Amino acid dipepetide frequency for Ruminococcaceae bacterium KH2T8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.169AlaAla: 7.169 ± 0.128
1.175AlaCys: 1.175 ± 0.04
5.394AlaAsp: 5.394 ± 0.084
5.195AlaGlu: 5.195 ± 0.096
3.407AlaPhe: 3.407 ± 0.07
5.712AlaGly: 5.712 ± 0.095
1.063AlaHis: 1.063 ± 0.036
5.299AlaIle: 5.299 ± 0.097
4.037AlaLys: 4.037 ± 0.096
6.851AlaLeu: 6.851 ± 0.101
2.26AlaMet: 2.26 ± 0.052
2.483AlaAsn: 2.483 ± 0.06
2.53AlaPro: 2.53 ± 0.078
1.984AlaGln: 1.984 ± 0.054
2.93AlaArg: 2.93 ± 0.063
4.823AlaSer: 4.823 ± 0.071
3.746AlaThr: 3.746 ± 0.084
6.196AlaVal: 6.196 ± 0.1
0.54AlaTrp: 0.54 ± 0.028
2.766AlaTyr: 2.766 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
1.178CysAla: 1.178 ± 0.037
0.285CysCys: 0.285 ± 0.019
1.106CysAsp: 1.106 ± 0.038
0.993CysGlu: 0.993 ± 0.033
0.731CysPhe: 0.731 ± 0.032
1.561CysGly: 1.561 ± 0.053
0.246CysHis: 0.246 ± 0.016
1.096CysIle: 1.096 ± 0.037
0.709CysLys: 0.709 ± 0.029
1.178CysLeu: 1.178 ± 0.035
0.4CysMet: 0.4 ± 0.026
0.611CysAsn: 0.611 ± 0.027
0.652CysPro: 0.652 ± 0.034
0.264CysGln: 0.264 ± 0.018
0.634CysArg: 0.634 ± 0.033
1.084CysSer: 1.084 ± 0.035
0.76CysThr: 0.76 ± 0.029
1.105CysVal: 1.105 ± 0.039
0.128CysTrp: 0.128 ± 0.012
0.628CysTyr: 0.628 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.725AspAla: 4.725 ± 0.08
0.891AspCys: 0.891 ± 0.038
4.696AspAsp: 4.696 ± 0.086
5.571AspGlu: 5.571 ± 0.086
2.763AspPhe: 2.763 ± 0.062
5.162AspGly: 5.162 ± 0.102
1.199AspHis: 1.199 ± 0.041
5.82AspIle: 5.82 ± 0.086
4.253AspLys: 4.253 ± 0.08
5.66AspLeu: 5.66 ± 0.087
1.984AspMet: 1.984 ± 0.054
2.979AspAsn: 2.979 ± 0.065
2.953AspPro: 2.953 ± 0.068
1.564AspGln: 1.564 ± 0.044
3.027AspArg: 3.027 ± 0.059
4.218AspSer: 4.218 ± 0.071
3.724AspThr: 3.724 ± 0.078
4.323AspVal: 4.323 ± 0.074
0.616AspTrp: 0.616 ± 0.03
3.265AspTyr: 3.265 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
5.386GluAla: 5.386 ± 0.095
0.855GluCys: 0.855 ± 0.031
5.109GluAsp: 5.109 ± 0.095
6.133GluGlu: 6.133 ± 0.106
2.902GluPhe: 2.902 ± 0.057
4.602GluGly: 4.602 ± 0.081
1.259GluHis: 1.259 ± 0.037
5.439GluIle: 5.439 ± 0.091
4.535GluLys: 4.535 ± 0.096
6.216GluLeu: 6.216 ± 0.099
2.019GluMet: 2.019 ± 0.046
3.186GluAsn: 3.186 ± 0.068
2.061GluPro: 2.061 ± 0.053
1.994GluGln: 1.994 ± 0.052
3.099GluArg: 3.099 ± 0.071
3.935GluSer: 3.935 ± 0.064
3.808GluThr: 3.808 ± 0.081
4.138GluVal: 4.138 ± 0.075
0.596GluTrp: 0.596 ± 0.03
3.456GluTyr: 3.456 ± 0.075
0.0GluXaa: 0.0 ± 0.0
Phe
3.331PheAla: 3.331 ± 0.059
0.8PheCys: 0.8 ± 0.033
3.34PheAsp: 3.34 ± 0.066
3.053PheGlu: 3.053 ± 0.06
2.179PhePhe: 2.179 ± 0.06
3.232PheGly: 3.232 ± 0.069
0.651PheHis: 0.651 ± 0.028
3.218PheIle: 3.218 ± 0.077
2.187PheLys: 2.187 ± 0.061
3.484PheLeu: 3.484 ± 0.069
1.268PheMet: 1.268 ± 0.036
1.677PheAsn: 1.677 ± 0.042
1.469PhePro: 1.469 ± 0.044
0.737PheGln: 0.737 ± 0.028
1.775PheArg: 1.775 ± 0.054
3.036PheSer: 3.036 ± 0.068
2.781PheThr: 2.781 ± 0.065
3.249PheVal: 3.249 ± 0.066
0.438PheTrp: 0.438 ± 0.024
1.811PheTyr: 1.811 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
4.742GlyAla: 4.742 ± 0.09
1.215GlyCys: 1.215 ± 0.042
4.361GlyAsp: 4.361 ± 0.078
4.614GlyGlu: 4.614 ± 0.071
3.359GlyPhe: 3.359 ± 0.06
4.662GlyGly: 4.662 ± 0.119
1.262GlyHis: 1.262 ± 0.038
5.988GlyIle: 5.988 ± 0.091
4.839GlyLys: 4.839 ± 0.095
5.551GlyLeu: 5.551 ± 0.086
2.226GlyMet: 2.226 ± 0.049
3.165GlyAsn: 3.165 ± 0.081
1.375GlyPro: 1.375 ± 0.041
1.657GlyGln: 1.657 ± 0.051
3.049GlyArg: 3.049 ± 0.068
4.596GlySer: 4.596 ± 0.086
4.307GlyThr: 4.307 ± 0.115
4.919GlyVal: 4.919 ± 0.086
0.723GlyTrp: 0.723 ± 0.03
3.468GlyTyr: 3.468 ± 0.069
0.0GlyXaa: 0.0 ± 0.0
His
1.047HisAla: 1.047 ± 0.033
0.27HisCys: 0.27 ± 0.02
1.072HisAsp: 1.072 ± 0.037
1.153HisGlu: 1.153 ± 0.038
0.771HisPhe: 0.771 ± 0.026
1.249HisGly: 1.249 ± 0.039
0.351HisHis: 0.351 ± 0.026
1.301HisIle: 1.301 ± 0.037
0.915HisLys: 0.915 ± 0.031
1.244HisLeu: 1.244 ± 0.036
0.456HisMet: 0.456 ± 0.025
0.673HisAsn: 0.673 ± 0.026
0.799HisPro: 0.799 ± 0.029
0.357HisGln: 0.357 ± 0.02
0.627HisArg: 0.627 ± 0.026
0.936HisSer: 0.936 ± 0.031
0.889HisThr: 0.889 ± 0.035
1.04HisVal: 1.04 ± 0.037
0.147HisTrp: 0.147 ± 0.014
0.704HisTyr: 0.704 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.186IleAla: 6.186 ± 0.092
1.38IleCys: 1.38 ± 0.038
5.43IleAsp: 5.43 ± 0.081
5.067IleGlu: 5.067 ± 0.095
3.097IlePhe: 3.097 ± 0.073
5.191IleGly: 5.191 ± 0.094
1.06IleHis: 1.06 ± 0.031
5.155IleIle: 5.155 ± 0.096
4.267IleLys: 4.267 ± 0.088
6.773IleLeu: 6.773 ± 0.099
2.008IleMet: 2.008 ± 0.047
3.279IleAsn: 3.279 ± 0.068
3.29IlePro: 3.29 ± 0.061
1.317IleGln: 1.317 ± 0.04
3.902IleArg: 3.902 ± 0.074
5.696IleSer: 5.696 ± 0.098
4.875IleThr: 4.875 ± 0.089
5.527IleVal: 5.527 ± 0.087
0.623IleTrp: 0.623 ± 0.027
2.911IleTyr: 2.911 ± 0.064
0.0IleXaa: 0.0 ± 0.0
Lys
4.799LysAla: 4.799 ± 0.102
0.717LysCys: 0.717 ± 0.037
4.147LysAsp: 4.147 ± 0.078
4.673LysGlu: 4.673 ± 0.093
1.937LysPhe: 1.937 ± 0.052
3.638LysGly: 3.638 ± 0.065
0.938LysHis: 0.938 ± 0.033
4.655LysIle: 4.655 ± 0.078
4.971LysLys: 4.971 ± 0.094
4.722LysLeu: 4.722 ± 0.083
1.728LysMet: 1.728 ± 0.052
2.826LysAsn: 2.826 ± 0.058
1.805LysPro: 1.805 ± 0.047
1.394LysGln: 1.394 ± 0.04
2.77LysArg: 2.77 ± 0.058
3.153LysSer: 3.153 ± 0.072
3.451LysThr: 3.451 ± 0.075
3.908LysVal: 3.908 ± 0.078
0.523LysTrp: 0.523 ± 0.025
2.394LysTyr: 2.394 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
6.25LeuAla: 6.25 ± 0.096
1.441LeuCys: 1.441 ± 0.045
5.298LeuAsp: 5.298 ± 0.079
5.0LeuGlu: 5.0 ± 0.08
3.829LeuPhe: 3.829 ± 0.087
5.379LeuGly: 5.379 ± 0.082
1.307LeuHis: 1.307 ± 0.039
7.107LeuIle: 7.107 ± 0.112
5.189LeuLys: 5.189 ± 0.095
7.286LeuLeu: 7.286 ± 0.127
2.708LeuMet: 2.708 ± 0.062
3.415LeuAsn: 3.415 ± 0.063
3.386LeuPro: 3.386 ± 0.062
1.892LeuGln: 1.892 ± 0.051
3.625LeuArg: 3.625 ± 0.077
6.234LeuSer: 6.234 ± 0.1
5.141LeuThr: 5.141 ± 0.083
5.427LeuVal: 5.427 ± 0.097
0.697LeuTrp: 0.697 ± 0.027
3.337LeuTyr: 3.337 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.294MetAla: 2.294 ± 0.058
0.434MetCys: 0.434 ± 0.019
1.678MetAsp: 1.678 ± 0.043
1.603MetGlu: 1.603 ± 0.045
1.201MetPhe: 1.201 ± 0.042
1.872MetGly: 1.872 ± 0.053
0.467MetHis: 0.467 ± 0.022
2.242MetIle: 2.242 ± 0.05
2.08MetLys: 2.08 ± 0.046
2.692MetLeu: 2.692 ± 0.067
0.841MetMet: 0.841 ± 0.033
1.483MetAsn: 1.483 ± 0.035
1.186MetPro: 1.186 ± 0.039
0.711MetGln: 0.711 ± 0.031
1.355MetArg: 1.355 ± 0.041
2.119MetSer: 2.119 ± 0.047
1.848MetThr: 1.848 ± 0.047
1.74MetVal: 1.74 ± 0.047
0.261MetTrp: 0.261 ± 0.018
1.062MetTyr: 1.062 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
2.992AsnAla: 2.992 ± 0.06
0.651AsnCys: 0.651 ± 0.026
2.973AsnAsp: 2.973 ± 0.062
2.876AsnGlu: 2.876 ± 0.063
1.496AsnPhe: 1.496 ± 0.044
3.636AsnGly: 3.636 ± 0.091
0.569AsnHis: 0.569 ± 0.027
3.657AsnIle: 3.657 ± 0.07
2.321AsnLys: 2.321 ± 0.062
3.049AsnLeu: 3.049 ± 0.062
1.238AsnMet: 1.238 ± 0.038
2.043AsnAsn: 2.043 ± 0.061
1.819AsnPro: 1.819 ± 0.046
0.846AsnGln: 0.846 ± 0.031
1.926AsnArg: 1.926 ± 0.051
2.591AsnSer: 2.591 ± 0.062
2.431AsnThr: 2.431 ± 0.065
3.011AsnVal: 3.011 ± 0.059
0.408AsnTrp: 0.408 ± 0.021
1.794AsnTyr: 1.794 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
2.784ProAla: 2.784 ± 0.07
0.424ProCys: 0.424 ± 0.024
3.045ProAsp: 3.045 ± 0.059
3.47ProGlu: 3.47 ± 0.071
1.612ProPhe: 1.612 ± 0.046
2.283ProGly: 2.283 ± 0.047
0.597ProHis: 0.597 ± 0.024
2.202ProIle: 2.202 ± 0.051
1.678ProLys: 1.678 ± 0.053
2.761ProLeu: 2.761 ± 0.057
0.917ProMet: 0.917 ± 0.028
1.349ProAsn: 1.349 ± 0.04
0.741ProPro: 0.741 ± 0.036
0.918ProGln: 0.918 ± 0.042
1.098ProArg: 1.098 ± 0.041
2.199ProSer: 2.199 ± 0.049
2.025ProThr: 2.025 ± 0.081
3.045ProVal: 3.045 ± 0.069
0.342ProTrp: 0.342 ± 0.02
1.543ProTyr: 1.543 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
1.727GlnAla: 1.727 ± 0.042
0.267GlnCys: 0.267 ± 0.015
1.335GlnAsp: 1.335 ± 0.043
1.602GlnGlu: 1.602 ± 0.049
0.968GlnPhe: 0.968 ± 0.032
1.533GlnGly: 1.533 ± 0.041
0.349GlnHis: 0.349 ± 0.02
1.898GlnIle: 1.898 ± 0.054
1.456GlnLys: 1.456 ± 0.048
1.952GlnLeu: 1.952 ± 0.045
0.809GlnMet: 0.809 ± 0.029
1.075GlnAsn: 1.075 ± 0.041
0.691GlnPro: 0.691 ± 0.033
0.732GlnGln: 0.732 ± 0.03
1.082GlnArg: 1.082 ± 0.038
1.346GlnSer: 1.346 ± 0.041
1.357GlnThr: 1.357 ± 0.038
1.414GlnVal: 1.414 ± 0.045
0.224GlnTrp: 0.224 ± 0.019
1.118GlnTyr: 1.118 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
2.828ArgAla: 2.828 ± 0.06
0.583ArgCys: 0.583 ± 0.027
2.708ArgAsp: 2.708 ± 0.057
3.224ArgGlu: 3.224 ± 0.067
2.035ArgPhe: 2.035 ± 0.048
2.465ArgGly: 2.465 ± 0.06
0.807ArgHis: 0.807 ± 0.03
3.479ArgIle: 3.479 ± 0.074
3.076ArgLys: 3.076 ± 0.067
3.775ArgLeu: 3.775 ± 0.074
1.377ArgMet: 1.377 ± 0.043
2.03ArgAsn: 2.03 ± 0.054
1.363ArgPro: 1.363 ± 0.038
1.152ArgGln: 1.152 ± 0.034
2.175ArgArg: 2.175 ± 0.062
2.741ArgSer: 2.741 ± 0.059
2.25ArgThr: 2.25 ± 0.048
2.667ArgVal: 2.667 ± 0.058
0.37ArgTrp: 0.37 ± 0.02
2.17ArgTyr: 2.17 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
4.703SerAla: 4.703 ± 0.068
1.01SerCys: 1.01 ± 0.036
4.927SerAsp: 4.927 ± 0.081
4.82SerGlu: 4.82 ± 0.074
3.171SerPhe: 3.171 ± 0.067
5.416SerGly: 5.416 ± 0.078
1.009SerHis: 1.009 ± 0.03
4.659SerIle: 4.659 ± 0.076
3.465SerLys: 3.465 ± 0.062
5.394SerLeu: 5.394 ± 0.081
1.931SerMet: 1.931 ± 0.046
2.619SerAsn: 2.619 ± 0.065
1.936SerPro: 1.936 ± 0.051
1.504SerGln: 1.504 ± 0.04
2.77SerArg: 2.77 ± 0.058
4.67SerSer: 4.67 ± 0.097
3.298SerThr: 3.298 ± 0.071
5.018SerVal: 5.018 ± 0.076
0.569SerTrp: 0.569 ± 0.027
2.983SerTyr: 2.983 ± 0.074
0.0SerXaa: 0.0 ± 0.0
Thr
4.795ThrAla: 4.795 ± 0.088
0.822ThrCys: 0.822 ± 0.031
4.215ThrAsp: 4.215 ± 0.083
3.883ThrGlu: 3.883 ± 0.073
2.379ThrPhe: 2.379 ± 0.065
4.651ThrGly: 4.651 ± 0.091
0.918ThrHis: 0.918 ± 0.033
4.089ThrIle: 4.089 ± 0.083
2.782ThrLys: 2.782 ± 0.058
4.911ThrLeu: 4.911 ± 0.082
1.399ThrMet: 1.399 ± 0.041
1.994ThrAsn: 1.994 ± 0.064
2.538ThrPro: 2.538 ± 0.083
1.181ThrGln: 1.181 ± 0.036
2.038ThrArg: 2.038 ± 0.047
3.67ThrSer: 3.67 ± 0.084
3.845ThrThr: 3.845 ± 0.134
4.958ThrVal: 4.958 ± 0.111
0.472ThrTrp: 0.472 ± 0.024
2.583ThrTyr: 2.583 ± 0.103
0.0ThrXaa: 0.0 ± 0.0
Val
5.226ValAla: 5.226 ± 0.087
1.232ValCys: 1.232 ± 0.038
4.411ValAsp: 4.411 ± 0.079
4.114ValGlu: 4.114 ± 0.065
3.541ValPhe: 3.541 ± 0.081
4.164ValGly: 4.164 ± 0.085
1.026ValHis: 1.026 ± 0.035
5.891ValIle: 5.891 ± 0.091
3.696ValLys: 3.696 ± 0.077
6.08ValLeu: 6.08 ± 0.092
2.116ValMet: 2.116 ± 0.044
2.985ValAsn: 2.985 ± 0.062
2.739ValPro: 2.739 ± 0.061
1.376ValGln: 1.376 ± 0.043
3.106ValArg: 3.106 ± 0.062
5.17ValSer: 5.17 ± 0.084
4.56ValThr: 4.56 ± 0.094
4.837ValVal: 4.837 ± 0.081
0.593ValTrp: 0.593 ± 0.027
2.99ValTyr: 2.99 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.531TrpAla: 0.531 ± 0.024
0.142TrpCys: 0.142 ± 0.014
0.634TrpAsp: 0.634 ± 0.03
0.543TrpGlu: 0.543 ± 0.026
0.453TrpPhe: 0.453 ± 0.02
0.677TrpGly: 0.677 ± 0.03
0.216TrpHis: 0.216 ± 0.014
0.622TrpIle: 0.622 ± 0.028
0.51TrpLys: 0.51 ± 0.027
0.801TrpLeu: 0.801 ± 0.032
0.269TrpMet: 0.269 ± 0.019
0.521TrpAsn: 0.521 ± 0.026
0.196TrpPro: 0.196 ± 0.016
0.297TrpGln: 0.297 ± 0.023
0.325TrpArg: 0.325 ± 0.02
0.532TrpSer: 0.532 ± 0.028
0.444TrpThr: 0.444 ± 0.023
0.512TrpVal: 0.512 ± 0.025
0.12TrpTrp: 0.12 ± 0.012
0.47TrpTyr: 0.47 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.868TyrAla: 2.868 ± 0.053
0.757TyrCys: 0.757 ± 0.03
3.516TyrAsp: 3.516 ± 0.098
3.141TyrGlu: 3.141 ± 0.068
1.882TyrPhe: 1.882 ± 0.05
2.994TyrGly: 2.994 ± 0.067
0.705TyrHis: 0.705 ± 0.027
3.218TyrIle: 3.218 ± 0.059
2.183TyrLys: 2.183 ± 0.046
3.568TyrLeu: 3.568 ± 0.078
1.214TyrMet: 1.214 ± 0.043
1.936TyrAsn: 1.936 ± 0.051
1.507TyrPro: 1.507 ± 0.043
1.057TyrGln: 1.057 ± 0.038
1.958TyrArg: 1.958 ± 0.049
3.121TyrSer: 3.121 ± 0.075
2.586TyrThr: 2.586 ± 0.079
2.817TyrVal: 2.817 ± 0.061
0.423TyrTrp: 0.423 ± 0.026
2.2TyrTyr: 2.2 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2593 proteins (889011 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski