Mycobacterium phage Emma

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XMJ5|A0A249XMJ5_9CAUD Uncharacterized protein OS=Mycobacterium phage Emma OX=2027893 GN=SEA_EMMA_80 PE=4 SV=1
MM1 pKa = 7.59SGDD4 pKa = 3.12INAEE8 pKa = 3.61GFIRR12 pKa = 11.84YY13 pKa = 9.51GGDD16 pKa = 3.52CTCGAIYY23 pKa = 9.12TYY25 pKa = 10.64GGHH28 pKa = 7.08AEE30 pKa = 4.57PGSFDD35 pKa = 4.3PFCPDD40 pKa = 2.7HH41 pKa = 7.27GEE43 pKa = 4.02AAVVATGEE51 pKa = 4.19EE52 pKa = 4.15AA53 pKa = 3.78

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XMK0|A0A249XMK0_9CAUD AAA ATPase OS=Mycobacterium phage Emma OX=2027893 GN=SEA_EMMA_66 PE=4 SV=1
MM1 pKa = 8.05PDD3 pKa = 3.22RR4 pKa = 11.84YY5 pKa = 10.68GDD7 pKa = 3.63PTPEE11 pKa = 3.56PRR13 pKa = 11.84VFVRR17 pKa = 11.84PKK19 pKa = 10.42VNALTVRR26 pKa = 11.84CSWCKK31 pKa = 9.95AGVGSRR37 pKa = 11.84CVVAGTGVVLRR48 pKa = 11.84RR49 pKa = 11.84SSFHH53 pKa = 6.57DD54 pKa = 3.25VRR56 pKa = 11.84VRR58 pKa = 11.84DD59 pKa = 3.96AEE61 pKa = 4.18LAATGALARR70 pKa = 11.84GRR72 pKa = 11.84MSS74 pKa = 3.0

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

18190

28

1176

166.9

18.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.44 ± 0.463

1.204 ± 0.194

6.658 ± 0.254

5.794 ± 0.308

2.936 ± 0.224

8.785 ± 0.648

2.21 ± 0.195

4.404 ± 0.196

3.233 ± 0.188

7.367 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.32 ± 0.127

3.364 ± 0.151

5.926 ± 0.196

3.452 ± 0.205

6.778 ± 0.403

6.102 ± 0.283

6.861 ± 0.256

7.284 ± 0.264

2.358 ± 0.14

2.523 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski