Monosporascus sp. MC13-8B
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10637 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q4UQP1|A0A4Q4UQP1_9PEZI Uncharacterized protein OS=Monosporascus sp. MC13-8B OX=2211646 GN=DL766_007648 PE=4 SV=1
MM1 pKa = 7.86 HH2 pKa = 7.14 ITTILSLTILSALGAAEE19 pKa = 4.45 SNVDD23 pKa = 3.59 RR24 pKa = 11.84 DD25 pKa = 4.46 DD26 pKa = 4.05 VPSACLASCQSTIDD40 pKa = 3.44 LSQRR44 pKa = 11.84 CDD46 pKa = 3.21 RR47 pKa = 11.84 QTDD50 pKa = 3.19 DD51 pKa = 4.06 DD52 pKa = 4.82 NIYY55 pKa = 10.33 RR56 pKa = 11.84 GCVCGAQDD64 pKa = 4.37 AQRR67 pKa = 11.84 QMDD70 pKa = 3.35 QCAACVRR77 pKa = 11.84 VNGMSGPVDD86 pKa = 3.37 NDD88 pKa = 3.02 VAEE91 pKa = 4.39 LMNDD95 pKa = 3.75 CGWDD99 pKa = 3.5 FNTASGSATAVTSMTSRR116 pKa = 11.84 TTAGSTPTPVVSTSTGASTTATITQTPTGASGSGATEE153 pKa = 3.91 SSTTNSDD160 pKa = 3.06 NAAPAATAGLAGLLVAAGAAVALFF184 pKa = 3.95
Molecular weight: 18.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.63
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.872
Rodwell 3.681
Grimsley 3.541
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.304
Thurlkill 3.706
EMBOSS 3.872
Sillero 3.986
Patrickios 0.947
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|A0A4Q4W046|A0A4Q4W046_9PEZI Uncharacterized protein OS=Monosporascus sp. MC13-8B OX=2211646 GN=DL766_000428 PE=4 SV=1
MM1 pKa = 7.68 PLCGCKK7 pKa = 9.76 LHH9 pKa = 6.47 VYY11 pKa = 10.05 RR12 pKa = 11.84 FRR14 pKa = 11.84 LCRR17 pKa = 11.84 HH18 pKa = 5.57 YY19 pKa = 11.29 GNPIKK24 pKa = 10.55 HH25 pKa = 5.74 EE26 pKa = 4.2 CWRR29 pKa = 11.84 ADD31 pKa = 3.48 LRR33 pKa = 11.84 TKK35 pKa = 10.13 SPCMGSLFPCGKK47 pKa = 9.0 PVKK50 pKa = 9.84 AKK52 pKa = 10.22 HH53 pKa = 6.26 SRR55 pKa = 11.84 DD56 pKa = 3.77 GLCDD60 pKa = 2.91 SCAAYY65 pKa = 9.65 FAGFGDD71 pKa = 3.96 RR72 pKa = 11.84 SNEE75 pKa = 3.62 VLAEE79 pKa = 3.77 FLEE82 pKa = 4.6 YY83 pKa = 10.48 KK84 pKa = 10.41 EE85 pKa = 3.99 SRR87 pKa = 11.84 GWRR90 pKa = 11.84 KK91 pKa = 9.74 KK92 pKa = 10.09 RR93 pKa = 11.84 VNPRR97 pKa = 11.84 QVAPEE102 pKa = 3.97 KK103 pKa = 10.33 FVRR106 pKa = 11.84 PDD108 pKa = 3.12 ILLHH112 pKa = 5.78 NSSVRR117 pKa = 11.84 RR118 pKa = 11.84 GGAQPFRR125 pKa = 11.84 PQSPMRR131 pKa = 11.84 ATLALLPRR139 pKa = 11.84 NSHH142 pKa = 6.79 LPQPPPPVRR151 pKa = 11.84 QTQSGEE157 pKa = 3.49 RR158 pKa = 11.84 RR159 pKa = 11.84 AEE161 pKa = 3.65 RR162 pKa = 11.84 RR163 pKa = 11.84 ARR165 pKa = 11.84 TPFEE169 pKa = 3.76 HH170 pKa = 7.29 ALPGDD175 pKa = 4.06 CAASQSSSLILGDD188 pKa = 3.79 RR189 pKa = 11.84 LLMPNQGVYY198 pKa = 9.36 TLPRR202 pKa = 11.84 GHH204 pKa = 5.95 STEE207 pKa = 4.09 PCPLARR213 pKa = 11.84 RR214 pKa = 11.84 ITEE217 pKa = 3.61 EE218 pKa = 4.32 AEE220 pKa = 3.88 RR221 pKa = 11.84 LFPNPEE227 pKa = 3.56 SAMYY231 pKa = 9.92 PGVPKK236 pKa = 10.78 LSAAPKK242 pKa = 8.16 KK243 pKa = 9.88 HH244 pKa = 5.78 RR245 pKa = 11.84 RR246 pKa = 11.84 NHH248 pKa = 5.44 PRR250 pKa = 11.84 LPRR253 pKa = 11.84 GNDD256 pKa = 2.78 NKK258 pKa = 10.81 APEE261 pKa = 4.5 LTVVPAVIVSGGKK274 pKa = 9.68 AFKK277 pKa = 10.4 HH278 pKa = 5.78 RR279 pKa = 11.84 ADD281 pKa = 3.51 AATAATRR288 pKa = 11.84 SPSEE292 pKa = 4.25 PLPAPTPSRR301 pKa = 11.84 FFVLASPDD309 pKa = 3.28 AMSPTGCSAHH319 pKa = 5.73 VRR321 pKa = 11.84 SLSLDD326 pKa = 3.25 QTVAMPPPQRR336 pKa = 11.84 LEE338 pKa = 3.95 GVLAASCPQHH348 pKa = 6.55 GEE350 pKa = 4.1 RR351 pKa = 11.84 YY352 pKa = 10.26 DD353 pKa = 3.93 FGCGEE358 pKa = 4.17 CRR360 pKa = 11.84 GGLFRR365 pKa = 11.84 EE366 pKa = 4.22 RR367 pKa = 11.84 GLPTEE372 pKa = 4.23 EE373 pKa = 3.45 HH374 pKa = 6.33 RR375 pKa = 11.84 EE376 pKa = 4.24 RR377 pKa = 11.84 YY378 pKa = 9.77 RR379 pKa = 11.84 SMTPVSVAAAEE390 pKa = 4.21 TSNNRR395 pKa = 11.84 LGHH398 pKa = 5.91 CRR400 pKa = 11.84 STPLVVSVTTPRR412 pKa = 11.84 RR413 pKa = 11.84 SSPVPRR419 pKa = 11.84 KK420 pKa = 9.53 ACARR424 pKa = 11.84 AAPSRR429 pKa = 11.84 GTCVSPVTRR438 pKa = 11.84 RR439 pKa = 11.84 RR440 pKa = 11.84 PSRR443 pKa = 11.84 NRR445 pKa = 11.84 SRR447 pKa = 11.84 AVGSEE452 pKa = 4.0 DD453 pKa = 3.26
Molecular weight: 49.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 9.443
IPC_protein 10.145
Toseland 10.511
ProMoST 10.218
Dawson 10.613
Bjellqvist 10.365
Wikipedia 10.833
Rodwell 10.76
Grimsley 10.672
Solomon 10.73
Lehninger 10.701
Nozaki 10.57
DTASelect 10.335
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.438
IPC_peptide 10.73
IPC2_peptide 9.736
IPC2.peptide.svr19 8.546
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10637
0
10637
5133785
66
8847
482.6
53.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.304 ± 0.024
1.157 ± 0.008
5.896 ± 0.018
6.363 ± 0.024
3.508 ± 0.016
7.385 ± 0.022
2.338 ± 0.01
4.446 ± 0.015
4.601 ± 0.022
8.644 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.092 ± 0.008
3.49 ± 0.012
6.335 ± 0.027
3.855 ± 0.016
6.696 ± 0.022
7.877 ± 0.023
5.739 ± 0.017
6.151 ± 0.019
1.432 ± 0.007
2.691 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here