Amino acid dipepetide frequency for Clostridium phage phiCP13O

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.015AlaAla: 2.015 ± 0.912
0.356AlaCys: 0.356 ± 0.22
2.371AlaAsp: 2.371 ± 0.607
3.438AlaGlu: 3.438 ± 0.634
2.489AlaPhe: 2.489 ± 0.725
2.015AlaGly: 2.015 ± 0.772
0.83AlaHis: 0.83 ± 0.312
6.401AlaIle: 6.401 ± 0.842
4.386AlaLys: 4.386 ± 0.724
5.453AlaLeu: 5.453 ± 1.03
1.541AlaMet: 1.541 ± 0.383
4.03AlaAsn: 4.03 ± 0.939
1.067AlaPro: 1.067 ± 0.339
2.015AlaGln: 2.015 ± 0.477
3.082AlaArg: 3.082 ± 0.532
3.082AlaSer: 3.082 ± 0.775
2.845AlaThr: 2.845 ± 0.665
3.082AlaVal: 3.082 ± 0.687
0.474AlaTrp: 0.474 ± 0.268
1.897AlaTyr: 1.897 ± 0.44
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.152
0.356CysCys: 0.356 ± 0.207
0.593CysAsp: 0.593 ± 0.26
1.541CysGlu: 1.541 ± 0.424
0.474CysPhe: 0.474 ± 0.225
0.948CysGly: 0.948 ± 0.32
0.119CysHis: 0.119 ± 0.126
1.067CysIle: 1.067 ± 0.343
1.185CysLys: 1.185 ± 0.345
1.067CysLeu: 1.067 ± 0.326
0.356CysMet: 0.356 ± 0.185
0.474CysAsn: 0.474 ± 0.234
0.356CysPro: 0.356 ± 0.211
0.119CysGln: 0.119 ± 0.103
0.237CysArg: 0.237 ± 0.164
1.067CysSer: 1.067 ± 0.323
0.474CysThr: 0.474 ± 0.242
0.356CysVal: 0.356 ± 0.231
0.0CysTrp: 0.0 ± 0.0
0.711CysTyr: 0.711 ± 0.342
0.0CysXaa: 0.0 ± 0.0
Asp
3.675AspAla: 3.675 ± 0.7
0.474AspCys: 0.474 ± 0.276
2.015AspAsp: 2.015 ± 0.518
4.86AspGlu: 4.86 ± 0.76
2.963AspPhe: 2.963 ± 0.617
3.438AspGly: 3.438 ± 0.674
0.356AspHis: 0.356 ± 0.212
5.453AspIle: 5.453 ± 0.783
5.69AspLys: 5.69 ± 0.852
6.164AspLeu: 6.164 ± 0.709
2.015AspMet: 2.015 ± 0.476
2.726AspAsn: 2.726 ± 0.59
0.474AspPro: 0.474 ± 0.225
0.83AspGln: 0.83 ± 0.441
1.422AspArg: 1.422 ± 0.489
2.963AspSer: 2.963 ± 0.55
3.556AspThr: 3.556 ± 0.659
3.201AspVal: 3.201 ± 0.64
1.422AspTrp: 1.422 ± 0.429
3.556AspTyr: 3.556 ± 0.639
0.0AspXaa: 0.0 ± 0.0
Glu
3.556GluAla: 3.556 ± 0.627
0.948GluCys: 0.948 ± 0.329
5.097GluAsp: 5.097 ± 1.126
9.128GluGlu: 9.128 ± 1.288
3.793GluPhe: 3.793 ± 0.679
6.164GluGly: 6.164 ± 0.802
1.541GluHis: 1.541 ± 0.418
8.89GluIle: 8.89 ± 1.182
8.772GluLys: 8.772 ± 1.12
6.994GluLeu: 6.994 ± 0.929
4.267GluMet: 4.267 ± 0.806
5.453GluAsn: 5.453 ± 0.894
1.66GluPro: 1.66 ± 0.415
3.438GluGln: 3.438 ± 0.675
2.489GluArg: 2.489 ± 0.656
3.082GluSer: 3.082 ± 0.61
4.386GluThr: 4.386 ± 0.656
4.86GluVal: 4.86 ± 0.854
0.83GluTrp: 0.83 ± 0.311
3.201GluTyr: 3.201 ± 0.532
0.0GluXaa: 0.0 ± 0.0
Phe
1.541PheAla: 1.541 ± 0.368
0.474PheCys: 0.474 ± 0.225
2.015PheAsp: 2.015 ± 0.453
4.267PheGlu: 4.267 ± 0.666
0.593PhePhe: 0.593 ± 0.298
2.015PheGly: 2.015 ± 0.435
0.119PheHis: 0.119 ± 0.103
4.03PheIle: 4.03 ± 0.707
4.267PheLys: 4.267 ± 0.61
2.726PheLeu: 2.726 ± 0.465
1.778PheMet: 1.778 ± 0.444
3.319PheAsn: 3.319 ± 0.568
0.83PhePro: 0.83 ± 0.3
1.067PheGln: 1.067 ± 0.286
1.541PheArg: 1.541 ± 0.361
3.201PheSer: 3.201 ± 0.603
2.963PheThr: 2.963 ± 0.521
2.726PheVal: 2.726 ± 0.594
0.593PheTrp: 0.593 ± 0.294
1.778PheTyr: 1.778 ± 0.486
0.119PheXaa: 0.119 ± 0.125
Gly
3.912GlyAla: 3.912 ± 0.854
0.948GlyCys: 0.948 ± 0.273
2.489GlyAsp: 2.489 ± 0.523
4.267GlyGlu: 4.267 ± 0.735
2.963GlyPhe: 2.963 ± 0.665
3.556GlyGly: 3.556 ± 0.668
0.711GlyHis: 0.711 ± 0.303
3.912GlyIle: 3.912 ± 1.053
4.86GlyLys: 4.86 ± 0.787
5.571GlyLeu: 5.571 ± 0.774
1.185GlyMet: 1.185 ± 0.346
3.438GlyAsn: 3.438 ± 0.981
0.237GlyPro: 0.237 ± 0.158
1.66GlyGln: 1.66 ± 0.55
2.252GlyArg: 2.252 ± 0.486
3.556GlySer: 3.556 ± 0.546
4.03GlyThr: 4.03 ± 0.646
4.267GlyVal: 4.267 ± 0.528
0.474GlyTrp: 0.474 ± 0.231
2.726GlyTyr: 2.726 ± 0.547
0.0GlyXaa: 0.0 ± 0.0
His
0.237HisAla: 0.237 ± 0.163
0.119HisCys: 0.119 ± 0.114
1.067HisAsp: 1.067 ± 0.399
0.83HisGlu: 0.83 ± 0.333
0.948HisPhe: 0.948 ± 0.294
0.711HisGly: 0.711 ± 0.282
0.119HisHis: 0.119 ± 0.109
0.83HisIle: 0.83 ± 0.248
1.067HisLys: 1.067 ± 0.331
1.304HisLeu: 1.304 ± 0.294
0.119HisMet: 0.119 ± 0.126
0.237HisAsn: 0.237 ± 0.157
0.593HisPro: 0.593 ± 0.245
0.356HisGln: 0.356 ± 0.186
0.593HisArg: 0.593 ± 0.278
0.83HisSer: 0.83 ± 0.312
0.83HisThr: 0.83 ± 0.32
0.356HisVal: 0.356 ± 0.213
0.119HisTrp: 0.119 ± 0.123
1.067HisTyr: 1.067 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
5.69IleAla: 5.69 ± 0.777
1.304IleCys: 1.304 ± 0.453
6.283IleAsp: 6.283 ± 0.754
8.179IleGlu: 8.179 ± 1.07
2.371IlePhe: 2.371 ± 0.531
3.319IleGly: 3.319 ± 0.607
0.711IleHis: 0.711 ± 0.279
5.571IleIle: 5.571 ± 0.843
9.365IleLys: 9.365 ± 0.981
6.046IleLeu: 6.046 ± 0.75
2.608IleMet: 2.608 ± 0.583
5.334IleAsn: 5.334 ± 0.755
3.201IlePro: 3.201 ± 0.483
2.489IleGln: 2.489 ± 0.622
2.608IleArg: 2.608 ± 0.562
6.401IleSer: 6.401 ± 0.867
3.793IleThr: 3.793 ± 0.719
3.675IleVal: 3.675 ± 0.561
0.948IleTrp: 0.948 ± 0.3
3.201IleTyr: 3.201 ± 0.58
0.0IleXaa: 0.0 ± 0.0
Lys
5.69LysAla: 5.69 ± 0.877
0.711LysCys: 0.711 ± 0.314
4.267LysAsp: 4.267 ± 0.746
11.143LysGlu: 11.143 ± 1.47
4.03LysPhe: 4.03 ± 0.619
5.927LysGly: 5.927 ± 0.804
1.185LysHis: 1.185 ± 0.383
7.705LysIle: 7.705 ± 0.771
7.112LysLys: 7.112 ± 0.855
7.705LysLeu: 7.705 ± 0.816
2.252LysMet: 2.252 ± 0.504
5.334LysAsn: 5.334 ± 0.772
3.082LysPro: 3.082 ± 0.613
4.149LysGln: 4.149 ± 0.727
4.979LysArg: 4.979 ± 0.957
3.912LysSer: 3.912 ± 0.733
4.742LysThr: 4.742 ± 0.63
7.468LysVal: 7.468 ± 0.765
0.83LysTrp: 0.83 ± 0.266
3.319LysTyr: 3.319 ± 0.555
0.0LysXaa: 0.0 ± 0.0
Leu
5.927LeuAla: 5.927 ± 0.862
0.593LeuCys: 0.593 ± 0.305
4.979LeuAsp: 4.979 ± 0.636
8.535LeuGlu: 8.535 ± 0.845
2.371LeuPhe: 2.371 ± 0.481
4.505LeuGly: 4.505 ± 0.753
1.541LeuHis: 1.541 ± 0.366
5.69LeuIle: 5.69 ± 0.932
8.061LeuLys: 8.061 ± 0.894
5.571LeuLeu: 5.571 ± 0.749
3.319LeuMet: 3.319 ± 0.49
5.571LeuAsn: 5.571 ± 0.849
1.541LeuPro: 1.541 ± 0.373
3.438LeuGln: 3.438 ± 0.617
3.319LeuArg: 3.319 ± 0.588
4.86LeuSer: 4.86 ± 0.836
4.979LeuThr: 4.979 ± 1.034
4.267LeuVal: 4.267 ± 0.772
0.83LeuTrp: 0.83 ± 0.317
4.267LeuTyr: 4.267 ± 0.892
0.0LeuXaa: 0.0 ± 0.0
Met
2.371MetAla: 2.371 ± 0.588
0.474MetCys: 0.474 ± 0.217
1.897MetAsp: 1.897 ± 0.547
2.845MetGlu: 2.845 ± 0.709
1.067MetPhe: 1.067 ± 0.359
2.252MetGly: 2.252 ± 0.447
0.0MetHis: 0.0 ± 0.0
2.726MetIle: 2.726 ± 0.538
2.963MetLys: 2.963 ± 0.53
2.134MetLeu: 2.134 ± 0.515
0.83MetMet: 0.83 ± 0.293
2.252MetAsn: 2.252 ± 0.533
0.948MetPro: 0.948 ± 0.308
1.185MetGln: 1.185 ± 0.329
1.422MetArg: 1.422 ± 0.35
1.66MetSer: 1.66 ± 0.447
1.897MetThr: 1.897 ± 0.427
1.185MetVal: 1.185 ± 0.392
0.356MetTrp: 0.356 ± 0.173
0.593MetTyr: 0.593 ± 0.275
0.0MetXaa: 0.0 ± 0.0
Asn
3.675AsnAla: 3.675 ± 0.789
0.593AsnCys: 0.593 ± 0.27
3.675AsnAsp: 3.675 ± 0.732
3.319AsnGlu: 3.319 ± 0.501
3.082AsnPhe: 3.082 ± 0.559
3.793AsnGly: 3.793 ± 0.594
1.304AsnHis: 1.304 ± 0.424
5.216AsnIle: 5.216 ± 0.629
6.283AsnLys: 6.283 ± 0.769
3.438AsnLeu: 3.438 ± 0.71
1.541AsnMet: 1.541 ± 0.381
4.386AsnAsn: 4.386 ± 0.774
1.897AsnPro: 1.897 ± 0.47
1.897AsnGln: 1.897 ± 0.428
3.438AsnArg: 3.438 ± 0.537
3.675AsnSer: 3.675 ± 0.72
4.03AsnThr: 4.03 ± 0.769
3.201AsnVal: 3.201 ± 0.46
1.185AsnTrp: 1.185 ± 0.409
3.201AsnTyr: 3.201 ± 0.562
0.0AsnXaa: 0.0 ± 0.0
Pro
0.593ProAla: 0.593 ± 0.278
0.356ProCys: 0.356 ± 0.184
1.422ProAsp: 1.422 ± 0.461
1.304ProGlu: 1.304 ± 0.371
1.778ProPhe: 1.778 ± 0.522
0.474ProGly: 0.474 ± 0.227
0.474ProHis: 0.474 ± 0.2
2.608ProIle: 2.608 ± 0.549
2.252ProLys: 2.252 ± 0.475
3.201ProLeu: 3.201 ± 0.644
0.83ProMet: 0.83 ± 0.338
2.252ProAsn: 2.252 ± 0.594
0.356ProPro: 0.356 ± 0.174
1.422ProGln: 1.422 ± 0.428
0.593ProArg: 0.593 ± 0.246
0.948ProSer: 0.948 ± 0.334
1.66ProThr: 1.66 ± 0.425
1.185ProVal: 1.185 ± 0.405
0.0ProTrp: 0.0 ± 0.0
1.541ProTyr: 1.541 ± 0.402
0.0ProXaa: 0.0 ± 0.0
Gln
2.134GlnAla: 2.134 ± 0.635
0.119GlnCys: 0.119 ± 0.128
2.015GlnAsp: 2.015 ± 0.445
2.726GlnGlu: 2.726 ± 0.601
1.66GlnPhe: 1.66 ± 0.499
2.726GlnGly: 2.726 ± 0.628
0.356GlnHis: 0.356 ± 0.206
2.252GlnIle: 2.252 ± 0.564
2.845GlnLys: 2.845 ± 0.728
3.201GlnLeu: 3.201 ± 0.774
1.067GlnMet: 1.067 ± 0.425
1.897GlnAsn: 1.897 ± 0.478
0.948GlnPro: 0.948 ± 0.303
1.304GlnGln: 1.304 ± 0.462
1.778GlnArg: 1.778 ± 0.502
1.304GlnSer: 1.304 ± 0.417
1.422GlnThr: 1.422 ± 0.331
1.422GlnVal: 1.422 ± 0.378
0.474GlnTrp: 0.474 ± 0.206
1.66GlnTyr: 1.66 ± 0.514
0.0GlnXaa: 0.0 ± 0.0
Arg
1.185ArgAla: 1.185 ± 0.384
0.83ArgCys: 0.83 ± 0.285
2.489ArgAsp: 2.489 ± 0.571
4.623ArgGlu: 4.623 ± 0.863
2.134ArgPhe: 2.134 ± 0.525
2.489ArgGly: 2.489 ± 0.526
0.119ArgHis: 0.119 ± 0.128
3.082ArgIle: 3.082 ± 0.771
3.201ArgLys: 3.201 ± 0.689
3.438ArgLeu: 3.438 ± 0.744
1.422ArgMet: 1.422 ± 0.383
2.845ArgAsn: 2.845 ± 0.568
1.185ArgPro: 1.185 ± 0.383
1.185ArgGln: 1.185 ± 0.402
1.541ArgArg: 1.541 ± 0.372
1.541ArgSer: 1.541 ± 0.442
2.015ArgThr: 2.015 ± 0.449
2.134ArgVal: 2.134 ± 0.5
0.711ArgTrp: 0.711 ± 0.336
2.371ArgTyr: 2.371 ± 0.581
0.0ArgXaa: 0.0 ± 0.0
Ser
2.845SerAla: 2.845 ± 0.68
0.83SerCys: 0.83 ± 0.267
3.912SerAsp: 3.912 ± 0.884
3.319SerGlu: 3.319 ± 0.687
1.897SerPhe: 1.897 ± 0.431
3.675SerGly: 3.675 ± 0.878
0.711SerHis: 0.711 ± 0.288
5.334SerIle: 5.334 ± 0.754
6.283SerLys: 6.283 ± 0.845
4.979SerLeu: 4.979 ± 0.707
1.422SerMet: 1.422 ± 0.452
3.201SerAsn: 3.201 ± 0.453
1.067SerPro: 1.067 ± 0.334
2.134SerGln: 2.134 ± 0.428
2.608SerArg: 2.608 ± 0.525
2.015SerSer: 2.015 ± 0.45
2.252SerThr: 2.252 ± 0.413
2.608SerVal: 2.608 ± 0.548
1.067SerTrp: 1.067 ± 0.361
2.845SerTyr: 2.845 ± 0.53
0.0SerXaa: 0.0 ± 0.0
Thr
2.015ThrAla: 2.015 ± 0.448
0.474ThrCys: 0.474 ± 0.24
3.201ThrAsp: 3.201 ± 0.62
4.03ThrGlu: 4.03 ± 0.846
2.608ThrPhe: 2.608 ± 0.497
3.556ThrGly: 3.556 ± 0.676
1.185ThrHis: 1.185 ± 0.289
4.149ThrIle: 4.149 ± 0.748
4.505ThrLys: 4.505 ± 0.602
5.69ThrLeu: 5.69 ± 0.938
2.489ThrMet: 2.489 ± 0.478
3.438ThrAsn: 3.438 ± 0.59
2.015ThrPro: 2.015 ± 0.526
1.422ThrGln: 1.422 ± 0.343
1.541ThrArg: 1.541 ± 0.537
3.556ThrSer: 3.556 ± 0.671
3.793ThrThr: 3.793 ± 0.698
3.438ThrVal: 3.438 ± 0.59
0.711ThrTrp: 0.711 ± 0.275
1.422ThrTyr: 1.422 ± 0.456
0.0ThrXaa: 0.0 ± 0.0
Val
3.556ValAla: 3.556 ± 0.902
0.83ValCys: 0.83 ± 0.278
3.793ValAsp: 3.793 ± 0.573
4.03ValGlu: 4.03 ± 0.616
1.541ValPhe: 1.541 ± 0.415
3.556ValGly: 3.556 ± 0.699
0.356ValHis: 0.356 ± 0.188
4.03ValIle: 4.03 ± 0.816
7.468ValLys: 7.468 ± 0.963
3.675ValLeu: 3.675 ± 0.565
1.304ValMet: 1.304 ± 0.344
2.371ValAsn: 2.371 ± 0.582
1.778ValPro: 1.778 ± 0.463
1.541ValGln: 1.541 ± 0.378
2.371ValArg: 2.371 ± 0.577
3.675ValSer: 3.675 ± 0.483
2.608ValThr: 2.608 ± 0.549
3.201ValVal: 3.201 ± 0.638
0.711ValTrp: 0.711 ± 0.305
2.371ValTyr: 2.371 ± 0.506
0.0ValXaa: 0.0 ± 0.0
Trp
0.711TrpAla: 0.711 ± 0.292
0.0TrpCys: 0.0 ± 0.0
0.948TrpAsp: 0.948 ± 0.346
1.422TrpGlu: 1.422 ± 0.465
0.593TrpPhe: 0.593 ± 0.328
0.593TrpGly: 0.593 ± 0.244
0.474TrpHis: 0.474 ± 0.314
1.067TrpIle: 1.067 ± 0.417
0.237TrpLys: 0.237 ± 0.144
1.66TrpLeu: 1.66 ± 0.451
0.0TrpMet: 0.0 ± 0.0
0.711TrpAsn: 0.711 ± 0.313
0.119TrpPro: 0.119 ± 0.103
0.474TrpGln: 0.474 ± 0.206
0.711TrpArg: 0.711 ± 0.324
0.711TrpSer: 0.711 ± 0.291
0.593TrpThr: 0.593 ± 0.274
0.593TrpVal: 0.593 ± 0.244
0.119TrpTrp: 0.119 ± 0.116
0.593TrpTyr: 0.593 ± 0.263
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.304TyrAla: 1.304 ± 0.326
0.948TyrCys: 0.948 ± 0.339
2.845TyrAsp: 2.845 ± 0.542
4.623TyrGlu: 4.623 ± 0.865
2.608TyrPhe: 2.608 ± 0.632
1.304TyrGly: 1.304 ± 0.409
0.119TyrHis: 0.119 ± 0.137
3.082TyrIle: 3.082 ± 0.514
4.742TyrLys: 4.742 ± 0.789
4.149TyrLeu: 4.149 ± 0.686
0.593TyrMet: 0.593 ± 0.252
3.556TyrAsn: 3.556 ± 0.769
1.897TyrPro: 1.897 ± 0.475
1.185TyrGln: 1.185 ± 0.364
2.015TyrArg: 2.015 ± 0.484
2.963TyrSer: 2.963 ± 0.605
2.371TyrThr: 2.371 ± 0.574
1.541TyrVal: 1.541 ± 0.444
0.474TyrTrp: 0.474 ± 0.214
2.726TyrTyr: 2.726 ± 0.673
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.119XaaGlu: 0.119 ± 0.125
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (8437 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski