Lake Sarah-associated circular virus-15

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA72|A0A126GA72_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-15 OX=1685741 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 6.98PITDD6 pKa = 3.67TTIKK10 pKa = 7.83STRR13 pKa = 11.84WAFTAYY19 pKa = 9.67EE20 pKa = 4.41GQWHH24 pKa = 7.02LFEE27 pKa = 4.66TMPPEE32 pKa = 3.72IAEE35 pKa = 4.32WGWQTEE41 pKa = 4.27KK42 pKa = 11.28CPDD45 pKa = 3.7TDD47 pKa = 3.37RR48 pKa = 11.84LHH50 pKa = 5.34YY51 pKa = 10.03QGYY54 pKa = 10.89LMLQRR59 pKa = 11.84QQRR62 pKa = 11.84LASLVRR68 pKa = 11.84LLPGVHH74 pKa = 6.83FGAARR79 pKa = 11.84NWTDD83 pKa = 3.3LVNYY87 pKa = 9.05CRR89 pKa = 11.84KK90 pKa = 9.88KK91 pKa = 8.92DD92 pKa = 3.55TRR94 pKa = 11.84VEE96 pKa = 3.96GTEE99 pKa = 3.81QVHH102 pKa = 4.98RR103 pKa = 11.84TNRR106 pKa = 11.84IPTLFGYY113 pKa = 9.71AQEE116 pKa = 4.53VISRR120 pKa = 11.84LPSWDD125 pKa = 4.69EE126 pKa = 3.88IRR128 pKa = 11.84QDD130 pKa = 3.39WITKK134 pKa = 7.92MEE136 pKa = 4.31HH137 pKa = 5.39VSWMLKK143 pKa = 10.02HH144 pKa = 5.92GQPDD148 pKa = 3.56LPDD151 pKa = 3.96PVPRR155 pKa = 11.84SSIFYY160 pKa = 9.74VATSMEE166 pKa = 3.71AHH168 pKa = 7.0AYY170 pKa = 10.42DD171 pKa = 5.12LVTKK175 pKa = 10.62LVGEE179 pKa = 5.42DD180 pKa = 3.23IQKK183 pKa = 10.45GQIGVEE189 pKa = 4.32FIVQNPLWITTFKK202 pKa = 10.69NQIRR206 pKa = 11.84NMVLRR211 pKa = 11.84KK212 pKa = 10.09DD213 pKa = 3.97FQNPPASRR221 pKa = 11.84QTDD224 pKa = 3.48RR225 pKa = 11.84QTEE228 pKa = 3.93ITISFEE234 pKa = 3.89

Molecular weight: 27.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA72|A0A126GA72_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-15 OX=1685741 PE=4 SV=1
MM1 pKa = 7.59PPRR4 pKa = 11.84RR5 pKa = 11.84VRR7 pKa = 11.84RR8 pKa = 11.84QRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84PARR15 pKa = 11.84KK16 pKa = 8.46YY17 pKa = 7.75RR18 pKa = 11.84QKK20 pKa = 11.02GVRR23 pKa = 11.84TKK25 pKa = 10.64KK26 pKa = 10.18GVKK29 pKa = 9.97ALVTRR34 pKa = 11.84VINAKK39 pKa = 10.62AEE41 pKa = 4.15TKK43 pKa = 9.64MVAFYY48 pKa = 10.48GGPIANPSPLRR59 pKa = 11.84NSTGTYY65 pKa = 10.08ADD67 pKa = 3.96SAPTSQNQYY76 pKa = 9.59ISVNATDD83 pKa = 3.84ILKK86 pKa = 10.18VIPDD90 pKa = 4.0VAPGNADD97 pKa = 3.23NEE99 pKa = 4.38RR100 pKa = 11.84TGRR103 pKa = 11.84YY104 pKa = 9.05INPTSLQLHH113 pKa = 6.32CKK115 pKa = 10.25VGISPISTGGSGYY128 pKa = 10.3QNGWAYY134 pKa = 10.51DD135 pKa = 4.18IIAVAYY141 pKa = 9.2LLQHH145 pKa = 6.08VEE147 pKa = 4.13YY148 pKa = 8.63KK149 pKa = 10.18TYY151 pKa = 10.18QALYY155 pKa = 8.6TNNTFGQLLDD165 pKa = 3.76VMEE168 pKa = 4.65GTTTQFNGDD177 pKa = 3.46FSSANMPVEE186 pKa = 3.72KK187 pKa = 10.21GYY189 pKa = 11.1YY190 pKa = 9.04RR191 pKa = 11.84VLGKK195 pKa = 10.57KK196 pKa = 9.83KK197 pKa = 9.99ISLRR201 pKa = 11.84SSGAYY206 pKa = 9.83NPAAGILNTITNNNSHH222 pKa = 5.79QLSHH226 pKa = 5.89EE227 pKa = 4.2WTWNVGKK234 pKa = 9.66HH235 pKa = 5.06LPKK238 pKa = 10.49KK239 pKa = 10.47LIYY242 pKa = 10.07PEE244 pKa = 4.3GTVSVANGQNEE255 pKa = 4.55PLNAAPFWAVGYY267 pKa = 9.9YY268 pKa = 10.41NVDD271 pKa = 3.2GTGGSPATAKK281 pKa = 10.53INIQQQYY288 pKa = 6.23TAIMKK293 pKa = 10.38FKK295 pKa = 10.97DD296 pKa = 3.51FF297 pKa = 4.15

Molecular weight: 32.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

531

234

297

265.5

30.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.968 ± 1.458

0.565 ± 0.186

4.331 ± 1.062

4.331 ± 1.337

3.013 ± 0.536

6.968 ± 1.458

2.26 ± 0.47

6.026 ± 0.247

5.65 ± 0.885

6.403 ± 0.554

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.47

5.838 ± 1.83

6.026 ± 0.028

6.026 ± 0.796

6.215 ± 0.675

5.273 ± 0.643

8.286 ± 0.442

6.403 ± 0.27

2.448 ± 0.899

4.708 ± 1.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski