Moranella endobia (strain PCIT)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 405 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F7XY41|F7XY41_MOREP 50S ribosomal protein L23 OS=Moranella endobia (strain PCIT) OX=903503 GN=rplW PE=3 SV=1
MM1 pKa = 7.8 AYY3 pKa = 9.56 ISIVFGSDD11 pKa = 2.48 TGNTEE16 pKa = 4.43 NIAKK20 pKa = 9.05 MLQQKK25 pKa = 10.43 LGTEE29 pKa = 4.21 VADD32 pKa = 4.86 LFDD35 pKa = 4.05 IANSSEE41 pKa = 4.17 KK42 pKa = 10.66 DD43 pKa = 3.19 LEE45 pKa = 4.3 QYY47 pKa = 11.0 DD48 pKa = 4.21 RR49 pKa = 11.84 LLLGIPTWYY58 pKa = 10.37 YY59 pKa = 11.64 GEE61 pKa = 4.69 AQCDD65 pKa = 3.21 WDD67 pKa = 4.63 DD68 pKa = 3.82 FFPRR72 pKa = 11.84 LEE74 pKa = 4.71 NIDD77 pKa = 4.32 FSSKK81 pKa = 8.31 MVAIFGCGDD90 pKa = 3.25 QEE92 pKa = 5.04 DD93 pKa = 4.3 YY94 pKa = 11.79 AEE96 pKa = 4.32 YY97 pKa = 10.5 FCDD100 pKa = 4.7 AMGTLRR106 pKa = 11.84 DD107 pKa = 3.63 IIEE110 pKa = 4.44 PKK112 pKa = 8.87 GATMVGHH119 pKa = 7.28 WPTSGYY125 pKa = 10.2 HH126 pKa = 4.64 FHH128 pKa = 7.25 ASKK131 pKa = 11.13 GMVDD135 pKa = 3.64 DD136 pKa = 3.86 AHH138 pKa = 6.9 FIGLAIDD145 pKa = 4.1 EE146 pKa = 4.62 DD147 pKa = 4.24 RR148 pKa = 11.84 QPEE151 pKa = 4.35 LTSEE155 pKa = 5.13 RR156 pKa = 11.84 VDD158 pKa = 2.95 NWINQVRR165 pKa = 11.84 VEE167 pKa = 4.23 MNLSS171 pKa = 3.21
Molecular weight: 19.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.139
IPC2_protein 4.151
IPC_protein 4.126
Toseland 3.923
ProMoST 4.266
Dawson 4.101
Bjellqvist 4.253
Wikipedia 4.012
Rodwell 3.948
Grimsley 3.834
Solomon 4.101
Lehninger 4.05
Nozaki 4.215
DTASelect 4.431
Thurlkill 3.961
EMBOSS 4.024
Sillero 4.24
Patrickios 3.21
IPC_peptide 4.088
IPC2_peptide 4.228
IPC2.peptide.svr19 4.152
Protein with the highest isoelectric point:
>tr|F7XX64|F7XX64_MOREP Probable GTP-binding protein EngB OS=Moranella endobia (strain PCIT) OX=903503 GN=engB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.53 RR3 pKa = 11.84 TFQPSILKK11 pKa = 10.36 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTNNGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 VKK37 pKa = 10.57 GRR39 pKa = 11.84 IRR41 pKa = 11.84 LTVSTKK47 pKa = 8.66 ITT49 pKa = 3.33
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
405
0
405
137782
33
1402
340.2
37.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.428 ± 0.112
1.287 ± 0.037
4.947 ± 0.079
5.58 ± 0.112
3.499 ± 0.075
6.937 ± 0.111
2.483 ± 0.055
7.266 ± 0.088
5.067 ± 0.094
10.647 ± 0.146
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.78 ± 0.05
4.516 ± 0.076
3.805 ± 0.061
4.44 ± 0.075
5.925 ± 0.094
5.695 ± 0.083
5.489 ± 0.067
7.081 ± 0.106
1.057 ± 0.048
3.073 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here