Rhodococcus phage ReqiPine5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4P837|D4P837_9CAUD Gp62 OS=Rhodococcus phage ReqiPine5 OX=691963 GN=ReqiPine5gene62 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84PHH4 pKa = 6.86EE5 pKa = 4.39ACSRR9 pKa = 11.84CGAVGPVEE17 pKa = 3.84QSFISLDD24 pKa = 3.68WMCDD28 pKa = 3.12DD29 pKa = 4.92CLEE32 pKa = 4.84DD33 pKa = 3.5EE34 pKa = 4.61

Molecular weight: 3.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4P859|D4P859_9CAUD Gp84 OS=Rhodococcus phage ReqiPine5 OX=691963 GN=ReqiPine5gene84 PE=4 SV=1
MM1 pKa = 7.45ISSFFSGIGYY11 pKa = 9.76LIGIVLGVGMIAVGLVIVVGVLGWMLRR38 pKa = 11.84TGLRR42 pKa = 11.84FAIDD46 pKa = 3.52VFSGFVPGLRR56 pKa = 11.84RR57 pKa = 11.84WAKK60 pKa = 10.75RR61 pKa = 11.84NDD63 pKa = 3.28QTTDD67 pKa = 2.91LL68 pKa = 4.43

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

18237

30

1732

217.1

23.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.208 ± 0.346

0.877 ± 0.122

6.717 ± 0.229

5.928 ± 0.299

2.994 ± 0.174

8.669 ± 0.416

1.958 ± 0.169

4.984 ± 0.234

3.378 ± 0.266

7.896 ± 0.169

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.122

2.731 ± 0.179

5.692 ± 0.305

2.797 ± 0.137

6.443 ± 0.394

5.275 ± 0.176

7.342 ± 0.207

8.39 ± 0.305

1.963 ± 0.108

2.473 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski