Brochothrix phage NF5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7RWG5|D7RWG5_9CAUD Gp41 OS=Brochothrix phage NF5 OX=764561 PE=4 SV=1
MM1 pKa = 7.88EE2 pKa = 6.72DD3 pKa = 3.67YY4 pKa = 10.79DD5 pKa = 4.04CPKK8 pKa = 10.45CFEE11 pKa = 5.15CGDD14 pKa = 4.06EE15 pKa = 4.32CSMIDD20 pKa = 3.63GLLVCDD26 pKa = 4.66EE27 pKa = 4.25CTKK30 pKa = 10.61TYY32 pKa = 8.51TQQEE36 pKa = 4.19WEE38 pKa = 4.07QANEE42 pKa = 4.03KK43 pKa = 10.13EE44 pKa = 4.29LEE46 pKa = 3.98AFIAFLKK53 pKa = 10.27EE54 pKa = 3.79KK55 pKa = 10.45EE56 pKa = 4.37SAQGG60 pKa = 3.21

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7RWH9|D7RWH9_9CAUD Gp53 OS=Brochothrix phage NF5 OX=764561 PE=4 SV=1
MM1 pKa = 7.19QSFVSIDD8 pKa = 3.54LKK10 pKa = 10.48QQLSIFKK17 pKa = 10.46HH18 pKa = 5.43SLICNRR24 pKa = 11.84KK25 pKa = 9.13AYY27 pKa = 10.65LLLKK31 pKa = 9.8VCFFVGIFIRR41 pKa = 11.84GRR43 pKa = 3.3

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12189

43

915

210.2

23.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.637 ± 0.376

0.755 ± 0.124

6.563 ± 0.198

7.384 ± 0.461

4.283 ± 0.239

6.202 ± 0.552

1.477 ± 0.183

7.039 ± 0.283

9.303 ± 0.346

7.827 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.175

5.694 ± 0.224

2.609 ± 0.217

3.774 ± 0.201

3.823 ± 0.24

6.194 ± 0.275

6.506 ± 0.357

6.727 ± 0.26

1.05 ± 0.118

3.766 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski