Circovirus-like genome DCCV-2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHA1|A0A190WHA1_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-2 OX=1788442 PE=4 SV=1
MM1 pKa = 7.31FRR3 pKa = 11.84INAKK7 pKa = 9.98NFSLTYY13 pKa = 10.92SNVEE17 pKa = 4.04QQSGVAWLPWDD28 pKa = 3.64KK29 pKa = 11.2EE30 pKa = 4.07SLLRR34 pKa = 11.84HH35 pKa = 6.15LEE37 pKa = 3.98SLGDD41 pKa = 3.77GVIGMVSRR49 pKa = 11.84EE50 pKa = 3.85QHH52 pKa = 5.54QDD54 pKa = 2.74GSTHH58 pKa = 4.74FHH60 pKa = 6.47AWVQFPRR67 pKa = 11.84KK68 pKa = 9.25RR69 pKa = 11.84DD70 pKa = 3.08IRR72 pKa = 11.84ASNFFDD78 pKa = 3.37WQGCHH83 pKa = 6.65PNVQATNNLRR93 pKa = 11.84AWKK96 pKa = 8.97TYY98 pKa = 8.23ICKK101 pKa = 10.71DD102 pKa = 3.17GDD104 pKa = 4.02HH105 pKa = 6.51TPEE108 pKa = 4.73PATPTATGDD117 pKa = 3.94LFVLCHH123 pKa = 6.76SMEE126 pKa = 3.72KK127 pKa = 10.49HH128 pKa = 5.21EE129 pKa = 4.24WVNYY133 pKa = 10.26CIGKK137 pKa = 10.12KK138 pKa = 9.69IGFQYY143 pKa = 10.01MEE145 pKa = 5.02YY146 pKa = 9.72IWKK149 pKa = 9.29DD150 pKa = 3.17CHH152 pKa = 6.4MDD154 pKa = 3.43RR155 pKa = 11.84DD156 pKa = 4.15STIEE160 pKa = 4.15DD161 pKa = 3.79NPPPEE166 pKa = 5.11AVMCAALEE174 pKa = 4.0QFIYY178 pKa = 10.23PSLDD182 pKa = 3.04RR183 pKa = 11.84SPLVLVGDD191 pKa = 4.32TGCGKK196 pKa = 6.67TTWAIKK202 pKa = 9.8NAPKK206 pKa = 9.98PICFVSHH213 pKa = 6.58MDD215 pKa = 3.52QLKK218 pKa = 10.68HH219 pKa = 6.9FDD221 pKa = 3.74PQYY224 pKa = 10.34HH225 pKa = 6.25KK226 pKa = 11.08SIVFDD231 pKa = 4.6DD232 pKa = 4.02MDD234 pKa = 4.55FKK236 pKa = 11.14HH237 pKa = 6.65LPRR240 pKa = 11.84EE241 pKa = 4.28SQIHH245 pKa = 5.91ILDD248 pKa = 3.5MTTPRR253 pKa = 11.84AIHH256 pKa = 6.03RR257 pKa = 11.84RR258 pKa = 11.84YY259 pKa = 10.17GVTVIPAGTKK269 pKa = 10.18KK270 pKa = 10.36IFTANSNPFIEE281 pKa = 5.23DD282 pKa = 3.05PAIIRR287 pKa = 11.84RR288 pKa = 11.84RR289 pKa = 11.84TLRR292 pKa = 11.84IVQGLL297 pKa = 3.52

Molecular weight: 34.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHI4|A0A190WHI4_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-2 OX=1788442 PE=4 SV=1
MM1 pKa = 7.46LKK3 pKa = 10.06ISALPIVMLNNNPGSLGYY21 pKa = 9.68PGIKK25 pKa = 9.24NHH27 pKa = 6.59CSVTLSPLEE36 pKa = 4.14TEE38 pKa = 4.22SLVWCLANNTRR49 pKa = 11.84MEE51 pKa = 4.35VPISTPGFNFLEE63 pKa = 4.36RR64 pKa = 11.84GIYY67 pKa = 9.13EE68 pKa = 4.63PPTSSTGRR76 pKa = 11.84GAIQTSRR83 pKa = 11.84LLITCGRR90 pKa = 11.84GKK92 pKa = 9.87PISAKK97 pKa = 9.07TATIRR102 pKa = 11.84RR103 pKa = 11.84NRR105 pKa = 11.84LRR107 pKa = 11.84RR108 pKa = 11.84LRR110 pKa = 11.84RR111 pKa = 11.84VTYY114 pKa = 9.33LCCATAWKK122 pKa = 8.19NTSGSTIAA130 pKa = 4.87

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

678

130

297

226.0

25.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.49 ± 0.514

2.212 ± 0.616

4.72 ± 1.402

3.097 ± 0.889

5.015 ± 0.891

5.752 ± 0.448

2.802 ± 1.23

5.605 ± 1.241

4.867 ± 0.468

7.08 ± 0.872

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.802 ± 0.14

5.9 ± 1.045

5.9 ± 0.487

4.277 ± 0.883

5.605 ± 0.975

6.785 ± 0.8

10.029 ± 2.35

6.047 ± 0.836

1.622 ± 0.646

3.392 ± 0.61
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski