Hubei tombus-like virus 16

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG84|A0A1L3KG84_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 16 OX=1923262 PE=4 SV=1
MM1 pKa = 7.15FVKK4 pKa = 10.04PDD6 pKa = 3.75KK7 pKa = 10.94IEE9 pKa = 4.18AGEE12 pKa = 4.23IQDD15 pKa = 3.69KK16 pKa = 10.59APRR19 pKa = 11.84AIQYY23 pKa = 8.9RR24 pKa = 11.84GPAYY28 pKa = 9.82NLEE31 pKa = 4.07MLRR34 pKa = 11.84YY35 pKa = 9.34IKK37 pKa = 10.46PFEE40 pKa = 4.09HH41 pKa = 7.1KK42 pKa = 10.29IYY44 pKa = 10.92DD45 pKa = 3.83EE46 pKa = 4.21LHH48 pKa = 6.16LGNYY52 pKa = 9.1SRR54 pKa = 11.84TRR56 pKa = 11.84AIVKK60 pKa = 9.61GLNNYY65 pKa = 7.99QRR67 pKa = 11.84AEE69 pKa = 3.95LFFEE73 pKa = 4.38KK74 pKa = 10.31LQNFQQPAFLLIDD87 pKa = 3.46HH88 pKa = 6.92SKK90 pKa = 10.17FDD92 pKa = 3.34STVRR96 pKa = 11.84VEE98 pKa = 4.47HH99 pKa = 6.44LRR101 pKa = 11.84STHH104 pKa = 5.37RR105 pKa = 11.84KK106 pKa = 7.89YY107 pKa = 10.57MKK109 pKa = 10.48AFNSKK114 pKa = 10.13HH115 pKa = 5.62LWQLCKK121 pKa = 10.74VQISNKK127 pKa = 9.33CYY129 pKa = 9.57SKK131 pKa = 11.25NGIHH135 pKa = 5.03YY136 pKa = 6.93TSRR139 pKa = 11.84GTRR142 pKa = 11.84MSGDD146 pKa = 3.84PDD148 pKa = 3.89TGCGNSVINGDD159 pKa = 4.12CLHH162 pKa = 6.46EE163 pKa = 4.17VLRR166 pKa = 11.84VSGIAKK172 pKa = 9.95YY173 pKa = 10.62EE174 pKa = 3.83IMLDD178 pKa = 3.65GDD180 pKa = 3.89DD181 pKa = 4.67SIVIIEE187 pKa = 4.53KK188 pKa = 10.35GALSAFKK195 pKa = 10.22YY196 pKa = 10.72DD197 pKa = 2.96HH198 pKa = 6.76FEE200 pKa = 3.88RR201 pKa = 11.84LGFEE205 pKa = 4.18TKK207 pKa = 9.72MSVVYY212 pKa = 10.05DD213 pKa = 3.34YY214 pKa = 11.68HH215 pKa = 7.71EE216 pKa = 4.48VEE218 pKa = 4.83FCRR221 pKa = 11.84SKK223 pKa = 11.33PMMHH227 pKa = 7.07PRR229 pKa = 11.84PCFIRR234 pKa = 11.84DD235 pKa = 3.62PKK237 pKa = 10.12RR238 pKa = 11.84TISNTMMCLKK248 pKa = 10.38HH249 pKa = 5.72YY250 pKa = 10.83AEE252 pKa = 5.16RR253 pKa = 11.84DD254 pKa = 3.74YY255 pKa = 11.43KK256 pKa = 10.97SWLAAVGLCEE266 pKa = 4.33LACNDD271 pKa = 3.87GVPVISVLGRR281 pKa = 11.84TLSGFSKK288 pKa = 10.55RR289 pKa = 11.84KK290 pKa = 10.12LFDD293 pKa = 4.38EE294 pKa = 4.3DD295 pKa = 4.48TLWKK299 pKa = 9.54MGNLAGGKK307 pKa = 9.47YY308 pKa = 7.49DD309 pKa = 3.7TPITTEE315 pKa = 3.54SRR317 pKa = 11.84LEE319 pKa = 3.86YY320 pKa = 11.1AMTWGVDD327 pKa = 3.62CEE329 pKa = 4.36VQLLLEE335 pKa = 4.59EE336 pKa = 4.26EE337 pKa = 4.71FKK339 pKa = 10.97TSIDD343 pKa = 4.81DD344 pKa = 3.56SFHH347 pKa = 5.0YY348 pKa = 9.98CKK350 pKa = 10.58RR351 pKa = 11.84IPRR354 pKa = 11.84VKK356 pKa = 10.14QRR358 pKa = 11.84TNIKK362 pKa = 8.16STRR365 pKa = 11.84EE366 pKa = 3.84NFNGRR371 pKa = 11.84TTVSVWTAASAYY383 pKa = 9.99QSLDD387 pKa = 3.36QFSSSSWWCSGG398 pKa = 3.02

Molecular weight: 46.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGL2|A0A1L3KGL2_9VIRU Capsid protein OS=Hubei tombus-like virus 16 OX=1923262 PE=3 SV=1
MM1 pKa = 7.79AGQLYY6 pKa = 9.33PYY8 pKa = 8.31GQPRR12 pKa = 11.84PRR14 pKa = 11.84INPWINLAAAAGGAAARR31 pKa = 11.84QLVNYY36 pKa = 8.11GANQFNAYY44 pKa = 8.5MRR46 pKa = 11.84GPPPPPRR53 pKa = 11.84GGRR56 pKa = 11.84RR57 pKa = 11.84GRR59 pKa = 11.84GRR61 pKa = 11.84RR62 pKa = 11.84SFNMQPMLAALPPPPPRR79 pKa = 11.84ARR81 pKa = 11.84RR82 pKa = 11.84GGRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84TARR91 pKa = 11.84GSNAGGSPTQIFRR104 pKa = 11.84GTEE107 pKa = 3.77YY108 pKa = 10.66LAEE111 pKa = 4.36PTTTLATLQFNCASTTHH128 pKa = 6.42PRR130 pKa = 11.84LSNTAKK136 pKa = 9.96CYY138 pKa = 8.53EE139 pKa = 4.24RR140 pKa = 11.84YY141 pKa = 9.42RR142 pKa = 11.84VRR144 pKa = 11.84SVVLTYY150 pKa = 11.19NSTSGTATTGGVTLGIHH167 pKa = 6.74PGPTNSNVKK176 pKa = 10.02KK177 pKa = 10.88AEE179 pKa = 5.12DD180 pKa = 3.54ILKK183 pKa = 9.98LDD185 pKa = 4.17PSRR188 pKa = 11.84TTPAWKK194 pKa = 10.37NSTIRR199 pKa = 11.84LGQNIDD205 pKa = 3.13AQRR208 pKa = 11.84WMHH211 pKa = 5.75VGKK214 pKa = 7.67TTEE217 pKa = 3.9EE218 pKa = 4.4GVAFTCYY225 pKa = 10.09YY226 pKa = 9.76IKK228 pKa = 11.0SGDD231 pKa = 3.29ATGQIKK237 pKa = 8.25ITYY240 pKa = 8.9DD241 pKa = 2.83IEE243 pKa = 3.91FAYY246 pKa = 9.28PVPFF250 pKa = 4.67

Molecular weight: 27.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1146

250

498

382.0

43.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.981 ± 1.116

1.832 ± 0.525

4.538 ± 0.788

6.457 ± 1.202

4.101 ± 0.41

5.934 ± 1.443

2.443 ± 0.433

4.974 ± 0.256

6.457 ± 0.954

7.941 ± 0.779

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.336

4.45 ± 0.34

7.853 ± 2.06

3.578 ± 0.25

8.115 ± 0.815

5.41 ± 1.209

5.846 ± 1.237

5.236 ± 0.513

1.571 ± 0.116

4.014 ± 0.523
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski