Candidatus Marinimicrobia bacterium PRS2
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1209 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M7KIF0|A0A3M7KIF0_9BACT Uncharacterized protein OS=Candidatus Marinimicrobia bacterium PRS2 OX=2483458 GN=EB821_05875 PE=4 SV=1
SS1 pKa = 6.79 NGGLGGSTVIATVTVNPVNDD21 pKa = 3.86 EE22 pKa = 4.03 PSFEE26 pKa = 4.28 YY27 pKa = 10.92 TNVVAVNEE35 pKa = 4.05 DD36 pKa = 3.32 SGLYY40 pKa = 7.63 EE41 pKa = 4.5 ASWAFNISVGPDD53 pKa = 3.25 NEE55 pKa = 4.47 SDD57 pKa = 3.59 QIPTFEE63 pKa = 5.38 IISNDD68 pKa = 3.2 HH69 pKa = 6.33 EE70 pKa = 5.51 SYY72 pKa = 10.98 FSIQPSISEE81 pKa = 4.23 LGVLTFTPADD91 pKa = 3.8 NQHH94 pKa = 6.04 GEE96 pKa = 4.09 AIITVRR102 pKa = 11.84 LTDD105 pKa = 3.55 NGGTEE110 pKa = 3.97 NGGVNTYY117 pKa = 11.09 DD118 pKa = 3.9 DD119 pKa = 3.41 QTFTIKK125 pKa = 10.63 LNPIADD131 pKa = 4.08 TPNITDD137 pKa = 3.81 AEE139 pKa = 4.16 TDD141 pKa = 3.42 EE142 pKa = 6.81 DD143 pKa = 4.04 IMSSSGLVITRR154 pKa = 11.84 NAVDD158 pKa = 3.77 SEE160 pKa = 4.52 EE161 pKa = 4.12 VTHH164 pKa = 7.03 FKK166 pKa = 9.2 ITGITNGTLYY176 pKa = 10.68 QNNGSTSIANNEE188 pKa = 4.26 FITFTEE194 pKa = 4.74 GNTGLKK200 pKa = 9.9 FDD202 pKa = 5.24 PDD204 pKa = 3.11 EE205 pKa = 5.15 DD206 pKa = 5.24 YY207 pKa = 11.3 YY208 pKa = 11.73 GSGFITIQASVSSSNGGLGGSTVIATVTVNPEE240 pKa = 3.41 NDD242 pKa = 3.5 GPYY245 pKa = 10.26 DD246 pKa = 3.8 AEE248 pKa = 6.0 AGDD251 pKa = 5.2 DD252 pKa = 4.07 IYY254 pKa = 11.41 DD255 pKa = 4.15 ILPSDD260 pKa = 3.82 ATTIDD265 pKa = 3.28 ITILDD270 pKa = 4.11 VSSDD274 pKa = 4.41 DD275 pKa = 3.66 IDD277 pKa = 6.61 DD278 pKa = 4.54 DD279 pKa = 4.82 VSVSHH284 pKa = 6.76 TYY286 pKa = 10.1 SWTLIDD292 pKa = 5.1 ANNGDD297 pKa = 4.61 IIAEE301 pKa = 3.98 ATEE304 pKa = 4.06 INPTFALKK312 pKa = 10.39 AGHH315 pKa = 5.79 YY316 pKa = 8.9 QFEE319 pKa = 4.7 LTVTDD324 pKa = 3.49 TTTNDD329 pKa = 2.94 SNIYY333 pKa = 10.86 GDD335 pKa = 3.52 ITDD338 pKa = 4.11 TDD340 pKa = 4.02 TIDD343 pKa = 3.76 IYY345 pKa = 10.68 IGQPGVDD352 pKa = 3.08 ISSNPVFVTYY362 pKa = 11.05 DD363 pKa = 3.28 DD364 pKa = 3.34 VAYY367 pKa = 9.94 IDD369 pKa = 3.32 IVYY372 pKa = 10.27 FEE374 pKa = 5.05 HH375 pKa = 7.44 EE376 pKa = 4.54 YY377 pKa = 11.46 ASVTQNGDD385 pKa = 3.37 QIVISVPIEE394 pKa = 3.97 YY395 pKa = 10.11 QNKK398 pKa = 7.66 FQFIAGQIIPQDD410 pKa = 3.42 ALEE413 pKa = 4.08 IQIGYY418 pKa = 9.65 SGDD421 pKa = 3.42 VTDD424 pKa = 5.71 ISIEE428 pKa = 4.32 SITTSAITINVVTSHH443 pKa = 6.41 ISEE446 pKa = 3.99 NFYY449 pKa = 10.82 IKK451 pKa = 10.57 LKK453 pKa = 10.19 SVEE456 pKa = 4.19 VNPIDD461 pKa = 3.74 VTSDD465 pKa = 3.41 FSLEE469 pKa = 4.06 IEE471 pKa = 4.22 NDD473 pKa = 3.03 NYY475 pKa = 11.46 LPILKK480 pKa = 9.91 SATEE484 pKa = 3.82 ISIGNPKK491 pKa = 8.31 ITTITDD497 pKa = 3.35 QVLITANPNEE507 pKa = 4.23 KK508 pKa = 9.44 MLDD511 pKa = 3.35 IIVYY515 pKa = 9.56 EE516 pKa = 4.46 DD517 pKa = 3.7 EE518 pKa = 4.34 SVAAINTDD526 pKa = 3.61 DD527 pKa = 5.84 DD528 pKa = 3.95 IVIQFPSTLEE538 pKa = 4.15 NNVKK542 pKa = 9.8 WIQVPTASSNSEE554 pKa = 4.1 KK555 pKa = 10.49 IGTITIDD562 pKa = 3.38 SSDD565 pKa = 3.77 STKK568 pKa = 11.16 LLIDD572 pKa = 4.36 VISDD576 pKa = 3.35 FFAGEE581 pKa = 4.18 SLILTDD587 pKa = 5.28 GYY589 pKa = 11.02 VQVGDD594 pKa = 3.89 IEE596 pKa = 5.24 SNEE599 pKa = 3.71 DD600 pKa = 3.26 DD601 pKa = 4.07 YY602 pKa = 12.12 YY603 pKa = 11.59 KK604 pKa = 10.54 IIAYY608 pKa = 10.21 VNGRR612 pKa = 11.84 NQSLDD617 pKa = 3.61 VEE619 pKa = 4.76 CDD621 pKa = 2.8 NSIIVTSPEE630 pKa = 3.59 LSFASDD636 pKa = 2.57 QHH638 pKa = 6.88 YY639 pKa = 10.64 VVNDD643 pKa = 3.61 SAVSFPTITLTEE655 pKa = 4.1 SSLIPSIYY663 pKa = 10.53 SEE665 pKa = 4.35 VQDD668 pKa = 4.23 SIYY671 pKa = 10.84 LRR673 pKa = 11.84 FDD675 pKa = 3.68 NPCMTWEE682 pKa = 3.84 QVPIEE687 pKa = 4.05 EE688 pKa = 5.28 LNFSGYY694 pKa = 10.85 NGVGDD699 pKa = 4.14 IIYY702 pKa = 10.19 RR703 pKa = 11.84 EE704 pKa = 4.0 NNQTVIINISEE715 pKa = 4.53 PFTISQSLNIDD726 pKa = 3.48 GLKK729 pKa = 10.94 VNICNTNFYY738 pKa = 10.71 NGAGLEE744 pKa = 3.98 YY745 pKa = 10.32 SIRR748 pKa = 11.84 SDD750 pKa = 3.12 PTFYY754 pKa = 11.05 LHH756 pKa = 7.88 DD757 pKa = 4.44 NGEE760 pKa = 4.72 HH761 pKa = 5.87 ICKK764 pKa = 9.91 SSKK767 pKa = 10.44 PIITAIEE774 pKa = 3.96 DD775 pKa = 3.69 EE776 pKa = 4.56 YY777 pKa = 12.0 VNINSISEE785 pKa = 4.46 MITKK789 pKa = 10.24 LIIKK793 pKa = 9.02 EE794 pKa = 4.06 DD795 pKa = 3.61 SVLPIINTTRR805 pKa = 11.84 KK806 pKa = 9.0 IVIWAPDD813 pKa = 3.81 PLIWDD818 pKa = 3.93 SNISLVSLEE827 pKa = 4.37 GQGKK831 pKa = 7.37 TKK833 pKa = 10.46 VSPEE837 pKa = 3.76 LTYY840 pKa = 11.52 SNDD843 pKa = 3.34 NKK845 pKa = 10.32 QLQLDD850 pKa = 3.76 ITDD853 pKa = 3.88 SFSAFDD859 pKa = 3.62 SLVISGMRR867 pKa = 11.84 VKK869 pKa = 10.77 KK870 pKa = 10.48 DD871 pKa = 3.21 GNYY874 pKa = 10.5 SGDD877 pKa = 3.78 FASSAHH883 pKa = 6.0 FKK885 pKa = 10.31 MSLPEE890 pKa = 5.04 SIDD893 pKa = 3.16 SFYY896 pKa = 10.51 PIHH899 pKa = 7.17 LDD901 pKa = 3.3 NSSSDD906 pKa = 3.3 LSIIYY911 pKa = 10.35 SSAHH915 pKa = 6.28 LEE917 pKa = 4.23 FEE919 pKa = 4.47 SSQGANKK926 pKa = 9.92 IVFIKK931 pKa = 10.77 GGANSEE937 pKa = 4.35 YY938 pKa = 10.72 FNVTIFGGSLSNSINIDD955 pKa = 3.16 TDD957 pKa = 3.22 IKK959 pKa = 11.38 LMLPTFLNVHH969 pKa = 4.77 WVEE972 pKa = 4.12 NQSAQIDD979 pKa = 4.32 PGTPAHH985 pKa = 7.32 IINNVASINSLAILPNTNNKK1005 pKa = 8.5 VLNLNVLEE1013 pKa = 4.63 AFSINDD1019 pKa = 3.63 SIKK1022 pKa = 10.37 INNLSVEE1029 pKa = 4.27 NNTTVNDD1036 pKa = 3.71 MSKK1039 pKa = 9.94 IDD1041 pKa = 4.14 YY1042 pKa = 10.73 LSLLQGDD1049 pKa = 4.23 YY1050 pKa = 11.24 GSNNHH1055 pKa = 7.43 AINQADD1061 pKa = 4.15 SLIVAAPQMMIAKK1074 pKa = 9.56 DD1075 pKa = 3.14 ITVITGALEE1084 pKa = 4.51 NINIPPITIVDD1095 pKa = 3.75 DD1096 pKa = 3.78 TLEE1099 pKa = 3.93 QVLNIGNNNFYY1110 pKa = 10.69 YY1111 pKa = 10.78 LSLEE1115 pKa = 4.58 GISNYY1120 pKa = 9.73 EE1121 pKa = 3.57 WDD1123 pKa = 4.75 SGFTCLNMLCTLSDD1137 pKa = 3.11 NKK1139 pKa = 10.07 TIKK1142 pKa = 10.45 FLIQSSGTLTISNLKK1157 pKa = 9.61 LKK1159 pKa = 10.71 NVTFDD1164 pKa = 3.61 NTDD1167 pKa = 3.11 DD1168 pKa = 4.01 AYY1170 pKa = 11.43 RR1171 pKa = 11.84 LVLKK1175 pKa = 10.65 DD1176 pKa = 4.17 NNTKK1180 pKa = 10.08 MIAKK1184 pKa = 9.69 SINTIKK1190 pKa = 10.04 TGMPKK1195 pKa = 9.13 IDD1197 pKa = 5.8 LITGVIWVDD1206 pKa = 3.39 LDD1208 pKa = 4.0 SIKK1211 pKa = 10.82 AISTLKK1217 pKa = 10.19 IIEE1220 pKa = 4.74 SINHH1224 pKa = 6.13 ISSDD1228 pKa = 3.7 SLKK1231 pKa = 10.91 LYY1233 pKa = 10.4 FDD1235 pKa = 3.57 KK1236 pKa = 11.11 SYY1238 pKa = 11.27 LKK1240 pKa = 9.84 WDD1242 pKa = 3.69 QQSSVQSTINYY1253 pKa = 7.23 GFPSEE1258 pKa = 4.28 GEE1260 pKa = 4.09 LYY1262 pKa = 10.41 INQYY1266 pKa = 11.0 EE1267 pKa = 4.23 NGSNSAFDD1275 pKa = 4.27 LSAFNFLITSDD1286 pKa = 3.67 VFKK1289 pKa = 10.78 DD1290 pKa = 2.97 ITQADD1295 pKa = 3.57 TVMNLNCVLMDD1306 pKa = 4.34 EE1307 pKa = 4.78 YY1308 pKa = 11.67 NQFTITHH1315 pKa = 6.9 PIILNFDD1322 pKa = 3.99 PLISDD1327 pKa = 4.41 VIPLGGTTWDD1337 pKa = 3.65 SLTLSVIINEE1347 pKa = 4.21 NFLEE1351 pKa = 5.14 DD1352 pKa = 3.83 EE1353 pKa = 4.44 GTDD1356 pKa = 3.53 LSSIRR1361 pKa = 11.84 PTLLLLDD1368 pKa = 4.49 GEE1370 pKa = 5.35 GDD1372 pKa = 3.48 TSNYY1376 pKa = 10.35 DD1377 pKa = 2.86 IDD1379 pKa = 4.25 VSFTQTAAQGDD1390 pKa = 4.44 FPEE1393 pKa = 4.68 GVYY1396 pKa = 9.07 TAEE1399 pKa = 4.83 FYY1401 pKa = 11.06 FPDD1404 pKa = 3.7 SLIDD1408 pKa = 3.46 ILTSKK1413 pKa = 10.68 QEE1415 pKa = 4.01 NNDD1418 pKa = 2.88 IDD1420 pKa = 4.98 LLLSLTDD1427 pKa = 3.36 IDD1429 pKa = 4.82 SNALSRR1435 pKa = 11.84 SNSSKK1440 pKa = 11.15 DD1441 pKa = 3.12 NWKK1444 pKa = 8.89 LTPISLGWSEE1454 pKa = 5.87 DD1455 pKa = 2.91 ITNIYY1460 pKa = 10.01 LDD1462 pKa = 3.16 QSLRR1466 pKa = 11.84 YY1467 pKa = 9.75 LNPDD1471 pKa = 3.03 SNDD1474 pKa = 2.95 IVFTLPEE1481 pKa = 5.16 PISTTCEE1488 pKa = 3.23 IRR1490 pKa = 11.84 INNLVSDD1497 pKa = 4.07 STYY1500 pKa = 11.11 EE1501 pKa = 4.0 LGTMAINEE1509 pKa = 4.15 NSTITIEE1516 pKa = 4.13 GTQIQNALGRR1526 pKa = 11.84 DD1527 pKa = 3.09 ADD1529 pKa = 4.41 GIYY1532 pKa = 10.34 DD1533 pKa = 5.27 IILQDD1538 pKa = 3.3 KK1539 pKa = 10.53 SGGSFSFPFIKK1550 pKa = 10.25 RR1551 pKa = 11.84 IIIDD1555 pKa = 3.7 TSPPQFISINPTTDD1569 pKa = 2.9 VFDD1572 pKa = 4.36 SGWNEE1577 pKa = 3.81 NSVHH1581 pKa = 7.11 SITDD1585 pKa = 2.99 QDD1587 pKa = 4.63 KK1588 pKa = 11.0 IILTFKK1594 pKa = 10.14 DD1595 pKa = 4.09 YY1596 pKa = 11.03 PIMTTVDD1603 pKa = 3.58 SIFIYY1608 pKa = 10.78 NSGIVSAMAPGGYY1621 pKa = 9.88 LFNDD1625 pKa = 4.28 SIDD1628 pKa = 3.47 IQISFEE1634 pKa = 4.16 LTDD1637 pKa = 3.66 SNGNDD1642 pKa = 3.11 TSYY1645 pKa = 10.81 TFLNTDD1651 pKa = 3.37 EE1652 pKa = 5.41 NGLLDD1657 pKa = 4.44 FYY1659 pKa = 11.19 KK1660 pKa = 10.75 EE1661 pKa = 4.31 SIIKK1665 pKa = 10.68 LSDD1668 pKa = 3.22 HH1669 pKa = 5.28 QLEE1672 pKa = 4.63 GKK1674 pKa = 10.17 NSIIINYY1681 pKa = 8.55 VLSDD1685 pKa = 3.47 HH1686 pKa = 7.26 AGNEE1690 pKa = 3.75 QNYY1693 pKa = 7.55 TIKK1696 pKa = 10.8 YY1697 pKa = 8.05 VLYY1700 pKa = 9.97 NASDD1704 pKa = 3.4 IQGLVDD1710 pKa = 6.26 DD1711 pKa = 5.25 IFNFPNPFSSVDD1723 pKa = 2.79 EE1724 pKa = 4.37 GTRR1727 pKa = 11.84 IRR1729 pKa = 11.84 YY1730 pKa = 9.47 SLTQDD1735 pKa = 3.23 GNQGKK1740 pKa = 9.3 YY1741 pKa = 7.46 VVFNAKK1747 pKa = 10.4 GEE1749 pKa = 4.25 LVYY1752 pKa = 10.41 HH1753 pKa = 6.19 YY1754 pKa = 10.03 SLSSSEE1760 pKa = 4.19 LQQGTHH1766 pKa = 6.09 TIYY1769 pKa = 10.47 WDD1771 pKa = 3.28 GHH1773 pKa = 4.78 YY1774 pKa = 11.08 LNNNYY1779 pKa = 10.28 LLATGVYY1786 pKa = 10.21 FGFLDD1791 pKa = 4.37 IDD1793 pKa = 4.11 DD1794 pKa = 5.66 KK1795 pKa = 11.12 IAKK1798 pKa = 9.63 HH1799 pKa = 6.23 KK1800 pKa = 10.38 IAIINN1805 pKa = 3.58
Molecular weight: 199.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.846
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.24
Thurlkill 3.706
EMBOSS 3.808
Sillero 4.012
Patrickios 1.608
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A3M7KIZ1|A0A3M7KIZ1_9BACT Electron transfer flavoprotein-ubiquinone oxidoreductase OS=Candidatus Marinimicrobia bacterium PRS2 OX=2483458 GN=EB821_05175 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 9.93 VTIKK6 pKa = 9.38 YY7 pKa = 7.53 TNRR10 pKa = 11.84 KK11 pKa = 8.69 RR12 pKa = 11.84 KK13 pKa = 9.28 NKK15 pKa = 10.0 HH16 pKa = 4.06 GFKK19 pKa = 10.59 KK20 pKa = 10.51 RR21 pKa = 11.84 MSTKK25 pKa = 10.33 AGRR28 pKa = 11.84 ALLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.79 KK37 pKa = 10.3 GRR39 pKa = 11.84 KK40 pKa = 7.34 TISAA44 pKa = 3.98
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 11.023
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.427
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.149
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.956
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1209
0
1209
424383
35
2954
351.0
39.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.624 ± 0.073
1.382 ± 0.101
6.442 ± 0.1
6.719 ± 0.069
4.701 ± 0.063
7.495 ± 0.097
1.892 ± 0.032
8.633 ± 0.082
5.982 ± 0.113
8.976 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.344 ± 0.034
6.087 ± 0.082
3.822 ± 0.035
3.179 ± 0.046
3.414 ± 0.059
7.087 ± 0.068
5.334 ± 0.043
5.649 ± 0.054
1.301 ± 0.03
3.937 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here