Arthrobacter phage Corgi
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2KF04|A0A3G2KF04_9CAUD Head-to-tail adaptor OS=Arthrobacter phage Corgi OX=2419952 GN=8 PE=4 SV=1
MM1 pKa = 7.83 PATTNRR7 pKa = 11.84 DD8 pKa = 2.95 YY9 pKa = 10.59 RR10 pKa = 11.84 YY11 pKa = 9.02 PGAGVEE17 pKa = 4.11 PDD19 pKa = 3.5 VNGDD23 pKa = 3.39 IQRR26 pKa = 11.84 LAEE29 pKa = 4.86 DD30 pKa = 3.51 VDD32 pKa = 4.32 ADD34 pKa = 3.64 MDD36 pKa = 3.8 ATLAALAKK44 pKa = 10.41 LPGRR48 pKa = 11.84 IQTGSATISIGSAVASATQVITFPVAFASAPNVFLQSTANVAGRR92 pKa = 11.84 ASLLSLYY99 pKa = 10.96 VNATTATQFTCKK111 pKa = 9.47 MQTADD116 pKa = 3.58 NANTGTSYY124 pKa = 11.24 AIAYY128 pKa = 8.62 NWIAVLL134 pKa = 4.17
Molecular weight: 13.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.417
IPC2_protein 4.546
IPC_protein 4.368
Toseland 4.151
ProMoST 4.546
Dawson 4.368
Bjellqvist 4.533
Wikipedia 4.342
Rodwell 4.202
Grimsley 4.062
Solomon 4.355
Lehninger 4.317
Nozaki 4.495
DTASelect 4.774
Thurlkill 4.228
EMBOSS 4.342
Sillero 4.495
Patrickios 3.541
IPC_peptide 4.355
IPC2_peptide 4.469
IPC2.peptide.svr19 4.463
Protein with the highest isoelectric point:
>tr|A0A3G2KF60|A0A3G2KF60_9CAUD Membrane protein OS=Arthrobacter phage Corgi OX=2419952 GN=17 PE=4 SV=1
MM1 pKa = 7.68 ARR3 pKa = 11.84 RR4 pKa = 11.84 PAPPQGQLFRR14 pKa = 11.84 MDD16 pKa = 4.01 PVQVPGQDD24 pKa = 2.89 GHH26 pKa = 6.73 RR27 pKa = 11.84 AKK29 pKa = 10.51 KK30 pKa = 8.42 WGGRR34 pKa = 11.84 KK35 pKa = 7.96 VAKK38 pKa = 10.56 ARR40 pKa = 11.84 DD41 pKa = 3.79 TVRR44 pKa = 11.84 AMLPAACWRR53 pKa = 11.84 GCGRR57 pKa = 11.84 MITRR61 pKa = 11.84 DD62 pKa = 3.3 WPEE65 pKa = 4.02 KK66 pKa = 9.72 DD67 pKa = 2.53 WHH69 pKa = 6.42 AGHH72 pKa = 6.43 VNGRR76 pKa = 11.84 AEE78 pKa = 4.35 GGPDD82 pKa = 3.01 NVTNYY87 pKa = 10.32 LPEE90 pKa = 4.46 CRR92 pKa = 11.84 WCNLSEE98 pKa = 4.2 GGKK101 pKa = 10.04 VGAAITNGKK110 pKa = 9.1 RR111 pKa = 11.84 VAITTDD117 pKa = 4.13 AIRR120 pKa = 11.84 EE121 pKa = 4.15 RR122 pKa = 11.84 TLKK125 pKa = 10.0 WYY127 pKa = 10.34
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 9.341
IPC_protein 9.56
Toseland 10.423
ProMoST 10.014
Dawson 10.526
Bjellqvist 10.175
Wikipedia 10.657
Rodwell 10.862
Grimsley 10.555
Solomon 10.584
Lehninger 10.57
Nozaki 10.452
DTASelect 10.16
Thurlkill 10.423
EMBOSS 10.818
Sillero 10.452
Patrickios 10.613
IPC_peptide 10.599
IPC2_peptide 9.238
IPC2.peptide.svr19 8.332
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26
0
26
5037
48
555
193.7
20.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.989 ± 0.704
0.596 ± 0.174
6.79 ± 0.471
4.963 ± 0.448
2.104 ± 0.162
8.795 ± 0.335
1.37 ± 0.19
3.176 ± 0.304
3.633 ± 0.464
8.537 ± 0.589
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.363 ± 0.19
2.918 ± 0.308
5.122 ± 0.523
3.871 ± 0.324
6.75 ± 0.51
4.864 ± 0.43
7.227 ± 0.415
7.504 ± 0.547
2.005 ± 0.254
2.422 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here