Candidatus Marinimicrobia bacterium PRS2

Taxonomy: cellular organisms; Bacteria; FCB group; Candidatus Marinimicrobia

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3M7KIF0|A0A3M7KIF0_9BACT Uncharacterized protein OS=Candidatus Marinimicrobia bacterium PRS2 OX=2483458 GN=EB821_05875 PE=4 SV=1
SS1 pKa = 6.79NGGLGGSTVIATVTVNPVNDD21 pKa = 3.86EE22 pKa = 4.03PSFEE26 pKa = 4.28YY27 pKa = 10.92TNVVAVNEE35 pKa = 4.05DD36 pKa = 3.32SGLYY40 pKa = 7.63EE41 pKa = 4.5ASWAFNISVGPDD53 pKa = 3.25NEE55 pKa = 4.47SDD57 pKa = 3.59QIPTFEE63 pKa = 5.38IISNDD68 pKa = 3.2HH69 pKa = 6.33EE70 pKa = 5.51SYY72 pKa = 10.98FSIQPSISEE81 pKa = 4.23LGVLTFTPADD91 pKa = 3.8NQHH94 pKa = 6.04GEE96 pKa = 4.09AIITVRR102 pKa = 11.84LTDD105 pKa = 3.55NGGTEE110 pKa = 3.97NGGVNTYY117 pKa = 11.09DD118 pKa = 3.9DD119 pKa = 3.41QTFTIKK125 pKa = 10.63LNPIADD131 pKa = 4.08TPNITDD137 pKa = 3.81AEE139 pKa = 4.16TDD141 pKa = 3.42EE142 pKa = 6.81DD143 pKa = 4.04IMSSSGLVITRR154 pKa = 11.84NAVDD158 pKa = 3.77SEE160 pKa = 4.52EE161 pKa = 4.12VTHH164 pKa = 7.03FKK166 pKa = 9.2ITGITNGTLYY176 pKa = 10.68QNNGSTSIANNEE188 pKa = 4.26FITFTEE194 pKa = 4.74GNTGLKK200 pKa = 9.9FDD202 pKa = 5.24PDD204 pKa = 3.11EE205 pKa = 5.15DD206 pKa = 5.24YY207 pKa = 11.3YY208 pKa = 11.73GSGFITIQASVSSSNGGLGGSTVIATVTVNPEE240 pKa = 3.41NDD242 pKa = 3.5GPYY245 pKa = 10.26DD246 pKa = 3.8AEE248 pKa = 6.0AGDD251 pKa = 5.2DD252 pKa = 4.07IYY254 pKa = 11.41DD255 pKa = 4.15ILPSDD260 pKa = 3.82ATTIDD265 pKa = 3.28ITILDD270 pKa = 4.11VSSDD274 pKa = 4.41DD275 pKa = 3.66IDD277 pKa = 6.61DD278 pKa = 4.54DD279 pKa = 4.82VSVSHH284 pKa = 6.76TYY286 pKa = 10.1SWTLIDD292 pKa = 5.1ANNGDD297 pKa = 4.61IIAEE301 pKa = 3.98ATEE304 pKa = 4.06INPTFALKK312 pKa = 10.39AGHH315 pKa = 5.79YY316 pKa = 8.9QFEE319 pKa = 4.7LTVTDD324 pKa = 3.49TTTNDD329 pKa = 2.94SNIYY333 pKa = 10.86GDD335 pKa = 3.52ITDD338 pKa = 4.11TDD340 pKa = 4.02TIDD343 pKa = 3.76IYY345 pKa = 10.68IGQPGVDD352 pKa = 3.08ISSNPVFVTYY362 pKa = 11.05DD363 pKa = 3.28DD364 pKa = 3.34VAYY367 pKa = 9.94IDD369 pKa = 3.32IVYY372 pKa = 10.27FEE374 pKa = 5.05HH375 pKa = 7.44EE376 pKa = 4.54YY377 pKa = 11.46ASVTQNGDD385 pKa = 3.37QIVISVPIEE394 pKa = 3.97YY395 pKa = 10.11QNKK398 pKa = 7.66FQFIAGQIIPQDD410 pKa = 3.42ALEE413 pKa = 4.08IQIGYY418 pKa = 9.65SGDD421 pKa = 3.42VTDD424 pKa = 5.71ISIEE428 pKa = 4.32SITTSAITINVVTSHH443 pKa = 6.41ISEE446 pKa = 3.99NFYY449 pKa = 10.82IKK451 pKa = 10.57LKK453 pKa = 10.19SVEE456 pKa = 4.19VNPIDD461 pKa = 3.74VTSDD465 pKa = 3.41FSLEE469 pKa = 4.06IEE471 pKa = 4.22NDD473 pKa = 3.03NYY475 pKa = 11.46LPILKK480 pKa = 9.91SATEE484 pKa = 3.82ISIGNPKK491 pKa = 8.31ITTITDD497 pKa = 3.35QVLITANPNEE507 pKa = 4.23KK508 pKa = 9.44MLDD511 pKa = 3.35IIVYY515 pKa = 9.56EE516 pKa = 4.46DD517 pKa = 3.7EE518 pKa = 4.34SVAAINTDD526 pKa = 3.61DD527 pKa = 5.84DD528 pKa = 3.95IVIQFPSTLEE538 pKa = 4.15NNVKK542 pKa = 9.8WIQVPTASSNSEE554 pKa = 4.1KK555 pKa = 10.49IGTITIDD562 pKa = 3.38SSDD565 pKa = 3.77STKK568 pKa = 11.16LLIDD572 pKa = 4.36VISDD576 pKa = 3.35FFAGEE581 pKa = 4.18SLILTDD587 pKa = 5.28GYY589 pKa = 11.02VQVGDD594 pKa = 3.89IEE596 pKa = 5.24SNEE599 pKa = 3.71DD600 pKa = 3.26DD601 pKa = 4.07YY602 pKa = 12.12YY603 pKa = 11.59KK604 pKa = 10.54IIAYY608 pKa = 10.21VNGRR612 pKa = 11.84NQSLDD617 pKa = 3.61VEE619 pKa = 4.76CDD621 pKa = 2.8NSIIVTSPEE630 pKa = 3.59LSFASDD636 pKa = 2.57QHH638 pKa = 6.88YY639 pKa = 10.64VVNDD643 pKa = 3.61SAVSFPTITLTEE655 pKa = 4.1SSLIPSIYY663 pKa = 10.53SEE665 pKa = 4.35VQDD668 pKa = 4.23SIYY671 pKa = 10.84LRR673 pKa = 11.84FDD675 pKa = 3.68NPCMTWEE682 pKa = 3.84QVPIEE687 pKa = 4.05EE688 pKa = 5.28LNFSGYY694 pKa = 10.85NGVGDD699 pKa = 4.14IIYY702 pKa = 10.19RR703 pKa = 11.84EE704 pKa = 4.0NNQTVIINISEE715 pKa = 4.53PFTISQSLNIDD726 pKa = 3.48GLKK729 pKa = 10.94VNICNTNFYY738 pKa = 10.71NGAGLEE744 pKa = 3.98YY745 pKa = 10.32SIRR748 pKa = 11.84SDD750 pKa = 3.12PTFYY754 pKa = 11.05LHH756 pKa = 7.88DD757 pKa = 4.44NGEE760 pKa = 4.72HH761 pKa = 5.87ICKK764 pKa = 9.91SSKK767 pKa = 10.44PIITAIEE774 pKa = 3.96DD775 pKa = 3.69EE776 pKa = 4.56YY777 pKa = 12.0VNINSISEE785 pKa = 4.46MITKK789 pKa = 10.24LIIKK793 pKa = 9.02EE794 pKa = 4.06DD795 pKa = 3.61SVLPIINTTRR805 pKa = 11.84KK806 pKa = 9.0IVIWAPDD813 pKa = 3.81PLIWDD818 pKa = 3.93SNISLVSLEE827 pKa = 4.37GQGKK831 pKa = 7.37TKK833 pKa = 10.46VSPEE837 pKa = 3.76LTYY840 pKa = 11.52SNDD843 pKa = 3.34NKK845 pKa = 10.32QLQLDD850 pKa = 3.76ITDD853 pKa = 3.88SFSAFDD859 pKa = 3.62SLVISGMRR867 pKa = 11.84VKK869 pKa = 10.77KK870 pKa = 10.48DD871 pKa = 3.21GNYY874 pKa = 10.5SGDD877 pKa = 3.78FASSAHH883 pKa = 6.0FKK885 pKa = 10.31MSLPEE890 pKa = 5.04SIDD893 pKa = 3.16SFYY896 pKa = 10.51PIHH899 pKa = 7.17LDD901 pKa = 3.3NSSSDD906 pKa = 3.3LSIIYY911 pKa = 10.35SSAHH915 pKa = 6.28LEE917 pKa = 4.23FEE919 pKa = 4.47SSQGANKK926 pKa = 9.92IVFIKK931 pKa = 10.77GGANSEE937 pKa = 4.35YY938 pKa = 10.72FNVTIFGGSLSNSINIDD955 pKa = 3.16TDD957 pKa = 3.22IKK959 pKa = 11.38LMLPTFLNVHH969 pKa = 4.77WVEE972 pKa = 4.12NQSAQIDD979 pKa = 4.32PGTPAHH985 pKa = 7.32IINNVASINSLAILPNTNNKK1005 pKa = 8.5VLNLNVLEE1013 pKa = 4.63AFSINDD1019 pKa = 3.63SIKK1022 pKa = 10.37INNLSVEE1029 pKa = 4.27NNTTVNDD1036 pKa = 3.71MSKK1039 pKa = 9.94IDD1041 pKa = 4.14YY1042 pKa = 10.73LSLLQGDD1049 pKa = 4.23YY1050 pKa = 11.24GSNNHH1055 pKa = 7.43AINQADD1061 pKa = 4.15SLIVAAPQMMIAKK1074 pKa = 9.56DD1075 pKa = 3.14ITVITGALEE1084 pKa = 4.51NINIPPITIVDD1095 pKa = 3.75DD1096 pKa = 3.78TLEE1099 pKa = 3.93QVLNIGNNNFYY1110 pKa = 10.69YY1111 pKa = 10.78LSLEE1115 pKa = 4.58GISNYY1120 pKa = 9.73EE1121 pKa = 3.57WDD1123 pKa = 4.75SGFTCLNMLCTLSDD1137 pKa = 3.11NKK1139 pKa = 10.07TIKK1142 pKa = 10.45FLIQSSGTLTISNLKK1157 pKa = 9.61LKK1159 pKa = 10.71NVTFDD1164 pKa = 3.61NTDD1167 pKa = 3.11DD1168 pKa = 4.01AYY1170 pKa = 11.43RR1171 pKa = 11.84LVLKK1175 pKa = 10.65DD1176 pKa = 4.17NNTKK1180 pKa = 10.08MIAKK1184 pKa = 9.69SINTIKK1190 pKa = 10.04TGMPKK1195 pKa = 9.13IDD1197 pKa = 5.8LITGVIWVDD1206 pKa = 3.39LDD1208 pKa = 4.0SIKK1211 pKa = 10.82AISTLKK1217 pKa = 10.19IIEE1220 pKa = 4.74SINHH1224 pKa = 6.13ISSDD1228 pKa = 3.7SLKK1231 pKa = 10.91LYY1233 pKa = 10.4FDD1235 pKa = 3.57KK1236 pKa = 11.11SYY1238 pKa = 11.27LKK1240 pKa = 9.84WDD1242 pKa = 3.69QQSSVQSTINYY1253 pKa = 7.23GFPSEE1258 pKa = 4.28GEE1260 pKa = 4.09LYY1262 pKa = 10.41INQYY1266 pKa = 11.0EE1267 pKa = 4.23NGSNSAFDD1275 pKa = 4.27LSAFNFLITSDD1286 pKa = 3.67VFKK1289 pKa = 10.78DD1290 pKa = 2.97ITQADD1295 pKa = 3.57TVMNLNCVLMDD1306 pKa = 4.34EE1307 pKa = 4.78YY1308 pKa = 11.67NQFTITHH1315 pKa = 6.9PIILNFDD1322 pKa = 3.99PLISDD1327 pKa = 4.41VIPLGGTTWDD1337 pKa = 3.65SLTLSVIINEE1347 pKa = 4.21NFLEE1351 pKa = 5.14DD1352 pKa = 3.83EE1353 pKa = 4.44GTDD1356 pKa = 3.53LSSIRR1361 pKa = 11.84PTLLLLDD1368 pKa = 4.49GEE1370 pKa = 5.35GDD1372 pKa = 3.48TSNYY1376 pKa = 10.35DD1377 pKa = 2.86IDD1379 pKa = 4.25VSFTQTAAQGDD1390 pKa = 4.44FPEE1393 pKa = 4.68GVYY1396 pKa = 9.07TAEE1399 pKa = 4.83FYY1401 pKa = 11.06FPDD1404 pKa = 3.7SLIDD1408 pKa = 3.46ILTSKK1413 pKa = 10.68QEE1415 pKa = 4.01NNDD1418 pKa = 2.88IDD1420 pKa = 4.98LLLSLTDD1427 pKa = 3.36IDD1429 pKa = 4.82SNALSRR1435 pKa = 11.84SNSSKK1440 pKa = 11.15DD1441 pKa = 3.12NWKK1444 pKa = 8.89LTPISLGWSEE1454 pKa = 5.87DD1455 pKa = 2.91ITNIYY1460 pKa = 10.01LDD1462 pKa = 3.16QSLRR1466 pKa = 11.84YY1467 pKa = 9.75LNPDD1471 pKa = 3.03SNDD1474 pKa = 2.95IVFTLPEE1481 pKa = 5.16PISTTCEE1488 pKa = 3.23IRR1490 pKa = 11.84INNLVSDD1497 pKa = 4.07STYY1500 pKa = 11.11EE1501 pKa = 4.0LGTMAINEE1509 pKa = 4.15NSTITIEE1516 pKa = 4.13GTQIQNALGRR1526 pKa = 11.84DD1527 pKa = 3.09ADD1529 pKa = 4.41GIYY1532 pKa = 10.34DD1533 pKa = 5.27IILQDD1538 pKa = 3.3KK1539 pKa = 10.53SGGSFSFPFIKK1550 pKa = 10.25RR1551 pKa = 11.84IIIDD1555 pKa = 3.7TSPPQFISINPTTDD1569 pKa = 2.9VFDD1572 pKa = 4.36SGWNEE1577 pKa = 3.81NSVHH1581 pKa = 7.11SITDD1585 pKa = 2.99QDD1587 pKa = 4.63KK1588 pKa = 11.0IILTFKK1594 pKa = 10.14DD1595 pKa = 4.09YY1596 pKa = 11.03PIMTTVDD1603 pKa = 3.58SIFIYY1608 pKa = 10.78NSGIVSAMAPGGYY1621 pKa = 9.88LFNDD1625 pKa = 4.28SIDD1628 pKa = 3.47IQISFEE1634 pKa = 4.16LTDD1637 pKa = 3.66SNGNDD1642 pKa = 3.11TSYY1645 pKa = 10.81TFLNTDD1651 pKa = 3.37EE1652 pKa = 5.41NGLLDD1657 pKa = 4.44FYY1659 pKa = 11.19KK1660 pKa = 10.75EE1661 pKa = 4.31SIIKK1665 pKa = 10.68LSDD1668 pKa = 3.22HH1669 pKa = 5.28QLEE1672 pKa = 4.63GKK1674 pKa = 10.17NSIIINYY1681 pKa = 8.55VLSDD1685 pKa = 3.47HH1686 pKa = 7.26AGNEE1690 pKa = 3.75QNYY1693 pKa = 7.55TIKK1696 pKa = 10.8YY1697 pKa = 8.05VLYY1700 pKa = 9.97NASDD1704 pKa = 3.4IQGLVDD1710 pKa = 6.26DD1711 pKa = 5.25IFNFPNPFSSVDD1723 pKa = 2.79EE1724 pKa = 4.37GTRR1727 pKa = 11.84IRR1729 pKa = 11.84YY1730 pKa = 9.47SLTQDD1735 pKa = 3.23GNQGKK1740 pKa = 9.3YY1741 pKa = 7.46VVFNAKK1747 pKa = 10.4GEE1749 pKa = 4.25LVYY1752 pKa = 10.41HH1753 pKa = 6.19YY1754 pKa = 10.03SLSSSEE1760 pKa = 4.19LQQGTHH1766 pKa = 6.09TIYY1769 pKa = 10.47WDD1771 pKa = 3.28GHH1773 pKa = 4.78YY1774 pKa = 11.08LNNNYY1779 pKa = 10.28LLATGVYY1786 pKa = 10.21FGFLDD1791 pKa = 4.37IDD1793 pKa = 4.11DD1794 pKa = 5.66KK1795 pKa = 11.12IAKK1798 pKa = 9.63HH1799 pKa = 6.23KK1800 pKa = 10.38IAIINN1805 pKa = 3.58

Molecular weight:
199.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3M7KIZ1|A0A3M7KIZ1_9BACT Electron transfer flavoprotein-ubiquinone oxidoreductase OS=Candidatus Marinimicrobia bacterium PRS2 OX=2483458 GN=EB821_05175 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 9.93VTIKK6 pKa = 9.38YY7 pKa = 7.53TNRR10 pKa = 11.84KK11 pKa = 8.69RR12 pKa = 11.84KK13 pKa = 9.28NKK15 pKa = 10.0HH16 pKa = 4.06GFKK19 pKa = 10.59KK20 pKa = 10.51RR21 pKa = 11.84MSTKK25 pKa = 10.33AGRR28 pKa = 11.84ALLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.79KK37 pKa = 10.3GRR39 pKa = 11.84KK40 pKa = 7.34TISAA44 pKa = 3.98

Molecular weight:
5.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1209

0

1209

424383

35

2954

351.0

39.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.624 ± 0.073

1.382 ± 0.101

6.442 ± 0.1

6.719 ± 0.069

4.701 ± 0.063

7.495 ± 0.097

1.892 ± 0.032

8.633 ± 0.082

5.982 ± 0.113

8.976 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.034

6.087 ± 0.082

3.822 ± 0.035

3.179 ± 0.046

3.414 ± 0.059

7.087 ± 0.068

5.334 ± 0.043

5.649 ± 0.054

1.301 ± 0.03

3.937 ± 0.053

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski