Common vole polyomavirus
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0I7J3|A0A0P0I7J3_9POLY Major capsid protein VP1 OS=Common vole polyomavirus OX=1737523 PE=3 SV=1
MM1 pKa = 7.61 GIVLALPEE9 pKa = 4.66 FIAAAVAGGAEE20 pKa = 4.11 ALEE23 pKa = 4.37 IAGSVGALVEE33 pKa = 4.49 GAADD37 pKa = 3.39 TAAAIGTTAEE47 pKa = 4.22 VLGEE51 pKa = 4.15 SLPVTISEE59 pKa = 4.21 AAATVLAQTPEE70 pKa = 4.05 VVEE73 pKa = 4.06 ALNAGGQAVGSAVSLGSGLYY93 pKa = 9.21 QAISNTDD100 pKa = 3.61 PQSGTHH106 pKa = 6.94 GISDD110 pKa = 4.72 RR111 pKa = 11.84 IGMANALVPYY121 pKa = 9.38 QQVPTYY127 pKa = 9.71 LQWLRR132 pKa = 11.84 NILSYY137 pKa = 10.76 IPTVQQIWGGLEE149 pKa = 3.85 RR150 pKa = 11.84 ATQTYY155 pKa = 10.19 QYY157 pKa = 10.95 LDD159 pKa = 3.2 EE160 pKa = 4.24 QYY162 pKa = 11.19 RR163 pKa = 11.84 LRR165 pKa = 11.84 EE166 pKa = 3.84 QQYY169 pKa = 9.13 EE170 pKa = 3.98 RR171 pKa = 11.84 LIAEE175 pKa = 4.61 FEE177 pKa = 4.23 RR178 pKa = 11.84 GVNSGPVAGVIEE190 pKa = 5.2 RR191 pKa = 11.84 IRR193 pKa = 11.84 ATALEE198 pKa = 4.4 RR199 pKa = 11.84 EE200 pKa = 4.57 LEE202 pKa = 4.28 VQRR205 pKa = 11.84 IAGDD209 pKa = 3.51 VRR211 pKa = 11.84 DD212 pKa = 3.46 VSTNLRR218 pKa = 11.84 EE219 pKa = 4.19 GLSASGRR226 pKa = 11.84 AVANVGSALYY236 pKa = 8.68 NTVSGAASATRR247 pKa = 11.84 AAAGTLGGTVSNVVGHH263 pKa = 5.12 VQGTISTIQDD273 pKa = 3.48 YY274 pKa = 11.11 LRR276 pKa = 11.84 TIGSEE281 pKa = 3.71 ASGVSTTMLTDD292 pKa = 4.77 GYY294 pKa = 10.95 NAMPRR299 pKa = 11.84 GISNFGSWVFMSGSDD314 pKa = 3.28 GGTPHH319 pKa = 6.94 YY320 pKa = 11.02 SLDD323 pKa = 3.45 YY324 pKa = 8.27 WVVYY328 pKa = 10.47 ALEE331 pKa = 4.31 EE332 pKa = 4.02 ALKK335 pKa = 10.26 PLYY338 pKa = 9.31 STQNAPVEE346 pKa = 4.47 STVEE350 pKa = 4.07 DD351 pKa = 3.92 EE352 pKa = 6.09 DD353 pKa = 4.28 DD354 pKa = 4.03 TEE356 pKa = 4.35 HH357 pKa = 7.11 TPTKK361 pKa = 10.2 NNKK364 pKa = 7.88 KK365 pKa = 8.95 RR366 pKa = 11.84 RR367 pKa = 11.84 RR368 pKa = 11.84 RR369 pKa = 11.84 SVVSRR374 pKa = 11.84 PSKK377 pKa = 9.43 RR378 pKa = 11.84 RR379 pKa = 11.84 HH380 pKa = 5.7 RR381 pKa = 11.84 SASS384 pKa = 3.25
Molecular weight: 40.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.046
IPC2_protein 5.118
IPC_protein 5.041
Toseland 4.965
ProMoST 5.143
Dawson 5.003
Bjellqvist 5.13
Wikipedia 4.838
Rodwell 4.927
Grimsley 4.889
Solomon 5.003
Lehninger 4.952
Nozaki 5.118
DTASelect 5.219
Thurlkill 4.952
EMBOSS 4.876
Sillero 5.194
Patrickios 4.012
IPC_peptide 5.016
IPC2_peptide 5.194
IPC2.peptide.svr19 5.176
Protein with the highest isoelectric point:
>tr|A0A0P0HGX9|A0A0P0HGX9_9POLY Large T antigen OS=Common vole polyomavirus OX=1737523 PE=4 SV=1
MM1 pKa = 7.56 ANALVPYY8 pKa = 9.38 QQVPTYY14 pKa = 9.71 LQWLRR19 pKa = 11.84 NILSYY24 pKa = 10.76 IPTVQQIWGGLEE36 pKa = 3.85 RR37 pKa = 11.84 ATQTYY42 pKa = 10.19 QYY44 pKa = 10.95 LDD46 pKa = 3.2 EE47 pKa = 4.24 QYY49 pKa = 11.19 RR50 pKa = 11.84 LRR52 pKa = 11.84 EE53 pKa = 3.84 QQYY56 pKa = 9.13 EE57 pKa = 3.98 RR58 pKa = 11.84 LIAEE62 pKa = 4.61 FEE64 pKa = 4.23 RR65 pKa = 11.84 GVNSGPVAGVIEE77 pKa = 5.2 RR78 pKa = 11.84 IRR80 pKa = 11.84 ATALEE85 pKa = 4.4 RR86 pKa = 11.84 EE87 pKa = 4.57 LEE89 pKa = 4.28 VQRR92 pKa = 11.84 IAGDD96 pKa = 3.51 VRR98 pKa = 11.84 DD99 pKa = 3.46 VSTNLRR105 pKa = 11.84 EE106 pKa = 4.19 GLSASGRR113 pKa = 11.84 AVANVGSALYY123 pKa = 8.68 NTVSGAASATRR134 pKa = 11.84 AAAGTLGGTVSNVVGHH150 pKa = 5.12 VQGTISTIQDD160 pKa = 3.48 YY161 pKa = 11.11 LRR163 pKa = 11.84 TIGSEE168 pKa = 3.71 ASGVSTTMLTDD179 pKa = 4.77 GYY181 pKa = 10.95 NAMPRR186 pKa = 11.84 GISNFGSWVFMSGSDD201 pKa = 3.28 GGTPHH206 pKa = 6.94 YY207 pKa = 11.02 SLDD210 pKa = 3.45 YY211 pKa = 8.27 WVVYY215 pKa = 10.47 ALEE218 pKa = 4.31 EE219 pKa = 4.02 ALKK222 pKa = 10.26 PLYY225 pKa = 9.31 STQNAPVEE233 pKa = 4.47 STVEE237 pKa = 4.07 DD238 pKa = 3.92 EE239 pKa = 6.09 DD240 pKa = 4.28 DD241 pKa = 4.03 TEE243 pKa = 4.35 HH244 pKa = 7.11 TPTKK248 pKa = 10.2 NNKK251 pKa = 7.88 KK252 pKa = 8.95 RR253 pKa = 11.84 RR254 pKa = 11.84 RR255 pKa = 11.84 RR256 pKa = 11.84 SVVSRR261 pKa = 11.84 PSKK264 pKa = 9.43 RR265 pKa = 11.84 RR266 pKa = 11.84 HH267 pKa = 5.7 RR268 pKa = 11.84 SASS271 pKa = 3.25
Molecular weight: 29.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.438
IPC2_protein 7.454
IPC_protein 8.331
Toseland 8.331
ProMoST 7.819
Dawson 8.463
Bjellqvist 7.922
Wikipedia 8.639
Rodwell 8.39
Grimsley 8.404
Solomon 8.843
Lehninger 8.785
Nozaki 7.966
DTASelect 8.317
Thurlkill 8.273
EMBOSS 8.639
Sillero 8.419
Patrickios 4.291
IPC_peptide 8.829
IPC2_peptide 7.468
IPC2.peptide.svr19 8.004
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1844
189
646
368.8
41.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.941 ± 1.522
2.223 ± 0.95
4.284 ± 0.527
7.646 ± 0.37
3.416 ± 0.852
6.941 ± 1.106
2.115 ± 0.558
4.826 ± 0.23
4.61 ± 1.355
8.785 ± 0.787
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.44 ± 0.411
5.043 ± 0.41
4.555 ± 0.84
4.393 ± 0.196
6.345 ± 0.589
6.996 ± 0.667
5.911 ± 0.982
7.05 ± 1.081
1.735 ± 0.379
3.742 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here