Amino acid dipepetide frequency for Nitrososphaera phage Pro-Nvie1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.231AlaAla: 10.231 ± 2.131
0.99AlaCys: 0.99 ± 0.374
6.766AlaAsp: 6.766 ± 1.227
7.921AlaGlu: 7.921 ± 1.363
3.96AlaPhe: 3.96 ± 0.801
8.086AlaGly: 8.086 ± 1.293
1.155AlaHis: 1.155 ± 0.415
4.785AlaIle: 4.785 ± 1.142
4.785AlaLys: 4.785 ± 0.949
6.766AlaLeu: 6.766 ± 1.208
3.135AlaMet: 3.135 ± 0.676
3.3AlaAsn: 3.3 ± 1.296
2.475AlaPro: 2.475 ± 0.799
5.611AlaGln: 5.611 ± 1.247
5.776AlaArg: 5.776 ± 1.118
7.921AlaSer: 7.921 ± 1.475
5.611AlaThr: 5.611 ± 1.143
4.785AlaVal: 4.785 ± 0.667
0.495AlaTrp: 0.495 ± 0.286
2.475AlaTyr: 2.475 ± 0.616
0.0AlaXaa: 0.0 ± 0.0
Cys
0.33CysAla: 0.33 ± 0.207
0.33CysCys: 0.33 ± 0.344
0.33CysAsp: 0.33 ± 0.246
0.495CysGlu: 0.495 ± 0.287
0.495CysPhe: 0.495 ± 0.252
1.815CysGly: 1.815 ± 0.651
0.33CysHis: 0.33 ± 0.266
0.99CysIle: 0.99 ± 0.405
0.495CysLys: 0.495 ± 0.24
0.66CysLeu: 0.66 ± 0.299
0.33CysMet: 0.33 ± 0.191
0.495CysAsn: 0.495 ± 0.244
0.495CysPro: 0.495 ± 0.345
0.0CysGln: 0.0 ± 0.0
0.99CysArg: 0.99 ± 0.538
0.495CysSer: 0.495 ± 0.296
0.33CysThr: 0.33 ± 0.224
0.495CysVal: 0.495 ± 0.259
0.0CysTrp: 0.0 ± 0.0
0.495CysTyr: 0.495 ± 0.243
0.0CysXaa: 0.0 ± 0.0
Asp
7.096AspAla: 7.096 ± 1.06
0.66AspCys: 0.66 ± 0.385
3.63AspAsp: 3.63 ± 0.686
3.3AspGlu: 3.3 ± 0.709
1.98AspPhe: 1.98 ± 0.677
3.96AspGly: 3.96 ± 1.417
0.66AspHis: 0.66 ± 0.28
2.97AspIle: 2.97 ± 0.871
3.465AspLys: 3.465 ± 0.808
6.931AspLeu: 6.931 ± 1.423
1.98AspMet: 1.98 ± 0.617
1.98AspAsn: 1.98 ± 0.54
2.64AspPro: 2.64 ± 0.858
1.32AspGln: 1.32 ± 0.324
4.95AspArg: 4.95 ± 0.892
4.29AspSer: 4.29 ± 0.851
4.785AspThr: 4.785 ± 0.914
4.95AspVal: 4.95 ± 0.676
0.66AspTrp: 0.66 ± 0.498
1.98AspTyr: 1.98 ± 0.649
0.0AspXaa: 0.0 ± 0.0
Glu
7.096GluAla: 7.096 ± 1.143
0.66GluCys: 0.66 ± 0.371
2.805GluAsp: 2.805 ± 0.66
2.97GluGlu: 2.97 ± 0.745
2.145GluPhe: 2.145 ± 0.618
3.135GluGly: 3.135 ± 0.828
0.99GluHis: 0.99 ± 0.466
5.446GluIle: 5.446 ± 1.128
6.766GluLys: 6.766 ± 1.59
4.125GluLeu: 4.125 ± 0.787
1.98GluMet: 1.98 ± 0.539
2.64GluAsn: 2.64 ± 0.601
2.475GluPro: 2.475 ± 0.665
3.3GluGln: 3.3 ± 0.774
3.795GluArg: 3.795 ± 0.798
3.795GluSer: 3.795 ± 0.631
4.29GluThr: 4.29 ± 0.998
4.785GluVal: 4.785 ± 1.05
0.825GluTrp: 0.825 ± 0.363
1.815GluTyr: 1.815 ± 0.637
0.0GluXaa: 0.0 ± 0.0
Phe
2.31PheAla: 2.31 ± 0.557
0.495PheCys: 0.495 ± 0.373
3.3PheAsp: 3.3 ± 0.701
2.145PheGlu: 2.145 ± 0.583
2.31PhePhe: 2.31 ± 0.646
2.805PheGly: 2.805 ± 0.676
0.495PheHis: 0.495 ± 0.283
1.155PheIle: 1.155 ± 0.374
1.32PheLys: 1.32 ± 0.408
2.145PheLeu: 2.145 ± 0.526
0.495PheMet: 0.495 ± 0.261
1.65PheAsn: 1.65 ± 0.603
1.32PhePro: 1.32 ± 0.589
1.65PheGln: 1.65 ± 0.542
2.31PheArg: 2.31 ± 0.659
3.3PheSer: 3.3 ± 1.031
1.815PheThr: 1.815 ± 0.511
2.97PheVal: 2.97 ± 0.775
0.66PheTrp: 0.66 ± 0.267
1.98PheTyr: 1.98 ± 0.616
0.0PheXaa: 0.0 ± 0.0
Gly
8.251GlyAla: 8.251 ± 1.196
0.33GlyCys: 0.33 ± 0.209
3.465GlyAsp: 3.465 ± 0.935
3.135GlyGlu: 3.135 ± 0.824
1.98GlyPhe: 1.98 ± 0.546
5.116GlyGly: 5.116 ± 1.933
1.65GlyHis: 1.65 ± 0.503
5.446GlyIle: 5.446 ± 0.956
1.98GlyLys: 1.98 ± 0.402
5.281GlyLeu: 5.281 ± 1.049
2.145GlyMet: 2.145 ± 0.567
4.455GlyAsn: 4.455 ± 0.737
0.99GlyPro: 0.99 ± 0.42
1.98GlyGln: 1.98 ± 0.54
3.63GlyArg: 3.63 ± 0.701
4.62GlySer: 4.62 ± 1.014
5.281GlyThr: 5.281 ± 1.336
3.96GlyVal: 3.96 ± 0.543
0.66GlyTrp: 0.66 ± 0.396
2.805GlyTyr: 2.805 ± 0.803
0.0GlyXaa: 0.0 ± 0.0
His
1.155HisAla: 1.155 ± 0.469
0.66HisCys: 0.66 ± 0.555
1.32HisAsp: 1.32 ± 0.321
0.825HisGlu: 0.825 ± 0.362
0.495HisPhe: 0.495 ± 0.31
0.66HisGly: 0.66 ± 0.337
0.825HisHis: 0.825 ± 0.381
0.825HisIle: 0.825 ± 0.356
0.66HisLys: 0.66 ± 0.37
2.145HisLeu: 2.145 ± 0.702
0.165HisMet: 0.165 ± 0.149
0.66HisAsn: 0.66 ± 0.355
1.155HisPro: 1.155 ± 0.551
0.0HisGln: 0.0 ± 0.0
1.65HisArg: 1.65 ± 0.542
0.825HisSer: 0.825 ± 0.574
0.99HisThr: 0.99 ± 0.381
0.825HisVal: 0.825 ± 0.344
0.0HisTrp: 0.0 ± 0.0
0.66HisTyr: 0.66 ± 0.322
0.0HisXaa: 0.0 ± 0.0
Ile
4.455IleAla: 4.455 ± 0.817
0.495IleCys: 0.495 ± 0.25
3.465IleAsp: 3.465 ± 0.745
4.455IleGlu: 4.455 ± 0.914
1.155IlePhe: 1.155 ± 0.45
3.3IleGly: 3.3 ± 0.822
0.825IleHis: 0.825 ± 0.467
2.31IleIle: 2.31 ± 0.882
2.64IleLys: 2.64 ± 0.78
4.125IleLeu: 4.125 ± 0.912
1.155IleMet: 1.155 ± 0.344
2.475IleAsn: 2.475 ± 0.498
1.98IlePro: 1.98 ± 0.664
2.145IleGln: 2.145 ± 0.504
3.135IleArg: 3.135 ± 0.733
3.465IleSer: 3.465 ± 0.88
5.611IleThr: 5.611 ± 1.005
3.3IleVal: 3.3 ± 0.735
0.165IleTrp: 0.165 ± 0.172
2.805IleTyr: 2.805 ± 0.658
0.0IleXaa: 0.0 ± 0.0
Lys
4.62LysAla: 4.62 ± 0.959
0.66LysCys: 0.66 ± 0.442
3.63LysAsp: 3.63 ± 0.764
4.125LysGlu: 4.125 ± 1.094
1.155LysPhe: 1.155 ± 0.369
2.145LysGly: 2.145 ± 0.999
1.155LysHis: 1.155 ± 0.528
3.465LysIle: 3.465 ± 0.778
4.785LysLys: 4.785 ± 1.094
4.455LysLeu: 4.455 ± 0.977
1.155LysMet: 1.155 ± 0.458
2.805LysAsn: 2.805 ± 0.64
2.145LysPro: 2.145 ± 0.67
3.135LysGln: 3.135 ± 0.842
3.3LysArg: 3.3 ± 0.693
4.125LysSer: 4.125 ± 0.705
2.64LysThr: 2.64 ± 0.709
4.29LysVal: 4.29 ± 0.861
0.66LysTrp: 0.66 ± 0.291
1.98LysTyr: 1.98 ± 0.47
0.0LysXaa: 0.0 ± 0.0
Leu
11.386LeuAla: 11.386 ± 1.408
0.66LeuCys: 0.66 ± 0.306
6.106LeuAsp: 6.106 ± 0.952
5.446LeuGlu: 5.446 ± 0.818
3.795LeuPhe: 3.795 ± 0.899
4.785LeuGly: 4.785 ± 0.828
0.825LeuHis: 0.825 ± 0.331
2.97LeuIle: 2.97 ± 0.505
4.29LeuLys: 4.29 ± 1.147
5.611LeuLeu: 5.611 ± 1.066
1.32LeuMet: 1.32 ± 0.438
3.465LeuAsn: 3.465 ± 0.591
3.135LeuPro: 3.135 ± 0.651
5.446LeuGln: 5.446 ± 0.934
5.116LeuArg: 5.116 ± 0.841
4.29LeuSer: 4.29 ± 0.841
4.125LeuThr: 4.125 ± 0.771
6.106LeuVal: 6.106 ± 1.076
0.99LeuTrp: 0.99 ± 0.401
2.97LeuTyr: 2.97 ± 0.566
0.0LeuXaa: 0.0 ± 0.0
Met
1.815MetAla: 1.815 ± 0.465
0.495MetCys: 0.495 ± 0.286
0.99MetAsp: 0.99 ± 0.471
2.31MetGlu: 2.31 ± 0.459
0.66MetPhe: 0.66 ± 0.308
1.485MetGly: 1.485 ± 0.626
0.825MetHis: 0.825 ± 0.331
0.33MetIle: 0.33 ± 0.213
1.815MetLys: 1.815 ± 0.458
3.465MetLeu: 3.465 ± 0.92
0.33MetMet: 0.33 ± 0.19
0.99MetAsn: 0.99 ± 0.502
0.99MetPro: 0.99 ± 0.531
0.99MetGln: 0.99 ± 0.623
2.475MetArg: 2.475 ± 0.707
1.98MetSer: 1.98 ± 0.465
1.32MetThr: 1.32 ± 0.523
0.825MetVal: 0.825 ± 0.39
0.165MetTrp: 0.165 ± 0.17
0.495MetTyr: 0.495 ± 0.242
0.0MetXaa: 0.0 ± 0.0
Asn
3.96AsnAla: 3.96 ± 0.763
0.33AsnCys: 0.33 ± 0.181
3.465AsnAsp: 3.465 ± 0.62
3.63AsnGlu: 3.63 ± 0.761
2.97AsnPhe: 2.97 ± 0.589
3.465AsnGly: 3.465 ± 0.833
0.825AsnHis: 0.825 ± 0.333
1.98AsnIle: 1.98 ± 0.412
1.485AsnLys: 1.485 ± 0.404
3.3AsnLeu: 3.3 ± 0.675
0.825AsnMet: 0.825 ± 0.328
1.815AsnAsn: 1.815 ± 0.585
1.485AsnPro: 1.485 ± 0.425
2.475AsnGln: 2.475 ± 0.898
2.97AsnArg: 2.97 ± 0.649
1.98AsnSer: 1.98 ± 0.568
2.475AsnThr: 2.475 ± 0.743
3.3AsnVal: 3.3 ± 1.599
0.66AsnTrp: 0.66 ± 0.232
0.495AsnTyr: 0.495 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
3.3ProAla: 3.3 ± 0.884
0.165ProCys: 0.165 ± 0.179
1.32ProAsp: 1.32 ± 0.613
2.97ProGlu: 2.97 ± 0.883
1.65ProPhe: 1.65 ± 0.406
2.805ProGly: 2.805 ± 0.486
0.66ProHis: 0.66 ± 0.321
1.65ProIle: 1.65 ± 0.497
1.98ProLys: 1.98 ± 0.598
2.805ProLeu: 2.805 ± 0.807
0.66ProMet: 0.66 ± 0.262
1.815ProAsn: 1.815 ± 0.664
1.32ProPro: 1.32 ± 0.53
0.33ProGln: 0.33 ± 0.184
1.65ProArg: 1.65 ± 0.58
3.135ProSer: 3.135 ± 0.664
2.97ProThr: 2.97 ± 0.499
3.135ProVal: 3.135 ± 0.698
0.825ProTrp: 0.825 ± 0.355
1.815ProTyr: 1.815 ± 0.711
0.0ProXaa: 0.0 ± 0.0
Gln
4.125GlnAla: 4.125 ± 0.757
0.495GlnCys: 0.495 ± 0.229
3.63GlnAsp: 3.63 ± 0.736
2.97GlnGlu: 2.97 ± 0.768
1.485GlnPhe: 1.485 ± 0.404
1.98GlnGly: 1.98 ± 0.59
0.99GlnHis: 0.99 ± 0.312
3.135GlnIle: 3.135 ± 1.033
2.145GlnLys: 2.145 ± 0.655
4.455GlnLeu: 4.455 ± 0.869
0.99GlnMet: 0.99 ± 0.413
2.145GlnAsn: 2.145 ± 0.655
1.98GlnPro: 1.98 ± 0.518
1.98GlnGln: 1.98 ± 0.607
1.155GlnArg: 1.155 ± 0.436
2.31GlnSer: 2.31 ± 0.66
2.475GlnThr: 2.475 ± 0.51
3.3GlnVal: 3.3 ± 0.755
0.33GlnTrp: 0.33 ± 0.279
1.815GlnTyr: 1.815 ± 0.606
0.0GlnXaa: 0.0 ± 0.0
Arg
5.611ArgAla: 5.611 ± 0.624
0.495ArgCys: 0.495 ± 0.235
3.795ArgAsp: 3.795 ± 0.922
3.96ArgGlu: 3.96 ± 0.8
3.3ArgPhe: 3.3 ± 0.891
2.805ArgGly: 2.805 ± 0.595
0.66ArgHis: 0.66 ± 0.33
4.455ArgIle: 4.455 ± 0.654
2.64ArgLys: 2.64 ± 0.739
5.776ArgLeu: 5.776 ± 0.74
1.65ArgMet: 1.65 ± 0.564
1.65ArgAsn: 1.65 ± 0.606
2.475ArgPro: 2.475 ± 0.722
4.125ArgGln: 4.125 ± 0.776
3.96ArgArg: 3.96 ± 1.035
2.64ArgSer: 2.64 ± 0.648
1.98ArgThr: 1.98 ± 0.426
3.96ArgVal: 3.96 ± 0.945
0.825ArgTrp: 0.825 ± 0.326
1.98ArgTyr: 1.98 ± 0.85
0.0ArgXaa: 0.0 ± 0.0
Ser
6.766SerAla: 6.766 ± 1.183
0.99SerCys: 0.99 ± 0.457
4.95SerAsp: 4.95 ± 0.654
3.465SerGlu: 3.465 ± 0.841
2.805SerPhe: 2.805 ± 0.659
5.446SerGly: 5.446 ± 1.366
0.825SerHis: 0.825 ± 0.378
3.3SerIle: 3.3 ± 0.547
5.446SerLys: 5.446 ± 0.938
6.436SerLeu: 6.436 ± 1.172
2.805SerMet: 2.805 ± 0.743
2.97SerAsn: 2.97 ± 0.7
2.97SerPro: 2.97 ± 0.718
1.815SerGln: 1.815 ± 0.542
2.97SerArg: 2.97 ± 0.676
7.261SerSer: 7.261 ± 1.233
3.3SerThr: 3.3 ± 0.576
4.125SerVal: 4.125 ± 1.022
0.495SerTrp: 0.495 ± 0.25
1.65SerTyr: 1.65 ± 0.502
0.0SerXaa: 0.0 ± 0.0
Thr
7.096ThrAla: 7.096 ± 1.191
0.33ThrCys: 0.33 ± 0.24
3.3ThrAsp: 3.3 ± 0.907
3.63ThrGlu: 3.63 ± 0.891
2.475ThrPhe: 2.475 ± 0.629
5.776ThrGly: 5.776 ± 1.389
0.33ThrHis: 0.33 ± 0.181
3.3ThrIle: 3.3 ± 0.683
3.3ThrLys: 3.3 ± 0.771
4.29ThrLeu: 4.29 ± 0.775
1.98ThrMet: 1.98 ± 0.741
2.64ThrAsn: 2.64 ± 0.714
3.795ThrPro: 3.795 ± 0.695
2.64ThrGln: 2.64 ± 0.558
1.65ThrArg: 1.65 ± 0.469
4.95ThrSer: 4.95 ± 0.83
3.795ThrThr: 3.795 ± 0.726
3.135ThrVal: 3.135 ± 0.579
0.66ThrTrp: 0.66 ± 0.363
2.31ThrTyr: 2.31 ± 0.604
0.0ThrXaa: 0.0 ± 0.0
Val
4.455ValAla: 4.455 ± 0.657
0.825ValCys: 0.825 ± 0.42
4.62ValAsp: 4.62 ± 0.977
4.62ValGlu: 4.62 ± 0.623
1.155ValPhe: 1.155 ± 0.399
3.135ValGly: 3.135 ± 0.636
1.155ValHis: 1.155 ± 0.575
2.64ValIle: 2.64 ± 0.808
4.455ValLys: 4.455 ± 0.918
7.591ValLeu: 7.591 ± 0.935
0.495ValMet: 0.495 ± 0.252
3.3ValAsn: 3.3 ± 0.899
1.485ValPro: 1.485 ± 0.468
3.465ValGln: 3.465 ± 0.852
4.29ValArg: 4.29 ± 0.706
7.096ValSer: 7.096 ± 1.509
4.125ValThr: 4.125 ± 0.659
4.455ValVal: 4.455 ± 0.703
0.495ValTrp: 0.495 ± 0.37
1.65ValTyr: 1.65 ± 0.475
0.0ValXaa: 0.0 ± 0.0
Trp
0.165TrpAla: 0.165 ± 0.162
0.0TrpCys: 0.0 ± 0.0
0.99TrpAsp: 0.99 ± 0.49
0.495TrpGlu: 0.495 ± 0.259
0.165TrpPhe: 0.165 ± 0.158
0.99TrpGly: 0.99 ± 0.397
0.495TrpHis: 0.495 ± 0.268
0.495TrpIle: 0.495 ± 0.374
0.66TrpLys: 0.66 ± 0.3
0.495TrpLeu: 0.495 ± 0.263
0.495TrpMet: 0.495 ± 0.368
0.66TrpAsn: 0.66 ± 0.308
0.165TrpPro: 0.165 ± 0.157
0.33TrpGln: 0.33 ± 0.215
0.33TrpArg: 0.33 ± 0.24
0.66TrpSer: 0.66 ± 0.352
1.155TrpThr: 1.155 ± 0.491
0.825TrpVal: 0.825 ± 0.266
0.165TrpTrp: 0.165 ± 0.17
0.33TrpTyr: 0.33 ± 0.228
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.805TyrAla: 2.805 ± 0.621
0.33TyrCys: 0.33 ± 0.208
2.31TyrAsp: 2.31 ± 0.486
2.97TyrGlu: 2.97 ± 0.74
0.495TyrPhe: 0.495 ± 0.374
3.465TyrGly: 3.465 ± 0.833
0.825TyrHis: 0.825 ± 0.305
1.815TyrIle: 1.815 ± 0.631
1.32TyrLys: 1.32 ± 0.589
1.98TyrLeu: 1.98 ± 0.521
0.495TyrMet: 0.495 ± 0.254
1.98TyrAsn: 1.98 ± 0.515
1.485TyrPro: 1.485 ± 0.456
1.155TyrGln: 1.155 ± 0.48
2.805TyrArg: 2.805 ± 0.543
1.65TyrSer: 1.65 ± 0.532
2.31TyrThr: 2.31 ± 0.739
1.98TyrVal: 1.98 ± 0.554
0.33TyrTrp: 0.33 ± 0.236
0.99TyrTyr: 0.99 ± 0.383
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 30 proteins (6061 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski