Escherichia phage Shashou

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8RL76|A0A5B8RL76_9CAUD Phosphatase OS=Escherichia phage Shashou OX=2580403 GN=CPT_Shashou_023 PE=4 SV=1
MM1 pKa = 8.35RR2 pKa = 11.84IRR4 pKa = 11.84ITDD7 pKa = 3.54VEE9 pKa = 4.55QTCPEE14 pKa = 4.25EE15 pKa = 4.13YY16 pKa = 10.26QDD18 pKa = 4.28VSLAYY23 pKa = 10.19FGVSVGDD30 pKa = 3.8EE31 pKa = 4.03FDD33 pKa = 3.71VGISCEE39 pKa = 3.89YY40 pKa = 10.54GFVVNHH46 pKa = 6.55RR47 pKa = 11.84GEE49 pKa = 4.13NLFVRR54 pKa = 11.84NSEE57 pKa = 4.32CEE59 pKa = 4.13VLEE62 pKa = 4.59DD63 pKa = 4.01

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8RL60|A0A5B8RL60_9CAUD Portal protein OS=Escherichia phage Shashou OX=2580403 GN=CPT_Shashou_003 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.35ILKK9 pKa = 10.63RR10 pKa = 11.84SGLAPLSPRR19 pKa = 11.84ANTQIYY25 pKa = 9.15KK26 pKa = 10.26RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84NTLYY33 pKa = 10.28PEE35 pKa = 3.64IQEE38 pKa = 3.76RR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 10.35AIRR44 pKa = 11.84ACGFQNGKK52 pKa = 9.72AVNLGEE58 pKa = 4.7FKK60 pKa = 9.58TQEE63 pKa = 3.57RR64 pKa = 11.84AAIANRR70 pKa = 11.84LFNYY74 pKa = 7.62WKK76 pKa = 10.65SLGYY80 pKa = 10.66DD81 pKa = 4.56DD82 pKa = 6.25IPTKK86 pKa = 8.88PQRR89 pKa = 11.84RR90 pKa = 11.84QYY92 pKa = 9.93IWRR95 pKa = 11.84HH96 pKa = 4.21KK97 pKa = 10.55

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

13917

29

851

180.7

20.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.47 ± 0.526

1.099 ± 0.117

6.05 ± 0.212

6.647 ± 0.431

3.866 ± 0.13

7.401 ± 0.265

1.645 ± 0.181

5.245 ± 0.129

5.978 ± 0.326

7.473 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.147

4.656 ± 0.262

3.88 ± 0.225

3.679 ± 0.25

5.576 ± 0.239

5.763 ± 0.267

6.496 ± 0.396

7.581 ± 0.298

1.387 ± 0.116

3.629 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski