Streptococcus satellite phage Javan99

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZZH6|A0A4D5ZZH6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan99 OX=2558871 GN=JavanS99_0015 PE=4 SV=1
MM1 pKa = 7.89PEE3 pKa = 4.21FIIVEE8 pKa = 4.28GNNDD12 pKa = 3.25LGEE15 pKa = 4.18FFRR18 pKa = 11.84IDD20 pKa = 3.85DD21 pKa = 4.19EE22 pKa = 4.55LFSDD26 pKa = 5.27IEE28 pKa = 4.01LLEE31 pKa = 4.04NLKK34 pKa = 10.03KK35 pKa = 8.98WRR37 pKa = 11.84EE38 pKa = 3.84WEE40 pKa = 4.04VAVIIDD46 pKa = 3.79DD47 pKa = 4.11ANRR50 pKa = 11.84TLSEE54 pKa = 4.59DD55 pKa = 3.44EE56 pKa = 4.22TEE58 pKa = 3.83ILYY61 pKa = 10.58FPTHH65 pKa = 6.83EE66 pKa = 5.22DD67 pKa = 3.03NMDD70 pKa = 3.94YY71 pKa = 10.91IRR73 pKa = 11.84VNKK76 pKa = 9.93GLEE79 pKa = 4.06PLYY82 pKa = 10.65HH83 pKa = 6.93ALSKK87 pKa = 10.66PYY89 pKa = 9.15VTISKK94 pKa = 10.3SEE96 pKa = 3.86WLEE99 pKa = 3.82LLDD102 pKa = 4.57

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZX33|A0A4D5ZX33_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan99 OX=2558871 GN=JavanS99_0011 PE=4 SV=1
MM1 pKa = 7.43LNVLFGDD8 pKa = 4.45GKK10 pKa = 10.11HH11 pKa = 6.14RR12 pKa = 11.84GLGRR16 pKa = 11.84VLLVVFKK23 pKa = 10.12SVRR26 pKa = 11.84LPRR29 pKa = 11.84LIYY32 pKa = 9.85LVKK35 pKa = 10.79VSMALFLKK43 pKa = 10.64GAISPPMKK51 pKa = 10.12SVYY54 pKa = 10.03FCLGLL59 pKa = 3.74

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

2464

40

387

136.9

15.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.291 ± 0.515

0.446 ± 0.134

5.195 ± 0.377

8.969 ± 1.112

3.612 ± 0.301

4.505 ± 0.405

1.502 ± 0.188

6.575 ± 0.618

10.268 ± 0.483

10.877 ± 0.747

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.922 ± 0.271

5.601 ± 0.526

2.435 ± 0.258

4.748 ± 0.542

4.424 ± 0.382

5.235 ± 0.393

5.479 ± 0.59

5.844 ± 0.576

1.096 ± 0.215

3.977 ± 0.417
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski