Erythrobacter aureus
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3245 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345YI50|A0A345YI50_9SPHN ATP synthase subunit a OS=Erythrobacter aureus OX=2182384 GN=atpB PE=3 SV=1
MM1 pKa = 7.24 MLAACGSDD9 pKa = 3.37 ADD11 pKa = 4.47 EE12 pKa = 5.23 PPTDD16 pKa = 3.93 LEE18 pKa = 4.31 TAEE21 pKa = 4.34 SVGAAEE27 pKa = 3.94 ALARR31 pKa = 11.84 NIEE34 pKa = 4.12 AVDD37 pKa = 4.1 FLDD40 pKa = 5.18 LEE42 pKa = 4.72 LGAKK46 pKa = 9.18 IVGPQGPEE54 pKa = 3.5 VKK56 pKa = 10.29 AALSNPEE63 pKa = 3.93 GNFADD68 pKa = 3.42 MRR70 pKa = 11.84 SYY72 pKa = 10.07 VACPAGMTVCDD83 pKa = 4.21 PATAPEE89 pKa = 4.29 GTIYY93 pKa = 10.22 TYY95 pKa = 10.45 VHH97 pKa = 5.92 IVYY100 pKa = 9.54 PGEE103 pKa = 4.53 DD104 pKa = 3.58 NQPDD108 pKa = 3.74 SGSGEE113 pKa = 4.23 GADD116 pKa = 3.48 SSDD119 pKa = 3.61 VEE121 pKa = 4.13 RR122 pKa = 11.84 ATAFRR127 pKa = 11.84 MTRR130 pKa = 11.84 PSTGFTGAVGYY141 pKa = 10.84 SKK143 pKa = 11.06 DD144 pKa = 3.33 EE145 pKa = 3.87 AMAAIGAKK153 pKa = 9.61 ADD155 pKa = 3.68 VVITCDD161 pKa = 4.25 DD162 pKa = 3.9 GALVWTVSAGDD173 pKa = 5.32 GGDD176 pKa = 2.94 QWEE179 pKa = 4.29 QAEE182 pKa = 4.82 PLTFWWQSTVPPAGPVEE199 pKa = 4.49 AYY201 pKa = 10.19 AIEE204 pKa = 4.49 ANYY207 pKa = 8.79 TMAMGNGPYY216 pKa = 9.94 PGEE219 pKa = 4.42 APDD222 pKa = 5.12 AVNACDD228 pKa = 3.75 RR229 pKa = 11.84 DD230 pKa = 3.9 IVSGAEE236 pKa = 3.99 GG237 pKa = 3.12
Molecular weight: 24.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.706
IPC_protein 3.694
Toseland 3.49
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.726
Protein with the highest isoelectric point:
>tr|A0A345YAL8|A0A345YAL8_9SPHN Two pore domain potassium channel family protein OS=Erythrobacter aureus OX=2182384 GN=DVR09_00270 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.0 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.44 NLCAA44 pKa = 4.54
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3245
0
3245
978490
30
1559
301.5
32.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.25 ± 0.057
0.852 ± 0.012
6.313 ± 0.035
6.605 ± 0.044
3.65 ± 0.032
8.654 ± 0.043
2.012 ± 0.022
5.132 ± 0.029
3.339 ± 0.041
9.613 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.021
2.641 ± 0.025
5.063 ± 0.036
3.107 ± 0.022
7.143 ± 0.044
5.416 ± 0.037
5.16 ± 0.026
6.855 ± 0.031
1.401 ± 0.02
2.268 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here