Ranid herpesvirus 3
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 186 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X9T5L2|A0A1X9T5L2_9VIRU C-type lectin protein OS=Ranid herpesvirus 3 OX=1987509 PE=4 SV=1
MM1 pKa = 7.39 SFAVDD6 pKa = 3.51 DD7 pKa = 4.25 RR8 pKa = 11.84 LVSVVVIVGTAVFTVVMVTVGIGAFVVVVTICVAVALVVTLAFVVAIIVAVALFVTLAFVVAIIVAVEE76 pKa = 3.74 LVITLAFVVAIVALMVVLIAEE97 pKa = 4.68 AVTSVSSVLVDD108 pKa = 3.56 GTTVEE113 pKa = 4.26 GFVMGKK119 pKa = 10.25 GDD121 pKa = 3.79 PTSVQNDD128 pKa = 3.34 EE129 pKa = 4.16 PAFMISKK136 pKa = 10.29
Molecular weight: 13.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.361
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 3.834
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A1X9T569|A0A1X9T569_9VIRU Uncharacterized protein OS=Ranid herpesvirus 3 OX=1987509 PE=4 SV=1
MM1 pKa = 7.21 VSKK4 pKa = 10.69 HH5 pKa = 5.59 PPAYY9 pKa = 9.66 SSGFFEE15 pKa = 4.24 HH16 pKa = 7.45 RR17 pKa = 11.84 AMQSSLHH24 pKa = 5.04 EE25 pKa = 4.3 AKK27 pKa = 10.8 GNVGSRR33 pKa = 11.84 PATPPIAFSPRR44 pKa = 11.84 VGAPHH49 pKa = 6.09 VPSRR53 pKa = 11.84 DD54 pKa = 3.04 GFNQNTHH61 pKa = 6.65 ALGQTQSGFSRR72 pKa = 11.84 QNPGRR77 pKa = 11.84 QPFKK81 pKa = 11.11 SKK83 pKa = 10.28 ISRR86 pKa = 11.84 LQPIYY91 pKa = 9.08 EE92 pKa = 4.29 TQTLHH97 pKa = 7.48 RR98 pKa = 11.84 FVQRR102 pKa = 11.84 EE103 pKa = 3.58 ARR105 pKa = 11.84 RR106 pKa = 11.84 TIEE109 pKa = 4.65 HH110 pKa = 5.95 VGAPNEE116 pKa = 4.04 PVPPGTFVSVSPTARR131 pKa = 11.84 TGQNHH136 pKa = 4.98 SRR138 pKa = 11.84 EE139 pKa = 4.07 NQRR142 pKa = 11.84 NIITTTAGHH151 pKa = 6.73 RR152 pKa = 11.84 NLPTHH157 pKa = 5.58 FVEE160 pKa = 5.11 YY161 pKa = 10.83 SRR163 pKa = 11.84 ANPKK167 pKa = 9.75 NLPDD171 pKa = 5.18 LIPVEE176 pKa = 4.95 RR177 pKa = 11.84 YY178 pKa = 7.4 EE179 pKa = 4.34 FPKK182 pKa = 10.22 SCRR185 pKa = 11.84 GMDD188 pKa = 3.29 SAGRR192 pKa = 11.84 CQRR195 pKa = 11.84 LFSGSFRR202 pKa = 11.84 IFTGDD207 pKa = 3.22 LYY209 pKa = 11.39 EE210 pKa = 4.87 DD211 pKa = 4.02 GKK213 pKa = 11.08 RR214 pKa = 11.84 EE215 pKa = 4.02 NIVII219 pKa = 4.11
Molecular weight: 24.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.589
IPC_protein 10.452
Toseland 10.526
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 10.804
Grimsley 10.716
Solomon 10.774
Lehninger 10.73
Nozaki 10.526
DTASelect 10.394
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.584
Patrickios 10.496
IPC_peptide 10.774
IPC2_peptide 9.428
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
186
0
186
67043
89
4633
360.4
40.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.311 ± 0.116
2.822 ± 0.147
4.8 ± 0.154
5.431 ± 0.221
4.627 ± 0.146
4.596 ± 0.129
2.543 ± 0.096
5.869 ± 0.089
5.743 ± 0.153
9.856 ± 0.138
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.077
5.565 ± 0.138
4.785 ± 0.155
3.459 ± 0.126
4.992 ± 0.136
7.54 ± 0.147
6.663 ± 0.179
6.409 ± 0.119
1.247 ± 0.087
4.236 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here