Streptococcus phage Javan385

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A966|A0A4D6A966_9CAUD HTH_17 domain-containing protein OS=Streptococcus phage Javan385 OX=2548141 GN=Javan385_0042 PE=4 SV=1
MM1 pKa = 7.45SVTPEE6 pKa = 3.93QVISALNLEE15 pKa = 4.32EE16 pKa = 5.26VDD18 pKa = 4.36DD19 pKa = 4.71KK20 pKa = 11.96SLISLYY26 pKa = 10.93LNSATNYY33 pKa = 7.8IKK35 pKa = 10.62KK36 pKa = 10.53AIGQEE41 pKa = 3.69IEE43 pKa = 4.63GYY45 pKa = 9.69PFYY48 pKa = 10.57TLEE51 pKa = 4.31DD52 pKa = 3.8VEE54 pKa = 5.66SEE56 pKa = 3.75FDD58 pKa = 3.46TAVIALTGTYY68 pKa = 7.56YY69 pKa = 10.34TYY71 pKa = 10.74RR72 pKa = 11.84IAVTDD77 pKa = 3.73TQSYY81 pKa = 9.22QMDD84 pKa = 3.74YY85 pKa = 9.68TLNSIVGQLRR95 pKa = 11.84GQYY98 pKa = 10.02ASYY101 pKa = 10.96VEE103 pKa = 4.4EE104 pKa = 4.42VEE106 pKa = 4.3NGQAVSTVV114 pKa = 2.96

Molecular weight: 12.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AAZ3|A0A4D6AAZ3_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan385 OX=2548141 GN=Javan385_0043 PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84TEE4 pKa = 4.6KK5 pKa = 10.86DD6 pKa = 2.71IEE8 pKa = 4.26NYY10 pKa = 10.11LKK12 pKa = 10.73RR13 pKa = 11.84KK14 pKa = 7.91TKK16 pKa = 9.99GLCFKK21 pKa = 8.85FTSPGTVGVPDD32 pKa = 5.01RR33 pKa = 11.84IIVMEE38 pKa = 4.11TGTFFVEE45 pKa = 4.65VKK47 pKa = 10.91APGKK51 pKa = 10.17KK52 pKa = 9.43PRR54 pKa = 11.84PSQIAMHH61 pKa = 6.51KK62 pKa = 10.04KK63 pKa = 9.8

Molecular weight: 7.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12609

44

1502

225.2

25.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.63 ± 0.505

0.476 ± 0.097

6.559 ± 0.34

6.884 ± 0.484

4.187 ± 0.171

6.527 ± 0.554

1.435 ± 0.12

7.162 ± 0.334

8.462 ± 0.386

8.478 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.155

6.099 ± 0.309

3.03 ± 0.165

3.87 ± 0.171

3.331 ± 0.259

6.789 ± 0.375

6.424 ± 0.505

5.916 ± 0.192

1.38 ± 0.117

3.831 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski