Sunguru virus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A023T1K6|A0A023T1K6_9RHAB GDP polyribonucleotidyltransferase OS=Sunguru virus OX=1491491 PE=4 SV=1
MM1 pKa = 7.73 IDD3 pKa = 3.29 EE4 pKa = 4.46 STTDD8 pKa = 3.69 RR9 pKa = 11.84 VCGIQKK15 pKa = 10.54 AIAGEE20 pKa = 4.14 QCHH23 pKa = 6.85 DD24 pKa = 3.88 GSDD27 pKa = 4.08 HH28 pKa = 6.89 ILVGPNPEE36 pKa = 4.92 DD37 pKa = 3.17 IGVVGGSINQSPNMDD52 pKa = 4.26 PLATSQAGFSRR63 pKa = 11.84 NWIKK67 pKa = 11.22 SSMFLPWDD75 pKa = 3.33 SDD77 pKa = 3.38 TTARR81 pKa = 11.84 NDD83 pKa = 3.47 FVDD86 pKa = 3.36 PRR88 pKa = 11.84 GEE90 pKa = 4.07 VSSDD94 pKa = 2.81 RR95 pKa = 11.84 SRR97 pKa = 11.84 EE98 pKa = 4.09 EE99 pKa = 3.78 NSKK102 pKa = 8.72 STPRR106 pKa = 11.84 AVQSDD111 pKa = 3.94 RR112 pKa = 11.84 NSPSGEE118 pKa = 3.37 RR119 pKa = 11.84 DD120 pKa = 3.16 GNNIQFKK127 pKa = 9.97 RR128 pKa = 11.84 GWDD131 pKa = 3.37 AAMTAMEE138 pKa = 4.82 AEE140 pKa = 5.18 FKK142 pKa = 10.58 RR143 pKa = 11.84 LRR145 pKa = 11.84 LNLSIISSLNKK156 pKa = 9.0 LTLVPIPEE164 pKa = 4.16 LTKK167 pKa = 10.15 RR168 pKa = 11.84 DD169 pKa = 3.67 QADD172 pKa = 3.44 QEE174 pKa = 4.59 SEE176 pKa = 4.38 KK177 pKa = 10.95 EE178 pKa = 3.85 DD179 pKa = 3.65 DD180 pKa = 3.93 SEE182 pKa = 4.75 EE183 pKa = 4.17 EE184 pKa = 4.14 EE185 pKa = 4.32 EE186 pKa = 4.29 EE187 pKa = 4.12 QFSKK191 pKa = 11.12 SKK193 pKa = 10.22 FVKK196 pKa = 10.15 LVNRR200 pKa = 11.84 GCLKK204 pKa = 9.89 YY205 pKa = 10.27 KK206 pKa = 9.97 GRR208 pKa = 11.84 KK209 pKa = 5.29 VTSDD213 pKa = 2.98 RR214 pKa = 11.84 VDD216 pKa = 3.34 PTVLQQLKK224 pKa = 10.81 GPMSLKK230 pKa = 10.55 DD231 pKa = 3.08 WLKK234 pKa = 10.89 NVLVV238 pKa = 4.03
Molecular weight: 26.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.128
IPC2_protein 5.092
IPC_protein 5.003
Toseland 4.863
ProMoST 5.105
Dawson 4.952
Bjellqvist 5.092
Wikipedia 4.825
Rodwell 4.851
Grimsley 4.774
Solomon 4.952
Lehninger 4.914
Nozaki 5.067
DTASelect 5.232
Thurlkill 4.863
EMBOSS 4.851
Sillero 5.13
Patrickios 4.635
IPC_peptide 4.965
IPC2_peptide 5.118
IPC2.peptide.svr19 5.183
Protein with the highest isoelectric point:
>tr|A0A023T456|A0A023T456_9RHAB Small hydrophobic protein OS=Sunguru virus OX=1491491 PE=4 SV=1
MM1 pKa = 7.54 SFWNWFRR8 pKa = 11.84 FKK10 pKa = 10.97 KK11 pKa = 10.06 PDD13 pKa = 3.46 LPEE16 pKa = 4.22 SLKK19 pKa = 9.39 VTPVNYY25 pKa = 9.81 GKK27 pKa = 9.77 PYY29 pKa = 9.97 DD30 pKa = 4.58 CYY32 pKa = 11.06 DD33 pKa = 3.0 ISLSVEE39 pKa = 3.9 LFVLEE44 pKa = 5.01 EE45 pKa = 4.21 GNKK48 pKa = 9.51 DD49 pKa = 3.28 LKK51 pKa = 10.99 YY52 pKa = 10.27 NQIIKK57 pKa = 10.57 ACIEE61 pKa = 4.62 SYY63 pKa = 9.96 TGPAEE68 pKa = 3.84 QEE70 pKa = 3.98 AFNVISWLLAGSTLNVKK87 pKa = 10.19 SNSRR91 pKa = 11.84 KK92 pKa = 9.6 RR93 pKa = 11.84 LQTTGRR99 pKa = 11.84 VKK101 pKa = 10.78 FKK103 pKa = 11.18 YY104 pKa = 7.45 QTCNNSLRR112 pKa = 11.84 SRR114 pKa = 11.84 FQYY117 pKa = 10.75 NRR119 pKa = 11.84 TVPIFLRR126 pKa = 11.84 GWKK129 pKa = 9.84 FLLTVEE135 pKa = 4.8 FNGHH139 pKa = 5.64 PCEE142 pKa = 5.0 DD143 pKa = 4.12 GNDD146 pKa = 3.2 ISATLKK152 pKa = 11.04 DD153 pKa = 4.44 KK154 pKa = 11.16 LDD156 pKa = 3.48 QLEE159 pKa = 4.16 DD160 pKa = 3.84 FRR162 pKa = 11.84 TSSIGFNARR171 pKa = 11.84 GKK173 pKa = 10.96 LMLYY177 pKa = 10.38
Molecular weight: 20.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.511
IPC2_protein 8.39
IPC_protein 8.331
Toseland 8.829
ProMoST 8.902
Dawson 9.18
Bjellqvist 9.121
Wikipedia 9.311
Rodwell 9.326
Grimsley 9.107
Solomon 9.311
Lehninger 9.282
Nozaki 9.268
DTASelect 8.99
Thurlkill 9.092
EMBOSS 9.326
Sillero 9.297
Patrickios 4.571
IPC_peptide 9.297
IPC2_peptide 7.878
IPC2.peptide.svr19 7.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3706
78
2087
529.4
60.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.155 ± 0.529
1.619 ± 0.197
6.017 ± 0.558
5.801 ± 0.556
5.208 ± 0.512
5.801 ± 0.19
1.916 ± 0.455
7.717 ± 0.616
7.151 ± 0.496
10.227 ± 1.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.294 ± 0.367
5.451 ± 0.275
4.236 ± 0.182
3.562 ± 0.646
5.154 ± 0.25
8.311 ± 0.336
4.722 ± 0.312
5.559 ± 0.201
1.97 ± 0.173
3.13 ± 0.504
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here