Bacteroides luti
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3225 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M4WWD3|A0A1M4WWD3_9BACE Bile acid:Na+ symporter BASS family OS=Bacteroides luti OX=1297750 GN=SAMN05444405_103191 PE=4 SV=1
MM1 pKa = 6.73 EE2 pKa = 4.7 TAPNKK7 pKa = 10.47 LIVVSYY13 pKa = 9.67 EE14 pKa = 3.95 LYY16 pKa = 8.92 VTEE19 pKa = 5.18 DD20 pKa = 3.59 GEE22 pKa = 4.41 RR23 pKa = 11.84 DD24 pKa = 3.67 LVEE27 pKa = 4.39 KK28 pKa = 9.54 ATPEE32 pKa = 3.79 QPFQFISGLGTTLDD46 pKa = 3.55 AFEE49 pKa = 4.54 SQLTGLAVGDD59 pKa = 3.78 KK60 pKa = 11.06 FEE62 pKa = 4.3 FTISSTEE69 pKa = 3.56 AYY71 pKa = 10.0 GDD73 pKa = 3.72 YY74 pKa = 10.99 NEE76 pKa = 4.55 EE77 pKa = 4.16 HH78 pKa = 7.13 VIDD81 pKa = 4.04 LPKK84 pKa = 10.87 NIFEE88 pKa = 4.05 IDD90 pKa = 3.12 GRR92 pKa = 11.84 FDD94 pKa = 3.36 AEE96 pKa = 4.62 RR97 pKa = 11.84 IFAGNVVPLMDD108 pKa = 5.4 ADD110 pKa = 4.14 GNRR113 pKa = 11.84 MNATVVEE120 pKa = 4.44 VGNSNVKK127 pKa = 10.15 VDD129 pKa = 4.12 MNHH132 pKa = 6.71 PLAGEE137 pKa = 4.17 DD138 pKa = 3.29 LTFVGEE144 pKa = 4.24 VLEE147 pKa = 5.0 SRR149 pKa = 11.84 TATNEE154 pKa = 3.92 EE155 pKa = 3.84 IQGMINVMSGEE166 pKa = 4.3 GGCGCGCDD174 pKa = 4.63 SCGDD178 pKa = 3.58 DD179 pKa = 4.52 CGCDD183 pKa = 3.49 EE184 pKa = 4.94 EE185 pKa = 4.96 GHH187 pKa = 6.28 EE188 pKa = 4.88 GSCGSGCGCHH198 pKa = 6.4
Molecular weight: 21.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.961
IPC_protein 3.923
Toseland 3.732
ProMoST 4.037
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.77
Rodwell 3.745
Grimsley 3.643
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.151
Thurlkill 3.757
EMBOSS 3.783
Sillero 4.037
Patrickios 0.871
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A1M5GIJ4|A0A1M5GIJ4_9BACE DNA-binding protein HU-beta OS=Bacteroides luti OX=1297750 GN=SAMN05444405_12114 PE=3 SV=1
MM1 pKa = 7.91 PSGKK5 pKa = 9.66 KK6 pKa = 9.7 KK7 pKa = 9.82 KK8 pKa = 7.49 RR9 pKa = 11.84 HH10 pKa = 5.6 KK11 pKa = 10.32 MSTHH15 pKa = 5.04 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.87 KK19 pKa = 8.77 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.28 NRR25 pKa = 11.84 HH26 pKa = 4.94 KK27 pKa = 10.86 SKK29 pKa = 10.75 KK30 pKa = 9.6
Molecular weight: 3.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.965
IPC_protein 12.442
Toseland 12.647
ProMoST 13.1
Dawson 12.647
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.705
Grimsley 12.691
Solomon 13.115
Lehninger 13.027
Nozaki 12.647
DTASelect 12.618
Thurlkill 12.647
EMBOSS 13.13
Sillero 12.647
Patrickios 12.427
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.937
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3225
0
3225
1209947
24
2459
375.2
42.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.626 ± 0.044
1.166 ± 0.014
5.433 ± 0.028
6.226 ± 0.04
4.793 ± 0.031
6.66 ± 0.034
1.678 ± 0.014
7.506 ± 0.039
7.604 ± 0.035
8.995 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.572 ± 0.02
5.878 ± 0.037
3.559 ± 0.021
3.211 ± 0.022
3.845 ± 0.024
6.741 ± 0.031
5.529 ± 0.033
6.269 ± 0.032
1.223 ± 0.018
4.485 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here