Wuhan Insect virus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Wuhan 5 insect cytorhabdovirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KTM4|A0A0B5KTM4_9RHAB 4b protein OS=Wuhan Insect virus 5 OX=1608110 GN=4b PE=4 SV=1
MM1 pKa = 7.26VDD3 pKa = 3.21KK4 pKa = 11.11VPNEE8 pKa = 3.59FAGAFNPDD16 pKa = 2.83GKK18 pKa = 9.37TDD20 pKa = 4.39FSSLADD26 pKa = 3.79NLDD29 pKa = 3.87DD30 pKa = 5.1SVDD33 pKa = 4.13NIPDD37 pKa = 3.96PSLSIPTQARR47 pKa = 11.84EE48 pKa = 4.45AVDD51 pKa = 3.64QPRR54 pKa = 11.84KK55 pKa = 9.95QPVGDD60 pKa = 3.66VAATLLCLKK69 pKa = 9.02NTAHH73 pKa = 6.0NHH75 pKa = 4.55GAIVTNDD82 pKa = 3.15MEE84 pKa = 5.28SLFKK88 pKa = 10.57YY89 pKa = 10.24YY90 pKa = 10.35CANEE94 pKa = 3.84QLDD97 pKa = 3.42IRR99 pKa = 11.84DD100 pKa = 2.91IEE102 pKa = 4.43FFVRR106 pKa = 11.84GYY108 pKa = 11.11VYY110 pKa = 10.25ATGSQVGPKK119 pKa = 9.4IQEE122 pKa = 3.76ATEE125 pKa = 4.05GLKK128 pKa = 10.45VEE130 pKa = 4.05MRR132 pKa = 11.84AIQRR136 pKa = 11.84ANATLAEE143 pKa = 4.45TIKK146 pKa = 11.04LLANQAVSVEE156 pKa = 4.15KK157 pKa = 10.47EE158 pKa = 3.53IAALSVNVKK167 pKa = 10.58NDD169 pKa = 2.8VTAALKK175 pKa = 10.79SAVAGKK181 pKa = 10.37AKK183 pKa = 10.67LGDD186 pKa = 3.77QPVSPLQTVKK196 pKa = 10.98LPLAKK201 pKa = 10.49ASLEE205 pKa = 4.2VVAAPNPVPATLEE218 pKa = 3.76VGEE221 pKa = 4.23PSKK224 pKa = 10.83PSEE227 pKa = 4.46TGLSDD232 pKa = 3.92PSQLKK237 pKa = 10.28KK238 pKa = 9.82MRR240 pKa = 11.84SVLSMIGVEE249 pKa = 4.73DD250 pKa = 3.56AVLNNLLDD258 pKa = 3.61NDD260 pKa = 3.84IPVIYY265 pKa = 9.02PAAIMEE271 pKa = 4.53EE272 pKa = 4.29YY273 pKa = 11.05GNLLGDD279 pKa = 4.44PEE281 pKa = 4.45VCDD284 pKa = 4.55LIKK287 pKa = 10.9EE288 pKa = 4.18EE289 pKa = 4.19VMGNIEE295 pKa = 4.95KK296 pKa = 10.59FLLCGAA302 pKa = 4.91

Molecular weight: 32.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KXN2|A0A0B5KXN2_9RHAB Matrix protein OS=Wuhan Insect virus 5 OX=1608110 GN=M PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 4.47SQMVVSSGEE11 pKa = 3.91FTMGEE16 pKa = 3.93TDD18 pKa = 4.29GVISLSKK25 pKa = 10.64KK26 pKa = 9.63IGLFQKK32 pKa = 10.81LLTKK36 pKa = 10.8FGYY39 pKa = 9.39TNIAVHH45 pKa = 6.12QVIFKK50 pKa = 10.43YY51 pKa = 10.37KK52 pKa = 9.3SRR54 pKa = 11.84CPAGAEE60 pKa = 3.96GLMTLKK66 pKa = 10.26MVDD69 pKa = 3.81RR70 pKa = 11.84RR71 pKa = 11.84LDD73 pKa = 3.89DD74 pKa = 5.12PDD76 pKa = 6.34DD77 pKa = 3.83MMIDD81 pKa = 3.55GLSFDD86 pKa = 3.76VKK88 pKa = 10.91DD89 pKa = 3.66WIVFSWSYY97 pKa = 10.54PCWFHH102 pKa = 7.66YY103 pKa = 10.69KK104 pKa = 10.54DD105 pKa = 4.13FEE107 pKa = 4.67SKK109 pKa = 10.73EE110 pKa = 3.95KK111 pKa = 10.8DD112 pKa = 3.33LLEE115 pKa = 4.49IEE117 pKa = 4.37WGVSGSNMIDD127 pKa = 3.51SVSLGHH133 pKa = 5.96YY134 pKa = 8.69KK135 pKa = 10.57VKK137 pKa = 10.2IAYY140 pKa = 9.5KK141 pKa = 8.9MRR143 pKa = 11.84NDD145 pKa = 2.89ITRR148 pKa = 11.84LVSNPNIAQKK158 pKa = 10.29VHH160 pKa = 5.8SQVHH164 pKa = 4.04VSKK167 pKa = 10.5FVKK170 pKa = 9.7KK171 pKa = 10.35QKK173 pKa = 9.26TGRR176 pKa = 11.84LSKK179 pKa = 10.19STGNLLNVSSPPDD192 pKa = 3.27SGMKK196 pKa = 8.08NTKK199 pKa = 9.95NSSDD203 pKa = 3.53YY204 pKa = 10.88RR205 pKa = 11.84KK206 pKa = 10.45EE207 pKa = 3.87EE208 pKa = 4.1LLTVSAEE215 pKa = 3.82RR216 pKa = 11.84LFPIRR221 pKa = 11.84GVKK224 pKa = 8.97PP225 pKa = 3.34

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3814

187

2098

635.7

71.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.821 ± 1.454

2.071 ± 0.352

5.952 ± 0.211

5.69 ± 0.219

3.644 ± 0.279

5.821 ± 0.346

2.045 ± 0.29

6.319 ± 0.517

6.266 ± 0.782

9.255 ± 0.606

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.644 ± 0.402

3.723 ± 0.325

4.51 ± 0.462

2.989 ± 0.646

5.48 ± 1.165

9.491 ± 0.469

5.506 ± 0.31

6.738 ± 0.5

1.363 ± 0.261

3.671 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski