Bacillus sp. TS-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X0RAK5|X0RAK5_9BACI Putative alcohol dehydrogenase OS=Bacillus sp. TS-2 OX=1450694 GN=BTS2_2949 PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.54KK3 pKa = 10.18MMMACFSICLLFIVVACSSGNSDD26 pKa = 3.28TTSNEE31 pKa = 3.34GSTGDD36 pKa = 4.76QEE38 pKa = 5.89DD39 pKa = 4.5EE40 pKa = 4.3NQSSSVDD47 pKa = 4.14EE48 pKa = 3.94ITIWAWDD55 pKa = 3.79PNFNIAALEE64 pKa = 4.05LAKK67 pKa = 9.97DD68 pKa = 4.05TYY70 pKa = 11.47AEE72 pKa = 4.05EE73 pKa = 4.2NADD76 pKa = 3.47VNINIVEE83 pKa = 4.29SAQDD87 pKa = 4.67DD88 pKa = 4.07IIQRR92 pKa = 11.84LNTILSSGSTSGLPHH107 pKa = 6.48IVLIEE112 pKa = 4.29DD113 pKa = 3.33YY114 pKa = 10.05RR115 pKa = 11.84AQGFLQSYY123 pKa = 8.73PDD125 pKa = 3.83SFHH128 pKa = 7.41EE129 pKa = 3.81ITGAYY134 pKa = 9.42EE135 pKa = 4.32LDD137 pKa = 3.2NFAPYY142 pKa = 10.07KK143 pKa = 10.01IAPTSIDD150 pKa = 3.42DD151 pKa = 4.04ANYY154 pKa = 10.68GLPFDD159 pKa = 4.04TGVAGLYY166 pKa = 10.3VRR168 pKa = 11.84TDD170 pKa = 3.44YY171 pKa = 11.31FEE173 pKa = 4.04EE174 pKa = 5.47AGYY177 pKa = 10.45SVEE180 pKa = 5.39DD181 pKa = 4.13LQSLDD186 pKa = 3.07WNEE189 pKa = 4.17FIEE192 pKa = 4.21IGKK195 pKa = 9.59KK196 pKa = 9.7IKK198 pKa = 10.21EE199 pKa = 4.1ATGKK203 pKa = 10.46YY204 pKa = 10.46LLTLEE209 pKa = 4.83PNNLSQLRR217 pKa = 11.84MMIQTNGGWYY227 pKa = 9.79VEE229 pKa = 4.3EE230 pKa = 5.84DD231 pKa = 3.69GVTPYY236 pKa = 11.0LADD239 pKa = 4.01NEE241 pKa = 4.18DD242 pKa = 3.44LKK244 pKa = 11.13IAFEE248 pKa = 4.39VYY250 pKa = 8.43KK251 pKa = 10.28TLIDD255 pKa = 3.74EE256 pKa = 5.29DD257 pKa = 4.13LAKK260 pKa = 10.47PISDD264 pKa = 3.12WSQFVAGFNSGEE276 pKa = 4.28VASVPTGNWITPSVTAEE293 pKa = 3.94EE294 pKa = 4.46SQSGDD299 pKa = 2.87WAVVPFPTLPGVEE312 pKa = 4.18SSIPATNLGGSSWYY326 pKa = 10.17VLNIDD331 pKa = 4.07GKK333 pKa = 11.04EE334 pKa = 3.93DD335 pKa = 3.46ATDD338 pKa = 4.32FLAKK342 pKa = 9.98TFGSNDD348 pKa = 3.23QLYY351 pKa = 10.44EE352 pKa = 4.24DD353 pKa = 4.95LLNEE357 pKa = 3.94IGAIGTYY364 pKa = 10.46LPASANEE371 pKa = 4.24AYY373 pKa = 10.16QSEE376 pKa = 4.83VEE378 pKa = 4.24FFGNQSIYY386 pKa = 11.27ADD388 pKa = 3.56FAKK391 pKa = 9.94WSEE394 pKa = 4.91LIPQVNYY401 pKa = 10.76GIHH404 pKa = 6.26TYY406 pKa = 10.28SIEE409 pKa = 5.05DD410 pKa = 3.38ILVVEE415 pKa = 4.28MQNYY419 pKa = 10.28LNGKK423 pKa = 9.82DD424 pKa = 3.56IDD426 pKa = 3.95QALQDD431 pKa = 3.83AQVQAEE437 pKa = 4.27TQIRR441 pKa = 3.63

Molecular weight: 48.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X0SRG3|X0SRG3_9BACI Putatice ATPase OS=Bacillus sp. TS-2 OX=1450694 GN=BTS2_3321 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4084

0

4084

1205149

39

3910

295.1

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.154 ± 0.036

0.636 ± 0.01

5.06 ± 0.028

8.138 ± 0.044

4.698 ± 0.033

6.334 ± 0.039

2.226 ± 0.019

8.172 ± 0.032

6.723 ± 0.047

10.009 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.02

4.745 ± 0.033

3.419 ± 0.022

4.149 ± 0.027

3.812 ± 0.025

6.46 ± 0.029

5.256 ± 0.027

6.565 ± 0.029

1.088 ± 0.015

3.665 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski