Salmonella phage BP63
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140XG45|A0A140XG45_9CAUD Uncharacterized protein OS=Salmonella phage BP63 OX=1543205 GN=BP63_14 PE=4 SV=1
MM1 pKa = 7.75 KK2 pKa = 10.36 DD3 pKa = 4.96 NYY5 pKa = 8.64 TLWYY9 pKa = 9.75 DD10 pKa = 3.63 CNEE13 pKa = 4.18 GLWCVNINNGPVPDD27 pKa = 4.48 FEE29 pKa = 5.47 SMDD32 pKa = 3.62 VEE34 pKa = 4.45 DD35 pKa = 5.59 CVDD38 pKa = 4.94 FIQSQVVKK46 pKa = 10.64 YY47 pKa = 10.22 QGG49 pKa = 2.92
Molecular weight: 5.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.925
IPC2_protein 3.656
IPC_protein 3.516
Toseland 3.338
ProMoST 3.757
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.554
Rodwell 3.376
Grimsley 3.261
Solomon 3.49
Lehninger 3.439
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.439
EMBOSS 3.554
Sillero 3.656
Patrickios 0.006
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.674
Protein with the highest isoelectric point:
>tr|A0A140XG57|A0A140XG57_9CAUD Uncharacterized protein OS=Salmonella phage BP63 OX=1543205 GN=BP63_26 PE=4 SV=1
MM1 pKa = 7.46 IRR3 pKa = 11.84 IRR5 pKa = 11.84 LEE7 pKa = 3.74 RR8 pKa = 11.84 RR9 pKa = 11.84 DD10 pKa = 3.51 KK11 pKa = 11.14 QKK13 pKa = 11.29 FEE15 pKa = 3.54 FTYY18 pKa = 10.47 RR19 pKa = 11.84 AFKK22 pKa = 10.7 SFEE25 pKa = 4.05 GQGVSVGVHH34 pKa = 6.16 PEE36 pKa = 3.42 HH37 pKa = 6.82 NRR39 pKa = 11.84 RR40 pKa = 11.84 INPRR44 pKa = 11.84 DD45 pKa = 3.53 RR46 pKa = 11.84 ITNAEE51 pKa = 3.92 LAMVHH56 pKa = 6.6 EE57 pKa = 4.93 YY58 pKa = 11.07 GLNGLPEE65 pKa = 4.16 RR66 pKa = 11.84 SFLRR70 pKa = 11.84 SAVGGRR76 pKa = 11.84 GKK78 pKa = 10.56 GRR80 pKa = 11.84 QAINKK85 pKa = 8.43 AFRR88 pKa = 11.84 DD89 pKa = 3.76 NVPAVLRR96 pKa = 11.84 GSMTAHH102 pKa = 7.02 EE103 pKa = 5.17 LNDD106 pKa = 3.9 KK107 pKa = 9.75 IGRR110 pKa = 11.84 MLVDD114 pKa = 4.0 AVHH117 pKa = 7.13 DD118 pKa = 4.29 RR119 pKa = 11.84 MDD121 pKa = 4.21 SDD123 pKa = 4.09 VPPPNTEE130 pKa = 3.51 LTEE133 pKa = 3.94 EE134 pKa = 4.36 RR135 pKa = 11.84 KK136 pKa = 10.02 RR137 pKa = 11.84 GPGTLRR143 pKa = 11.84 EE144 pKa = 4.05 SMQLYY149 pKa = 10.63 DD150 pKa = 3.76 SIGYY154 pKa = 9.31 KK155 pKa = 9.94 VGRR158 pKa = 11.84 NWRR161 pKa = 11.84 LL162 pKa = 2.91
Molecular weight: 18.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.443
IPC_protein 10.028
Toseland 10.438
ProMoST 10.101
Dawson 10.555
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 10.76
Grimsley 10.613
Solomon 10.643
Lehninger 10.613
Nozaki 10.409
DTASelect 10.248
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.482
Patrickios 10.482
IPC_peptide 10.643
IPC2_peptide 8.873
IPC2.peptide.svr19 8.814
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
0
76
16248
37
985
213.8
23.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.303 ± 0.295
1.219 ± 0.11
6.425 ± 0.183
6.345 ± 0.281
3.422 ± 0.171
6.979 ± 0.238
1.68 ± 0.149
5.915 ± 0.181
6.112 ± 0.31
7.859 ± 0.256
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.8 ± 0.153
5.238 ± 0.206
4.228 ± 0.222
3.748 ± 0.2
4.641 ± 0.221
5.755 ± 0.279
6.419 ± 0.355
7.515 ± 0.36
1.662 ± 0.145
3.736 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here