Amino acid dipepetide frequency for Ralstonia phage Jenny

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.615AlaAla: 18.615 ± 1.513
0.908AlaCys: 0.908 ± 0.298
7.34AlaAsp: 7.34 ± 0.881
7.34AlaGlu: 7.34 ± 0.862
4.54AlaPhe: 4.54 ± 0.682
13.016AlaGly: 13.016 ± 1.708
1.665AlaHis: 1.665 ± 0.358
5.221AlaIle: 5.221 ± 0.662
3.859AlaLys: 3.859 ± 0.585
11.578AlaLeu: 11.578 ± 1.219
3.481AlaMet: 3.481 ± 0.478
4.767AlaAsn: 4.767 ± 0.863
6.583AlaPro: 6.583 ± 0.739
6.659AlaGln: 6.659 ± 0.768
9.156AlaArg: 9.156 ± 0.96
6.962AlaSer: 6.962 ± 0.74
7.946AlaThr: 7.946 ± 1.181
7.643AlaVal: 7.643 ± 0.714
1.135AlaTrp: 1.135 ± 0.325
3.405AlaTyr: 3.405 ± 0.549
0.0AlaXaa: 0.0 ± 0.0
Cys
1.059CysAla: 1.059 ± 0.222
0.227CysCys: 0.227 ± 0.13
0.908CysAsp: 0.908 ± 0.328
0.378CysGlu: 0.378 ± 0.181
0.0CysPhe: 0.0 ± 0.0
1.135CysGly: 1.135 ± 0.36
0.303CysHis: 0.303 ± 0.15
0.454CysIle: 0.454 ± 0.187
0.454CysLys: 0.454 ± 0.183
0.53CysLeu: 0.53 ± 0.275
0.076CysMet: 0.076 ± 0.092
0.303CysAsn: 0.303 ± 0.169
0.378CysPro: 0.378 ± 0.222
0.076CysGln: 0.076 ± 0.077
0.757CysArg: 0.757 ± 0.285
0.378CysSer: 0.378 ± 0.161
0.681CysThr: 0.681 ± 0.244
0.757CysVal: 0.757 ± 0.221
0.0CysTrp: 0.0 ± 0.0
0.227CysTyr: 0.227 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
8.021AspAla: 8.021 ± 0.786
0.681AspCys: 0.681 ± 0.198
3.632AspAsp: 3.632 ± 0.547
3.481AspGlu: 3.481 ± 0.583
1.74AspPhe: 1.74 ± 0.278
4.843AspGly: 4.843 ± 0.718
0.454AspHis: 0.454 ± 0.198
2.497AspIle: 2.497 ± 0.566
1.967AspLys: 1.967 ± 0.352
4.843AspLeu: 4.843 ± 0.612
1.589AspMet: 1.589 ± 0.483
1.816AspAsn: 1.816 ± 0.416
4.086AspPro: 4.086 ± 0.703
1.589AspGln: 1.589 ± 0.387
2.27AspArg: 2.27 ± 0.431
2.951AspSer: 2.951 ± 0.464
3.405AspThr: 3.405 ± 0.537
3.935AspVal: 3.935 ± 0.471
1.211AspTrp: 1.211 ± 0.305
1.665AspTyr: 1.665 ± 0.337
0.0AspXaa: 0.0 ± 0.0
Glu
7.113GluAla: 7.113 ± 0.973
0.454GluCys: 0.454 ± 0.258
2.27GluAsp: 2.27 ± 0.522
3.254GluGlu: 3.254 ± 0.53
1.665GluPhe: 1.665 ± 0.316
4.389GluGly: 4.389 ± 0.824
1.211GluHis: 1.211 ± 0.358
2.421GluIle: 2.421 ± 0.458
2.194GluLys: 2.194 ± 0.47
5.146GluLeu: 5.146 ± 0.618
0.908GluMet: 0.908 ± 0.222
2.043GluAsn: 2.043 ± 0.358
1.589GluPro: 1.589 ± 0.373
3.557GluGln: 3.557 ± 0.519
4.313GluArg: 4.313 ± 0.671
2.724GluSer: 2.724 ± 0.471
2.649GluThr: 2.649 ± 0.481
2.421GluVal: 2.421 ± 0.456
0.53GluTrp: 0.53 ± 0.213
1.211GluTyr: 1.211 ± 0.27
0.0GluXaa: 0.0 ± 0.0
Phe
4.238PheAla: 4.238 ± 0.544
0.605PheCys: 0.605 ± 0.243
2.649PheAsp: 2.649 ± 0.484
1.589PheGlu: 1.589 ± 0.33
1.059PhePhe: 1.059 ± 0.345
2.346PheGly: 2.346 ± 0.468
0.53PheHis: 0.53 ± 0.162
1.059PheIle: 1.059 ± 0.241
1.059PheLys: 1.059 ± 0.303
2.724PheLeu: 2.724 ± 0.527
0.53PheMet: 0.53 ± 0.213
1.665PheAsn: 1.665 ± 0.321
1.74PhePro: 1.74 ± 0.471
0.908PheGln: 0.908 ± 0.286
1.665PheArg: 1.665 ± 0.296
1.892PheSer: 1.892 ± 0.401
2.497PheThr: 2.497 ± 0.476
2.649PheVal: 2.649 ± 0.44
0.378PheTrp: 0.378 ± 0.165
1.211PheTyr: 1.211 ± 0.302
0.0PheXaa: 0.0 ± 0.0
Gly
11.351GlyAla: 11.351 ± 1.37
0.984GlyCys: 0.984 ± 0.299
3.481GlyAsp: 3.481 ± 0.43
4.692GlyGlu: 4.692 ± 0.938
4.086GlyPhe: 4.086 ± 0.615
7.87GlyGly: 7.87 ± 2.131
1.135GlyHis: 1.135 ± 0.31
3.784GlyIle: 3.784 ± 0.972
3.254GlyLys: 3.254 ± 0.507
6.356GlyLeu: 6.356 ± 0.682
1.211GlyMet: 1.211 ± 0.304
2.724GlyAsn: 2.724 ± 0.433
2.876GlyPro: 2.876 ± 0.514
2.649GlyGln: 2.649 ± 0.548
4.238GlyArg: 4.238 ± 0.537
3.935GlySer: 3.935 ± 0.81
5.902GlyThr: 5.902 ± 1.071
7.34GlyVal: 7.34 ± 0.642
1.816GlyTrp: 1.816 ± 0.43
2.951GlyTyr: 2.951 ± 0.578
0.0GlyXaa: 0.0 ± 0.0
His
2.27HisAla: 2.27 ± 0.355
0.151HisCys: 0.151 ± 0.11
1.059HisAsp: 1.059 ± 0.291
0.984HisGlu: 0.984 ± 0.331
0.303HisPhe: 0.303 ± 0.132
1.967HisGly: 1.967 ± 0.462
0.227HisHis: 0.227 ± 0.136
0.681HisIle: 0.681 ± 0.249
0.378HisLys: 0.378 ± 0.16
1.665HisLeu: 1.665 ± 0.427
0.227HisMet: 0.227 ± 0.12
0.757HisAsn: 0.757 ± 0.261
0.605HisPro: 0.605 ± 0.234
0.681HisGln: 0.681 ± 0.219
1.135HisArg: 1.135 ± 0.247
1.135HisSer: 1.135 ± 0.307
0.605HisThr: 0.605 ± 0.226
1.362HisVal: 1.362 ± 0.371
0.151HisTrp: 0.151 ± 0.107
0.53HisTyr: 0.53 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
5.524IleAla: 5.524 ± 0.686
0.151IleCys: 0.151 ± 0.112
3.027IleAsp: 3.027 ± 0.375
2.951IleGlu: 2.951 ± 0.533
1.362IlePhe: 1.362 ± 0.307
2.649IleGly: 2.649 ± 0.336
0.832IleHis: 0.832 ± 0.252
1.059IleIle: 1.059 ± 0.265
2.346IleLys: 2.346 ± 0.729
2.497IleLeu: 2.497 ± 0.513
0.984IleMet: 0.984 ± 0.345
1.362IleAsn: 1.362 ± 0.312
2.573IlePro: 2.573 ± 0.387
1.059IleGln: 1.059 ± 0.27
2.27IleArg: 2.27 ± 0.428
2.119IleSer: 2.119 ± 0.423
3.784IleThr: 3.784 ± 0.656
3.632IleVal: 3.632 ± 0.545
0.984IleTrp: 0.984 ± 0.225
1.211IleTyr: 1.211 ± 0.283
0.0IleXaa: 0.0 ± 0.0
Lys
4.238LysAla: 4.238 ± 0.806
0.151LysCys: 0.151 ± 0.11
1.589LysAsp: 1.589 ± 0.29
1.513LysGlu: 1.513 ± 0.289
1.059LysPhe: 1.059 ± 0.272
1.967LysGly: 1.967 ± 0.407
1.135LysHis: 1.135 ± 0.35
1.892LysIle: 1.892 ± 0.388
1.816LysLys: 1.816 ± 0.635
3.935LysLeu: 3.935 ± 0.52
0.908LysMet: 0.908 ± 0.284
0.984LysAsn: 0.984 ± 0.303
2.194LysPro: 2.194 ± 0.345
1.286LysGln: 1.286 ± 0.283
2.497LysArg: 2.497 ± 0.57
1.967LysSer: 1.967 ± 0.298
2.951LysThr: 2.951 ± 0.487
2.497LysVal: 2.497 ± 0.508
0.908LysTrp: 0.908 ± 0.263
0.984LysTyr: 0.984 ± 0.277
0.0LysXaa: 0.0 ± 0.0
Leu
10.443LeuAla: 10.443 ± 1.025
0.908LeuCys: 0.908 ± 0.337
5.675LeuAsp: 5.675 ± 0.747
3.784LeuGlu: 3.784 ± 0.64
2.043LeuPhe: 2.043 ± 0.419
5.675LeuGly: 5.675 ± 0.609
2.194LeuHis: 2.194 ± 0.59
3.027LeuIle: 3.027 ± 0.424
2.421LeuLys: 2.421 ± 0.372
6.432LeuLeu: 6.432 ± 0.966
1.816LeuMet: 1.816 ± 0.338
3.254LeuAsn: 3.254 ± 0.519
5.146LeuPro: 5.146 ± 0.651
3.33LeuGln: 3.33 ± 0.576
6.659LeuArg: 6.659 ± 0.931
5.297LeuSer: 5.297 ± 0.529
5.751LeuThr: 5.751 ± 0.904
6.281LeuVal: 6.281 ± 0.74
1.362LeuTrp: 1.362 ± 0.361
1.967LeuTyr: 1.967 ± 0.43
0.0LeuXaa: 0.0 ± 0.0
Met
3.103MetAla: 3.103 ± 0.529
0.076MetCys: 0.076 ± 0.092
0.832MetAsp: 0.832 ± 0.272
0.605MetGlu: 0.605 ± 0.24
0.454MetPhe: 0.454 ± 0.192
1.286MetGly: 1.286 ± 0.298
0.303MetHis: 0.303 ± 0.151
0.832MetIle: 0.832 ± 0.272
1.438MetLys: 1.438 ± 0.331
1.665MetLeu: 1.665 ± 0.319
0.454MetMet: 0.454 ± 0.22
0.605MetAsn: 0.605 ± 0.219
1.816MetPro: 1.816 ± 0.373
1.286MetGln: 1.286 ± 0.364
1.967MetArg: 1.967 ± 0.427
2.573MetSer: 2.573 ± 0.413
2.194MetThr: 2.194 ± 0.367
0.605MetVal: 0.605 ± 0.22
0.378MetTrp: 0.378 ± 0.173
0.303MetTyr: 0.303 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
4.994AsnAla: 4.994 ± 0.643
0.076AsnCys: 0.076 ± 0.077
2.119AsnAsp: 2.119 ± 0.431
1.74AsnGlu: 1.74 ± 0.388
1.211AsnPhe: 1.211 ± 0.308
2.8AsnGly: 2.8 ± 0.429
0.378AsnHis: 0.378 ± 0.156
1.816AsnIle: 1.816 ± 0.331
1.362AsnLys: 1.362 ± 0.284
2.27AsnLeu: 2.27 ± 0.464
1.286AsnMet: 1.286 ± 0.278
1.589AsnAsn: 1.589 ± 0.413
2.951AsnPro: 2.951 ± 0.5
0.605AsnGln: 0.605 ± 0.24
1.589AsnArg: 1.589 ± 0.315
2.497AsnSer: 2.497 ± 0.492
1.816AsnThr: 1.816 ± 0.359
2.573AsnVal: 2.573 ± 0.473
0.832AsnTrp: 0.832 ± 0.211
1.892AsnTyr: 1.892 ± 0.42
0.0AsnXaa: 0.0 ± 0.0
Pro
7.946ProAla: 7.946 ± 0.801
0.227ProCys: 0.227 ± 0.126
2.649ProAsp: 2.649 ± 0.493
3.405ProGlu: 3.405 ± 0.575
1.665ProPhe: 1.665 ± 0.453
5.448ProGly: 5.448 ± 0.666
0.832ProHis: 0.832 ± 0.275
1.892ProIle: 1.892 ± 0.369
2.194ProLys: 2.194 ± 0.49
3.708ProLeu: 3.708 ± 0.539
0.605ProMet: 0.605 ± 0.241
1.967ProAsn: 1.967 ± 0.362
3.254ProPro: 3.254 ± 0.624
2.497ProGln: 2.497 ± 0.457
1.816ProArg: 1.816 ± 0.401
3.254ProSer: 3.254 ± 0.523
5.146ProThr: 5.146 ± 0.741
3.935ProVal: 3.935 ± 0.406
0.53ProTrp: 0.53 ± 0.232
1.589ProTyr: 1.589 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
6.356GlnAla: 6.356 ± 0.758
0.076GlnCys: 0.076 ± 0.07
2.043GlnAsp: 2.043 ± 0.342
1.589GlnGlu: 1.589 ± 0.439
1.211GlnPhe: 1.211 ± 0.276
2.8GlnGly: 2.8 ± 0.447
0.984GlnHis: 0.984 ± 0.31
3.254GlnIle: 3.254 ± 0.466
1.665GlnLys: 1.665 ± 0.332
3.405GlnLeu: 3.405 ± 0.584
1.362GlnMet: 1.362 ± 0.326
1.589GlnAsn: 1.589 ± 0.388
2.421GlnPro: 2.421 ± 0.357
2.8GlnGln: 2.8 ± 0.566
2.497GlnArg: 2.497 ± 0.416
2.346GlnSer: 2.346 ± 0.503
2.421GlnThr: 2.421 ± 0.474
2.194GlnVal: 2.194 ± 0.393
0.454GlnTrp: 0.454 ± 0.235
0.832GlnTyr: 0.832 ± 0.223
0.0GlnXaa: 0.0 ± 0.0
Arg
8.551ArgAla: 8.551 ± 1.12
0.908ArgCys: 0.908 ± 0.264
4.389ArgAsp: 4.389 ± 0.634
3.935ArgGlu: 3.935 ± 0.57
2.27ArgPhe: 2.27 ± 0.456
3.632ArgGly: 3.632 ± 0.639
1.286ArgHis: 1.286 ± 0.346
1.967ArgIle: 1.967 ± 0.375
2.194ArgLys: 2.194 ± 0.492
4.919ArgLeu: 4.919 ± 0.546
1.892ArgMet: 1.892 ± 0.413
1.967ArgAsn: 1.967 ± 0.34
3.254ArgPro: 3.254 ± 0.459
3.027ArgGln: 3.027 ± 0.516
3.103ArgArg: 3.103 ± 0.538
1.74ArgSer: 1.74 ± 0.455
3.103ArgThr: 3.103 ± 0.486
5.146ArgVal: 5.146 ± 0.668
1.438ArgTrp: 1.438 ± 0.326
2.27ArgTyr: 2.27 ± 0.429
0.0ArgXaa: 0.0 ± 0.0
Ser
6.659SerAla: 6.659 ± 0.731
0.454SerCys: 0.454 ± 0.174
3.027SerAsp: 3.027 ± 0.479
2.724SerGlu: 2.724 ± 0.513
2.119SerPhe: 2.119 ± 0.34
6.508SerGly: 6.508 ± 1.166
0.303SerHis: 0.303 ± 0.18
2.876SerIle: 2.876 ± 0.481
1.816SerLys: 1.816 ± 0.438
4.616SerLeu: 4.616 ± 0.544
1.589SerMet: 1.589 ± 0.281
2.119SerAsn: 2.119 ± 0.376
2.346SerPro: 2.346 ± 0.391
2.421SerGln: 2.421 ± 0.371
2.8SerArg: 2.8 ± 0.433
3.33SerSer: 3.33 ± 0.623
3.784SerThr: 3.784 ± 0.384
4.086SerVal: 4.086 ± 0.668
0.757SerTrp: 0.757 ± 0.243
1.589SerTyr: 1.589 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
7.34ThrAla: 7.34 ± 0.823
0.378ThrCys: 0.378 ± 0.146
3.178ThrAsp: 3.178 ± 0.487
2.724ThrGlu: 2.724 ± 0.525
2.194ThrPhe: 2.194 ± 0.361
5.827ThrGly: 5.827 ± 1.059
0.681ThrHis: 0.681 ± 0.245
3.935ThrIle: 3.935 ± 0.594
2.497ThrLys: 2.497 ± 0.453
5.221ThrLeu: 5.221 ± 0.575
1.362ThrMet: 1.362 ± 0.265
2.573ThrAsn: 2.573 ± 0.467
4.767ThrPro: 4.767 ± 0.646
2.724ThrGln: 2.724 ± 0.541
4.616ThrArg: 4.616 ± 0.679
3.632ThrSer: 3.632 ± 0.795
5.524ThrThr: 5.524 ± 1.123
5.07ThrVal: 5.07 ± 0.777
1.059ThrTrp: 1.059 ± 0.292
2.043ThrTyr: 2.043 ± 0.442
0.0ThrXaa: 0.0 ± 0.0
Val
8.551ValAla: 8.551 ± 0.655
0.757ValCys: 0.757 ± 0.279
4.162ValAsp: 4.162 ± 0.671
3.557ValGlu: 3.557 ± 0.541
2.497ValPhe: 2.497 ± 0.472
4.616ValGly: 4.616 ± 0.568
1.286ValHis: 1.286 ± 0.345
2.27ValIle: 2.27 ± 0.395
2.573ValLys: 2.573 ± 0.484
7.946ValLeu: 7.946 ± 0.592
1.589ValMet: 1.589 ± 0.42
2.27ValAsn: 2.27 ± 0.371
4.086ValPro: 4.086 ± 0.566
3.254ValGln: 3.254 ± 0.56
3.784ValArg: 3.784 ± 0.477
4.313ValSer: 4.313 ± 0.774
4.389ValThr: 4.389 ± 0.75
5.902ValVal: 5.902 ± 0.679
1.059ValTrp: 1.059 ± 0.272
1.892ValTyr: 1.892 ± 0.354
0.0ValXaa: 0.0 ± 0.0
Trp
1.589TrpAla: 1.589 ± 0.495
0.454TrpCys: 0.454 ± 0.19
0.605TrpAsp: 0.605 ± 0.26
0.605TrpGlu: 0.605 ± 0.242
0.681TrpPhe: 0.681 ± 0.233
1.286TrpGly: 1.286 ± 0.28
0.53TrpHis: 0.53 ± 0.216
0.303TrpIle: 0.303 ± 0.164
0.303TrpLys: 0.303 ± 0.153
1.665TrpLeu: 1.665 ± 0.368
0.303TrpMet: 0.303 ± 0.14
0.454TrpAsn: 0.454 ± 0.186
0.454TrpPro: 0.454 ± 0.178
0.757TrpGln: 0.757 ± 0.234
1.74TrpArg: 1.74 ± 0.413
1.059TrpSer: 1.059 ± 0.396
0.984TrpThr: 0.984 ± 0.375
1.059TrpVal: 1.059 ± 0.282
0.378TrpTrp: 0.378 ± 0.167
0.454TrpTyr: 0.454 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.935TyrAla: 3.935 ± 0.501
0.454TyrCys: 0.454 ± 0.174
2.043TyrAsp: 2.043 ± 0.392
1.211TyrGlu: 1.211 ± 0.262
0.832TyrPhe: 0.832 ± 0.276
2.573TyrGly: 2.573 ± 0.531
0.605TyrHis: 0.605 ± 0.295
1.135TyrIle: 1.135 ± 0.26
0.681TyrLys: 0.681 ± 0.27
2.346TyrLeu: 2.346 ± 0.45
0.53TyrMet: 0.53 ± 0.204
1.589TyrAsn: 1.589 ± 0.274
1.438TyrPro: 1.438 ± 0.311
1.135TyrGln: 1.135 ± 0.297
2.119TyrArg: 2.119 ± 0.565
1.816TyrSer: 1.816 ± 0.33
1.816TyrThr: 1.816 ± 0.337
1.665TyrVal: 1.665 ± 0.326
0.303TyrTrp: 0.303 ± 0.134
0.605TyrTyr: 0.605 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13216 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski