42MetArg: 1.42 ± 0.047	1.653MetSer: 1.653 ± 0.047	2.069MetThr: 2.069 ± 0.043	1.86MetVal: 1.86 ± 0.044	0.193MetTrp: 0.193 ± 0.014	0.965MetTyr: 0.965 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.117AsnAla: 3.117 ± 0.07	0.535AsnCys: 0.535 ± 0.028	1.95AsnAsp: 1.95 ± 0.055	2.152AsnGlu: 2.152 ± 0.053	1.436AsnPhe: 1.436 ± 0.044	3.07AsnGly: 3.07 ± 0.082	0.908AsnHis: 0.908 ± 0.036	3.163AsnIle: 3.163 ± 0.056	2.11AsnLys: 2.11 ± 0.054	3.295AsnLeu: 3.295 ± 0.057	1.33AsnMet: 1.33 ± 0.039	1.563AsnAsn: 1.563 ± 0.05	1.929AsnPro: 1.929 ± 0.049	1.287AsnGln: 1.287 ± 0.044	1.809AsnArg: 1.809 ± 0.046	1.982AsnSer: 1.982 ± 0.062	2.441AsnThr: 2.441 ± 0.061	2.761AsnVal: 2.761 ± 0.063	0.399AsnTrp: 0.399 ± 0.019	1.453AsnTyr: 1.453 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.413ProAla: 3.413 ± 0.068	0.479ProCys: 0.479 ± 0.024	2.624ProAsp: 2.624 ± 0.055	2.853ProGlu: 2.853 ± 0.055	1.704ProPhe: 1.704 ± 0.049	2.651ProGly: 2.651 ± 0.052	0.916ProHis: 0.916 ± 0.026	2.411ProIle: 2.411 ± 0.049	1.791ProLys: 1.791 ± 0.045	3.392ProLeu: 3.392 ± 0.065	1.084ProMet: 1.084 ± 0.039	1.307ProAsn: 1.307 ± 0.036	1.067ProPro: 1.067 ± 0.034	1.55ProGln: 1.55 ± 0.039	1.379ProArg: 1.379 ± 0.043	2.073ProSer: 2.073 ± 0.047	1.817ProThr: 1.817 ± 0.051	3.462ProVal: 3.462 ± 0.066	0.39ProTrp: 0.39 ± 0.021	1.59ProTyr: 1.59 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	3.581GlnAla: 3.581 ± 0.06	0.521GlnCys: 0.521 ± 0.026	1.787GlnAsp: 1.787 ± 0.052	2.543GlnGlu: 2.543 ± 0.061	1.349GlnPhe: 1.349 ± 0.042	2.483GlnGly: 2.483 ± 0.06	0.911GlnHis: 0.911 ± 0.035	2.696GlnIle: 2.696 ± 0.052	2.557GlnLys: 2.557 ± 0.057	3.231GlnLeu: 3.231 ± 0.075	1.135GlnMet: 1.135 ± 0.036	1.542GlnAsn: 1.542 ± 0.05	1.262GlnPro: 1.262 ± 0.039	1.838GlnGln: 1.838 ± 0.057	1.921GlnArg: 1.921 ± 0.051	1.965GlnSer: 1.965 ± 0.053	1.978GlnThr: 1.978 ± 0.051	2.294GlnVal: 2.294 ± 0.055	0.414GlnTrp: 0.414 ± 0.025	1.659GlnTyr: 1.659 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	3.365ArgAla: 3.365 ± 0.058	0.681ArgCys: 0.681 ± 0.03	2.537ArgAsp: 2.537 ± 0.053	3.173ArgGlu: 3.173 ± 0.065	1.871ArgPhe: 1.871 ± 0.044	2.704ArgGly: 2.704 ± 0.057	1.27ArgHis: 1.27 ± 0.039	3.803ArgIle: 3.803 ± 0.068	2.988ArgLys: 2.988 ± 0.078	4.404ArgLeu: 4.404 ± 0.08	1.543ArgMet: 1.543 ± 0.044	1.981ArgAsn: 1.981 ± 0.05	1.784ArgPro: 1.784 ± 0.046	2.423ArgGln: 2.423 ± 0.07	3.024ArgArg: 3.024 ± 0.067	2.408ArgSer: 2.408 ± 0.05	2.457ArgThr: 2.457 ± 0.054	2.92ArgVal: 2.92 ± 0.066	0.52ArgTrp: 0.52 ± 0.027	1.855ArgTyr: 1.855 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.471SerAla: 4.471 ± 0.078	0.795SerCys: 0.795 ± 0.037	3.032SerAsp: 3.032 ± 0.075	2.919SerGlu: 2.919 ± 0.06	2.562SerPhe: 2.562 ± 0.055	4.869SerGly: 4.869 ± 0.094	1.334SerHis: 1.334 ± 0.038	3.887SerIle: 3.887 ± 0.074	2.666SerLys: 2.666 ± 0.057	5.288SerLeu: 5.288 ± 0.082	1.715SerMet: 1.715 ± 0.049	1.978SerAsn: 1.978 ± 0.05	2.065SerPro: 2.065 ± 0.046	2.143SerGln: 2.143 ± 0.05	2.78SerArg: 2.78 ± 0.06	3.199SerSer: 3.199 ± 0.084	2.792SerThr: 2.792 ± 0.068	4.28SerVal: 4.28 ± 0.089	0.65SerTrp: 0.65 ± 0.03	2.186SerTyr: 2.186 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	5.692ThrAla: 5.692 ± 0.097	0.738ThrCys: 0.738 ± 0.029	3.33ThrAsp: 3.33 ± 0.072	3.329ThrGlu: 3.329 ± 0.07	2.184ThrPhe: 2.184 ± 0.057	5.045ThrGly: 5.045 ± 0.082	1.192ThrHis: 1.192 ± 0.036	4.292ThrIle: 4.292 ± 0.072	2.716ThrLys: 2.716 ± 0.055	5.221ThrLeu: 5.221 ± 0.093	1.591ThrMet: 1.591 ± 0.046	1.928ThrAsn: 1.928 ± 0.063	2.519ThrPro: 2.519 ± 0.054	1.665ThrGln: 1.665 ± 0.044	2.154ThrArg: 2.154 ± 0.053	2.827ThrSer: 2.827 ± 0.061	3.023ThrThr: 3.023 ± 0.07	4.827ThrVal: 4.827 ± 0.106	0.584ThrTrp: 0.584 ± 0.026	2.205ThrTyr: 2.205 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	5.361ValAla: 5.361 ± 0.082	1.137ValCys: 1.137 ± 0.038	3.84ValAsp: 3.84 ± 0.076	3.785ValGlu: 3.785 ± 0.079	3.096ValPhe: 3.096 ± 0.065	4.882ValGly: 4.882 ± 0.094	1.474ValHis: 1.474 ± 0.041	5.353ValIle: 5.353 ± 0.069	3.726ValLys: 3.726 ± 0.065	6.861ValLeu: 6.861 ± 0.097	2.177ValMet: 2.177 ± 0.051	2.69ValAsn: 2.69 ± 0.077	3.118ValPro: 3.118 ± 0.06	2.584ValGln: 2.584 ± 0.049	3.494ValArg: 3.494 ± 0.062	4.641ValSer: 4.641 ± 0.079	4.431ValThr: 4.431 ± 0.086	4.918ValVal: 4.918 ± 0.083	0.713ValTrp: 0.713 ± 0.03	2.529ValTyr: 2.529 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.711TrpAla: 0.711 ± 0.03	0.139TrpCys: 0.139 ± 0.011	0.6TrpAsp: 0.6 ± 0.027	0.58TrpGlu: 0.58 ± 0.03	0.467TrpPhe: 0.467 ± 0.024	0.675TrpGly: 0.675 ± 0.031	0.307TrpHis: 0.307 ± 0.018	0.715TrpIle: 0.715 ± 0.031	0.689TrpLys: 0.689 ± 0.027	0.993TrpLeu: 0.993 ± 0.04	0.305TrpMet: 0.305 ± 0.02	0.553TrpAsn: 0.553 ± 0.029	0.283TrpPro: 0.283 ± 0.018	0.591TrpGln: 0.591 ± 0.03	0.479TrpArg: 0.479 ± 0.024	0.503TrpSer: 0.503 ± 0.024	0.43TrpThr: 0.43 ± 0.026	0.527TrpVal: 0.527 ± 0.025	0.118TrpTrp: 0.118 ± 0.011	0.357TrpTyr: 0.357 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.973TyrAla: 2.973 ± 0.061	0.577TyrCys: 0.577 ± 0.025	2.362TyrAsp: 2.362 ± 0.066	2.155TyrGlu: 2.155 ± 0.048	1.605TyrPhe: 1.605 ± 0.044	3.089TyrGly: 3.089 ± 0.064	0.948TyrHis: 0.948 ± 0.031	2.691TyrIle: 2.691 ± 0.053	1.908TyrLys: 1.908 ± 0.042	3.273TyrLeu: 3.273 ± 0.069	1.126TyrMet: 1.126 ± 0.04	1.392TyrAsn: 1.392 ± 0.043	1.451TyrPro: 1.451 ± 0.043	1.337TyrGln: 1.337 ± 0.039	1.778TyrArg: 1.778 ± 0.05	1.962TyrSer: 1.962 ± 0.046	2.34TyrThr: 2.34 ± 0.052	2.424TyrVal: 2.424 ± 0.049	0.404TyrTrp: 0.404 ± 0.025	1.385TyrTyr: 1.385 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2843 proteins (863096 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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