Marinicaulis flavus
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4433 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S7K2I1|A0A2S7K2I1_9PROT Beta-lactamase domain-containing protein OS=Marinicaulis flavus OX=2058213 GN=CW354_14555 PE=4 SV=1
MM1 pKa = 7.17 KK2 pKa = 10.13 HH3 pKa = 6.01 LVSIVAFCFAASVPAAHH20 pKa = 7.0 AAYY23 pKa = 10.53 VGGTSYY29 pKa = 11.51 QDD31 pKa = 3.52 FATDD35 pKa = 3.52 SPFAGADD42 pKa = 3.39 FSGGYY47 pKa = 9.93 FYY49 pKa = 11.45 LEE51 pKa = 4.16 NFEE54 pKa = 5.47 DD55 pKa = 4.27 AALDD59 pKa = 3.91 TPGVSASAGNVASLSGFSGAIIDD82 pKa = 5.7 SVDD85 pKa = 3.48 EE86 pKa = 4.86 DD87 pKa = 4.4 DD88 pKa = 5.58 GSVDD92 pKa = 4.02 GQCVGCDD99 pKa = 2.5 SWFYY103 pKa = 11.47 SSGPTGITFTFNEE116 pKa = 4.41 AVLGALPTHH125 pKa = 6.9 AGIVWTDD132 pKa = 3.35 GANDD136 pKa = 3.38 IQFEE140 pKa = 4.14 AFDD143 pKa = 4.68 ADD145 pKa = 3.94 GDD147 pKa = 4.34 SLGVFGPFMNAGPGVADD164 pKa = 3.38 QDD166 pKa = 4.16 VVEE169 pKa = 4.45 DD170 pKa = 3.91 TFLGVIAATGVSAFHH185 pKa = 6.21 VFSGSAGIEE194 pKa = 4.05 VDD196 pKa = 3.13 HH197 pKa = 6.53 LQYY200 pKa = 11.66 GLANLDD206 pKa = 3.68 GVSDD210 pKa = 4.23 VPVPAALPLFLAGLAGLGAARR231 pKa = 11.84 RR232 pKa = 11.84 RR233 pKa = 11.84 KK234 pKa = 10.02 KK235 pKa = 10.42 RR236 pKa = 11.84 VV237 pKa = 2.86
Molecular weight: 24.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.961
Patrickios 1.1
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A2S7K9P0|A0A2S7K9P0_9PROT Uncharacterized protein OS=Marinicaulis flavus OX=2058213 GN=CW354_04490 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LKK11 pKa = 10.31 RR12 pKa = 11.84 KK13 pKa = 8.93 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.56 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 6.82 ATKK25 pKa = 9.98 GGRR28 pKa = 11.84 KK29 pKa = 9.05 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.301
IPC_protein 12.881
Toseland 13.056
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.778
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.056
DTASelect 13.042
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.501
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.195
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4433
0
4433
1441813
35
3247
325.2
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.799 ± 0.055
0.857 ± 0.011
6.223 ± 0.036
6.565 ± 0.036
4.145 ± 0.027
8.509 ± 0.039
1.839 ± 0.016
5.115 ± 0.025
4.06 ± 0.032
9.369 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.374 ± 0.017
2.888 ± 0.026
4.958 ± 0.024
2.722 ± 0.018
6.56 ± 0.039
5.497 ± 0.027
4.884 ± 0.025
6.77 ± 0.03
1.29 ± 0.014
2.575 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here