Phytohabitans flavus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9064 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6F8Y4D7|A0A6F8Y4D7_9ACTN Uncharacterized protein OS=Phytohabitans flavus OX=1076124 GN=Pflav_073030 PE=4 SV=1
MM1 pKa = 7.86 SEE3 pKa = 4.59 FDD5 pKa = 5.56 DD6 pKa = 4.04 PTLTEE11 pKa = 4.7 DD12 pKa = 3.99 PGTGHH17 pKa = 6.87 EE18 pKa = 4.58 GFGNAGDD25 pKa = 4.5 SGHH28 pKa = 6.94 DD29 pKa = 3.35 QPGYY33 pKa = 10.04 DD34 pKa = 4.83 QPGHH38 pKa = 6.13 DD39 pKa = 4.14 QPPYY43 pKa = 10.85 GHH45 pKa = 7.13 DD46 pKa = 3.18 QPHH49 pKa = 6.28 YY50 pKa = 9.4 EE51 pKa = 4.26 EE52 pKa = 4.32 PHH54 pKa = 5.77 YY55 pKa = 10.18 GQPDD59 pKa = 4.03 SIVQHH64 pKa = 6.27 NPDD67 pKa = 3.57 GSTDD71 pKa = 3.55 TVVDD75 pKa = 4.59 LDD77 pKa = 3.78 GDD79 pKa = 4.24 GYY81 pKa = 11.67 ADD83 pKa = 3.76 VVKK86 pKa = 10.62 HH87 pKa = 5.92 DD88 pKa = 4.27 TDD90 pKa = 3.75 SDD92 pKa = 4.37 GYY94 pKa = 11.73 ADD96 pKa = 3.78 TTYY99 pKa = 11.25 VDD101 pKa = 3.84 ANHH104 pKa = 7.41 DD105 pKa = 3.71 GRR107 pKa = 11.84 LDD109 pKa = 4.11 TILKK113 pKa = 10.57 DD114 pKa = 3.71 YY115 pKa = 11.36 DD116 pKa = 4.09 DD117 pKa = 6.47 DD118 pKa = 6.58 GDD120 pKa = 4.29 LDD122 pKa = 4.31 AAFADD127 pKa = 4.28 TNADD131 pKa = 3.06 GRR133 pKa = 11.84 VDD135 pKa = 3.53 YY136 pKa = 10.95 AAADD140 pKa = 3.96 LNDD143 pKa = 4.31 DD144 pKa = 3.87 NKK146 pKa = 10.36 VDD148 pKa = 4.43 HH149 pKa = 6.38 VTLDD153 pKa = 3.05 TDD155 pKa = 4.03 YY156 pKa = 11.57 DD157 pKa = 4.02 GKK159 pKa = 11.07 ADD161 pKa = 3.43 TWIADD166 pKa = 3.24 VDD168 pKa = 4.09 YY169 pKa = 11.27 DD170 pKa = 4.04 GQPDD174 pKa = 3.77 VAFVDD179 pKa = 4.31 TNHH182 pKa = 7.44 DD183 pKa = 3.64 GTPDD187 pKa = 3.5 VAVSDD192 pKa = 4.51 ANHH195 pKa = 7.31 DD196 pKa = 3.88 GTPEE200 pKa = 3.89 QQTAAPQTVTPPPPVNPYY218 pKa = 9.87 AASS221 pKa = 3.26
Molecular weight: 23.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.554
IPC_protein 3.617
Toseland 3.363
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.63
Rodwell 3.439
Grimsley 3.274
Solomon 3.643
Lehninger 3.592
Nozaki 3.757
DTASelect 4.101
Thurlkill 3.439
EMBOSS 3.63
Sillero 3.745
Patrickios 0.579
IPC_peptide 3.63
IPC2_peptide 3.719
IPC2.peptide.svr19 3.71
Protein with the highest isoelectric point:
>tr|A0A6F8Y4C5|A0A6F8Y4C5_9ACTN Uncharacterized protein OS=Phytohabitans flavus OX=1076124 GN=Pflav_073760 PE=4 SV=1
MM1 pKa = 7.69 PAAQPRR7 pKa = 11.84 LPVAHH12 pKa = 7.25 LAGPSLLAARR22 pKa = 11.84 MPVVRR27 pKa = 11.84 VRR29 pKa = 11.84 LLVAHH34 pKa = 7.09 LAGLSLPVPRR44 pKa = 11.84 TPVARR49 pKa = 11.84 VRR51 pKa = 11.84 LSVVHH56 pKa = 6.54 LVGLSLLAARR66 pKa = 11.84 MPAARR71 pKa = 11.84 VRR73 pKa = 11.84 LLAVPLSGAWLARR86 pKa = 11.84 VVAPAVV92 pKa = 3.25
Molecular weight: 9.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.237
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9064
0
9064
2839989
39
6022
313.3
33.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.947 ± 0.041
0.773 ± 0.008
5.951 ± 0.021
5.155 ± 0.022
2.756 ± 0.016
9.281 ± 0.025
2.09 ± 0.012
3.388 ± 0.018
1.738 ± 0.016
10.19 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.727 ± 0.01
1.872 ± 0.018
6.268 ± 0.021
2.779 ± 0.014
8.257 ± 0.032
5.113 ± 0.023
6.096 ± 0.025
8.84 ± 0.024
1.64 ± 0.012
2.139 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here