Hymenolepis diminuta (Rat tapeworm)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11238 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R3SY24|A0A0R3SY24_HYMDI Anaphase-promoting complex subunit 11 OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS10667 PE=3 SV=1
MM1 pKa = 6.94 IHH3 pKa = 6.74 KK4 pKa = 10.49 NYY6 pKa = 10.24 VLEE9 pKa = 5.23 NEE11 pKa = 4.51 LEE13 pKa = 4.53 SNGGSQCVTEE23 pKa = 4.34 RR24 pKa = 11.84 PEE26 pKa = 4.22 LEE28 pKa = 3.91 SCIDD32 pKa = 3.29 IVQCLLQLDD41 pKa = 3.96 IQFNPNLADD50 pKa = 3.85 CLKK53 pKa = 10.57 ACYY56 pKa = 10.08 EE57 pKa = 4.15 NTQTGHH63 pKa = 5.49 VVDD66 pKa = 6.21 PILEE70 pKa = 4.25 TLAKK74 pKa = 9.63 PAINSIVEE82 pKa = 4.92 FITDD86 pKa = 4.69 PIIDD90 pKa = 4.04 TVNDD94 pKa = 3.61 PTVDD98 pKa = 3.52 LTTDD102 pKa = 3.38 EE103 pKa = 5.46 PIVHH107 pKa = 6.38 PTVDD111 pKa = 3.39 STTDD115 pKa = 3.43 PVIDD119 pKa = 3.95 QPVDD123 pKa = 3.82 SITEE127 pKa = 4.03 PVTAPNFEE135 pKa = 4.42 TPINPTSEE143 pKa = 4.21 TSEE146 pKa = 4.16 SLTDD150 pKa = 3.43 SKK152 pKa = 11.81 VQFTSS157 pKa = 3.03
Molecular weight: 17.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.516
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.427
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.834
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A3P6ZXU4|A0A3P6ZXU4_HYMDI TOG domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS9869 PE=4 SV=1
MM1 pKa = 7.82 LLEE4 pKa = 4.1 QHH6 pKa = 6.41 LHH8 pKa = 4.89 HH9 pKa = 6.96 HH10 pKa = 6.33 LLILILLHH18 pKa = 5.28 HH19 pKa = 6.51 HH20 pKa = 7.21 RR21 pKa = 11.84 ILIHH25 pKa = 5.72 LHH27 pKa = 5.55 HH28 pKa = 6.76 LRR30 pKa = 11.84 ILILLHH36 pKa = 6.14 HH37 pKa = 6.62 LRR39 pKa = 11.84 ILILLHH45 pKa = 6.51 HH46 pKa = 6.83 LLILILLHH54 pKa = 5.41 HH55 pKa = 6.52 HH56 pKa = 7.19 RR57 pKa = 11.84 ILILLHH63 pKa = 6.12 HH64 pKa = 6.62 LRR66 pKa = 11.84 ILILLHH72 pKa = 6.02 HH73 pKa = 6.61 LRR75 pKa = 11.84 ILIHH79 pKa = 5.83 LHH81 pKa = 5.57 HH82 pKa = 6.76 LRR84 pKa = 11.84 ILILLHH90 pKa = 6.02 HH91 pKa = 6.61 LRR93 pKa = 11.84 ILIHH97 pKa = 5.83 LHH99 pKa = 5.57 HH100 pKa = 6.76 LRR102 pKa = 11.84 ILILLHH108 pKa = 6.14 HH109 pKa = 6.62 LRR111 pKa = 11.84 ILILLHH117 pKa = 6.76 HH118 pKa = 6.33 IQILILLHH126 pKa = 6.5 HH127 pKa = 6.86 LLILILLHH135 pKa = 5.41 HH136 pKa = 6.52 HH137 pKa = 7.19 RR138 pKa = 11.84 ILILLHH144 pKa = 6.12 HH145 pKa = 6.62 LRR147 pKa = 11.84 ILILLHH153 pKa = 5.23 HH154 pKa = 6.51 HH155 pKa = 7.19 RR156 pKa = 11.84 ILILLHH162 pKa = 6.12 HH163 pKa = 6.62 LRR165 pKa = 11.84 ILILLHH171 pKa = 6.14 HH172 pKa = 6.62 LRR174 pKa = 11.84 ILILLHH180 pKa = 5.23 HH181 pKa = 6.51 HH182 pKa = 7.19 RR183 pKa = 11.84 ILILLHH189 pKa = 6.12 HH190 pKa = 6.62 LRR192 pKa = 11.84 ILILLHH198 pKa = 6.14 HH199 pKa = 6.62 LRR201 pKa = 11.84 ILILLHH207 pKa = 6.1 HH208 pKa = 6.81 LRR210 pKa = 11.84 IQILLHH216 pKa = 5.79 HH217 pKa = 6.51 LRR219 pKa = 11.84 ILSLLHH225 pKa = 6.33 HH226 pKa = 6.48 LRR228 pKa = 11.84 ILILLHH234 pKa = 6.41
Molecular weight: 28.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.822
Toseland 12.983
ProMoST 13.481
Dawson 12.983
Bjellqvist 12.983
Wikipedia 13.451
Rodwell 12.471
Grimsley 13.013
Solomon 13.481
Lehninger 13.378
Nozaki 12.983
DTASelect 12.983
Thurlkill 12.983
EMBOSS 13.481
Sillero 12.983
Patrickios 12.193
IPC_peptide 13.481
IPC2_peptide 12.471
IPC2.peptide.svr19 9.209
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11237
1
11238
4879823
29
6786
434.2
48.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.559 ± 0.021
1.962 ± 0.018
5.21 ± 0.015
6.473 ± 0.03
4.126 ± 0.017
5.505 ± 0.031
2.3 ± 0.012
5.66 ± 0.018
5.36 ± 0.027
9.473 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.176 ± 0.009
4.719 ± 0.014
5.651 ± 0.028
4.079 ± 0.019
5.833 ± 0.019
9.326 ± 0.034
5.725 ± 0.017
5.98 ± 0.019
1.038 ± 0.008
2.843 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here