Pepper vein yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7FL70|E7FL70_9LUTE Putative P0 protein OS=Pepper vein yellows virus OX=909827 GN=ORF0 PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 4.8MVDD5 pKa = 3.73HH6 pKa = 5.91VTPAVVDD13 pKa = 3.39AHH15 pKa = 7.12DD16 pKa = 4.31RR17 pKa = 11.84FALSLWSHH25 pKa = 7.0PLGAHH30 pKa = 5.67GEE32 pKa = 4.16EE33 pKa = 4.5IEE35 pKa = 4.47DD36 pKa = 4.35DD37 pKa = 3.64EE38 pKa = 4.7MEE40 pKa = 4.97AGTEE44 pKa = 3.77EE45 pKa = 4.63AEE47 pKa = 4.17IEE49 pKa = 4.2LEE51 pKa = 4.12EE52 pKa = 4.51GRR54 pKa = 11.84ATAKK58 pKa = 10.45LSSSTRR64 pKa = 11.84TQSRR68 pKa = 11.84IVPQDD73 pKa = 3.41LSPSGRR79 pKa = 11.84LYY81 pKa = 10.52QRR83 pKa = 11.84ASRR86 pKa = 11.84FQVEE90 pKa = 4.53FSKK93 pKa = 9.45PTMNIRR99 pKa = 11.84SQWSTYY105 pKa = 10.07ASSVNLLPQRR115 pKa = 11.84RR116 pKa = 11.84APSLTSWTPTASLLVSNPPCVSSPSPKK143 pKa = 9.64AGKK146 pKa = 10.0RR147 pKa = 11.84LFGLRR152 pKa = 11.84RR153 pKa = 11.84LTGG156 pKa = 3.29

Molecular weight: 17.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7FL74|E7FL74_9LUTE Putative P3 protein OS=Pepper vein yellows virus OX=909827 GN=ORF3 PE=4 SV=1
MM1 pKa = 6.77NTGGVRR7 pKa = 11.84RR8 pKa = 11.84NNNGNGGSRR17 pKa = 11.84NTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84PRR26 pKa = 11.84QVRR29 pKa = 11.84PVVVVAPPGRR39 pKa = 11.84TRR41 pKa = 11.84RR42 pKa = 11.84GNRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84NGGRR52 pKa = 11.84NRR54 pKa = 11.84RR55 pKa = 11.84SRR57 pKa = 11.84NRR59 pKa = 11.84VGGRR63 pKa = 11.84SSNSEE68 pKa = 3.69TFIFNKK74 pKa = 10.42DD75 pKa = 3.59SIKK78 pKa = 10.76DD79 pKa = 3.63SSSGSVTFGPSLSEE93 pKa = 4.12SVALSGGVLKK103 pKa = 10.54AYY105 pKa = 10.22HH106 pKa = 6.68EE107 pKa = 4.5YY108 pKa = 10.51KK109 pKa = 9.32ITMVNIRR116 pKa = 11.84FVSEE120 pKa = 4.1SSSTAEE126 pKa = 3.6GSIAYY131 pKa = 9.39EE132 pKa = 4.08LDD134 pKa = 3.39PHH136 pKa = 7.02CKK138 pKa = 8.88LTSLQSTLRR147 pKa = 11.84KK148 pKa = 9.77FPVTKK153 pKa = 10.36GGQATFRR160 pKa = 11.84ASQINGVEE168 pKa = 3.87WHH170 pKa = 6.98DD171 pKa = 3.52TSEE174 pKa = 4.07DD175 pKa = 3.53QFRR178 pKa = 11.84LLYY181 pKa = 10.37RR182 pKa = 11.84GNGTKK187 pKa = 10.23NVAAGFFQIRR197 pKa = 11.84FTVQLHH203 pKa = 5.06NPKK206 pKa = 10.54

Molecular weight: 22.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3086

156

1085

514.3

57.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.384 ± 0.376

1.458 ± 0.295

3.856 ± 0.446

6.708 ± 0.464

4.569 ± 0.398

7.453 ± 0.425

2.139 ± 0.317

3.597 ± 0.305

5.768 ± 0.498

7.777 ± 0.982

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.977 ± 0.301

3.921 ± 0.603

6.935 ± 0.593

3.921 ± 0.154

6.967 ± 1.031

9.559 ± 0.393

6.092 ± 0.404

6.578 ± 0.445

1.977 ± 0.207

2.333 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski