Amino acid dipepetide frequency for Rhodobacter phage RcRhea

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.539AlaAla: 24.539 ± 1.71
0.441AlaCys: 0.441 ± 0.288
8.915AlaAsp: 8.915 ± 0.854
9.71AlaGlu: 9.71 ± 1.056
5.914AlaPhe: 5.914 ± 0.627
12.711AlaGly: 12.711 ± 1.38
1.942AlaHis: 1.942 ± 0.408
5.826AlaIle: 5.826 ± 0.662
4.502AlaLys: 4.502 ± 0.586
12.269AlaLeu: 12.269 ± 0.924
3.354AlaMet: 3.354 ± 0.523
2.648AlaAsn: 2.648 ± 0.481
8.739AlaPro: 8.739 ± 1.187
3.796AlaGln: 3.796 ± 0.596
10.769AlaArg: 10.769 ± 1.031
6.708AlaSer: 6.708 ± 0.742
7.15AlaThr: 7.15 ± 0.909
9.798AlaVal: 9.798 ± 0.754
2.736AlaTrp: 2.736 ± 0.491
1.501AlaTyr: 1.501 ± 0.31
0.0AlaXaa: 0.0 ± 0.0
Cys
0.794CysAla: 0.794 ± 0.256
0.0CysCys: 0.0 ± 0.0
0.706CysAsp: 0.706 ± 0.309
0.265CysGlu: 0.265 ± 0.16
0.441CysPhe: 0.441 ± 0.17
0.883CysGly: 0.883 ± 0.275
0.265CysHis: 0.265 ± 0.157
0.265CysIle: 0.265 ± 0.18
0.177CysLys: 0.177 ± 0.124
0.794CysLeu: 0.794 ± 0.23
0.088CysMet: 0.088 ± 0.089
0.177CysAsn: 0.177 ± 0.117
0.883CysPro: 0.883 ± 0.197
0.088CysGln: 0.088 ± 0.082
0.53CysArg: 0.53 ± 0.218
0.0CysSer: 0.0 ± 0.0
0.265CysThr: 0.265 ± 0.138
0.265CysVal: 0.265 ± 0.145
0.088CysTrp: 0.088 ± 0.074
0.177CysTyr: 0.177 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
8.562AspAla: 8.562 ± 0.741
0.794AspCys: 0.794 ± 0.233
2.383AspAsp: 2.383 ± 0.515
3.178AspGlu: 3.178 ± 0.63
2.736AspPhe: 2.736 ± 0.366
6.885AspGly: 6.885 ± 0.6
1.236AspHis: 1.236 ± 0.291
1.324AspIle: 1.324 ± 0.271
1.854AspLys: 1.854 ± 0.427
8.65AspLeu: 8.65 ± 0.699
1.942AspMet: 1.942 ± 0.331
1.324AspAsn: 1.324 ± 0.375
4.237AspPro: 4.237 ± 0.517
2.118AspGln: 2.118 ± 0.307
4.149AspArg: 4.149 ± 0.546
2.648AspSer: 2.648 ± 0.459
1.942AspThr: 1.942 ± 0.557
5.12AspVal: 5.12 ± 0.614
1.942AspTrp: 1.942 ± 0.448
0.971AspTyr: 0.971 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
7.415GluAla: 7.415 ± 0.907
0.706GluCys: 0.706 ± 0.28
1.677GluAsp: 1.677 ± 0.264
2.03GluGlu: 2.03 ± 0.377
2.56GluPhe: 2.56 ± 0.48
3.884GluGly: 3.884 ± 0.554
0.706GluHis: 0.706 ± 0.274
3.884GluIle: 3.884 ± 0.731
2.118GluLys: 2.118 ± 0.499
2.295GluLeu: 2.295 ± 0.507
2.295GluMet: 2.295 ± 0.438
1.324GluAsn: 1.324 ± 0.306
1.942GluPro: 1.942 ± 0.425
1.324GluGln: 1.324 ± 0.39
4.325GluArg: 4.325 ± 0.543
2.383GluSer: 2.383 ± 0.368
3.707GluThr: 3.707 ± 0.593
5.737GluVal: 5.737 ± 0.697
1.501GluTrp: 1.501 ± 0.261
1.412GluTyr: 1.412 ± 0.426
0.0GluXaa: 0.0 ± 0.0
Phe
3.884PheAla: 3.884 ± 0.474
0.353PheCys: 0.353 ± 0.172
3.001PheAsp: 3.001 ± 0.6
2.383PheGlu: 2.383 ± 0.433
0.706PhePhe: 0.706 ± 0.207
3.707PheGly: 3.707 ± 0.575
0.883PheHis: 0.883 ± 0.237
1.324PheIle: 1.324 ± 0.302
1.236PheLys: 1.236 ± 0.33
2.383PheLeu: 2.383 ± 0.438
0.971PheMet: 0.971 ± 0.229
1.236PheAsn: 1.236 ± 0.28
1.324PhePro: 1.324 ± 0.314
1.412PheGln: 1.412 ± 0.363
2.207PheArg: 2.207 ± 0.523
1.765PheSer: 1.765 ± 0.395
3.001PheThr: 3.001 ± 0.498
1.765PheVal: 1.765 ± 0.313
0.883PheTrp: 0.883 ± 0.2
1.412PheTyr: 1.412 ± 0.301
0.0PheXaa: 0.0 ± 0.0
Gly
10.857GlyAla: 10.857 ± 1.162
0.794GlyCys: 0.794 ± 0.19
6.179GlyAsp: 6.179 ± 0.698
4.59GlyGlu: 4.59 ± 0.811
2.913GlyPhe: 2.913 ± 0.627
12.887GlyGly: 12.887 ± 1.918
1.854GlyHis: 1.854 ± 0.433
2.648GlyIle: 2.648 ± 0.506
4.149GlyLys: 4.149 ± 0.523
8.209GlyLeu: 8.209 ± 0.745
1.942GlyMet: 1.942 ± 0.348
2.295GlyAsn: 2.295 ± 0.488
4.855GlyPro: 4.855 ± 0.827
4.149GlyGln: 4.149 ± 0.648
7.415GlyArg: 7.415 ± 0.776
5.384GlySer: 5.384 ± 0.751
5.737GlyThr: 5.737 ± 0.708
8.915GlyVal: 8.915 ± 0.882
2.472GlyTrp: 2.472 ± 0.622
1.412GlyTyr: 1.412 ± 0.283
0.0GlyXaa: 0.0 ± 0.0
His
1.854HisAla: 1.854 ± 0.4
0.441HisCys: 0.441 ± 0.183
0.794HisAsp: 0.794 ± 0.308
0.706HisGlu: 0.706 ± 0.22
0.618HisPhe: 0.618 ± 0.222
1.854HisGly: 1.854 ± 0.444
0.353HisHis: 0.353 ± 0.176
0.353HisIle: 0.353 ± 0.17
0.441HisLys: 0.441 ± 0.175
2.03HisLeu: 2.03 ± 0.467
0.0HisMet: 0.0 ± 0.0
0.618HisAsn: 0.618 ± 0.214
1.324HisPro: 1.324 ± 0.361
0.794HisGln: 0.794 ± 0.197
1.059HisArg: 1.059 ± 0.238
1.236HisSer: 1.236 ± 0.285
0.618HisThr: 0.618 ± 0.174
1.147HisVal: 1.147 ± 0.28
0.353HisTrp: 0.353 ± 0.159
0.353HisTyr: 0.353 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
5.031IleAla: 5.031 ± 0.725
0.177IleCys: 0.177 ± 0.118
1.942IleAsp: 1.942 ± 0.305
2.913IleGlu: 2.913 ± 0.502
0.353IlePhe: 0.353 ± 0.146
4.325IleGly: 4.325 ± 0.663
0.706IleHis: 0.706 ± 0.245
1.059IleIle: 1.059 ± 0.244
1.942IleLys: 1.942 ± 0.447
3.884IleLeu: 3.884 ± 0.563
0.706IleMet: 0.706 ± 0.209
0.971IleAsn: 0.971 ± 0.246
1.765IlePro: 1.765 ± 0.472
1.059IleGln: 1.059 ± 0.316
3.354IleArg: 3.354 ± 0.591
1.589IleSer: 1.589 ± 0.364
1.854IleThr: 1.854 ± 0.506
2.56IleVal: 2.56 ± 0.576
0.618IleTrp: 0.618 ± 0.228
0.706IleTyr: 0.706 ± 0.237
0.0IleXaa: 0.0 ± 0.0
Lys
6.091LysAla: 6.091 ± 0.919
0.177LysCys: 0.177 ± 0.112
2.472LysAsp: 2.472 ± 0.57
0.883LysGlu: 0.883 ± 0.272
0.618LysPhe: 0.618 ± 0.216
3.531LysGly: 3.531 ± 0.569
0.441LysHis: 0.441 ± 0.206
0.971LysIle: 0.971 ± 0.308
1.236LysLys: 1.236 ± 0.271
2.295LysLeu: 2.295 ± 0.533
0.441LysMet: 0.441 ± 0.196
0.706LysAsn: 0.706 ± 0.201
2.207LysPro: 2.207 ± 0.454
0.441LysGln: 0.441 ± 0.17
2.736LysArg: 2.736 ± 0.508
2.383LysSer: 2.383 ± 0.396
1.942LysThr: 1.942 ± 0.42
2.825LysVal: 2.825 ± 0.331
0.794LysTrp: 0.794 ± 0.268
0.618LysTyr: 0.618 ± 0.183
0.0LysXaa: 0.0 ± 0.0
Leu
12.181LeuAla: 12.181 ± 1.06
0.618LeuCys: 0.618 ± 0.223
6.708LeuAsp: 6.708 ± 0.639
3.531LeuGlu: 3.531 ± 0.51
2.56LeuPhe: 2.56 ± 0.513
6.002LeuGly: 6.002 ± 0.889
2.207LeuHis: 2.207 ± 0.447
3.884LeuIle: 3.884 ± 0.492
3.531LeuLys: 3.531 ± 0.539
5.384LeuLeu: 5.384 ± 0.649
1.147LeuMet: 1.147 ± 0.274
2.295LeuAsn: 2.295 ± 0.427
5.649LeuPro: 5.649 ± 1.084
1.765LeuGln: 1.765 ± 0.447
6.002LeuArg: 6.002 ± 0.62
5.384LeuSer: 5.384 ± 0.752
5.473LeuThr: 5.473 ± 0.5
6.885LeuVal: 6.885 ± 0.697
0.794LeuTrp: 0.794 ± 0.302
0.883LeuTyr: 0.883 ± 0.277
0.0LeuXaa: 0.0 ± 0.0
Met
3.001MetAla: 3.001 ± 0.579
0.0MetCys: 0.0 ± 0.0
0.971MetAsp: 0.971 ± 0.288
1.236MetGlu: 1.236 ± 0.321
0.53MetPhe: 0.53 ± 0.174
1.677MetGly: 1.677 ± 0.362
0.0MetHis: 0.0 ± 0.0
1.059MetIle: 1.059 ± 0.253
0.618MetLys: 0.618 ± 0.235
1.854MetLeu: 1.854 ± 0.443
0.618MetMet: 0.618 ± 0.22
0.971MetAsn: 0.971 ± 0.269
0.706MetPro: 0.706 ± 0.203
0.794MetGln: 0.794 ± 0.2
1.059MetArg: 1.059 ± 0.384
1.412MetSer: 1.412 ± 0.297
1.412MetThr: 1.412 ± 0.209
1.942MetVal: 1.942 ± 0.35
0.265MetTrp: 0.265 ± 0.129
0.088MetTyr: 0.088 ± 0.086
0.0MetXaa: 0.0 ± 0.0
Asn
3.707AsnAla: 3.707 ± 0.479
0.353AsnCys: 0.353 ± 0.14
1.412AsnAsp: 1.412 ± 0.352
0.883AsnGlu: 0.883 ± 0.282
0.883AsnPhe: 0.883 ± 0.259
2.913AsnGly: 2.913 ± 0.458
0.53AsnHis: 0.53 ± 0.198
1.765AsnIle: 1.765 ± 0.455
0.971AsnLys: 0.971 ± 0.282
2.207AsnLeu: 2.207 ± 0.464
0.53AsnMet: 0.53 ± 0.179
0.265AsnAsn: 0.265 ± 0.124
1.501AsnPro: 1.501 ± 0.339
0.441AsnGln: 0.441 ± 0.152
1.059AsnArg: 1.059 ± 0.252
1.059AsnSer: 1.059 ± 0.256
1.324AsnThr: 1.324 ± 0.31
1.412AsnVal: 1.412 ± 0.295
0.53AsnTrp: 0.53 ± 0.167
0.53AsnTyr: 0.53 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
9.886ProAla: 9.886 ± 0.982
0.706ProCys: 0.706 ± 0.248
4.767ProAsp: 4.767 ± 0.742
3.619ProGlu: 3.619 ± 0.549
1.501ProPhe: 1.501 ± 0.349
6.355ProGly: 6.355 ± 0.958
1.059ProHis: 1.059 ± 0.325
1.765ProIle: 1.765 ± 0.41
0.883ProLys: 0.883 ± 0.264
4.943ProLeu: 4.943 ± 0.667
0.53ProMet: 0.53 ± 0.179
1.412ProAsn: 1.412 ± 0.345
4.06ProPro: 4.06 ± 0.731
1.059ProGln: 1.059 ± 0.244
3.354ProArg: 3.354 ± 0.637
3.178ProSer: 3.178 ± 0.514
2.648ProThr: 2.648 ± 0.431
4.943ProVal: 4.943 ± 0.848
1.059ProTrp: 1.059 ± 0.319
1.324ProTyr: 1.324 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
3.707GlnAla: 3.707 ± 0.599
0.0GlnCys: 0.0 ± 0.0
1.501GlnAsp: 1.501 ± 0.38
1.677GlnGlu: 1.677 ± 0.347
1.059GlnPhe: 1.059 ± 0.235
2.472GlnGly: 2.472 ± 0.524
0.088GlnHis: 0.088 ± 0.076
2.472GlnIle: 2.472 ± 0.358
1.412GlnLys: 1.412 ± 0.354
1.765GlnLeu: 1.765 ± 0.421
0.706GlnMet: 0.706 ± 0.255
1.324GlnAsn: 1.324 ± 0.364
1.412GlnPro: 1.412 ± 0.284
0.353GlnGln: 0.353 ± 0.156
1.942GlnArg: 1.942 ± 0.313
1.942GlnSer: 1.942 ± 0.429
1.059GlnThr: 1.059 ± 0.335
2.383GlnVal: 2.383 ± 0.33
0.441GlnTrp: 0.441 ± 0.147
0.265GlnTyr: 0.265 ± 0.112
0.0GlnXaa: 0.0 ± 0.0
Arg
10.681ArgAla: 10.681 ± 1.053
0.441ArgCys: 0.441 ± 0.195
5.826ArgAsp: 5.826 ± 0.81
3.354ArgGlu: 3.354 ± 0.641
3.442ArgPhe: 3.442 ± 0.585
6.973ArgGly: 6.973 ± 0.791
1.236ArgHis: 1.236 ± 0.313
3.531ArgIle: 3.531 ± 0.45
2.472ArgLys: 2.472 ± 0.484
5.649ArgLeu: 5.649 ± 0.715
1.942ArgMet: 1.942 ± 0.322
2.118ArgAsn: 2.118 ± 0.314
3.884ArgPro: 3.884 ± 0.548
2.648ArgGln: 2.648 ± 0.433
6.885ArgArg: 6.885 ± 0.885
2.56ArgSer: 2.56 ± 0.469
2.913ArgThr: 2.913 ± 0.4
4.855ArgVal: 4.855 ± 0.552
1.059ArgTrp: 1.059 ± 0.291
1.501ArgTyr: 1.501 ± 0.303
0.0ArgXaa: 0.0 ± 0.0
Ser
7.415SerAla: 7.415 ± 0.854
0.177SerCys: 0.177 ± 0.125
3.531SerAsp: 3.531 ± 0.457
3.089SerGlu: 3.089 ± 0.581
2.207SerPhe: 2.207 ± 0.504
5.826SerGly: 5.826 ± 0.915
1.059SerHis: 1.059 ± 0.326
1.059SerIle: 1.059 ± 0.242
1.324SerLys: 1.324 ± 0.304
4.59SerLeu: 4.59 ± 0.616
0.618SerMet: 0.618 ± 0.308
0.883SerAsn: 0.883 ± 0.231
3.354SerPro: 3.354 ± 0.613
1.236SerGln: 1.236 ± 0.361
3.001SerArg: 3.001 ± 0.514
2.207SerSer: 2.207 ± 0.4
2.383SerThr: 2.383 ± 0.339
3.796SerVal: 3.796 ± 0.459
1.147SerTrp: 1.147 ± 0.39
1.589SerTyr: 1.589 ± 0.438
0.0SerXaa: 0.0 ± 0.0
Thr
8.209ThrAla: 8.209 ± 0.733
0.441ThrCys: 0.441 ± 0.189
3.354ThrAsp: 3.354 ± 0.481
2.472ThrGlu: 2.472 ± 0.466
2.383ThrPhe: 2.383 ± 0.65
6.355ThrGly: 6.355 ± 0.728
0.53ThrHis: 0.53 ± 0.21
1.765ThrIle: 1.765 ± 0.448
1.589ThrLys: 1.589 ± 0.385
4.325ThrLeu: 4.325 ± 0.743
0.53ThrMet: 0.53 ± 0.209
0.883ThrAsn: 0.883 ± 0.259
4.678ThrPro: 4.678 ± 0.62
0.706ThrGln: 0.706 ± 0.24
4.413ThrArg: 4.413 ± 0.588
2.03ThrSer: 2.03 ± 0.517
4.237ThrThr: 4.237 ± 0.898
3.619ThrVal: 3.619 ± 0.559
0.883ThrTrp: 0.883 ± 0.267
1.147ThrTyr: 1.147 ± 0.301
0.0ThrXaa: 0.0 ± 0.0
Val
10.769ValAla: 10.769 ± 0.872
0.441ValCys: 0.441 ± 0.192
5.473ValAsp: 5.473 ± 0.809
5.296ValGlu: 5.296 ± 0.603
3.089ValPhe: 3.089 ± 0.666
6.62ValGly: 6.62 ± 0.885
0.706ValHis: 0.706 ± 0.213
1.412ValIle: 1.412 ± 0.311
2.736ValLys: 2.736 ± 0.415
6.532ValLeu: 6.532 ± 0.626
1.412ValMet: 1.412 ± 0.323
1.854ValAsn: 1.854 ± 0.31
4.943ValPro: 4.943 ± 0.801
2.736ValGln: 2.736 ± 0.386
6.002ValArg: 6.002 ± 0.689
3.442ValSer: 3.442 ± 0.467
4.413ValThr: 4.413 ± 0.478
7.15ValVal: 7.15 ± 0.962
1.324ValTrp: 1.324 ± 0.262
1.059ValTyr: 1.059 ± 0.292
0.0ValXaa: 0.0 ± 0.0
Trp
3.266TrpAla: 3.266 ± 0.403
0.0TrpCys: 0.0 ± 0.0
1.236TrpAsp: 1.236 ± 0.299
0.441TrpGlu: 0.441 ± 0.185
0.53TrpPhe: 0.53 ± 0.285
1.854TrpGly: 1.854 ± 0.323
0.53TrpHis: 0.53 ± 0.196
0.883TrpIle: 0.883 ± 0.279
0.53TrpLys: 0.53 ± 0.187
0.883TrpLeu: 0.883 ± 0.251
0.177TrpMet: 0.177 ± 0.114
0.618TrpAsn: 0.618 ± 0.258
0.883TrpPro: 0.883 ± 0.234
0.706TrpGln: 0.706 ± 0.275
2.207TrpArg: 2.207 ± 0.401
1.677TrpSer: 1.677 ± 0.394
1.412TrpThr: 1.412 ± 0.29
1.324TrpVal: 1.324 ± 0.247
0.441TrpTrp: 0.441 ± 0.183
0.088TrpTyr: 0.088 ± 0.076
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.118TyrAla: 2.118 ± 0.357
0.088TyrCys: 0.088 ± 0.082
1.236TyrAsp: 1.236 ± 0.205
0.971TyrGlu: 0.971 ± 0.277
0.794TyrPhe: 0.794 ± 0.259
1.942TyrGly: 1.942 ± 0.327
0.618TyrHis: 0.618 ± 0.243
0.265TyrIle: 0.265 ± 0.131
0.088TyrLys: 0.088 ± 0.078
1.942TyrLeu: 1.942 ± 0.37
0.177TyrMet: 0.177 ± 0.148
0.353TyrAsn: 0.353 ± 0.191
0.706TyrPro: 0.706 ± 0.185
0.353TyrGln: 0.353 ± 0.16
1.501TyrArg: 1.501 ± 0.369
1.501TyrSer: 1.501 ± 0.513
1.059TyrThr: 1.059 ± 0.27
0.971TyrVal: 0.971 ± 0.31
0.353TyrTrp: 0.353 ± 0.165
0.353TyrTyr: 0.353 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (11330 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski