Alkalicoccus saliphilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalicoccus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3323 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4U5H2|A0A2T4U5H2_9BACI Uncharacterized protein OS=Alkalicoccus saliphilus OX=200989 GN=C6Y45_09895 PE=4 SV=1
MM1 pKa = 7.3YY2 pKa = 10.37FLDD5 pKa = 5.41LEE7 pKa = 5.09DD8 pKa = 4.52GSEE11 pKa = 4.03TRR13 pKa = 11.84DD14 pKa = 3.69PIEE17 pKa = 4.08IGNPIKK23 pKa = 10.92GSVSLDD29 pKa = 3.21ASGSPILYY37 pKa = 10.21VGDD40 pKa = 3.89GVPEE44 pKa = 4.16GEE46 pKa = 4.0PFGFRR51 pKa = 11.84LFDD54 pKa = 4.34LYY56 pKa = 11.15TNEE59 pKa = 4.36KK60 pKa = 10.5LHH62 pKa = 6.04FQTGRR67 pKa = 11.84DD68 pKa = 3.42DD69 pKa = 3.44YY70 pKa = 11.7AYY72 pKa = 10.44RR73 pKa = 11.84EE74 pKa = 3.96WAAFDD79 pKa = 3.46SSALFNRR86 pKa = 11.84EE87 pKa = 3.83DD88 pKa = 4.17DD89 pKa = 3.87VLLTGGEE96 pKa = 3.97NGLLYY101 pKa = 10.39NVEE104 pKa = 4.56MNTEE108 pKa = 3.93HH109 pKa = 7.36DD110 pKa = 4.15AEE112 pKa = 5.66DD113 pKa = 3.95GTVSVDD119 pKa = 3.62PEE121 pKa = 4.62VKK123 pKa = 9.02TSRR126 pKa = 11.84YY127 pKa = 9.04EE128 pKa = 4.23ADD130 pKa = 3.13DD131 pKa = 3.98HH132 pKa = 6.8EE133 pKa = 4.64YY134 pKa = 10.77QGIEE138 pKa = 4.07SSLAAYY144 pKa = 9.44KK145 pKa = 10.7NLTYY149 pKa = 10.31FADD152 pKa = 3.61NSGTITAYY160 pKa = 9.34DD161 pKa = 3.69TEE163 pKa = 4.63LNEE166 pKa = 4.58PVWMLPPLDD175 pKa = 4.7DD176 pKa = 3.5TDD178 pKa = 3.52ATIVLEE184 pKa = 4.06IEE186 pKa = 3.99EE187 pKa = 4.44GHH189 pKa = 6.77PYY191 pKa = 10.56LYY193 pKa = 10.35TGSEE197 pKa = 3.91VDD199 pKa = 3.67NVEE202 pKa = 4.06EE203 pKa = 5.5DD204 pKa = 3.31GTSYY208 pKa = 10.81LRR210 pKa = 11.84KK211 pKa = 9.11IDD213 pKa = 3.67GLTGEE218 pKa = 4.51EE219 pKa = 4.09IWRR222 pKa = 11.84EE223 pKa = 4.1GYY225 pKa = 9.76DD226 pKa = 2.94AYY228 pKa = 11.13YY229 pKa = 10.27YY230 pKa = 9.58PGVVGGVLATPVVGKK245 pKa = 10.45DD246 pKa = 4.5DD247 pKa = 4.22IDD249 pKa = 3.97DD250 pKa = 5.33LIIFTIARR258 pKa = 11.84TTDD261 pKa = 3.1VYY263 pKa = 11.24AGTMVALDD271 pKa = 3.94KK272 pKa = 10.58EE273 pKa = 4.7TGEE276 pKa = 4.67EE277 pKa = 4.14VWTWEE282 pKa = 3.89MEE284 pKa = 4.35DD285 pKa = 4.27YY286 pKa = 10.59GWSSPAPVYY295 pKa = 10.78QEE297 pKa = 5.42DD298 pKa = 4.58GPTYY302 pKa = 10.34LIQGDD307 pKa = 3.87WQGNLHH313 pKa = 6.6LLEE316 pKa = 4.83AATGEE321 pKa = 4.36KK322 pKa = 9.69KK323 pKa = 10.58DD324 pKa = 3.96EE325 pKa = 4.51LFLGANIEE333 pKa = 4.15ASPAVYY339 pKa = 10.39DD340 pKa = 3.72DD341 pKa = 4.36TIVLGTRR348 pKa = 11.84ADD350 pKa = 3.79TIFGIKK356 pKa = 9.57IKK358 pKa = 10.82

Molecular weight: 39.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4U2S9|A0A2T4U2S9_9BACI Serine/threonine protein phosphatase OS=Alkalicoccus saliphilus OX=200989 GN=C6Y45_14970 PE=4 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.57RR13 pKa = 11.84KK14 pKa = 7.65MNHH17 pKa = 4.64GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37NGRR29 pKa = 11.84KK30 pKa = 9.17VLANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.69GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3323

0

3323

966463

9

1529

290.8

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.941 ± 0.041

0.638 ± 0.01

5.216 ± 0.045

8.764 ± 0.052

4.418 ± 0.037

7.371 ± 0.036

2.166 ± 0.018

6.577 ± 0.044

5.925 ± 0.046

9.527 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.02

3.702 ± 0.033

3.915 ± 0.023

3.43 ± 0.029

4.702 ± 0.034

5.929 ± 0.029

5.388 ± 0.024

7.113 ± 0.033

1.06 ± 0.015

3.281 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski