Pseudomonas phage PA10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pakpunavirus; Pseudomonas virus PA10

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MI46|A0A1J0MI46_9CAUD Uncharacterized protein OS=Pseudomonas phage PA10 OX=1913575 PE=4 SV=1
MM1 pKa = 7.17TVYY4 pKa = 10.7KK5 pKa = 9.65FWCEE9 pKa = 2.9WDD11 pKa = 2.89IGINEE16 pKa = 4.26FLWRR20 pKa = 11.84DD21 pKa = 3.72YY22 pKa = 11.34YY23 pKa = 11.62QMEE26 pKa = 4.3EE27 pKa = 4.29DD28 pKa = 3.65VAKK31 pKa = 10.66ALSDD35 pKa = 3.75CGIEE39 pKa = 4.0DD40 pKa = 4.09TIEE43 pKa = 4.01EE44 pKa = 4.43LEE46 pKa = 4.31GAGLLGFDD54 pKa = 3.72SVKK57 pKa = 10.98VIGG60 pKa = 4.11

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MI67|A0A1J0MI67_9CAUD Uncharacterized protein OS=Pseudomonas phage PA10 OX=1913575 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.22RR3 pKa = 11.84QYY5 pKa = 10.12TDD7 pKa = 3.11SYY9 pKa = 9.11QCKK12 pKa = 10.24AIDD15 pKa = 4.02GLMSICGGKK24 pKa = 9.6KK25 pKa = 10.25AIVQVWPSLKK35 pKa = 10.09ASRR38 pKa = 11.84NRR40 pKa = 11.84RR41 pKa = 11.84LKK43 pKa = 11.17KK44 pKa = 8.32MIKK47 pKa = 9.82YY48 pKa = 9.43IARR51 pKa = 11.84SGWRR55 pKa = 11.84PP56 pKa = 2.99

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

21903

37

904

172.5

19.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.967 ± 0.319

1.292 ± 0.112

6.364 ± 0.159

7.876 ± 0.307

4.2 ± 0.143

7.072 ± 0.257

1.927 ± 0.129

5.41 ± 0.189

6.469 ± 0.245

7.766 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.022 ± 0.118

4.246 ± 0.181

3.607 ± 0.166

3.557 ± 0.142

5.419 ± 0.201

5.73 ± 0.203

5.447 ± 0.229

7.273 ± 0.245

1.689 ± 0.111

3.666 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski