Acinetobacter phage phiAB6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Beijerinckvirinae; Friunavirus; Acinetobacter virus phiAB6

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A159BDB5|A0A159BDB5_9CAUD Tail fiber OS=Acinetobacter phage phiAB6 OX=1698439 GN=phiAB6_gp40 PE=1 SV=1
MM1 pKa = 8.01ILEE4 pKa = 4.25GVYY7 pKa = 10.26PSFLKK12 pKa = 10.85GVSQQAPQEE21 pKa = 4.16RR22 pKa = 11.84SDD24 pKa = 3.82GQLGAQLNLLSDD36 pKa = 3.92AVTGLRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GGVKK48 pKa = 10.14FQAKK52 pKa = 8.46LTGIPNSSYY61 pKa = 10.72IRR63 pKa = 11.84LIDD66 pKa = 3.58INGVNYY72 pKa = 10.13IMIVDD77 pKa = 4.67TVTGTLKK84 pKa = 10.3IYY86 pKa = 10.89NFDD89 pKa = 3.51GSLLKK94 pKa = 10.64AHH96 pKa = 5.61QTDD99 pKa = 3.84YY100 pKa = 11.69LKK102 pKa = 11.05ASNGKK107 pKa = 9.45ASIRR111 pKa = 11.84STVSRR116 pKa = 11.84NNCFVLNTEE125 pKa = 4.09QVITKK130 pKa = 8.94TPTGGTNPIPNPSTMGYY147 pKa = 9.4ISIRR151 pKa = 11.84SGQFSKK157 pKa = 10.19MYY159 pKa = 10.62SVDD162 pKa = 3.38IKK164 pKa = 10.92SGSYY168 pKa = 8.9TLSFGVGTSGSEE180 pKa = 3.45AWQATPEE187 pKa = 4.08WVATEE192 pKa = 3.73MEE194 pKa = 4.13NRR196 pKa = 11.84IKK198 pKa = 10.89EE199 pKa = 4.23DD200 pKa = 3.05TTLNARR206 pKa = 11.84YY207 pKa = 7.24TVVRR211 pKa = 11.84EE212 pKa = 4.07GSTVALKK219 pKa = 10.76AKK221 pKa = 10.37SAVDD225 pKa = 3.4TNLLVIEE232 pKa = 4.83SGTGSTYY239 pKa = 10.41IQTSNSSRR247 pKa = 11.84VQGKK251 pKa = 8.59QDD253 pKa = 3.83IIANLPNILDD263 pKa = 3.7KK264 pKa = 11.45YY265 pKa = 9.33IIAVGTVGNSAYY277 pKa = 10.01YY278 pKa = 9.98QYY280 pKa = 11.68NATTSTWKK288 pKa = 10.35EE289 pKa = 3.92CGVYY293 pKa = 9.23EE294 pKa = 4.96APYY297 pKa = 10.56KK298 pKa = 9.36FTNEE302 pKa = 3.79PIYY305 pKa = 10.21WYY307 pKa = 10.53FDD309 pKa = 3.43DD310 pKa = 4.54TDD312 pKa = 4.19TIQVKK317 pKa = 10.55SLDD320 pKa = 3.71IQPRR324 pKa = 11.84TAGDD328 pKa = 3.9DD329 pKa = 4.01DD330 pKa = 5.2NNPLPKK336 pKa = 10.25FVDD339 pKa = 4.52FGITGISAYY348 pKa = 8.0QSRR351 pKa = 11.84LVLLSGSYY359 pKa = 11.38VNMSATADD367 pKa = 3.51FNVYY371 pKa = 8.7MRR373 pKa = 11.84TTVEE377 pKa = 4.22EE378 pKa = 4.35LQDD381 pKa = 4.1DD382 pKa = 4.3DD383 pKa = 5.17PIEE386 pKa = 4.31VSSTALSAAQFEE398 pKa = 4.95YY399 pKa = 10.69AVPYY403 pKa = 10.94NKK405 pKa = 10.41DD406 pKa = 3.41LVLLAQNQQAVIPANSTVLTPKK428 pKa = 9.54TAVIYY433 pKa = 9.5PSSKK437 pKa = 11.07ANISMASEE445 pKa = 4.02PQVVSRR451 pKa = 11.84SLYY454 pKa = 8.15YY455 pKa = 9.88TYY457 pKa = 10.89QRR459 pKa = 11.84GTDD462 pKa = 3.74YY463 pKa = 11.16YY464 pKa = 10.96QVGEE468 pKa = 4.81MIPNAYY474 pKa = 10.43SDD476 pKa = 3.65AQYY479 pKa = 10.95YY480 pKa = 8.69AQNLADD486 pKa = 5.62HH487 pKa = 6.88IPLYY491 pKa = 10.03ATGVCTSITGSTTDD505 pKa = 3.0NMAVFSSDD513 pKa = 3.16QKK515 pKa = 11.22EE516 pKa = 4.34LLVHH520 pKa = 5.96QYY522 pKa = 10.11LWAGEE527 pKa = 4.08DD528 pKa = 3.39RR529 pKa = 11.84PLMSFHH535 pKa = 6.66KK536 pKa = 10.23WEE538 pKa = 4.11LPYY541 pKa = 11.02DD542 pKa = 3.81VLHH545 pKa = 5.82VQFLQEE551 pKa = 3.98YY552 pKa = 8.99LVLFMDD558 pKa = 3.9VGDD561 pKa = 4.48DD562 pKa = 3.91LVVGTVNVQLNQLDD576 pKa = 3.96NKK578 pKa = 9.83PIPFLDD584 pKa = 3.25IYY586 pKa = 10.85QYY588 pKa = 11.56VDD590 pKa = 2.84IVDD593 pKa = 4.16GEE595 pKa = 4.56GTLPEE600 pKa = 4.31FLPEE604 pKa = 4.45GEE606 pKa = 4.58LVAAVYY612 pKa = 10.54SSEE615 pKa = 4.19TMRR618 pKa = 11.84HH619 pKa = 5.59AMVQYY624 pKa = 9.82EE625 pKa = 4.19IEE627 pKa = 4.14GTKK630 pKa = 9.98IKK632 pKa = 10.62CQFNGRR638 pKa = 11.84IYY640 pKa = 10.75LGVPYY645 pKa = 10.02EE646 pKa = 4.26SSLTLTPPFVKK657 pKa = 9.94DD658 pKa = 3.39DD659 pKa = 3.6KK660 pKa = 11.07GRR662 pKa = 11.84VVAGSNSTVVDD673 pKa = 3.45LTMTFKK679 pKa = 10.34STGEE683 pKa = 4.05FEE685 pKa = 4.48YY686 pKa = 10.69HH687 pKa = 6.96VSDD690 pKa = 3.56TYY692 pKa = 11.94GDD694 pKa = 3.76VFDD697 pKa = 5.83GEE699 pKa = 4.6TSAQAWSEE707 pKa = 3.85AQLGYY712 pKa = 9.5TRR714 pKa = 11.84VNTVSDD720 pKa = 3.62VKK722 pKa = 10.48FPCGTLLNSTEE733 pKa = 3.9FSIRR737 pKa = 11.84TTGTTEE743 pKa = 3.85LNIISASYY751 pKa = 10.17NIRR754 pKa = 11.84VPNRR758 pKa = 11.84GRR760 pKa = 11.84RR761 pKa = 11.84HH762 pKa = 5.06LL763 pKa = 3.96

Molecular weight: 84.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A159BD85|A0A159BD85_9CAUD Uncharacterized protein OS=Acinetobacter phage phiAB6 OX=1698439 GN=phiAB6_gp9 PE=4 SV=1
MM1 pKa = 7.54SIKK4 pKa = 10.26GYY6 pKa = 9.62SVIQAYY12 pKa = 10.18VDD14 pKa = 3.45EE15 pKa = 4.83LQEE18 pKa = 3.94LTNYY22 pKa = 7.08EE23 pKa = 3.75QRR25 pKa = 11.84KK26 pKa = 8.56RR27 pKa = 11.84EE28 pKa = 3.72QRR30 pKa = 11.84EE31 pKa = 3.52RR32 pKa = 11.84KK33 pKa = 10.27NIVKK37 pKa = 9.48EE38 pKa = 4.0RR39 pKa = 11.84KK40 pKa = 8.94LDD42 pKa = 3.56RR43 pKa = 11.84RR44 pKa = 11.84SKK46 pKa = 11.0RR47 pKa = 11.84EE48 pKa = 3.63AKK50 pKa = 10.09RR51 pKa = 11.84NAWVV55 pKa = 2.99

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12132

27

1032

269.6

30.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.333 ± 0.375

1.063 ± 0.173

6.264 ± 0.2

6.009 ± 0.262

3.429 ± 0.165

7.402 ± 0.319

2.11 ± 0.198

5.638 ± 0.247

6.644 ± 0.328

8.259 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.687 ± 0.182

5.045 ± 0.283

3.503 ± 0.176

4.723 ± 0.286

4.822 ± 0.271

5.786 ± 0.268

6.067 ± 0.291

6.668 ± 0.251

1.261 ± 0.097

4.286 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski