Leucothrix arctica
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4192 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A317CST0|A0A317CST0_9GAMM 3D-(3 5/4)-trihydroxycyclohexane-1 2-dione acylhydrolase (Decyclizing) OS=Leucothrix arctica OX=1481894 GN=iolD PE=3 SV=1
MM1 pKa = 7.83 KK2 pKa = 10.24 LRR4 pKa = 11.84 PFLYY8 pKa = 10.19 SATAMLSLNGAASAEE23 pKa = 4.55 LIFSEE28 pKa = 4.6 YY29 pKa = 10.28 IEE31 pKa = 4.78 GSGNNKK37 pKa = 9.7 ALEE40 pKa = 4.52 IYY42 pKa = 10.35 NSSTADD48 pKa = 3.21 VDD50 pKa = 3.87 LSTYY54 pKa = 10.94 SVVGYY59 pKa = 10.07 QNGGADD65 pKa = 3.09 AGYY68 pKa = 10.55 SVDD71 pKa = 5.11 LSGTLTAGSVYY82 pKa = 10.31 IIAHH86 pKa = 6.05 SSADD90 pKa = 3.55 AEE92 pKa = 4.29 ILAKK96 pKa = 10.24 ADD98 pKa = 3.4 QQYY101 pKa = 10.89 GLQFNGDD108 pKa = 3.92 DD109 pKa = 4.75 AVTLQNSGTIVDD121 pKa = 4.52 SIGKK125 pKa = 9.48 IGEE128 pKa = 4.42 DD129 pKa = 3.89 PGSEE133 pKa = 3.79 WGSGDD138 pKa = 3.75 STTKK142 pKa = 10.73 DD143 pKa = 2.84 HH144 pKa = 6.16 TLRR147 pKa = 11.84 RR148 pKa = 11.84 QAGLTTGDD156 pKa = 3.48 TDD158 pKa = 3.54 ATDD161 pKa = 4.75 DD162 pKa = 4.81 FDD164 pKa = 6.42 PATQWDD170 pKa = 4.39 AFEE173 pKa = 5.73 KK174 pKa = 10.83 DD175 pKa = 3.58 DD176 pKa = 4.04 FSGIGAHH183 pKa = 6.56 GSLVVSDD190 pKa = 4.55 GLPVANAGTDD200 pKa = 3.36 QSGITAGAVVTLDD213 pKa = 3.33 GSGSVDD219 pKa = 3.15 TSEE222 pKa = 4.42 GGSIIDD228 pKa = 4.02 YY229 pKa = 10.68 NWTQTSGTTVALDD242 pKa = 3.62 TTDD245 pKa = 3.0 PTMPSFTTPLAEE257 pKa = 5.53 DD258 pKa = 3.93 SLTFMLTVTDD268 pKa = 3.82 NDD270 pKa = 4.09 GNTATDD276 pKa = 3.57 TVTVNVNAVLISQCNAQTHH295 pKa = 6.21 VISQIQGSGDD305 pKa = 3.39 TSPLVDD311 pKa = 3.63 EE312 pKa = 4.87 VVTVQAVVTKK322 pKa = 10.0 LLPEE326 pKa = 3.87 LTGYY330 pKa = 9.06 MIQEE334 pKa = 4.0 EE335 pKa = 4.35 AADD338 pKa = 4.14 EE339 pKa = 4.93 DD340 pKa = 4.34 GDD342 pKa = 4.03 AATSEE347 pKa = 4.58 GVFVYY352 pKa = 10.19 DD353 pKa = 3.73 YY354 pKa = 11.4 ANSPSVGDD362 pKa = 3.54 VVSIVGTVAEE372 pKa = 4.84 YY373 pKa = 11.56 YY374 pKa = 10.82 DD375 pKa = 3.86 LTQLKK380 pKa = 9.95 SVSNFDD386 pKa = 3.45 VCDD389 pKa = 3.49 TGVSIAATDD398 pKa = 3.43 ASLPFTAATDD408 pKa = 3.25 IEE410 pKa = 4.96 MYY412 pKa = 10.41 EE413 pKa = 4.22 GMLITFPQEE422 pKa = 3.57 LTVTEE427 pKa = 4.77 TYY429 pKa = 11.03 SLGRR433 pKa = 11.84 YY434 pKa = 7.7 GQVALSSSGKK444 pKa = 9.93 RR445 pKa = 11.84 PNPTQVAAPGEE456 pKa = 4.22 DD457 pKa = 3.24 AVAQAAANALNLIIMDD473 pKa = 4.99 DD474 pKa = 3.6 GSNAQNATISYY485 pKa = 9.11 PGFGEE490 pKa = 3.98 LTYY493 pKa = 11.4 DD494 pKa = 3.3 NAIRR498 pKa = 11.84 GGNTITGLTGVVNYY512 pKa = 7.84 TFSTYY517 pKa = 11.0 SVVPTADD524 pKa = 3.07 VAFEE528 pKa = 4.8 DD529 pKa = 4.59 DD530 pKa = 3.76 NARR533 pKa = 11.84 TATPEE538 pKa = 3.97 DD539 pKa = 3.29 VGGEE543 pKa = 4.04 LKK545 pKa = 11.1 VSGFNVLNYY554 pKa = 7.5 FTTLTSAGSEE564 pKa = 3.95 ICGSGDD570 pKa = 3.42 NYY572 pKa = 10.79 CRR574 pKa = 11.84 GADD577 pKa = 3.45 DD578 pKa = 3.65 AAEE581 pKa = 4.28 FEE583 pKa = 4.33 RR584 pKa = 11.84 QNAKK588 pKa = 10.46 LMSALSAIDD597 pKa = 3.78 ADD599 pKa = 3.48 IFGLIEE605 pKa = 3.97 IEE607 pKa = 4.46 NNAEE611 pKa = 3.84 DD612 pKa = 4.09 TAVSTLVEE620 pKa = 4.14 NLNGEE625 pKa = 4.39 VGADD629 pKa = 3.26 TYY631 pKa = 11.27 SYY633 pKa = 11.7 VMTGMIGEE641 pKa = 4.58 DD642 pKa = 3.76 SIKK645 pKa = 10.29 QALIYY650 pKa = 10.39 KK651 pKa = 7.49 PAKK654 pKa = 10.0 VEE656 pKa = 3.9 PFGDD660 pKa = 4.37 FSIVDD665 pKa = 3.72 YY666 pKa = 11.04 NDD668 pKa = 3.2 SKK670 pKa = 11.44 NRR672 pKa = 11.84 PSLIQTFKK680 pKa = 10.45 TVGGEE685 pKa = 4.08 DD686 pKa = 3.34 EE687 pKa = 5.0 DD688 pKa = 4.73 AFTVVVNHH696 pKa = 6.68 LKK698 pKa = 10.86 SKK700 pKa = 10.87 GSDD703 pKa = 3.41 CDD705 pKa = 3.58 EE706 pKa = 5.42 LGDD709 pKa = 4.67 PDD711 pKa = 6.07 LEE713 pKa = 5.97 DD714 pKa = 4.11 GQGNCNLTRR723 pKa = 11.84 LGAVEE728 pKa = 4.12 TLLTSLNSMDD738 pKa = 3.79 NQNILLLGDD747 pKa = 4.05 FNSYY751 pKa = 11.07 AMEE754 pKa = 4.68 DD755 pKa = 4.33 PITALKK761 pKa = 10.36 DD762 pKa = 3.13 AGYY765 pKa = 10.93 VDD767 pKa = 5.14 LGAEE771 pKa = 4.09 AGEE774 pKa = 4.15 TASYY778 pKa = 11.17 VFDD781 pKa = 4.79 GEE783 pKa = 4.34 WGTLDD788 pKa = 3.49 YY789 pKa = 11.51 AFASASLADD798 pKa = 4.05 KK799 pKa = 11.24 VSDD802 pKa = 3.37 TTEE805 pKa = 3.18 WHH807 pKa = 6.53 INADD811 pKa = 3.74 EE812 pKa = 5.29 PISFDD817 pKa = 3.58 YY818 pKa = 9.86 NTDD821 pKa = 3.77 FKK823 pKa = 11.45 ADD825 pKa = 3.72 TEE827 pKa = 4.19 IASYY831 pKa = 10.87 YY832 pKa = 10.21 GDD834 pKa = 3.47 SAYY837 pKa = 10.4 RR838 pKa = 11.84 SSDD841 pKa = 3.32 HH842 pKa = 7.34 DD843 pKa = 3.84 PVIVGLNITPVVDD856 pKa = 5.14 DD857 pKa = 5.14 GSNDD861 pKa = 4.84 DD862 pKa = 6.09 DD863 pKa = 7.04 DD864 pKa = 7.25 DD865 pKa = 7.33 DD866 pKa = 6.58 DD867 pKa = 5.6 DD868 pKa = 6.0 SSVGGGLAMSPLFMLFSLLGFAAIRR893 pKa = 11.84 LRR895 pKa = 11.84 RR896 pKa = 11.84 KK897 pKa = 9.61
Molecular weight: 94.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.617
IPC_protein 3.668
Toseland 3.439
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.49
Grimsley 3.338
Solomon 3.668
Lehninger 3.617
Nozaki 3.77
DTASelect 4.05
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.795
Patrickios 1.151
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|A0A317CFU3|A0A317CFU3_9GAMM Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase OS=Leucothrix arctica OX=1481894 GN=cobT PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.08 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.95 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTVGGRR28 pKa = 11.84 KK29 pKa = 9.0 VIKK32 pKa = 10.13 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 ALLCPP44 pKa = 4.17
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.377
IPC2_protein 10.804
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.281
Grimsley 12.588
Solomon 13.042
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.018
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4192
0
4192
1404415
18
6951
335.0
37.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.588 ± 0.047
0.96 ± 0.014
5.872 ± 0.043
6.115 ± 0.038
4.022 ± 0.031
6.937 ± 0.04
2.076 ± 0.022
6.343 ± 0.031
5.406 ± 0.041
10.195 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.518 ± 0.024
4.234 ± 0.029
3.964 ± 0.025
3.951 ± 0.032
4.486 ± 0.036
7.1 ± 0.042
5.88 ± 0.067
7.031 ± 0.035
1.262 ± 0.014
3.063 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here