candidate division MSBL1 archaeon SCGC-AAA382K21

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VKM0|A0A133VKM0_9EURY Phosphohydrolase OS=candidate division MSBL1 archaeon SCGC-AAA382K21 OX=1698283 GN=AKJ54_00890 PE=4 SV=1
MM1 pKa = 7.34TWDD4 pKa = 3.31QGNYY8 pKa = 9.41IYY10 pKa = 10.51TLFGGSYY17 pKa = 10.32SDD19 pKa = 3.39TSGDD23 pKa = 3.26SRR25 pKa = 11.84HH26 pKa = 5.53YY27 pKa = 9.55FWRR30 pKa = 11.84YY31 pKa = 10.18DD32 pKa = 3.24ISSDD36 pKa = 3.33SWEE39 pKa = 4.02QLEE42 pKa = 4.5NTPSGQGAGDD52 pKa = 5.16AITWVPGSAVGTSNDD67 pKa = 2.77WVYY70 pKa = 11.33AIVGSKK76 pKa = 10.32VSEE79 pKa = 4.07HH80 pKa = 6.62GIAFYY85 pKa = 10.45RR86 pKa = 11.84YY87 pKa = 10.1NINTNNWQTLQYY99 pKa = 9.11NPSWATEE106 pKa = 3.96GSDD109 pKa = 4.77DD110 pKa = 4.63GSSLVWAGGDD120 pKa = 3.48YY121 pKa = 10.66LYY123 pKa = 11.2SLQGEE128 pKa = 4.44HH129 pKa = 7.07AEE131 pKa = 4.7SDD133 pKa = 3.87PADD136 pKa = 3.64DD137 pKa = 3.71RR138 pKa = 11.84AFARR142 pKa = 11.84FDD144 pKa = 3.13ITDD147 pKa = 3.74NSWTDD152 pKa = 3.25LDD154 pKa = 5.57DD155 pKa = 4.01IPEE158 pKa = 4.3EE159 pKa = 4.47GGVGDD164 pKa = 4.43GGSLAWAGGNYY175 pKa = 9.37SNKK178 pKa = 9.46VYY180 pKa = 10.9ALGGGFVGRR189 pKa = 11.84GEE191 pKa = 4.27TPGDD195 pKa = 3.15NFYY198 pKa = 11.4VYY200 pKa = 9.17DD201 pKa = 3.89TSVDD205 pKa = 2.81NWTQLQSLPYY215 pKa = 10.89GMTGQNGPRR224 pKa = 11.84LGYY227 pKa = 10.8ADD229 pKa = 3.67GSLYY233 pKa = 10.4CWRR236 pKa = 11.84GYY238 pKa = 11.12YY239 pKa = 9.86DD240 pKa = 3.97ASNNPDD246 pKa = 2.84VLMAYY251 pKa = 7.54EE252 pKa = 4.53LPAVGVSVSISPSEE266 pKa = 3.88NNALNGEE273 pKa = 4.34NVTFTVTVTNVDD285 pKa = 3.98DD286 pKa = 3.74EE287 pKa = 4.48HH288 pKa = 8.42HH289 pKa = 6.6NFVLEE294 pKa = 4.27NSDD297 pKa = 3.69SLDD300 pKa = 3.21WQMSLSEE307 pKa = 5.35DD308 pKa = 3.91NLSLDD313 pKa = 3.97PDD315 pKa = 3.41NSEE318 pKa = 4.04NVVLEE323 pKa = 4.03VAIPPAVSGGTEE335 pKa = 3.93DD336 pKa = 3.8TVTVTATAQDD346 pKa = 3.49NAEE349 pKa = 4.13ISSSAGCTAGAQVDD363 pKa = 3.45VDD365 pKa = 4.1FNVWITPFSQTGSRR379 pKa = 11.84EE380 pKa = 3.96DD381 pKa = 3.35NLDD384 pKa = 3.54YY385 pKa = 11.0VVHH388 pKa = 6.27VKK390 pKa = 10.85NSGNVVDD397 pKa = 5.59NYY399 pKa = 11.7DD400 pKa = 5.98LIITDD405 pKa = 3.56NAGWADD411 pKa = 3.78NVSLAGSGYY420 pKa = 10.66SDD422 pKa = 4.52ADD424 pKa = 3.86NIFPSADD431 pKa = 3.16AGICEE436 pKa = 4.69YY437 pKa = 11.14YY438 pKa = 10.34PNTTKK443 pKa = 10.99GGDD446 pKa = 3.3NYY448 pKa = 11.02NVYY451 pKa = 10.41VGYY454 pKa = 10.52EE455 pKa = 3.91NNPYY459 pKa = 10.29KK460 pKa = 10.38PNCTGEE466 pKa = 3.98DD467 pKa = 3.15TRR469 pKa = 11.84ALFKK473 pKa = 10.67FDD475 pKa = 4.8LSGIPDD481 pKa = 4.13GATITGATLNAHH493 pKa = 6.75GDD495 pKa = 4.09FSPSTGFPNYY505 pKa = 9.6NDD507 pKa = 4.89RR508 pKa = 11.84IMLTDD513 pKa = 3.65VQPLGSDD520 pKa = 2.6SWGEE524 pKa = 3.81NTVNWNNKK532 pKa = 8.55PSFGSTLDD540 pKa = 3.49SVLIDD545 pKa = 4.66DD546 pKa = 4.67NEE548 pKa = 4.06YY549 pKa = 10.34WSWNVKK555 pKa = 10.11SAVQNEE561 pKa = 4.09VSGDD565 pKa = 3.75GVVSLGLLSEE575 pKa = 4.36NAVSNQKK582 pKa = 10.13NVTMWFHH589 pKa = 6.28TKK591 pKa = 10.32DD592 pKa = 3.69GSPEE596 pKa = 3.93NPDD599 pKa = 3.14SVKK602 pKa = 10.47PYY604 pKa = 11.02LEE606 pKa = 4.03VLYY609 pKa = 10.15EE610 pKa = 4.18VYY612 pKa = 10.74DD613 pKa = 3.91VVSLKK618 pKa = 10.84NISPGEE624 pKa = 4.06NEE626 pKa = 3.84NVALNVTIPNSAASGDD642 pKa = 3.77HH643 pKa = 4.73TVKK646 pKa = 10.85VGATSYY652 pKa = 11.78GDD654 pKa = 3.8DD655 pKa = 3.95SLSKK659 pKa = 10.46EE660 pKa = 4.22NSCIASVGSPTGVGVSISPSEE681 pKa = 4.58DD682 pKa = 3.18NAPPGYY688 pKa = 9.89GLNYY692 pKa = 9.84EE693 pKa = 4.33VTVTNTGSISDD704 pKa = 4.19NYY706 pKa = 10.62DD707 pKa = 3.13LSVVDD712 pKa = 4.1NAVPSWNPTLDD723 pKa = 3.14NSLFEE728 pKa = 4.75DD729 pKa = 4.07VPPGEE734 pKa = 4.19NRR736 pKa = 11.84NTVLRR741 pKa = 11.84VTVPEE746 pKa = 3.91NANLGVEE753 pKa = 4.28DD754 pKa = 4.82NIIVTASSQEE764 pKa = 4.02NVEE767 pKa = 3.93MDD769 pKa = 4.53DD770 pKa = 3.76NASCIALSAALFRR783 pKa = 11.84SVKK786 pKa = 10.11ISVSPSYY793 pKa = 10.62KK794 pKa = 10.11AGSSGSSLDD803 pKa = 3.49YY804 pKa = 10.67SVKK807 pKa = 10.26VKK809 pKa = 10.72NRR811 pKa = 11.84GNIPDD816 pKa = 3.88NYY818 pKa = 9.85EE819 pKa = 4.04LEE821 pKa = 4.65ASDD824 pKa = 5.82DD825 pKa = 4.21VFPSWDD831 pKa = 3.45PTVVDD836 pKa = 4.58NIFLNVVPEE845 pKa = 4.17EE846 pKa = 4.16EE847 pKa = 4.38RR848 pKa = 11.84VTTLNVVIPEE858 pKa = 3.96NDD860 pKa = 3.22NLRR863 pKa = 11.84GIEE866 pKa = 4.08DD867 pKa = 4.47NITTTVTSLEE877 pKa = 4.11NDD879 pKa = 3.78SVHH882 pKa = 7.54DD883 pKa = 4.05SAVCTAQAMDD893 pKa = 5.02SISMQLDD900 pKa = 4.07LVADD904 pKa = 4.13WNLVGLPVVSDD915 pKa = 3.49STTKK919 pKa = 10.58EE920 pKa = 3.69NLFEE924 pKa = 4.3GTGVDD929 pKa = 3.75PLSVV933 pKa = 3.14

Molecular weight: 100.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VKJ0|A0A133VKJ0_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382K21 OX=1698283 GN=AKJ54_00950 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.04SDD4 pKa = 3.61KK5 pKa = 11.11NKK7 pKa = 10.4GPSITSGKK15 pKa = 8.72TSFRR19 pKa = 11.84SLTGPIHH26 pKa = 7.01SIRR29 pKa = 11.84NLGQLGKK36 pKa = 10.45VRR38 pKa = 11.84TDD40 pKa = 2.82RR41 pKa = 11.84ATLSKK46 pKa = 10.55RR47 pKa = 11.84IFQEE51 pKa = 3.98EE52 pKa = 4.56VVPRR56 pKa = 11.84RR57 pKa = 11.84VGGLAVKK64 pKa = 10.67AFGDD68 pKa = 3.79LRR70 pKa = 11.84LDD72 pKa = 3.82SPQDD76 pKa = 3.35TKK78 pKa = 11.17KK79 pKa = 10.74YY80 pKa = 8.15SQEE83 pKa = 3.49RR84 pKa = 11.84GKK86 pKa = 10.83RR87 pKa = 11.84IIEE90 pKa = 4.38DD91 pKa = 3.48NSRR94 pKa = 11.84LDD96 pKa = 3.44PVFRR100 pKa = 11.84PVGG103 pKa = 3.36

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

218

0

218

61194

53

1467

280.7

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.852 ± 0.155

0.833 ± 0.045

6.102 ± 0.148

9.754 ± 0.273

3.798 ± 0.125

7.517 ± 0.153

1.646 ± 0.066

7.002 ± 0.165

7.377 ± 0.278

9.117 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.08

4.492 ± 0.188

3.852 ± 0.098

2.229 ± 0.07

5.396 ± 0.169

7.12 ± 0.208

4.804 ± 0.15

6.912 ± 0.184

1.159 ± 0.067

2.976 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski