Lone star tick rhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Lostrhavirus; Lonestar lostrhavirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7TF62|A0A1N7TF62_9RHAB Phosphoprotein OS=Lone star tick rhabdovirus OX=1756186 PE=4 SV=1
MM1 pKa = 7.34EE2 pKa = 5.48LWPVNVEE9 pKa = 4.38KK10 pKa = 10.92INFRR14 pKa = 11.84PIVTPVTTEE23 pKa = 3.71TAPTYY28 pKa = 9.82PSVWFDD34 pKa = 2.69AHH36 pKa = 6.99ANQKK40 pKa = 10.09PGITARR46 pKa = 11.84VGEE49 pKa = 4.49ANLAYY54 pKa = 10.48HH55 pKa = 6.34NMIIMLEE62 pKa = 4.02QQQWTEE68 pKa = 4.09LTVVSYY74 pKa = 8.17MVAVIRR80 pKa = 11.84EE81 pKa = 4.06NPNIFSTVPTAAWAANGRR99 pKa = 11.84PVARR103 pKa = 11.84ANQRR107 pKa = 11.84VSVLDD112 pKa = 3.36ILTVTNGAAWAAVQQARR129 pKa = 11.84ASTRR133 pKa = 11.84EE134 pKa = 3.89SRR136 pKa = 11.84LSLFWATLLLYY147 pKa = 10.41RR148 pKa = 11.84KK149 pKa = 7.29TLARR153 pKa = 11.84EE154 pKa = 3.65VDD156 pKa = 3.21RR157 pKa = 11.84EE158 pKa = 4.15YY159 pKa = 11.46GNKK162 pKa = 9.4IARR165 pKa = 11.84QLNDD169 pKa = 3.21FFKK172 pKa = 11.12GGVLQMDD179 pKa = 3.45VDD181 pKa = 4.07DD182 pKa = 4.59TEE184 pKa = 4.56EE185 pKa = 5.02DD186 pKa = 3.75GLKK189 pKa = 10.53LGKK192 pKa = 10.14DD193 pKa = 3.43KK194 pKa = 11.61LKK196 pKa = 10.92DD197 pKa = 3.59GYY199 pKa = 10.65LKK201 pKa = 10.76LVAAIDD207 pKa = 3.63MFFFKK212 pKa = 11.01YY213 pKa = 9.53QDD215 pKa = 3.44HH216 pKa = 6.98EE217 pKa = 4.53YY218 pKa = 11.43SMVRR222 pKa = 11.84MATLPSRR229 pKa = 11.84FKK231 pKa = 10.54GCAAITSAGQVAKK244 pKa = 10.51ILGIEE249 pKa = 4.09PGEE252 pKa = 4.34LGLFVYY258 pKa = 8.35TEE260 pKa = 4.1PVAVEE265 pKa = 4.19LANSNRR271 pKa = 11.84PNQGMEE277 pKa = 3.94DD278 pKa = 3.7LYY280 pKa = 11.23GYY282 pKa = 10.19FPYY285 pKa = 10.75QMDD288 pKa = 2.86MGMTQRR294 pKa = 11.84SAYY297 pKa = 9.95SISSNPTLYY306 pKa = 10.8LWLHH310 pKa = 6.47TIGCTVGFQRR320 pKa = 11.84SFQARR325 pKa = 11.84YY326 pKa = 8.92PEE328 pKa = 4.12AAGDD332 pKa = 3.53ISGTMISAAAVGLAFSKK349 pKa = 10.02GLKK352 pKa = 8.53MRR354 pKa = 11.84RR355 pKa = 11.84QFGTTEE361 pKa = 3.62EE362 pKa = 4.04AANALRR368 pKa = 11.84ARR370 pKa = 11.84NQAIDD375 pKa = 3.57QAEE378 pKa = 3.84AAAAEE383 pKa = 4.56VGEE386 pKa = 4.65EE387 pKa = 4.45APPPPPPAQEE397 pKa = 4.02GVEE400 pKa = 4.06AVADD404 pKa = 4.25GGDD407 pKa = 3.88GEE409 pKa = 4.85GDD411 pKa = 3.31AAALDD416 pKa = 4.13IPPPPAEE423 pKa = 4.42EE424 pKa = 4.2NPLAAMSNTILDD436 pKa = 3.75NLIEE440 pKa = 4.38NEE442 pKa = 4.22GLTKK446 pKa = 10.91DD447 pKa = 3.2EE448 pKa = 4.11MKK450 pKa = 10.45EE451 pKa = 3.73FRR453 pKa = 11.84RR454 pKa = 11.84RR455 pKa = 11.84LILGRR460 pKa = 11.84TLRR463 pKa = 11.84EE464 pKa = 3.93TTVGRR469 pKa = 11.84RR470 pKa = 11.84LANIYY475 pKa = 10.77NPGAA479 pKa = 3.62

Molecular weight: 52.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7TF64|A0A1N7TF64_9RHAB Glycoprotein OS=Lone star tick rhabdovirus OX=1756186 PE=3 SV=1
MM1 pKa = 7.7KK2 pKa = 9.22KK3 pKa = 6.64TTNLIHH9 pKa = 7.17IEE11 pKa = 4.06LDD13 pKa = 3.28SLRR16 pKa = 11.84VVSVIRR22 pKa = 11.84SIMLRR27 pKa = 11.84PIRR30 pKa = 11.84DD31 pKa = 2.91AWKK34 pKa = 10.12KK35 pKa = 9.64IKK37 pKa = 10.77KK38 pKa = 9.53NDD40 pKa = 3.53EE41 pKa = 4.02GTTAPAPSAPSTSQTLTMEE60 pKa = 4.32HH61 pKa = 7.46PYY63 pKa = 11.11LEE65 pKa = 4.59GVTVNVPPPLNPGRR79 pKa = 11.84VITDD83 pKa = 4.03LNLEE87 pKa = 4.6LDD89 pKa = 4.13FKK91 pKa = 11.29MEE93 pKa = 4.02VVTNKK98 pKa = 9.64VEE100 pKa = 3.95TDD102 pKa = 3.62LARR105 pKa = 11.84ISAPLINLQWDD116 pKa = 3.74YY117 pKa = 11.75KK118 pKa = 11.28GDD120 pKa = 3.68LRR122 pKa = 11.84YY123 pKa = 10.63KK124 pKa = 9.37NIWMAFAMVSAFGLEE139 pKa = 4.44GGDD142 pKa = 3.66PQPYY146 pKa = 8.15GCLYY150 pKa = 10.72KK151 pKa = 9.98MEE153 pKa = 4.34ICRR156 pKa = 11.84GIKK159 pKa = 9.83IKK161 pKa = 10.97VEE163 pKa = 3.84SSRR166 pKa = 11.84LFEE169 pKa = 4.22TPRR172 pKa = 11.84EE173 pKa = 4.35FKK175 pKa = 10.01WNQYY179 pKa = 7.11VHH181 pKa = 6.85WLADD185 pKa = 4.59GIHH188 pKa = 6.03SEE190 pKa = 3.92WTFRR194 pKa = 11.84GQAIRR199 pKa = 11.84TCVNYY204 pKa = 10.12KK205 pKa = 10.21AQEE208 pKa = 3.98AKK210 pKa = 10.47KK211 pKa = 10.8GLEE214 pKa = 3.76DD215 pKa = 3.4WLNSMGVPARR225 pKa = 11.84RR226 pKa = 11.84DD227 pKa = 3.67EE228 pKa = 4.22NKK230 pKa = 10.42HH231 pKa = 6.35LILIIDD237 pKa = 3.74QEE239 pKa = 4.25QQ240 pKa = 2.9

Molecular weight: 27.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3742

240

2195

748.4

85.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.864 ± 1.406

1.523 ± 0.327

5.425 ± 0.475

6.36 ± 0.246

3.661 ± 0.392

5.559 ± 0.309

2.539 ± 0.399

7.376 ± 0.562

6.601 ± 0.629

9.594 ± 0.797

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.292

4.703 ± 0.309

5.158 ± 0.636

3.447 ± 0.233

5.104 ± 0.352

7.402 ± 0.663

7.429 ± 0.527

5.131 ± 0.407

1.871 ± 0.259

3.902 ± 0.477
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski