Sphingobacteriaceae bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; unclassified Sphingobacteriaceae

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4775 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3UIC8|A0A2A3UIC8_9SPHI Uncharacterized protein OS=Sphingobacteriaceae bacterium OX=2021370 GN=CNR22_23950 PE=4 SV=1
MM1 pKa = 6.49MTDD4 pKa = 3.14IKK6 pKa = 11.03INIQNPDD13 pKa = 3.05NSITTLEE20 pKa = 4.61APTDD24 pKa = 3.55MGLSLMEE31 pKa = 3.96FLKK34 pKa = 11.33GNDD37 pKa = 3.35YY38 pKa = 11.06DD39 pKa = 4.66ILATCGGMALCATCHH54 pKa = 5.93VEE56 pKa = 4.53VVSGFDD62 pKa = 3.41NLSEE66 pKa = 4.38ISDD69 pKa = 4.29DD70 pKa = 3.95EE71 pKa = 4.47YY72 pKa = 12.0AMLDD76 pKa = 3.57TLPNITPTSRR86 pKa = 11.84LACQLRR92 pKa = 11.84LNDD95 pKa = 3.9SLNEE99 pKa = 3.96VTVKK103 pKa = 10.4IMGDD107 pKa = 4.14GII109 pKa = 3.97

Molecular weight: 11.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3U9Y4|A0A2A3U9Y4_9SPHI PKD domain-containing protein OS=Sphingobacteriaceae bacterium OX=2021370 GN=CNR22_11105 PE=4 SV=1
MM1 pKa = 7.66GEE3 pKa = 4.62LAPPLPITIGIQCRR17 pKa = 11.84RR18 pKa = 11.84LLIPIAIGTRR28 pKa = 11.84VLPIAIGSEE37 pKa = 4.14VKK39 pKa = 10.43INYY42 pKa = 9.21AKK44 pKa = 10.88AKK46 pKa = 9.04LCHH49 pKa = 6.22AATRR53 pKa = 11.84HH54 pKa = 3.79QTKK57 pKa = 10.04IAFVYY62 pKa = 10.94AMLSLVRR69 pKa = 11.84GGATFAVMRR78 pKa = 11.84TCFFVRR84 pKa = 11.84LFSS87 pKa = 3.77

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4775

0

4775

1684237

38

3410

352.7

39.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.788 ± 0.047

1.071 ± 0.015

4.828 ± 0.032

5.648 ± 0.061

5.285 ± 0.034

6.523 ± 0.045

1.722 ± 0.017

7.308 ± 0.031

7.641 ± 0.074

9.372 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.113 ± 0.02

6.063 ± 0.04

3.563 ± 0.023

3.225 ± 0.02

3.276 ± 0.031

7.462 ± 0.065

6.594 ± 0.081

6.322 ± 0.032

1.023 ± 0.013

4.175 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski