Begomovirus-associated alphasatellite sp.
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D0VPU0|A0A2D0VPU0_9VIRU Alpha rep OS=Begomovirus-associated alphasatellite sp. OX=1911908 GN=gp1 PE=4 SV=1
MM1 pKa = 6.56 SQKK4 pKa = 10.6 RR5 pKa = 11.84 NWCFTIFNYY14 pKa = 9.97 VLPLFTSLPDD24 pKa = 3.13 WANYY28 pKa = 9.14 IVYY31 pKa = 10.0 QEE33 pKa = 4.46 EE34 pKa = 4.35 EE35 pKa = 4.22 CPEE38 pKa = 4.25 TKK40 pKa = 9.85 RR41 pKa = 11.84 KK42 pKa = 9.61 HH43 pKa = 4.61 IQGYY47 pKa = 9.73 VNLKK51 pKa = 9.16 RR52 pKa = 11.84 PQRR55 pKa = 11.84 FSFLKK60 pKa = 10.43 KK61 pKa = 9.93 KK62 pKa = 10.74 LGDD65 pKa = 3.44 GTHH68 pKa = 7.11 IEE70 pKa = 4.22 EE71 pKa = 4.76 CRR73 pKa = 11.84 GSASSNRR80 pKa = 11.84 DD81 pKa = 3.15 YY82 pKa = 10.47 CTKK85 pKa = 10.47 DD86 pKa = 2.88 ASRR89 pKa = 11.84 TGGPWEE95 pKa = 4.17 FGVFCEE101 pKa = 4.38 TGSNKK106 pKa = 9.76 RR107 pKa = 11.84 KK108 pKa = 7.94 TMEE111 pKa = 4.3 RR112 pKa = 11.84 FQEE115 pKa = 4.28 DD116 pKa = 4.08 PEE118 pKa = 4.17 EE119 pKa = 4.61 LRR121 pKa = 11.84 LADD124 pKa = 3.63 PKK126 pKa = 10.93 LYY128 pKa = 10.25 RR129 pKa = 11.84 RR130 pKa = 11.84 CLATKK135 pKa = 10.02 VNKK138 pKa = 9.79 EE139 pKa = 3.67 FSGLVLPIFDD149 pKa = 5.41 RR150 pKa = 11.84 PWQLVAQKK158 pKa = 10.46 ILDD161 pKa = 3.67 QGPDD165 pKa = 3.32 DD166 pKa = 4.01 RR167 pKa = 11.84 TIIWVYY173 pKa = 10.5 GSEE176 pKa = 4.1 GNEE179 pKa = 4.89 GKK181 pKa = 7.15 TTWAKK186 pKa = 10.84 KK187 pKa = 9.95 KK188 pKa = 10.01 IQEE191 pKa = 3.68 GWFYY195 pKa = 11.47 SRR197 pKa = 11.84 GGKK200 pKa = 9.87 GEE202 pKa = 4.13 NIKK205 pKa = 10.35 YY206 pKa = 10.12 QYY208 pKa = 10.88 AEE210 pKa = 4.16 HH211 pKa = 6.98 LGHH214 pKa = 6.82 CVFDD218 pKa = 3.66 IPRR221 pKa = 11.84 QVEE224 pKa = 3.88 DD225 pKa = 3.62 CLQYY229 pKa = 10.81 TVLEE233 pKa = 4.52 EE234 pKa = 3.95 IKK236 pKa = 10.78 DD237 pKa = 3.69 RR238 pKa = 11.84 LIRR241 pKa = 11.84 SSKK244 pKa = 10.56 YY245 pKa = 10.02 EE246 pKa = 4.51 PIDD249 pKa = 3.78 FNCSDD254 pKa = 3.56 KK255 pKa = 11.13 VHH257 pKa = 6.59 VVICSNFLPCIEE269 pKa = 4.91 LEE271 pKa = 4.2 YY272 pKa = 10.91 NNRR275 pKa = 11.84 GEE277 pKa = 4.79 LIKK280 pKa = 10.85 KK281 pKa = 9.5 PLLSRR286 pKa = 11.84 DD287 pKa = 3.15 RR288 pKa = 11.84 VFLININEE296 pKa = 4.56 SVCGHH301 pKa = 7.29 PDD303 pKa = 3.47 DD304 pKa = 4.95 LTAFDD309 pKa = 5.53 LYY311 pKa = 11.38 LSLEE315 pKa = 4.15
Molecular weight: 36.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.306
IPC2_protein 6.427
IPC_protein 6.491
Toseland 6.389
ProMoST 6.707
Dawson 6.985
Bjellqvist 6.985
Wikipedia 6.942
Rodwell 6.985
Grimsley 6.44
Solomon 7.015
Lehninger 7.015
Nozaki 7.322
DTASelect 7.278
Thurlkill 7.307
EMBOSS 7.322
Sillero 7.439
Patrickios 4.113
IPC_peptide 7.015
IPC2_peptide 6.927
IPC2.peptide.svr19 6.838
Protein with the highest isoelectric point:
>tr|A0A2D0VPU0|A0A2D0VPU0_9VIRU Alpha rep OS=Begomovirus-associated alphasatellite sp. OX=1911908 GN=gp1 PE=4 SV=1
MM1 pKa = 6.56 SQKK4 pKa = 10.6 RR5 pKa = 11.84 NWCFTIFNYY14 pKa = 9.97 VLPLFTSLPDD24 pKa = 3.13 WANYY28 pKa = 9.14 IVYY31 pKa = 10.0 QEE33 pKa = 4.46 EE34 pKa = 4.35 EE35 pKa = 4.22 CPEE38 pKa = 4.25 TKK40 pKa = 9.85 RR41 pKa = 11.84 KK42 pKa = 9.61 HH43 pKa = 4.61 IQGYY47 pKa = 9.73 VNLKK51 pKa = 9.16 RR52 pKa = 11.84 PQRR55 pKa = 11.84 FSFLKK60 pKa = 10.43 KK61 pKa = 9.93 KK62 pKa = 10.74 LGDD65 pKa = 3.44 GTHH68 pKa = 7.11 IEE70 pKa = 4.22 EE71 pKa = 4.76 CRR73 pKa = 11.84 GSASSNRR80 pKa = 11.84 DD81 pKa = 3.15 YY82 pKa = 10.47 CTKK85 pKa = 10.47 DD86 pKa = 2.88 ASRR89 pKa = 11.84 TGGPWEE95 pKa = 4.17 FGVFCEE101 pKa = 4.38 TGSNKK106 pKa = 9.76 RR107 pKa = 11.84 KK108 pKa = 7.94 TMEE111 pKa = 4.3 RR112 pKa = 11.84 FQEE115 pKa = 4.28 DD116 pKa = 4.08 PEE118 pKa = 4.17 EE119 pKa = 4.61 LRR121 pKa = 11.84 LADD124 pKa = 3.63 PKK126 pKa = 10.93 LYY128 pKa = 10.25 RR129 pKa = 11.84 RR130 pKa = 11.84 CLATKK135 pKa = 10.02 VNKK138 pKa = 9.79 EE139 pKa = 3.67 FSGLVLPIFDD149 pKa = 5.41 RR150 pKa = 11.84 PWQLVAQKK158 pKa = 10.46 ILDD161 pKa = 3.67 QGPDD165 pKa = 3.32 DD166 pKa = 4.01 RR167 pKa = 11.84 TIIWVYY173 pKa = 10.5 GSEE176 pKa = 4.1 GNEE179 pKa = 4.89 GKK181 pKa = 7.15 TTWAKK186 pKa = 10.84 KK187 pKa = 9.95 KK188 pKa = 10.01 IQEE191 pKa = 3.68 GWFYY195 pKa = 11.47 SRR197 pKa = 11.84 GGKK200 pKa = 9.87 GEE202 pKa = 4.13 NIKK205 pKa = 10.35 YY206 pKa = 10.12 QYY208 pKa = 10.88 AEE210 pKa = 4.16 HH211 pKa = 6.98 LGHH214 pKa = 6.82 CVFDD218 pKa = 3.66 IPRR221 pKa = 11.84 QVEE224 pKa = 3.88 DD225 pKa = 3.62 CLQYY229 pKa = 10.81 TVLEE233 pKa = 4.52 EE234 pKa = 3.95 IKK236 pKa = 10.78 DD237 pKa = 3.69 RR238 pKa = 11.84 LIRR241 pKa = 11.84 SSKK244 pKa = 10.56 YY245 pKa = 10.02 EE246 pKa = 4.51 PIDD249 pKa = 3.78 FNCSDD254 pKa = 3.56 KK255 pKa = 11.13 VHH257 pKa = 6.59 VVICSNFLPCIEE269 pKa = 4.91 LEE271 pKa = 4.2 YY272 pKa = 10.91 NNRR275 pKa = 11.84 GEE277 pKa = 4.79 LIKK280 pKa = 10.85 KK281 pKa = 9.5 PLLSRR286 pKa = 11.84 DD287 pKa = 3.15 RR288 pKa = 11.84 VFLININEE296 pKa = 4.56 SVCGHH301 pKa = 7.29 PDD303 pKa = 3.47 DD304 pKa = 4.95 LTAFDD309 pKa = 5.53 LYY311 pKa = 11.38 LSLEE315 pKa = 4.15
Molecular weight: 36.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.306
IPC2_protein 6.427
IPC_protein 6.491
Toseland 6.389
ProMoST 6.707
Dawson 6.985
Bjellqvist 6.985
Wikipedia 6.942
Rodwell 6.985
Grimsley 6.44
Solomon 7.015
Lehninger 7.015
Nozaki 7.322
DTASelect 7.278
Thurlkill 7.307
EMBOSS 7.322
Sillero 7.439
Patrickios 4.113
IPC_peptide 7.015
IPC2_peptide 6.927
IPC2.peptide.svr19 6.838
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
315
315
315
315.0
36.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.857 ± 0.0
3.81 ± 0.0
6.032 ± 0.0
8.571 ± 0.0
5.079 ± 0.0
6.349 ± 0.0
1.905 ± 0.0
6.032 ± 0.0
7.937 ± 0.0
8.889 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.635 ± 0.0
4.762 ± 0.0
4.762 ± 0.0
3.81 ± 0.0
6.667 ± 0.0
5.714 ± 0.0
4.444 ± 0.0
5.079 ± 0.0
2.222 ± 0.0
4.444 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here