Streptococcus henryi
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2315 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6BDD2|A0A1G6BDD2_9STRE GyrI-like domain-containing protein OS=Streptococcus henryi OX=439219 GN=SAMN02910293_00929 PE=4 SV=1
MM1 pKa = 7.42 IKK3 pKa = 10.17 VKK5 pKa = 10.45 KK6 pKa = 10.59 VLVLLFSVFLLASCSSNKK24 pKa = 9.86 SSEE27 pKa = 3.91 NAIDD31 pKa = 3.49 EE32 pKa = 4.48 GQIEE36 pKa = 4.85 LIKK39 pKa = 10.66 DD40 pKa = 3.45 VQASVSEE47 pKa = 4.01 NFYY50 pKa = 10.41 YY51 pKa = 10.37 QQLSDD56 pKa = 3.92 VEE58 pKa = 4.32 KK59 pKa = 10.73 EE60 pKa = 3.7 NYY62 pKa = 9.63 ARR64 pKa = 11.84 LRR66 pKa = 11.84 LAFNDD71 pKa = 4.13 FSDD74 pKa = 3.73 EE75 pKa = 4.18 VTLKK79 pKa = 10.81 EE80 pKa = 4.46 VDD82 pKa = 3.98 DD83 pKa = 4.51 EE84 pKa = 4.58 SLAKK88 pKa = 10.35 ISTAFTNDD96 pKa = 3.14 NPDD99 pKa = 3.39 VFWFNDD105 pKa = 3.14 YY106 pKa = 10.2 TYY108 pKa = 9.72 QTDD111 pKa = 4.21 GNDD114 pKa = 3.38 QLILEE119 pKa = 5.0 IEE121 pKa = 4.33 TPDD124 pKa = 3.67 DD125 pKa = 3.55 VEE127 pKa = 5.02 EE128 pKa = 4.49 IYY130 pKa = 10.9 QLIEE134 pKa = 4.05 VMSEE138 pKa = 3.81 QILAEE143 pKa = 4.26 MPTDD147 pKa = 3.37 SDD149 pKa = 4.01 YY150 pKa = 11.94 SRR152 pKa = 11.84 VKK154 pKa = 10.5 YY155 pKa = 10.35 LYY157 pKa = 10.3 EE158 pKa = 4.31 FIINYY163 pKa = 8.7 TDD165 pKa = 3.65 YY166 pKa = 10.12 NTLALTDD173 pKa = 4.42 DD174 pKa = 4.14 SEE176 pKa = 4.53 LDD178 pKa = 3.08 KK179 pKa = 11.65 GQDD182 pKa = 3.05 IRR184 pKa = 11.84 SVFIDD189 pKa = 3.46 QLSVCSGYY197 pKa = 11.21 SKK199 pKa = 10.95 AFQYY203 pKa = 10.54 LANKK207 pKa = 10.0 AGIPTTYY214 pKa = 9.67 IVGFARR220 pKa = 11.84 SDD222 pKa = 3.27 KK223 pKa = 10.44 TEE225 pKa = 3.97 EE226 pKa = 4.27 SEE228 pKa = 6.38 LPHH231 pKa = 6.49 AWNTVIINGQSYY243 pKa = 10.57 NVDD246 pKa = 3.85 TTWGDD251 pKa = 3.72 PIFEE255 pKa = 4.7 GEE257 pKa = 4.1 MSEE260 pKa = 5.74 DD261 pKa = 3.28 MSDD264 pKa = 3.58 TINYY268 pKa = 8.8 HH269 pKa = 5.76 FLCVPDD275 pKa = 3.77 YY276 pKa = 11.27 LFNNSHH282 pKa = 5.95 TASDD286 pKa = 5.13 DD287 pKa = 3.33 ILFQDD292 pKa = 4.78 GSSIDD297 pKa = 4.47 DD298 pKa = 3.45 VWTLPTSTDD307 pKa = 2.83 NSLNYY312 pKa = 9.67 YY313 pKa = 8.62 VQAGCFFEE321 pKa = 5.05 SYY323 pKa = 9.83 QRR325 pKa = 11.84 GEE327 pKa = 3.9 MEE329 pKa = 4.59 SYY331 pKa = 10.78 VEE333 pKa = 3.94 QLSHH337 pKa = 7.18 SEE339 pKa = 3.79 NLKK342 pKa = 10.17 QIEE345 pKa = 4.32 LVFEE349 pKa = 4.21 NQEE352 pKa = 4.21 SYY354 pKa = 11.66 DD355 pKa = 3.71 LALNDD360 pKa = 4.09 LLEE363 pKa = 4.43 EE364 pKa = 4.56 GFFGEE369 pKa = 5.72 LIQKK373 pKa = 9.86 YY374 pKa = 9.83 ILGDD378 pKa = 2.96 IDD380 pKa = 3.9 FEE382 pKa = 5.43 YY383 pKa = 11.31 NFIYY387 pKa = 10.69 DD388 pKa = 3.55 DD389 pKa = 3.73 VNYY392 pKa = 9.2 YY393 pKa = 9.98 ICINIPGRR401 pKa = 3.61
Molecular weight: 46.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.605
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.935
Patrickios 0.477
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A1G6D2V2|A0A1G6D2V2_9STRE Transposase IS200 like (Fragment) OS=Streptococcus henryi OX=439219 GN=SAMN02910293_01943 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2315
0
2315
692190
15
2261
299.0
33.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.503 ± 0.062
0.531 ± 0.012
6.059 ± 0.048
6.989 ± 0.055
4.615 ± 0.045
6.483 ± 0.046
1.77 ± 0.023
7.299 ± 0.053
6.789 ± 0.046
9.848 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.481 ± 0.028
4.745 ± 0.04
3.155 ± 0.028
3.917 ± 0.033
3.853 ± 0.037
6.486 ± 0.066
5.79 ± 0.041
6.877 ± 0.043
0.907 ± 0.017
3.904 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here