Amino acid dipepetide frequency for Mucilaginibacter sp. OK283

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.156AlaAla: 7.156 ± 0.075
0.683AlaCys: 0.683 ± 0.018
5.075AlaAsp: 5.075 ± 0.053
4.079AlaGlu: 4.079 ± 0.052
3.628AlaPhe: 3.628 ± 0.047
6.308AlaGly: 6.308 ± 0.061
1.279AlaHis: 1.279 ± 0.026
5.729AlaIle: 5.729 ± 0.058
4.796AlaLys: 4.796 ± 0.055
7.053AlaLeu: 7.053 ± 0.065
1.79AlaMet: 1.79 ± 0.028
4.153AlaAsn: 4.153 ± 0.043
2.605AlaPro: 2.605 ± 0.039
3.173AlaGln: 3.173 ± 0.041
2.639AlaArg: 2.639 ± 0.039
4.656AlaSer: 4.656 ± 0.056
4.585AlaThr: 4.585 ± 0.068
5.091AlaVal: 5.091 ± 0.058
0.868AlaTrp: 0.868 ± 0.022
3.134AlaTyr: 3.134 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.517CysAla: 0.517 ± 0.016
0.138CysCys: 0.138 ± 0.008
0.377CysAsp: 0.377 ± 0.014
0.331CysGlu: 0.331 ± 0.014
0.47CysPhe: 0.47 ± 0.016
0.649CysGly: 0.649 ± 0.018
0.187CysHis: 0.187 ± 0.011
0.615CysIle: 0.615 ± 0.017
0.52CysLys: 0.52 ± 0.016
0.817CysLeu: 0.817 ± 0.021
0.192CysMet: 0.192 ± 0.011
0.381CysAsn: 0.381 ± 0.014
0.308CysPro: 0.308 ± 0.012
0.243CysGln: 0.243 ± 0.01
0.33CysArg: 0.33 ± 0.012
0.509CysSer: 0.509 ± 0.016
0.452CysThr: 0.452 ± 0.015
0.441CysVal: 0.441 ± 0.015
0.099CysTrp: 0.099 ± 0.006
0.325CysTyr: 0.325 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
4.332AspAla: 4.332 ± 0.049
0.386AspCys: 0.386 ± 0.013
2.986AspAsp: 2.986 ± 0.046
3.351AspGlu: 3.351 ± 0.05
3.036AspPhe: 3.036 ± 0.042
4.193AspGly: 4.193 ± 0.054
1.067AspHis: 1.067 ± 0.026
3.988AspIle: 3.988 ± 0.042
4.078AspLys: 4.078 ± 0.047
4.807AspLeu: 4.807 ± 0.052
1.208AspMet: 1.208 ± 0.027
2.972AspAsn: 2.972 ± 0.042
2.12AspPro: 2.12 ± 0.033
1.78AspGln: 1.78 ± 0.03
1.986AspArg: 1.986 ± 0.035
2.985AspSer: 2.985 ± 0.049
2.813AspThr: 2.813 ± 0.039
3.656AspVal: 3.656 ± 0.047
0.8AspTrp: 0.8 ± 0.022
2.574AspTyr: 2.574 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
3.941GluAla: 3.941 ± 0.061
0.295GluCys: 0.295 ± 0.012
2.413GluAsp: 2.413 ± 0.041
2.663GluGlu: 2.663 ± 0.054
2.199GluPhe: 2.199 ± 0.032
3.061GluGly: 3.061 ± 0.046
1.008GluHis: 1.008 ± 0.024
3.705GluIle: 3.705 ± 0.049
3.931GluLys: 3.931 ± 0.063
5.171GluLeu: 5.171 ± 0.07
1.297GluMet: 1.297 ± 0.029
2.76GluAsn: 2.76 ± 0.044
1.582GluPro: 1.582 ± 0.026
2.146GluGln: 2.146 ± 0.033
2.162GluArg: 2.162 ± 0.041
2.426GluSer: 2.426 ± 0.034
2.713GluThr: 2.713 ± 0.04
3.347GluVal: 3.347 ± 0.047
0.62GluTrp: 0.62 ± 0.018
1.924GluTyr: 1.924 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
3.611PheAla: 3.611 ± 0.042
0.5PheCys: 0.5 ± 0.016
3.013PheAsp: 3.013 ± 0.038
2.47PheGlu: 2.47 ± 0.039
2.439PhePhe: 2.439 ± 0.04
3.452PheGly: 3.452 ± 0.042
0.835PheHis: 0.835 ± 0.02
3.605PheIle: 3.605 ± 0.05
3.469PheLys: 3.469 ± 0.042
4.058PheLeu: 4.058 ± 0.051
1.128PheMet: 1.128 ± 0.023
3.204PheAsn: 3.204 ± 0.041
1.702PhePro: 1.702 ± 0.03
1.242PheGln: 1.242 ± 0.025
1.723PheArg: 1.723 ± 0.028
3.411PheSer: 3.411 ± 0.039
3.352PheThr: 3.352 ± 0.043
2.823PheVal: 2.823 ± 0.034
0.598PheTrp: 0.598 ± 0.017
2.175PheTyr: 2.175 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
4.91GlyAla: 4.91 ± 0.059
0.607GlyCys: 0.607 ± 0.019
3.628GlyAsp: 3.628 ± 0.038
3.052GlyGlu: 3.052 ± 0.042
3.734GlyPhe: 3.734 ± 0.048
5.181GlyGly: 5.181 ± 0.071
1.344GlyHis: 1.344 ± 0.027
5.444GlyIle: 5.444 ± 0.054
5.329GlyLys: 5.329 ± 0.05
6.56GlyLeu: 6.56 ± 0.065
1.66GlyMet: 1.66 ± 0.032
3.987GlyAsn: 3.987 ± 0.059
1.826GlyPro: 1.826 ± 0.034
2.382GlyGln: 2.382 ± 0.034
2.63GlyArg: 2.63 ± 0.036
4.586GlySer: 4.586 ± 0.056
4.499GlyThr: 4.499 ± 0.071
4.51GlyVal: 4.51 ± 0.058
1.079GlyTrp: 1.079 ± 0.022
3.332GlyTyr: 3.332 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
1.189HisAla: 1.189 ± 0.025
0.179HisCys: 0.179 ± 0.009
1.036HisAsp: 1.036 ± 0.021
0.959HisGlu: 0.959 ± 0.022
1.158HisPhe: 1.158 ± 0.023
1.186HisGly: 1.186 ± 0.027
0.55HisHis: 0.55 ± 0.02
1.424HisIle: 1.424 ± 0.03
1.049HisLys: 1.049 ± 0.024
1.824HisLeu: 1.824 ± 0.031
0.379HisMet: 0.379 ± 0.013
1.016HisAsn: 1.016 ± 0.023
1.041HisPro: 1.041 ± 0.026
0.785HisGln: 0.785 ± 0.019
0.757HisArg: 0.757 ± 0.02
1.045HisSer: 1.045 ± 0.022
1.033HisThr: 1.033 ± 0.025
1.05HisVal: 1.05 ± 0.021
0.278HisTrp: 0.278 ± 0.012
0.891HisTyr: 0.891 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.073IleAla: 6.073 ± 0.069
0.695IleCys: 0.695 ± 0.018
4.219IleAsp: 4.219 ± 0.043
3.681IleGlu: 3.681 ± 0.052
3.098IlePhe: 3.098 ± 0.042
4.81IleGly: 4.81 ± 0.058
1.242IleHis: 1.242 ± 0.025
5.151IleIle: 5.151 ± 0.065
5.177IleLys: 5.177 ± 0.051
5.89IleLeu: 5.89 ± 0.057
1.34IleMet: 1.34 ± 0.03
4.553IleAsn: 4.553 ± 0.057
3.055IlePro: 3.055 ± 0.04
2.219IleGln: 2.219 ± 0.034
2.841IleArg: 2.841 ± 0.04
5.038IleSer: 5.038 ± 0.051
4.934IleThr: 4.934 ± 0.056
4.229IleVal: 4.229 ± 0.056
0.734IleTrp: 0.734 ± 0.021
2.801IleTyr: 2.801 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
5.507LysAla: 5.507 ± 0.056
0.318LysCys: 0.318 ± 0.013
3.942LysAsp: 3.942 ± 0.049
3.717LysGlu: 3.717 ± 0.056
2.655LysPhe: 2.655 ± 0.036
4.545LysGly: 4.545 ± 0.052
1.313LysHis: 1.313 ± 0.03
4.858LysIle: 4.858 ± 0.052
5.256LysLys: 5.256 ± 0.067
6.391LysLeu: 6.391 ± 0.053
1.788LysMet: 1.788 ± 0.029
4.243LysAsn: 4.243 ± 0.048
2.976LysPro: 2.976 ± 0.041
3.007LysGln: 3.007 ± 0.046
2.585LysArg: 2.585 ± 0.041
3.821LysSer: 3.821 ± 0.041
4.443LysThr: 4.443 ± 0.048
4.434LysVal: 4.434 ± 0.045
0.79LysTrp: 0.79 ± 0.022
2.949LysTyr: 2.949 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
7.086LeuAla: 7.086 ± 0.064
0.787LeuCys: 0.787 ± 0.021
4.651LeuAsp: 4.651 ± 0.053
4.019LeuGlu: 4.019 ± 0.057
4.651LeuPhe: 4.651 ± 0.05
5.65LeuGly: 5.65 ± 0.057
1.673LeuHis: 1.673 ± 0.03
6.427LeuIle: 6.427 ± 0.068
7.217LeuLys: 7.217 ± 0.068
9.361LeuLeu: 9.361 ± 0.096
2.204LeuMet: 2.204 ± 0.031
5.677LeuAsn: 5.677 ± 0.064
4.27LeuPro: 4.27 ± 0.048
3.568LeuGln: 3.568 ± 0.044
3.403LeuArg: 3.403 ± 0.043
6.678LeuSer: 6.678 ± 0.065
5.693LeuThr: 5.693 ± 0.061
5.618LeuVal: 5.618 ± 0.055
1.03LeuTrp: 1.03 ± 0.024
3.354LeuTyr: 3.354 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
1.957MetAla: 1.957 ± 0.034
0.151MetCys: 0.151 ± 0.009
1.204MetAsp: 1.204 ± 0.027
1.242MetGlu: 1.242 ± 0.029
0.838MetPhe: 0.838 ± 0.023
1.515MetGly: 1.515 ± 0.032
0.445MetHis: 0.445 ± 0.015
1.47MetIle: 1.47 ± 0.025
1.887MetLys: 1.887 ± 0.031
2.125MetLeu: 2.125 ± 0.036
0.569MetMet: 0.569 ± 0.017
1.178MetAsn: 1.178 ± 0.023
1.168MetPro: 1.168 ± 0.023
0.993MetGln: 0.993 ± 0.024
0.92MetArg: 0.92 ± 0.023
1.255MetSer: 1.255 ± 0.025
1.068MetThr: 1.068 ± 0.024
1.537MetVal: 1.537 ± 0.033
0.179MetTrp: 0.179 ± 0.009
0.679MetTyr: 0.679 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
4.234AsnAla: 4.234 ± 0.051
0.415AsnCys: 0.415 ± 0.014
3.018AsnAsp: 3.018 ± 0.04
2.669AsnGlu: 2.669 ± 0.037
2.691AsnPhe: 2.691 ± 0.04
4.366AsnGly: 4.366 ± 0.055
1.11AsnHis: 1.11 ± 0.029
4.322AsnIle: 4.322 ± 0.051
3.744AsnLys: 3.744 ± 0.039
5.126AsnLeu: 5.126 ± 0.061
1.23AsnMet: 1.23 ± 0.023
3.592AsnAsn: 3.592 ± 0.051
2.777AsnPro: 2.777 ± 0.036
2.216AsnGln: 2.216 ± 0.036
2.19AsnArg: 2.19 ± 0.035
3.495AsnSer: 3.495 ± 0.051
3.536AsnThr: 3.536 ± 0.049
3.408AsnVal: 3.408 ± 0.051
0.817AsnTrp: 0.817 ± 0.02
2.817AsnTyr: 2.817 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
3.753ProAla: 3.753 ± 0.051
0.22ProCys: 0.22 ± 0.01
2.729ProAsp: 2.729 ± 0.039
2.582ProGlu: 2.582 ± 0.04
1.923ProPhe: 1.923 ± 0.037
3.301ProGly: 3.301 ± 0.05
0.708ProHis: 0.708 ± 0.019
2.335ProIle: 2.335 ± 0.034
2.217ProLys: 2.217 ± 0.036
3.404ProLeu: 3.404 ± 0.041
0.756ProMet: 0.756 ± 0.019
2.01ProAsn: 2.01 ± 0.035
1.258ProPro: 1.258 ± 0.031
1.56ProGln: 1.56 ± 0.027
1.144ProArg: 1.144 ± 0.023
2.122ProSer: 2.122 ± 0.037
2.012ProThr: 2.012 ± 0.035
3.557ProVal: 3.557 ± 0.056
0.476ProTrp: 0.476 ± 0.017
1.643ProTyr: 1.643 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
2.786GlnAla: 2.786 ± 0.043
0.209GlnCys: 0.209 ± 0.01
1.622GlnAsp: 1.622 ± 0.028
1.805GlnGlu: 1.805 ± 0.036
1.653GlnPhe: 1.653 ± 0.031
2.075GlnGly: 2.075 ± 0.031
0.797GlnHis: 0.797 ± 0.02
2.419GlnIle: 2.419 ± 0.037
2.704GlnLys: 2.704 ± 0.037
3.923GlnLeu: 3.923 ± 0.047
0.912GlnMet: 0.912 ± 0.022
2.158GlnAsn: 2.158 ± 0.04
1.578GlnPro: 1.578 ± 0.031
2.177GlnGln: 2.177 ± 0.058
1.462GlnArg: 1.462 ± 0.03
2.31GlnSer: 2.31 ± 0.033
2.296GlnThr: 2.296 ± 0.037
2.563GlnVal: 2.563 ± 0.036
0.5GlnTrp: 0.5 ± 0.018
1.608GlnTyr: 1.608 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
2.5ArgAla: 2.5 ± 0.039
0.242ArgCys: 0.242 ± 0.01
1.978ArgAsp: 1.978 ± 0.036
1.988ArgGlu: 1.988 ± 0.035
2.062ArgPhe: 2.062 ± 0.03
2.156ArgGly: 2.156 ± 0.035
0.74ArgHis: 0.74 ± 0.02
2.783ArgIle: 2.783 ± 0.038
2.598ArgLys: 2.598 ± 0.035
3.787ArgLeu: 3.787 ± 0.05
0.98ArgMet: 0.98 ± 0.02
2.098ArgAsn: 2.098 ± 0.032
1.375ArgPro: 1.375 ± 0.029
1.543ArgGln: 1.543 ± 0.032
1.504ArgArg: 1.504 ± 0.026
2.162ArgSer: 2.162 ± 0.033
1.938ArgThr: 1.938 ± 0.031
2.4ArgVal: 2.4 ± 0.032
0.516ArgTrp: 0.516 ± 0.017
1.834ArgTyr: 1.834 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
4.983SerAla: 4.983 ± 0.06
0.578SerCys: 0.578 ± 0.017
2.924SerAsp: 2.924 ± 0.033
2.574SerGlu: 2.574 ± 0.041
3.485SerPhe: 3.485 ± 0.042
4.917SerGly: 4.917 ± 0.065
1.158SerHis: 1.158 ± 0.025
4.62SerIle: 4.62 ± 0.056
3.852SerLys: 3.852 ± 0.048
5.938SerLeu: 5.938 ± 0.051
1.236SerMet: 1.236 ± 0.025
3.257SerAsn: 3.257 ± 0.052
2.604SerPro: 2.604 ± 0.041
2.01SerGln: 2.01 ± 0.033
2.356SerArg: 2.356 ± 0.033
3.936SerSer: 3.936 ± 0.059
3.621SerThr: 3.621 ± 0.05
4.25SerVal: 4.25 ± 0.052
0.811SerTrp: 0.811 ± 0.021
2.801SerTyr: 2.801 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
5.145ThrAla: 5.145 ± 0.064
0.418ThrCys: 0.418 ± 0.015
3.703ThrAsp: 3.703 ± 0.045
2.777ThrGlu: 2.777 ± 0.04
2.838ThrPhe: 2.838 ± 0.039
5.328ThrGly: 5.328 ± 0.069
1.064ThrHis: 1.064 ± 0.023
4.507ThrIle: 4.507 ± 0.05
3.174ThrLys: 3.174 ± 0.037
5.458ThrLeu: 5.458 ± 0.057
1.086ThrMet: 1.086 ± 0.024
3.138ThrAsn: 3.138 ± 0.045
2.733ThrPro: 2.733 ± 0.043
2.037ThrGln: 2.037 ± 0.03
2.038ThrArg: 2.038 ± 0.033
3.612ThrSer: 3.612 ± 0.057
3.841ThrThr: 3.841 ± 0.062
4.217ThrVal: 4.217 ± 0.057
0.766ThrTrp: 0.766 ± 0.023
2.665ThrTyr: 2.665 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
4.945ValAla: 4.945 ± 0.051
0.587ValCys: 0.587 ± 0.016
3.484ValAsp: 3.484 ± 0.044
3.002ValGlu: 3.002 ± 0.042
3.279ValPhe: 3.279 ± 0.039
3.77ValGly: 3.77 ± 0.049
1.093ValHis: 1.093 ± 0.026
4.906ValIle: 4.906 ± 0.053
4.782ValLys: 4.782 ± 0.054
6.063ValLeu: 6.063 ± 0.058
1.465ValMet: 1.465 ± 0.032
3.943ValAsn: 3.943 ± 0.048
2.531ValPro: 2.531 ± 0.035
2.074ValGln: 2.074 ± 0.033
2.136ValArg: 2.136 ± 0.035
4.43ValSer: 4.43 ± 0.052
4.314ValThr: 4.314 ± 0.066
4.315ValVal: 4.315 ± 0.052
0.743ValTrp: 0.743 ± 0.021
2.676ValTyr: 2.676 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.882TrpAla: 0.882 ± 0.022
0.121TrpCys: 0.121 ± 0.008
0.743TrpAsp: 0.743 ± 0.023
0.602TrpGlu: 0.602 ± 0.017
0.642TrpPhe: 0.642 ± 0.019
0.894TrpGly: 0.894 ± 0.025
0.271TrpHis: 0.271 ± 0.011
0.801TrpIle: 0.801 ± 0.021
0.856TrpLys: 0.856 ± 0.021
1.233TrpLeu: 1.233 ± 0.024
0.365TrpMet: 0.365 ± 0.013
0.708TrpAsn: 0.708 ± 0.018
0.413TrpPro: 0.413 ± 0.017
0.603TrpGln: 0.603 ± 0.017
0.514TrpArg: 0.514 ± 0.016
0.682TrpSer: 0.682 ± 0.02
0.679TrpThr: 0.679 ± 0.018
0.792TrpVal: 0.792 ± 0.018
0.234TrpTrp: 0.234 ± 0.011
0.48TrpTyr: 0.48 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.974TyrAla: 2.974 ± 0.041
0.373TyrCys: 0.373 ± 0.013
2.373TyrAsp: 2.373 ± 0.043
1.802TyrGlu: 1.802 ± 0.029
2.33TyrPhe: 2.33 ± 0.034
2.921TyrGly: 2.921 ± 0.043
0.945TyrHis: 0.945 ± 0.023
2.669TyrIle: 2.669 ± 0.037
2.901TyrLys: 2.901 ± 0.039
4.028TyrLeu: 4.028 ± 0.048
0.794TyrMet: 0.794 ± 0.017
2.745TyrAsn: 2.745 ± 0.048
1.79TyrPro: 1.79 ± 0.028
1.8TyrGln: 1.8 ± 0.032
1.862TyrArg: 1.862 ± 0.031
2.753TyrSer: 2.753 ± 0.041
2.723TyrThr: 2.723 ± 0.04
2.301TyrVal: 2.301 ± 0.037
0.58TyrTrp: 0.58 ± 0.015
2.1TyrTyr: 2.1 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5961 proteins (2089980 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski