Thermomonas fusca
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2622 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5R9PIB8|A0A5R9PIB8_9GAMM Ferredoxin--NADP reductase OS=Thermomonas fusca OX=215690 GN=E5S66_01420 PE=3 SV=1
MM1 pKa = 7.97 SEE3 pKa = 3.33 IRR5 pKa = 11.84 IVGKK9 pKa = 9.49 PGKK12 pKa = 8.97 ATRR15 pKa = 11.84 KK16 pKa = 8.75 PRR18 pKa = 11.84 TVKK21 pKa = 10.16 LAPMARR27 pKa = 11.84 AVRR30 pKa = 11.84 SALAVSAMAFALGTTGNVMAGAHH53 pKa = 5.25 EE54 pKa = 4.7 VSTAHH59 pKa = 7.28 ALQIQRR65 pKa = 11.84 AAIDD69 pKa = 3.71 FAPVFDD75 pKa = 4.3 LTVVGAGPVALAIDD89 pKa = 3.78 VTDD92 pKa = 4.73 PGDD95 pKa = 3.56 IVIDD99 pKa = 3.69 NVDD102 pKa = 4.6 PISEE106 pKa = 4.28 YY107 pKa = 11.18 AAGDD111 pKa = 4.26 AIAIRR116 pKa = 11.84 GYY118 pKa = 8.87 STGGNVDD125 pKa = 2.96 ITNAATGVLIADD137 pKa = 4.27 AGYY140 pKa = 10.61 GNAIGIYY147 pKa = 9.34 GYY149 pKa = 10.66 SATGDD154 pKa = 3.51 VAINNAGDD162 pKa = 3.28 ITAYY166 pKa = 10.21 SYY168 pKa = 11.53 SGLADD173 pKa = 4.9 GIFASGDD180 pKa = 3.47 HH181 pKa = 5.61 VVVDD185 pKa = 3.39 NSGAIDD191 pKa = 3.82 AYY193 pKa = 10.5 GYY195 pKa = 10.8 AWAAGIEE202 pKa = 4.05 ARR204 pKa = 11.84 ANTSVEE210 pKa = 4.14 VTNSGDD216 pKa = 3.06 IYY218 pKa = 11.06 AGIYY222 pKa = 8.15 ATGGAYY228 pKa = 10.11 GIYY231 pKa = 10.38 ASGHH235 pKa = 6.46 DD236 pKa = 3.57 ATVVNDD242 pKa = 3.82 GGITARR248 pKa = 11.84 GYY250 pKa = 8.91 YY251 pKa = 8.81 ATGIRR256 pKa = 11.84 TQTYY260 pKa = 8.41 GDD262 pKa = 3.63 AVIDD266 pKa = 3.54 NTGNIDD272 pKa = 3.34 AGTTTGSALAWGINAVASGEE292 pKa = 4.11 GAAIQIDD299 pKa = 3.93 NTGSIQAAAVYY310 pKa = 9.49 GATGIEE316 pKa = 4.82 GIATGTGGTVQVNNSGDD333 pKa = 3.14 IVVQQNRR340 pKa = 11.84 AITSYY345 pKa = 10.89 GGYY348 pKa = 10.38 GILASADD355 pKa = 2.94 GDD357 pKa = 3.79 ASINNSGDD365 pKa = 3.0 IVVASKK371 pKa = 11.19 GPAFGLVGMSFSGEE385 pKa = 3.32 ASVTNEE391 pKa = 3.4 GNVAVSSTVNSTGVTPTGLLAFGANGTAAVEE422 pKa = 4.02 NSGTVVATGVRR433 pKa = 11.84 RR434 pKa = 11.84 AAGIDD439 pKa = 3.03 ASAYY443 pKa = 10.17 GDD445 pKa = 3.56 VSIDD449 pKa = 3.37 NSGDD453 pKa = 3.0 VSAYY457 pKa = 7.78 ATGMFTNTRR466 pKa = 11.84 ADD468 pKa = 3.91 GIKK471 pKa = 10.15 AVSGSGDD478 pKa = 2.98 ITLDD482 pKa = 3.44 NSGSIYY488 pKa = 10.8 ASGTTVGLGVYY499 pKa = 10.06 AVAEE503 pKa = 4.14 NGNIAINNAVDD514 pKa = 4.02 GSIGAYY520 pKa = 9.61 SYY522 pKa = 11.16 YY523 pKa = 10.65 GVARR527 pKa = 11.84 GVFAYY532 pKa = 7.75 ATHH535 pKa = 6.85 GDD537 pKa = 3.42 IAIDD541 pKa = 3.41 SAGAIDD547 pKa = 4.06 AYY549 pKa = 10.63 GWTRR553 pKa = 11.84 TYY555 pKa = 11.38 GVMALAPGGDD565 pKa = 3.54 AAVDD569 pKa = 3.66 SSGTIYY575 pKa = 10.85 ARR577 pKa = 11.84 SGSNNATGVLASTDD591 pKa = 3.21 QGAASITNSGDD602 pKa = 2.95 IQVISRR608 pKa = 11.84 TGSYY612 pKa = 10.17 GVRR615 pKa = 11.84 AYY617 pKa = 10.27 GYY619 pKa = 10.87 AGADD623 pKa = 3.42 VGNSGSIYY631 pKa = 9.99 AASTNGVAVGMMAQAGDD648 pKa = 3.68 GNATVTNTGGSIEE661 pKa = 4.31 VKK663 pKa = 10.33 AATAAFGIFADD674 pKa = 4.15 SAYY677 pKa = 10.86 GDD679 pKa = 3.57 ATVTNASEE687 pKa = 4.06 ISALGVNSAATGIRR701 pKa = 11.84 ATASGDD707 pKa = 3.29 VDD709 pKa = 3.38 ITNSATIDD717 pKa = 3.3 AMSNGDD723 pKa = 3.76 AIGIYY728 pKa = 10.01 GYY730 pKa = 11.0 SLAADD735 pKa = 3.56 VTIDD739 pKa = 3.39 NSGDD743 pKa = 2.99 ITASSSYY750 pKa = 10.83 GLADD754 pKa = 4.96 GIFASGQDD762 pKa = 3.49 VAVTNSGAVSVTSVYY777 pKa = 10.96 GDD779 pKa = 3.2 WAAGIEE785 pKa = 4.29 AQGSNSTQVSNTGDD799 pKa = 3.09 ITASAMPFLQVEE811 pKa = 4.67 DD812 pKa = 4.26 GTSVLYY818 pKa = 9.5 STAGGQAFGIYY829 pKa = 8.51 ATAGVGNGVVTNSGSLVVQGGYY851 pKa = 8.63 ATGIEE856 pKa = 4.14 AQSEE860 pKa = 4.39 GDD862 pKa = 3.53 LSVTNSGDD870 pKa = 2.98 IFAGTGLGAYY880 pKa = 9.32 YY881 pKa = 10.53 NSNNGYY887 pKa = 9.3 GYY889 pKa = 11.1 YY890 pKa = 10.22 YY891 pKa = 9.03 GTLMAIGINASGNGEE906 pKa = 4.03 GMHH909 pKa = 5.83 VAVANTGDD917 pKa = 3.26 IAANGIFGASGISATASGLGGTAVVTNTGDD947 pKa = 2.84 ITAAQYY953 pKa = 10.56 VAGGYY958 pKa = 9.7 GAFGIVASTDD968 pKa = 3.18 GDD970 pKa = 3.87 SGIDD974 pKa = 3.24 NGGAITVTSGGTGYY988 pKa = 10.9 GVAALSFAGDD998 pKa = 3.3 AVVVNSGDD1006 pKa = 3.58 VSVEE1010 pKa = 3.52 ASTAASKK1017 pKa = 11.3 YY1018 pKa = 7.9 YY1019 pKa = 10.8 GSYY1022 pKa = 10.93 GLLAFAGNGYY1032 pKa = 10.19 AAVDD1036 pKa = 3.54 NSGSVSVSNAGIMAAPARR1054 pKa = 11.84 AVDD1057 pKa = 3.54 AQGQQGVGVVNSGALYY1073 pKa = 10.82 ADD1075 pKa = 3.73 GKK1077 pKa = 9.16 YY1078 pKa = 10.48 AYY1080 pKa = 10.15 GVFAVSAGGDD1090 pKa = 3.36 VAVNNTADD1098 pKa = 3.75 GDD1100 pKa = 3.81 IGFYY1104 pKa = 10.64 SYY1106 pKa = 11.18 LGYY1109 pKa = 8.98 GTGILGVSEE1118 pKa = 4.75 GGDD1121 pKa = 3.41 VAIGNAGAIAGYY1133 pKa = 9.92 GYY1135 pKa = 10.17 SQAAGIFGGASTGDD1149 pKa = 3.14 VDD1151 pKa = 4.92 VSNASTGSIDD1161 pKa = 3.56 VVSAGFAVGIFARR1174 pKa = 11.84 ADD1176 pKa = 3.29 YY1177 pKa = 8.41 GTATIVNSGDD1187 pKa = 2.73 ISASVFPDD1195 pKa = 3.01 TGYY1198 pKa = 10.88 QGDD1201 pKa = 3.64 VAYY1204 pKa = 10.43 GLLATSDD1211 pKa = 3.7 YY1212 pKa = 11.14 GYY1214 pKa = 11.16 AAAGNSGDD1222 pKa = 3.67 IQADD1226 pKa = 4.44 GYY1228 pKa = 9.19 YY1229 pKa = 9.28 TATGIAARR1237 pKa = 11.84 SLLGSTVSTAAGSHH1251 pKa = 5.9 INATADD1257 pKa = 3.38 YY1258 pKa = 9.58 VAIGIEE1264 pKa = 4.28 GRR1266 pKa = 11.84 AEE1268 pKa = 4.25 GGDD1271 pKa = 3.54 VTVGSAGDD1279 pKa = 3.25 IATAGGYY1286 pKa = 10.49 GSVGIQAYY1294 pKa = 10.35 SAMGDD1299 pKa = 3.42 VTVGNTGHH1307 pKa = 6.29 ITAASGYY1314 pKa = 9.14 GIAIGATAYY1323 pKa = 9.74 TMEE1326 pKa = 4.38 GLVTVTNNGLIEE1338 pKa = 4.72 ADD1340 pKa = 3.53 AAIASYY1346 pKa = 10.7 GILADD1351 pKa = 4.06 SYY1353 pKa = 11.78 AGDD1356 pKa = 3.51 VVVNLGSNGRR1366 pKa = 11.84 VEE1368 pKa = 5.16 ASAGMAAFGVVVNSGDD1384 pKa = 3.67 GDD1386 pKa = 4.04 STVNNAGKK1394 pKa = 9.35 IHH1396 pKa = 6.99 AGGATYY1402 pKa = 11.03 NFGVVFEE1409 pKa = 4.58 GMYY1412 pKa = 10.48 GANTLNNLATGVISADD1428 pKa = 3.21 GDD1430 pKa = 3.42 EE1431 pKa = 5.23 GYY1433 pKa = 11.51 AFAVAGNDD1441 pKa = 4.2 GVEE1444 pKa = 4.18 TLNNSGRR1451 pKa = 11.84 ILGAISLYY1459 pKa = 11.1 GGDD1462 pKa = 4.56 DD1463 pKa = 3.58 VFSNKK1468 pKa = 10.09 AGGTWDD1474 pKa = 3.33 VGSTLSTDD1482 pKa = 4.17 FGYY1485 pKa = 11.44 GNDD1488 pKa = 3.73 TLANAAGGTITLNGGQIAFGAGDD1511 pKa = 3.69 DD1512 pKa = 4.32 TINNAGLIGLTNGTITMGDD1531 pKa = 3.59 ASPVVPLQAAAPQAVEE1547 pKa = 3.94 MNAFNNSGTLKK1558 pKa = 10.29 IVGSSVVDD1566 pKa = 3.29 TAGGVFTNTGLVDD1579 pKa = 4.05 FVNGVTTDD1587 pKa = 3.43 ALSLDD1592 pKa = 3.65 GTLAGSGQMAIDD1604 pKa = 3.73 VNFNDD1609 pKa = 4.07 LTADD1613 pKa = 3.39 QLAINGDD1620 pKa = 3.7 IASGTAQTVNVKK1632 pKa = 10.34 FIGVPTLGAPAVEE1645 pKa = 4.62 FASVTGTSAAGSFVGGHH1662 pKa = 4.25 VLGYY1666 pKa = 9.47 NQNANFLTLGVDD1678 pKa = 3.49 VTSVLNAGNAADD1690 pKa = 4.75 DD1691 pKa = 4.28 LFSVGLRR1698 pKa = 11.84 VDD1700 pKa = 3.72 GLSDD1704 pKa = 4.14 GGTLAANAASGAAGFMNSQVGTFRR1728 pKa = 11.84 QRR1730 pKa = 11.84 LGVNPYY1736 pKa = 10.14 GDD1738 pKa = 3.57 AGKK1741 pKa = 10.48 VLSAFVRR1748 pKa = 11.84 VYY1750 pKa = 10.62 TDD1752 pKa = 3.0 QGDD1755 pKa = 3.89 AKK1757 pKa = 9.06 PTHH1760 pKa = 6.26 LAGNFGQGGNFAYY1773 pKa = 9.81 EE1774 pKa = 3.73 QASWGRR1780 pKa = 11.84 EE1781 pKa = 3.54 FGVNANLFGNLHH1793 pKa = 6.66 AGVVMGNAASRR1804 pKa = 11.84 QRR1806 pKa = 11.84 LTDD1809 pKa = 3.44 GEE1811 pKa = 4.56 GQNRR1815 pKa = 11.84 MNGTTFGAYY1824 pKa = 7.35 ATWYY1828 pKa = 9.34 VPEE1831 pKa = 4.35 GLYY1834 pKa = 10.64 VDD1836 pKa = 5.31 LSGRR1840 pKa = 11.84 WMGADD1845 pKa = 2.93 IRR1847 pKa = 11.84 SDD1849 pKa = 3.53 SVGANLQSRR1858 pKa = 11.84 ARR1860 pKa = 11.84 TSAVSLEE1867 pKa = 4.69 AGYY1870 pKa = 9.59 EE1871 pKa = 3.97 WKK1873 pKa = 10.6 LGSVNVVPQAQYY1885 pKa = 10.49 IRR1887 pKa = 11.84 TRR1889 pKa = 11.84 VEE1891 pKa = 3.89 DD1892 pKa = 3.08 VRR1894 pKa = 11.84 TFYY1897 pKa = 11.39 GEE1899 pKa = 4.19 SLDD1902 pKa = 3.71 FTVQGGTFEE1911 pKa = 4.2 RR1912 pKa = 11.84 GRR1914 pKa = 11.84 VGVEE1918 pKa = 3.66 VNKK1921 pKa = 9.4 TFQSGDD1927 pKa = 3.54 IRR1929 pKa = 11.84 WTPYY1933 pKa = 10.85 GSISALRR1940 pKa = 11.84 DD1941 pKa = 3.41 SGGKK1945 pKa = 7.85 TSYY1948 pKa = 11.24 AVGDD1952 pKa = 4.23 FYY1954 pKa = 11.73 GSTGVSGSRR1963 pKa = 11.84 AAAEE1967 pKa = 4.32 LGVGVQKK1974 pKa = 11.1 GGLGVTLGVNWLDD1987 pKa = 3.74 GGSLKK1992 pKa = 10.99 SFIGGQANLRR2002 pKa = 11.84 YY2003 pKa = 9.59 SWW2005 pKa = 3.2
Molecular weight: 197.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 3.859
IPC_protein 3.923
Toseland 3.681
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.745
Grimsley 3.579
Solomon 3.923
Lehninger 3.884
Nozaki 4.024
DTASelect 4.329
Thurlkill 3.732
EMBOSS 3.884
Sillero 4.05
Patrickios 0.451
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|A0A5R9PHK0|A0A5R9PHK0_9GAMM NrdJb OS=Thermomonas fusca OX=215690 GN=E5S66_02270 PE=4 SV=1
MM1 pKa = 6.72 THH3 pKa = 7.26 RR4 pKa = 11.84 DD5 pKa = 3.18 LDD7 pKa = 3.73 ALLRR11 pKa = 11.84 QLVILGALAVLLIPAARR28 pKa = 11.84 GSSALLGWLPLWLLGMPLAAWWSLRR53 pKa = 11.84 RR54 pKa = 11.84 FPLPTLALRR63 pKa = 11.84 LPQRR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 VQARR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 GHH78 pKa = 5.53 RR79 pKa = 11.84 VARR82 pKa = 11.84 ALRR85 pKa = 11.84 IARR88 pKa = 11.84 RR89 pKa = 11.84 AA90 pKa = 3.26
Molecular weight: 10.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.93
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2622
0
2622
884264
25
2306
337.2
36.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.912 ± 0.072
0.84 ± 0.013
5.866 ± 0.033
5.294 ± 0.038
3.325 ± 0.029
8.692 ± 0.05
2.2 ± 0.024
4.086 ± 0.035
2.855 ± 0.043
11.153 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.249 ± 0.023
2.385 ± 0.029
5.257 ± 0.033
3.753 ± 0.031
7.803 ± 0.052
4.89 ± 0.038
4.523 ± 0.043
7.256 ± 0.039
1.492 ± 0.021
2.166 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here