Avian-like circovirus
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A172MHN5|A0A172MHN5_9CIRC ATP-dependent helicase Rep OS=Avian-like circovirus OX=1844928 PE=3 SV=1
MM1 pKa = 7.47 PPVRR5 pKa = 11.84 AAPAKK10 pKa = 9.69 RR11 pKa = 11.84 WCFTLNNPTDD21 pKa = 3.72 EE22 pKa = 4.39 EE23 pKa = 4.38 VQKK26 pKa = 10.87 IKK28 pKa = 11.16 DD29 pKa = 3.82 MNPSEE34 pKa = 4.01 YY35 pKa = 10.16 HH36 pKa = 4.82 YY37 pKa = 10.93 AIVGKK42 pKa = 10.07 EE43 pKa = 3.83 RR44 pKa = 11.84 GEE46 pKa = 3.88 QGTPHH51 pKa = 6.3 LQGFLHH57 pKa = 6.5 LKK59 pKa = 9.23 CKK61 pKa = 10.31 KK62 pKa = 10.1 RR63 pKa = 11.84 LNQLKK68 pKa = 9.89 EE69 pKa = 4.03 FLARR73 pKa = 11.84 AHH75 pKa = 5.97 WEE77 pKa = 3.8 KK78 pKa = 11.15 ARR80 pKa = 11.84 GSDD83 pKa = 3.67 EE84 pKa = 5.44 DD85 pKa = 3.88 NEE87 pKa = 4.7 AYY89 pKa = 10.06 CSKK92 pKa = 10.06 EE93 pKa = 3.77 GEE95 pKa = 4.45 VILTLGMPAKK105 pKa = 10.49 GNRR108 pKa = 11.84 SDD110 pKa = 4.64 LSGAVAAVKK119 pKa = 10.29 AGRR122 pKa = 11.84 AMSEE126 pKa = 3.74 IARR129 pKa = 11.84 EE130 pKa = 3.83 FSEE133 pKa = 5.75 VYY135 pKa = 10.0 VKK137 pKa = 10.63 YY138 pKa = 10.91 GRR140 pKa = 11.84 GLRR143 pKa = 11.84 DD144 pKa = 3.22 LALLIGQKK152 pKa = 10.21 PRR154 pKa = 11.84 DD155 pKa = 3.85 FKK157 pKa = 11.14 TDD159 pKa = 3.4 VILITGPSGVGKK171 pKa = 10.22 SRR173 pKa = 11.84 WAFEE177 pKa = 4.16 YY178 pKa = 10.24 PGSKK182 pKa = 9.65 FYY184 pKa = 11.33 KK185 pKa = 9.9 MKK187 pKa = 10.93 GEE189 pKa = 3.76 WWDD192 pKa = 3.97 GYY194 pKa = 11.17 ANEE197 pKa = 4.69 DD198 pKa = 3.7 VVVIDD203 pKa = 6.11 DD204 pKa = 4.49 FYY206 pKa = 11.67 GWLPFCEE213 pKa = 4.47 LLRR216 pKa = 11.84 LTDD219 pKa = 4.49 RR220 pKa = 11.84 YY221 pKa = 8.43 PHH223 pKa = 6.25 KK224 pKa = 11.17 VPVKK228 pKa = 9.5 GSYY231 pKa = 10.41 VEE233 pKa = 5.01 FNSKK237 pKa = 9.4 TIIITSNTHH246 pKa = 5.92 PEE248 pKa = 3.23 TWYY251 pKa = 11.11 NEE253 pKa = 4.13 EE254 pKa = 3.85 KK255 pKa = 10.63 CYY257 pKa = 10.72 LQALFRR263 pKa = 11.84 RR264 pKa = 11.84 INKK267 pKa = 8.27 WMFWDD272 pKa = 3.72 AMRR275 pKa = 11.84 FVDD278 pKa = 6.29 APDD281 pKa = 3.72 CVKK284 pKa = 10.6 KK285 pKa = 11.1 YY286 pKa = 9.74 PINYY290 pKa = 9.36
Molecular weight: 33.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.826
IPC2_protein 8.024
IPC_protein 7.922
Toseland 7.966
ProMoST 8.536
Dawson 8.697
Bjellqvist 8.829
Wikipedia 8.697
Rodwell 8.756
Grimsley 7.892
Solomon 8.814
Lehninger 8.799
Nozaki 8.96
DTASelect 8.639
Thurlkill 8.726
EMBOSS 8.873
Sillero 8.975
Patrickios 4.406
IPC_peptide 8.814
IPC2_peptide 7.6
IPC2.peptide.svr19 7.783
Protein with the highest isoelectric point:
>tr|A0A172MHN5|A0A172MHN5_9CIRC ATP-dependent helicase Rep OS=Avian-like circovirus OX=1844928 PE=3 SV=1
MM1 pKa = 7.47 PPVRR5 pKa = 11.84 AAPAKK10 pKa = 9.69 RR11 pKa = 11.84 WCFTLNNPTDD21 pKa = 3.72 EE22 pKa = 4.39 EE23 pKa = 4.38 VQKK26 pKa = 10.87 IKK28 pKa = 11.16 DD29 pKa = 3.82 MNPSEE34 pKa = 4.01 YY35 pKa = 10.16 HH36 pKa = 4.82 YY37 pKa = 10.93 AIVGKK42 pKa = 10.07 EE43 pKa = 3.83 RR44 pKa = 11.84 GEE46 pKa = 3.88 QGTPHH51 pKa = 6.3 LQGFLHH57 pKa = 6.5 LKK59 pKa = 9.23 CKK61 pKa = 10.31 KK62 pKa = 10.1 RR63 pKa = 11.84 LNQLKK68 pKa = 9.89 EE69 pKa = 4.03 FLARR73 pKa = 11.84 AHH75 pKa = 5.97 WEE77 pKa = 3.8 KK78 pKa = 11.15 ARR80 pKa = 11.84 GSDD83 pKa = 3.67 EE84 pKa = 5.44 DD85 pKa = 3.88 NEE87 pKa = 4.7 AYY89 pKa = 10.06 CSKK92 pKa = 10.06 EE93 pKa = 3.77 GEE95 pKa = 4.45 VILTLGMPAKK105 pKa = 10.49 GNRR108 pKa = 11.84 SDD110 pKa = 4.64 LSGAVAAVKK119 pKa = 10.29 AGRR122 pKa = 11.84 AMSEE126 pKa = 3.74 IARR129 pKa = 11.84 EE130 pKa = 3.83 FSEE133 pKa = 5.75 VYY135 pKa = 10.0 VKK137 pKa = 10.63 YY138 pKa = 10.91 GRR140 pKa = 11.84 GLRR143 pKa = 11.84 DD144 pKa = 3.22 LALLIGQKK152 pKa = 10.21 PRR154 pKa = 11.84 DD155 pKa = 3.85 FKK157 pKa = 11.14 TDD159 pKa = 3.4 VILITGPSGVGKK171 pKa = 10.22 SRR173 pKa = 11.84 WAFEE177 pKa = 4.16 YY178 pKa = 10.24 PGSKK182 pKa = 9.65 FYY184 pKa = 11.33 KK185 pKa = 9.9 MKK187 pKa = 10.93 GEE189 pKa = 3.76 WWDD192 pKa = 3.97 GYY194 pKa = 11.17 ANEE197 pKa = 4.69 DD198 pKa = 3.7 VVVIDD203 pKa = 6.11 DD204 pKa = 4.49 FYY206 pKa = 11.67 GWLPFCEE213 pKa = 4.47 LLRR216 pKa = 11.84 LTDD219 pKa = 4.49 RR220 pKa = 11.84 YY221 pKa = 8.43 PHH223 pKa = 6.25 KK224 pKa = 11.17 VPVKK228 pKa = 9.5 GSYY231 pKa = 10.41 VEE233 pKa = 5.01 FNSKK237 pKa = 9.4 TIIITSNTHH246 pKa = 5.92 PEE248 pKa = 3.23 TWYY251 pKa = 11.11 NEE253 pKa = 4.13 EE254 pKa = 3.85 KK255 pKa = 10.63 CYY257 pKa = 10.72 LQALFRR263 pKa = 11.84 RR264 pKa = 11.84 INKK267 pKa = 8.27 WMFWDD272 pKa = 3.72 AMRR275 pKa = 11.84 FVDD278 pKa = 6.29 APDD281 pKa = 3.72 CVKK284 pKa = 10.6 KK285 pKa = 11.1 YY286 pKa = 9.74 PINYY290 pKa = 9.36
Molecular weight: 33.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.826
IPC2_protein 8.024
IPC_protein 7.922
Toseland 7.966
ProMoST 8.536
Dawson 8.697
Bjellqvist 8.829
Wikipedia 8.697
Rodwell 8.756
Grimsley 7.892
Solomon 8.814
Lehninger 8.799
Nozaki 8.96
DTASelect 8.639
Thurlkill 8.726
EMBOSS 8.873
Sillero 8.975
Patrickios 4.406
IPC_peptide 8.814
IPC2_peptide 7.6
IPC2.peptide.svr19 7.783
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
290
290
290
290.0
33.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.241 ± 0.0
2.069 ± 0.0
5.517 ± 0.0
7.586 ± 0.0
4.483 ± 0.0
7.241 ± 0.0
2.069 ± 0.0
4.483 ± 0.0
8.966 ± 0.0
7.241 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.414 ± 0.0
4.138 ± 0.0
5.517 ± 0.0
2.069 ± 0.0
6.207 ± 0.0
4.483 ± 0.0
3.793 ± 0.0
6.207 ± 0.0
3.103 ± 0.0
5.172 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here