Amino acid dipepetide frequency for Limnohabitans sp. B9-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.893AlaAla: 13.893 ± 0.275
1.275AlaCys: 1.275 ± 0.045
6.037AlaAsp: 6.037 ± 0.115
5.618AlaGlu: 5.618 ± 0.096
3.838AlaPhe: 3.838 ± 0.077
8.865AlaGly: 8.865 ± 0.189
2.769AlaHis: 2.769 ± 0.072
5.167AlaIle: 5.167 ± 0.101
5.03AlaLys: 5.03 ± 0.103
13.233AlaLeu: 13.233 ± 0.151
3.647AlaMet: 3.647 ± 0.078
3.696AlaAsn: 3.696 ± 0.145
5.061AlaPro: 5.061 ± 0.094
6.37AlaGln: 6.37 ± 0.106
6.139AlaArg: 6.139 ± 0.126
6.973AlaSer: 6.973 ± 0.13
6.188AlaThr: 6.188 ± 0.175
8.408AlaVal: 8.408 ± 0.118
1.802AlaTrp: 1.802 ± 0.049
2.492AlaTyr: 2.492 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
1.108CysAla: 1.108 ± 0.04
0.132CysCys: 0.132 ± 0.011
0.543CysAsp: 0.543 ± 0.024
0.504CysGlu: 0.504 ± 0.025
0.309CysPhe: 0.309 ± 0.02
0.989CysGly: 0.989 ± 0.037
0.279CysHis: 0.279 ± 0.018
0.439CysIle: 0.439 ± 0.021
0.281CysLys: 0.281 ± 0.016
0.863CysLeu: 0.863 ± 0.036
0.259CysMet: 0.259 ± 0.015
0.279CysAsn: 0.279 ± 0.017
0.484CysPro: 0.484 ± 0.025
0.349CysGln: 0.349 ± 0.022
0.433CysArg: 0.433 ± 0.021
0.532CysSer: 0.532 ± 0.021
0.528CysThr: 0.528 ± 0.028
0.801CysVal: 0.801 ± 0.036
0.126CysTrp: 0.126 ± 0.011
0.198CysTyr: 0.198 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.696AspAla: 6.696 ± 0.112
0.421AspCys: 0.421 ± 0.021
2.643AspAsp: 2.643 ± 0.059
2.912AspGlu: 2.912 ± 0.07
2.102AspPhe: 2.102 ± 0.048
4.251AspGly: 4.251 ± 0.105
1.158AspHis: 1.158 ± 0.031
2.646AspIle: 2.646 ± 0.06
2.153AspLys: 2.153 ± 0.054
5.371AspLeu: 5.371 ± 0.082
1.52AspMet: 1.52 ± 0.043
1.523AspAsn: 1.523 ± 0.049
2.376AspPro: 2.376 ± 0.052
1.786AspGln: 1.786 ± 0.043
2.619AspArg: 2.619 ± 0.054
2.299AspSer: 2.299 ± 0.061
2.949AspThr: 2.949 ± 0.103
4.618AspVal: 4.618 ± 0.085
0.859AspTrp: 0.859 ± 0.031
1.392AspTyr: 1.392 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
5.993GluAla: 5.993 ± 0.123
0.38GluCys: 0.38 ± 0.025
2.119GluAsp: 2.119 ± 0.054
2.262GluGlu: 2.262 ± 0.064
1.859GluPhe: 1.859 ± 0.049
3.586GluGly: 3.586 ± 0.078
1.249GluHis: 1.249 ± 0.044
2.665GluIle: 2.665 ± 0.067
2.255GluLys: 2.255 ± 0.062
5.499GluLeu: 5.499 ± 0.109
1.382GluMet: 1.382 ± 0.04
1.479GluAsn: 1.479 ± 0.042
2.089GluPro: 2.089 ± 0.06
2.389GluGln: 2.389 ± 0.061
3.614GluArg: 3.614 ± 0.075
2.446GluSer: 2.446 ± 0.054
2.398GluThr: 2.398 ± 0.055
4.104GluVal: 4.104 ± 0.088
0.652GluTrp: 0.652 ± 0.028
0.945GluTyr: 0.945 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
4.029PheAla: 4.029 ± 0.075
0.416PheCys: 0.416 ± 0.024
2.524PheAsp: 2.524 ± 0.055
2.181PheGlu: 2.181 ± 0.049
1.423PhePhe: 1.423 ± 0.053
3.308PheGly: 3.308 ± 0.066
0.703PheHis: 0.703 ± 0.026
1.603PheIle: 1.603 ± 0.044
1.668PheLys: 1.668 ± 0.048
2.95PheLeu: 2.95 ± 0.062
0.933PheMet: 0.933 ± 0.034
1.394PheAsn: 1.394 ± 0.039
1.433PhePro: 1.433 ± 0.041
1.139PheGln: 1.139 ± 0.039
1.489PheArg: 1.489 ± 0.04
2.289PheSer: 2.289 ± 0.052
2.208PheThr: 2.208 ± 0.059
2.907PheVal: 2.907 ± 0.061
0.521PheTrp: 0.521 ± 0.024
0.95PheTyr: 0.95 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
8.06GlyAla: 8.06 ± 0.175
0.885GlyCys: 0.885 ± 0.036
3.827GlyAsp: 3.827 ± 0.09
3.896GlyGlu: 3.896 ± 0.078
3.309GlyPhe: 3.309 ± 0.059
6.662GlyGly: 6.662 ± 0.252
1.928GlyHis: 1.928 ± 0.05
3.996GlyIle: 3.996 ± 0.071
3.622GlyLys: 3.622 ± 0.072
8.525GlyLeu: 8.525 ± 0.114
2.264GlyMet: 2.264 ± 0.05
2.701GlyAsn: 2.701 ± 0.17
2.735GlyPro: 2.735 ± 0.055
3.758GlyGln: 3.758 ± 0.071
3.985GlyArg: 3.985 ± 0.085
4.721GlySer: 4.721 ± 0.173
4.768GlyThr: 4.768 ± 0.254
6.679GlyVal: 6.679 ± 0.101
1.229GlyTrp: 1.229 ± 0.042
2.291GlyTyr: 2.291 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.835HisAla: 2.835 ± 0.064
0.282HisCys: 0.282 ± 0.017
1.231HisAsp: 1.231 ± 0.045
1.053HisGlu: 1.053 ± 0.032
0.869HisPhe: 0.869 ± 0.032
1.985HisGly: 1.985 ± 0.051
0.694HisHis: 0.694 ± 0.031
1.2HisIle: 1.2 ± 0.042
0.792HisLys: 0.792 ± 0.028
2.416HisLeu: 2.416 ± 0.063
0.623HisMet: 0.623 ± 0.028
0.71HisAsn: 0.71 ± 0.029
1.378HisPro: 1.378 ± 0.041
0.89HisGln: 0.89 ± 0.032
1.252HisArg: 1.252 ± 0.041
1.095HisSer: 1.095 ± 0.042
1.39HisThr: 1.39 ± 0.039
1.805HisVal: 1.805 ± 0.048
0.419HisTrp: 0.419 ± 0.023
0.651HisTyr: 0.651 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.667IleAla: 5.667 ± 0.079
0.463IleCys: 0.463 ± 0.02
3.152IleAsp: 3.152 ± 0.068
3.039IleGlu: 3.039 ± 0.07
1.424IlePhe: 1.424 ± 0.045
3.903IleGly: 3.903 ± 0.077
1.038IleHis: 1.038 ± 0.036
1.817IleIle: 1.817 ± 0.056
1.993IleLys: 1.993 ± 0.051
3.405IleLeu: 3.405 ± 0.068
0.902IleMet: 0.902 ± 0.034
1.955IleAsn: 1.955 ± 0.073
2.056IlePro: 2.056 ± 0.052
1.781IleGln: 1.781 ± 0.042
2.442IleArg: 2.442 ± 0.055
2.965IleSer: 2.965 ± 0.067
3.099IleThr: 3.099 ± 0.089
3.334IleVal: 3.334 ± 0.059
0.528IleTrp: 0.528 ± 0.024
1.143IleTyr: 1.143 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.938LysAla: 4.938 ± 0.102
0.219LysCys: 0.219 ± 0.016
2.149LysAsp: 2.149 ± 0.05
1.849LysGlu: 1.849 ± 0.055
1.405LysPhe: 1.405 ± 0.039
3.011LysGly: 3.011 ± 0.062
0.885LysHis: 0.885 ± 0.032
1.944LysIle: 1.944 ± 0.042
2.003LysLys: 2.003 ± 0.066
4.326LysLeu: 4.326 ± 0.091
1.029LysMet: 1.029 ± 0.033
1.445LysAsn: 1.445 ± 0.046
2.491LysPro: 2.491 ± 0.068
1.597LysGln: 1.597 ± 0.043
2.444LysArg: 2.444 ± 0.063
2.343LysSer: 2.343 ± 0.048
2.617LysThr: 2.617 ± 0.049
3.14LysVal: 3.14 ± 0.067
0.437LysTrp: 0.437 ± 0.022
0.846LysTyr: 0.846 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
12.52LeuAla: 12.52 ± 0.128
1.019LeuCys: 1.019 ± 0.034
5.468LeuAsp: 5.468 ± 0.099
4.713LeuGlu: 4.713 ± 0.107
3.239LeuPhe: 3.239 ± 0.071
8.268LeuGly: 8.268 ± 0.135
2.38LeuHis: 2.38 ± 0.064
4.666LeuIle: 4.666 ± 0.086
4.622LeuLys: 4.622 ± 0.088
9.979LeuLeu: 9.979 ± 0.153
3.003LeuMet: 3.003 ± 0.072
3.801LeuAsn: 3.801 ± 0.072
5.472LeuPro: 5.472 ± 0.093
4.718LeuGln: 4.718 ± 0.088
6.046LeuArg: 6.046 ± 0.127
6.998LeuSer: 6.998 ± 0.112
6.201LeuThr: 6.201 ± 0.135
7.446LeuVal: 7.446 ± 0.106
1.261LeuTrp: 1.261 ± 0.049
1.909LeuTyr: 1.909 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
3.345MetAla: 3.345 ± 0.069
0.243MetCys: 0.243 ± 0.017
1.284MetAsp: 1.284 ± 0.042
1.032MetGlu: 1.032 ± 0.033
0.846MetPhe: 0.846 ± 0.032
2.323MetGly: 2.323 ± 0.054
0.667MetHis: 0.667 ± 0.027
1.005MetIle: 1.005 ± 0.037
1.258MetLys: 1.258 ± 0.04
2.695MetLeu: 2.695 ± 0.064
0.685MetMet: 0.685 ± 0.032
1.099MetAsn: 1.099 ± 0.031
1.584MetPro: 1.584 ± 0.043
1.34MetGln: 1.34 ± 0.043
1.709MetArg: 1.709 ± 0.04
1.914MetSer: 1.914 ± 0.051
1.852MetThr: 1.852 ± 0.042
2.051MetVal: 2.051 ± 0.055
0.271MetTrp: 0.271 ± 0.016
0.426MetTyr: 0.426 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
4.195AsnAla: 4.195 ± 0.167
0.27AsnCys: 0.27 ± 0.017
1.746AsnAsp: 1.746 ± 0.076
1.445AsnGlu: 1.445 ± 0.038
1.18AsnPhe: 1.18 ± 0.035
2.863AsnGly: 2.863 ± 0.146
0.641AsnHis: 0.641 ± 0.026
1.647AsnIle: 1.647 ± 0.044
1.268AsnLys: 1.268 ± 0.047
3.386AsnLeu: 3.386 ± 0.085
0.827AsnMet: 0.827 ± 0.028
1.262AsnAsn: 1.262 ± 0.077
2.122AsnPro: 2.122 ± 0.047
1.186AsnGln: 1.186 ± 0.036
1.665AsnArg: 1.665 ± 0.045
1.773AsnSer: 1.773 ± 0.104
2.284AsnThr: 2.284 ± 0.132
2.601AsnVal: 2.601 ± 0.079
0.475AsnTrp: 0.475 ± 0.022
0.873AsnTyr: 0.873 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
5.483ProAla: 5.483 ± 0.086
0.388ProCys: 0.388 ± 0.019
2.865ProAsp: 2.865 ± 0.06
3.168ProGlu: 3.168 ± 0.072
1.783ProPhe: 1.783 ± 0.048
3.424ProGly: 3.424 ± 0.066
1.162ProHis: 1.162 ± 0.042
2.046ProIle: 2.046 ± 0.048
2.04ProLys: 2.04 ± 0.057
4.561ProLeu: 4.561 ± 0.081
1.44ProMet: 1.44 ± 0.042
1.744ProAsn: 1.744 ± 0.041
1.876ProPro: 1.876 ± 0.056
2.133ProGln: 2.133 ± 0.049
2.183ProArg: 2.183 ± 0.052
2.951ProSer: 2.951 ± 0.058
2.796ProThr: 2.796 ± 0.063
3.948ProVal: 3.948 ± 0.071
0.73ProTrp: 0.73 ± 0.032
1.164ProTyr: 1.164 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
6.097GlnAla: 6.097 ± 0.101
0.368GlnCys: 0.368 ± 0.023
2.029GlnAsp: 2.029 ± 0.048
1.838GlnGlu: 1.838 ± 0.049
1.447GlnPhe: 1.447 ± 0.037
3.546GlnGly: 3.546 ± 0.057
1.221GlnHis: 1.221 ± 0.041
2.026GlnIle: 2.026 ± 0.045
1.55GlnLys: 1.55 ± 0.048
4.485GlnLeu: 4.485 ± 0.074
1.122GlnMet: 1.122 ± 0.035
1.218GlnAsn: 1.218 ± 0.034
2.12GlnPro: 2.12 ± 0.049
2.139GlnGln: 2.139 ± 0.062
3.097GlnArg: 3.097 ± 0.078
2.488GlnSer: 2.488 ± 0.058
2.792GlnThr: 2.792 ± 0.061
3.455GlnVal: 3.455 ± 0.063
0.696GlnTrp: 0.696 ± 0.032
0.822GlnTyr: 0.822 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
5.635ArgAla: 5.635 ± 0.099
0.504ArgCys: 0.504 ± 0.025
3.046ArgAsp: 3.046 ± 0.072
3.293ArgGlu: 3.293 ± 0.079
2.275ArgPhe: 2.275 ± 0.058
3.575ArgGly: 3.575 ± 0.061
1.438ArgHis: 1.438 ± 0.048
2.981ArgIle: 2.981 ± 0.061
1.956ArgLys: 1.956 ± 0.064
6.156ArgLeu: 6.156 ± 0.13
1.772ArgMet: 1.772 ± 0.043
1.698ArgAsn: 1.698 ± 0.046
2.25ArgPro: 2.25 ± 0.059
2.515ArgGln: 2.515 ± 0.061
3.107ArgArg: 3.107 ± 0.069
2.957ArgSer: 2.957 ± 0.069
2.79ArgThr: 2.79 ± 0.054
4.419ArgVal: 4.419 ± 0.085
0.907ArgTrp: 0.907 ± 0.032
1.538ArgTyr: 1.538 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.837SerAla: 6.837 ± 0.172
0.493SerCys: 0.493 ± 0.023
2.932SerAsp: 2.932 ± 0.077
2.679SerGlu: 2.679 ± 0.058
2.28SerPhe: 2.28 ± 0.047
5.495SerGly: 5.495 ± 0.199
1.345SerHis: 1.345 ± 0.035
2.601SerIle: 2.601 ± 0.059
2.149SerLys: 2.149 ± 0.053
6.133SerLeu: 6.133 ± 0.083
1.573SerMet: 1.573 ± 0.038
1.999SerAsn: 1.999 ± 0.091
2.924SerPro: 2.924 ± 0.055
2.408SerGln: 2.408 ± 0.056
3.001SerArg: 3.001 ± 0.06
3.571SerSer: 3.571 ± 0.144
3.623SerThr: 3.623 ± 0.16
4.471SerVal: 4.471 ± 0.098
0.804SerTrp: 0.804 ± 0.029
1.43SerTyr: 1.43 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
6.404ThrAla: 6.404 ± 0.237
0.475ThrCys: 0.475 ± 0.026
2.925ThrAsp: 2.925 ± 0.093
2.604ThrGlu: 2.604 ± 0.055
2.152ThrPhe: 2.152 ± 0.053
5.052ThrGly: 5.052 ± 0.224
1.515ThrHis: 1.515 ± 0.044
2.49ThrIle: 2.49 ± 0.088
1.751ThrLys: 1.751 ± 0.05
7.035ThrLeu: 7.035 ± 0.225
1.248ThrMet: 1.248 ± 0.038
1.753ThrAsn: 1.753 ± 0.091
3.798ThrPro: 3.798 ± 0.076
2.84ThrGln: 2.84 ± 0.056
2.993ThrArg: 2.993 ± 0.071
3.526ThrSer: 3.526 ± 0.127
3.548ThrThr: 3.548 ± 0.207
4.787ThrVal: 4.787 ± 0.174
0.792ThrTrp: 0.792 ± 0.03
1.298ThrTyr: 1.298 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
8.869ValAla: 8.869 ± 0.108
0.772ValCys: 0.772 ± 0.031
3.955ValAsp: 3.955 ± 0.079
3.675ValGlu: 3.675 ± 0.063
2.788ValPhe: 2.788 ± 0.063
5.748ValGly: 5.748 ± 0.104
1.666ValHis: 1.666 ± 0.043
3.716ValIle: 3.716 ± 0.062
3.308ValLys: 3.308 ± 0.07
8.467ValLeu: 8.467 ± 0.119
2.452ValMet: 2.452 ± 0.056
2.767ValAsn: 2.767 ± 0.112
3.868ValPro: 3.868 ± 0.079
3.389ValGln: 3.389 ± 0.068
4.281ValArg: 4.281 ± 0.085
4.785ValSer: 4.785 ± 0.132
4.696ValThr: 4.696 ± 0.17
6.77ValVal: 6.77 ± 0.089
1.109ValTrp: 1.109 ± 0.04
1.582ValTyr: 1.582 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
1.338TrpAla: 1.338 ± 0.041
0.171TrpCys: 0.171 ± 0.013
0.581TrpAsp: 0.581 ± 0.022
0.475TrpGlu: 0.475 ± 0.022
0.536TrpPhe: 0.536 ± 0.024
0.993TrpGly: 0.993 ± 0.028
0.415TrpHis: 0.415 ± 0.02
0.61TrpIle: 0.61 ± 0.026
0.428TrpLys: 0.428 ± 0.022
2.024TrpLeu: 2.024 ± 0.059
0.457TrpMet: 0.457 ± 0.024
0.411TrpAsn: 0.411 ± 0.021
0.667TrpPro: 0.667 ± 0.03
0.877TrpGln: 0.877 ± 0.037
0.999TrpArg: 0.999 ± 0.039
0.769TrpSer: 0.769 ± 0.027
0.782TrpThr: 0.782 ± 0.033
1.194TrpVal: 1.194 ± 0.041
0.253TrpTrp: 0.253 ± 0.014
0.257TrpTyr: 0.257 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.559TyrAla: 2.559 ± 0.056
0.261TyrCys: 0.261 ± 0.018
1.239TyrAsp: 1.239 ± 0.04
1.124TyrGlu: 1.124 ± 0.037
1.003TyrPhe: 1.003 ± 0.035
1.994TyrGly: 1.994 ± 0.052
0.417TyrHis: 0.417 ± 0.021
0.839TyrIle: 0.839 ± 0.031
0.993TyrLys: 0.993 ± 0.035
2.222TyrLeu: 2.222 ± 0.056
0.491TyrMet: 0.491 ± 0.022
0.778TyrAsn: 0.778 ± 0.036
1.101TyrPro: 1.101 ± 0.032
0.952TyrGln: 0.952 ± 0.03
1.31TyrArg: 1.31 ± 0.036
1.353TyrSer: 1.353 ± 0.053
1.477TyrThr: 1.477 ± 0.059
1.725TyrVal: 1.725 ± 0.036
0.367TyrTrp: 0.367 ± 0.02
0.54TyrTyr: 0.54 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3143 proteins (1050257 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski