Alicyclobacillus macrosporangiidus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Alicyclobacillus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7KM73|A0A1I7KM73_9BACL Uncharacterized protein OS=Alicyclobacillus macrosporangiidus OX=392015 GN=SAMN05421543_11728 PE=4 SV=1
MM1 pKa = 7.31AWDD4 pKa = 3.15WMLIEE9 pKa = 4.69VCDD12 pKa = 4.9ANPADD17 pKa = 4.3CPEE20 pKa = 4.28LFALEE25 pKa = 4.14QEE27 pKa = 4.56FPGLSVLEE35 pKa = 4.3TACMSHH41 pKa = 7.32CDD43 pKa = 3.6LCASSPYY50 pKa = 10.59VLLDD54 pKa = 3.38GEE56 pKa = 4.91MVTAPDD62 pKa = 3.43TTALFDD68 pKa = 3.76KK69 pKa = 10.75VRR71 pKa = 11.84EE72 pKa = 3.99EE73 pKa = 4.0LARR76 pKa = 11.84RR77 pKa = 11.84MADD80 pKa = 3.09QRR82 pKa = 11.84SEE84 pKa = 3.75II85 pKa = 4.01

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7LLG9|A0A1I7LLG9_9BACL Uncharacterized protein (Fragment) OS=Alicyclobacillus macrosporangiidus OX=392015 GN=SAMN05421543_1921 PE=4 SV=1
MM1 pKa = 7.31RR2 pKa = 11.84RR3 pKa = 11.84IVRR6 pKa = 11.84RR7 pKa = 11.84VAIRR11 pKa = 11.84DD12 pKa = 3.68PVWRR16 pKa = 11.84GRR18 pKa = 11.84MIRR21 pKa = 11.84RR22 pKa = 11.84WLRR25 pKa = 11.84LRR27 pKa = 11.84QAGSGMSAMMSNLIWLLATITLSTAGGLLLGNTLLNGVFPAALNAFTAMFGAA79 pKa = 4.83

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3795

0

3795

1092489

29

1955

287.9

31.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.038 ± 0.055

0.941 ± 0.015

5.159 ± 0.031

5.923 ± 0.045

3.455 ± 0.027

8.357 ± 0.042

2.412 ± 0.018

4.706 ± 0.033

2.944 ± 0.03

10.101 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.021

2.44 ± 0.027

5.171 ± 0.034

3.831 ± 0.034

7.608 ± 0.048

4.936 ± 0.033

5.293 ± 0.038

8.834 ± 0.037

1.673 ± 0.021

2.716 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski