Amino acid dipepetide frequency for Microcystis aeruginosa (strain NIES-843)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.335AlaAla: 6.335 ± 0.097	0.705AlaCys: 0.705 ± 0.023	3.594AlaAsp: 3.594 ± 0.053	4.61AlaGlu: 4.61 ± 0.06	2.794AlaPhe: 2.794 ± 0.046	4.888AlaGly: 4.888 ± 0.078	1.116AlaHis: 1.116 ± 0.024	6.625AlaIle: 6.625 ± 0.079	4.335AlaLys: 4.335 ± 0.065	7.988AlaLeu: 7.988 ± 0.099	1.447AlaMet: 1.447 ± 0.029	3.209AlaAsn: 3.209 ± 0.061	2.487AlaPro: 2.487 ± 0.053	3.297AlaGln: 3.297 ± 0.047	3.528AlaArg: 3.528 ± 0.057	4.065AlaSer: 4.065 ± 0.054	4.158AlaThr: 4.158 ± 0.079	4.947AlaVal: 4.947 ± 0.066	1.022AlaTrp: 1.022 ± 0.031	2.314AlaTyr: 2.314 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.606CysAla: 0.606 ± 0.023	0.167CysCys: 0.167 ± 0.011	0.508CysAsp: 0.508 ± 0.019	0.521CysGlu: 0.521 ± 0.018	0.507CysPhe: 0.507 ± 0.017	0.892CysGly: 0.892 ± 0.027	0.323CysHis: 0.323 ± 0.02	0.492CysIle: 0.492 ± 0.02	0.328CysLys: 0.328 ± 0.017	1.421CysLeu: 1.421 ± 0.036	0.168CysMet: 0.168 ± 0.01	0.315CysAsn: 0.315 ± 0.013	0.634CysPro: 0.634 ± 0.02	0.87CysGln: 0.87 ± 0.025	0.676CysArg: 0.676 ± 0.021	0.704CysSer: 0.704 ± 0.023	0.404CysThr: 0.404 ± 0.015	0.611CysVal: 0.611 ± 0.023	0.129CysTrp: 0.129 ± 0.008	0.42CysTyr: 0.42 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	2.855AspAla: 2.855 ± 0.045	0.592AspCys: 0.592 ± 0.021	2.117AspAsp: 2.117 ± 0.047	2.778AspGlu: 2.778 ± 0.047	2.551AspPhe: 2.551 ± 0.04	3.063AspGly: 3.063 ± 0.053	0.923AspHis: 0.923 ± 0.027	3.328AspIle: 3.328 ± 0.053	2.684AspLys: 2.684 ± 0.044	5.884AspLeu: 5.884 ± 0.065	0.658AspMet: 0.658 ± 0.021	2.518AspAsn: 2.518 ± 0.045	2.184AspPro: 2.184 ± 0.04	1.786AspGln: 1.786 ± 0.031	3.395AspArg: 3.395 ± 0.055	3.071AspSer: 3.071 ± 0.049	2.254AspThr: 2.254 ± 0.058	2.479AspVal: 2.479 ± 0.043	0.993AspTrp: 0.993 ± 0.028	2.177AspTyr: 2.177 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	4.853GluAla: 4.853 ± 0.075	0.454GluCys: 0.454 ± 0.018	2.921GluAsp: 2.921 ± 0.046	4.706GluGlu: 4.706 ± 0.075	2.357GluPhe: 2.357 ± 0.045	3.396GluGly: 3.396 ± 0.055	0.886GluHis: 0.886 ± 0.026	5.862GluIle: 5.862 ± 0.076	5.045GluLys: 5.045 ± 0.066	6.722GluLeu: 6.722 ± 0.078	1.279GluMet: 1.279 ± 0.029	3.303GluAsn: 3.303 ± 0.048	2.208GluPro: 2.208 ± 0.045	3.361GluGln: 3.361 ± 0.049	3.819GluArg: 3.819 ± 0.058	3.718GluSer: 3.718 ± 0.054	3.84GluThr: 3.84 ± 0.059	3.925GluVal: 3.925 ± 0.054	0.751GluTrp: 0.751 ± 0.026	1.911GluTyr: 1.911 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	2.888PheAla: 2.888 ± 0.046	0.641PheCys: 0.641 ± 0.02	2.187PheAsp: 2.187 ± 0.039	2.192PheGlu: 2.192 ± 0.046	1.997PhePhe: 1.997 ± 0.041	2.686PheGly: 2.686 ± 0.044	0.76PheHis: 0.76 ± 0.023	2.503PheIle: 2.503 ± 0.042	1.77PheLys: 1.77 ± 0.037	4.824PheLeu: 4.824 ± 0.061	0.704PheMet: 0.704 ± 0.024	1.888PheAsn: 1.888 ± 0.037	2.113PhePro: 2.113 ± 0.042	1.993PheGln: 1.993 ± 0.04	2.035PheArg: 2.035 ± 0.038	3.255PheSer: 3.255 ± 0.054	2.191PheThr: 2.191 ± 0.039	2.311PheVal: 2.311 ± 0.047	0.776PheTrp: 0.776 ± 0.024	1.596PheTyr: 1.596 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.078GlyAla: 4.078 ± 0.065	0.846GlyCys: 0.846 ± 0.023	3.359GlyAsp: 3.359 ± 0.054	4.313GlyGlu: 4.313 ± 0.054	3.007GlyPhe: 3.007 ± 0.051	4.667GlyGly: 4.667 ± 0.078	1.164GlyHis: 1.164 ± 0.034	5.278GlyIle: 5.278 ± 0.071	4.32GlyLys: 4.32 ± 0.065	6.789GlyLeu: 6.789 ± 0.081	1.354GlyMet: 1.354 ± 0.033	2.968GlyAsn: 2.968 ± 0.065	1.172GlyPro: 1.172 ± 0.031	3.107GlyGln: 3.107 ± 0.047	3.243GlyArg: 3.243 ± 0.051	3.892GlySer: 3.892 ± 0.066	3.575GlyThr: 3.575 ± 0.087	4.379GlyVal: 4.379 ± 0.06	1.027GlyTrp: 1.027 ± 0.028	2.399GlyTyr: 2.399 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	0.921HisAla: 0.921 ± 0.029	0.303HisCys: 0.303 ± 0.015	0.749HisAsp: 0.749 ± 0.021	0.762HisGlu: 0.762 ± 0.023	0.882HisPhe: 0.882 ± 0.023	1.015HisGly: 1.015 ± 0.028	0.559HisHis: 0.559 ± 0.025	1.049HisIle: 1.049 ± 0.025	0.684HisLys: 0.684 ± 0.023	2.559HisLeu: 2.559 ± 0.049	0.135HisMet: 0.135 ± 0.009	0.654HisAsn: 0.654 ± 0.021	1.428HisPro: 1.428 ± 0.034	1.145HisGln: 1.145 ± 0.03	1.136HisArg: 1.136 ± 0.026	1.135HisSer: 1.135 ± 0.027	0.801HisThr: 0.801 ± 0.026	0.606HisVal: 0.606 ± 0.02	0.345HisTrp: 0.345 ± 0.017	0.662HisTyr: 0.662 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.719IleAla: 6.719 ± 0.066	0.819IleCys: 0.819 ± 0.029	4.14IleAsp: 4.14 ± 0.058	5.119IleGlu: 5.119 ± 0.067	2.961IlePhe: 2.961 ± 0.052	4.585IleGly: 4.585 ± 0.063	1.163IleHis: 1.163 ± 0.028	5.116IleIle: 5.116 ± 0.069	3.667IleLys: 3.667 ± 0.058	7.53IleLeu: 7.53 ± 0.071	0.966IleMet: 0.966 ± 0.025	3.456IleAsn: 3.456 ± 0.059	3.835IlePro: 3.835 ± 0.056	2.536IleGln: 2.536 ± 0.045	3.298IleArg: 3.298 ± 0.051	4.877IleSer: 4.877 ± 0.056	4.062IleThr: 4.062 ± 0.056	4.205IleVal: 4.205 ± 0.063	0.856IleTrp: 0.856 ± 0.026	2.34IleTyr: 2.34 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.154LysAla: 4.154 ± 0.061	0.479LysCys: 0.479 ± 0.021	2.427LysAsp: 2.427 ± 0.048	3.618LysGlu: 3.618 ± 0.066	1.823LysPhe: 1.823 ± 0.034	3.048LysGly: 3.048 ± 0.051	0.81LysHis: 0.81 ± 0.024	4.614LysIle: 4.614 ± 0.063	3.625LysLys: 3.625 ± 0.078	5.894LysLeu: 5.894 ± 0.073	1.112LysMet: 1.112 ± 0.025	3.017LysAsn: 3.017 ± 0.055	2.42LysPro: 2.42 ± 0.044	2.742LysGln: 2.742 ± 0.056	2.845LysArg: 2.845 ± 0.055	3.585LysSer: 3.585 ± 0.05	3.378LysThr: 3.378 ± 0.056	3.323LysVal: 3.323 ± 0.048	0.534LysTrp: 0.534 ± 0.019	1.674LysTyr: 1.674 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	9.612LeuAla: 9.612 ± 0.11	1.016LeuCys: 1.016 ± 0.03	5.587LeuAsp: 5.587 ± 0.068	8.331LeuGlu: 8.331 ± 0.096	3.958LeuPhe: 3.958 ± 0.058	8.039LeuGly: 8.039 ± 0.101	1.716LeuHis: 1.716 ± 0.04	7.726LeuIle: 7.726 ± 0.072	6.687LeuLys: 6.687 ± 0.087	11.173LeuLeu: 11.173 ± 0.114	1.968LeuMet: 1.968 ± 0.038	4.89LeuAsn: 4.89 ± 0.069	5.735LeuPro: 5.735 ± 0.081	5.044LeuGln: 5.044 ± 0.07	5.344LeuArg: 5.344 ± 0.07	7.947LeuSer: 7.947 ± 0.089	6.858LeuThr: 6.858 ± 0.083	6.751LeuVal: 6.751 ± 0.067	1.518LeuTrp: 1.518 ± 0.04	2.911LeuTyr: 2.911 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.698MetAla: 1.698 ± 0.031	0.118MetCys: 0.118 ± 0.008	0.779MetAsp: 0.779 ± 0.023	0.969MetGlu: 0.969 ± 0.024	0.524MetPhe: 0.524 ± 0.018	1.348MetGly: 1.348 ± 0.033	0.169MetHis: 0.169 ± 0.011	1.271MetIle: 1.271 ± 0.027	0.993MetLys: 0.993 ± 0.025	1.733MetLeu: 1.733 ± 0.04	0.348MetMet: 0.348 ± 0.018	0.805MetAsn: 0.805 ± 0.025	0.807MetPro: 0.807 ± 0.025	0.666MetGln: 0.666 ± 0.022	0.929MetArg: 0.929 ± 0.025	1.223MetSer: 1.223 ± 0.028	1.355MetThr: 1.355 ± 0.032	1.255MetVal: 1.255 ± 0.028	0.158MetTrp: 0.158 ± 0.01	0.329MetTyr: 0.329 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.718AsnAla: 2.718 ± 0.045	0.645AsnCys: 0.645 ± 0.023	1.78AsnAsp: 1.78 ± 0.05	1.866AsnGlu: 1.866 ± 0.039	2.284AsnPhe: 2.284 ± 0.042	2.419AsnGly: 2.419 ± 0.059	0.953AsnHis: 0.953 ± 0.029	2.79AsnIle: 2.79 ± 0.049	1.825AsnLys: 1.825 ± 0.037	6.184AsnLeu: 6.184 ± 0.08	0.688AsnMet: 0.688 ± 0.025	2.312AsnAsn: 2.312 ± 0.055	3.314AsnPro: 3.314 ± 0.054	2.981AsnGln: 2.981 ± 0.056	2.647AsnArg: 2.647 ± 0.05	3.09AsnSer: 3.09 ± 0.063	2.039AsnThr: 2.039 ± 0.043	1.944AsnVal: 1.944 ± 0.036	0.85AsnTrp: 0.85 ± 0.026	1.937AsnTyr: 1.937 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	2.914ProAla: 2.914 ± 0.046	0.424ProCys: 0.424 ± 0.017	2.973ProAsp: 2.973 ± 0.047	3.744ProGlu: 3.744 ± 0.061	1.778ProPhe: 1.778 ± 0.039	2.297ProGly: 2.297 ± 0.051	1.108ProHis: 1.108 ± 0.039	3.222ProIle: 3.222 ± 0.053	2.336ProLys: 2.336 ± 0.041	5.158ProLeu: 5.158 ± 0.066	0.807ProMet: 0.807 ± 0.027	2.201ProAsn: 2.201 ± 0.039	2.453ProPro: 2.453 ± 0.053	2.542ProGln: 2.542 ± 0.042	2.009ProArg: 2.009 ± 0.042	3.16ProSer: 3.16 ± 0.052	2.682ProThr: 2.682 ± 0.06	2.931ProVal: 2.931 ± 0.05	0.572ProTrp: 0.572 ± 0.02	1.412ProTyr: 1.412 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.026GlnAla: 4.026 ± 0.055	0.332GlnCys: 0.332 ± 0.015	2.01GlnAsp: 2.01 ± 0.037	4.157GlnGlu: 4.157 ± 0.062	1.82GlnPhe: 1.82 ± 0.038	3.432GlnGly: 3.432 ± 0.051	0.606GlnHis: 0.606 ± 0.023	3.581GlnIle: 3.581 ± 0.049	3.498GlnLys: 3.498 ± 0.069	5.707GlnLeu: 5.707 ± 0.072	0.923GlnMet: 0.923 ± 0.022	1.899GlnAsn: 1.899 ± 0.034	2.107GlnPro: 2.107 ± 0.042	3.042GlnGln: 3.042 ± 0.058	2.783GlnArg: 2.783 ± 0.05	2.849GlnSer: 2.849 ± 0.046	2.514GlnThr: 2.514 ± 0.048	3.303GlnVal: 3.303 ± 0.051	0.747GlnTrp: 0.747 ± 0.026	1.264GlnTyr: 1.264 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	3.037ArgAla: 3.037 ± 0.059	0.58ArgCys: 0.58 ± 0.021	2.451ArgAsp: 2.451 ± 0.044	4.009ArgGlu: 4.009 ± 0.061	2.475ArgPhe: 2.475 ± 0.038	3.466ArgGly: 3.466 ± 0.055	1.032ArgHis: 1.032 ± 0.027	3.441ArgIle: 3.441 ± 0.055	2.608ArgLys: 2.608 ± 0.05	6.334ArgLeu: 6.334 ± 0.071	0.905ArgMet: 0.905 ± 0.027	2.078ArgAsn: 2.078 ± 0.041	2.163ArgPro: 2.163 ± 0.045	3.653ArgGln: 3.653 ± 0.052	3.112ArgArg: 3.112 ± 0.065	3.034ArgSer: 3.034 ± 0.045	2.294ArgThr: 2.294 ± 0.039	3.304ArgVal: 3.304 ± 0.049	1.001ArgTrp: 1.001 ± 0.031	1.871ArgTyr: 1.871 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	4.043SerAla: 4.043 ± 0.06	0.799SerCys: 0.799 ± 0.025	3.175SerAsp: 3.175 ± 0.052	3.663SerGlu: 3.663 ± 0.054	2.622SerPhe: 2.622 ± 0.041	4.343SerGly: 4.343 ± 0.07	1.297SerHis: 1.297 ± 0.03	3.968SerIle: 3.968 ± 0.056	2.883SerLys: 2.883 ± 0.047	8.632SerLeu: 8.632 ± 0.092	1.216SerMet: 1.216 ± 0.025	2.615SerAsn: 2.615 ± 0.051	3.923SerPro: 3.923 ± 0.063	3.761SerGln: 3.761 ± 0.055	3.384SerArg: 3.384 ± 0.049	4.429SerSer: 4.429 ± 0.078	2.896SerThr: 2.896 ± 0.054	3.67SerVal: 3.67 ± 0.056	1.01SerTrp: 1.01 ± 0.028	2.06SerTyr: 2.06 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	4.623ThrAla: 4.623 ± 0.083	0.473ThrCys: 0.473 ± 0.021	2.594ThrAsp: 2.594 ± 0.046	3.361ThrGlu: 3.361 ± 0.052	2.082ThrPhe: 2.082 ± 0.04	4.263ThrGly: 4.263 ± 0.08	0.913ThrHis: 0.913 ± 0.025	4.156ThrIle: 4.156 ± 0.075	2.223ThrLys: 2.223 ± 0.037	6.158ThrLeu: 6.158 ± 0.088	0.82ThrMet: 0.82 ± 0.025	2.116ThrAsn: 2.116 ± 0.041	3.178ThrPro: 3.178 ± 0.057	2.148ThrGln: 2.148 ± 0.043	2.457ThrArg: 2.457 ± 0.042	3.212ThrSer: 3.212 ± 0.056	3.011ThrThr: 3.011 ± 0.063	3.909ThrVal: 3.909 ± 0.059	0.675ThrTrp: 0.675 ± 0.018	1.647ThrTyr: 1.647 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	4.769ValAla: 4.769 ± 0.062	0.661ValCys: 0.661 ± 0.022	2.811ValAsp: 2.811 ± 0.047	4.007ValGlu: 4.007 ± 0.058	2.673ValPhe: 2.673 ± 0.044	3.97ValGly: 3.97 ± 0.052	0.884ValHis: 0.884 ± 0.028	4.618ValIle: 4.618 ± 0.056	3.537ValLys: 3.537 ± 0.048	5.724ValLeu: 5.724 ± 0.066	1.301ValMet: 1.301 ± 0.032	2.972ValAsn: 2.972 ± 0.057	2.42ValPro: 2.42 ± 0.047	2.165ValGln: 2.165 ± 0.039	3.068ValArg: 3.068 ± 0.051	4.101ValSer: 4.101 ± 0.061	3.545ValThr: 3.545 ± 0.056	3.792ValVal: 3.792 ± 0.059	0.866ValTrp: 0.866 ± 0.026	1.877ValTyr: 1.877 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	0.766TrpAla: 0.766 ± 0.024	0.175TrpCys: 0.175 ± 0.01	0.623TrpAsp: 0.623 ± 0.025	0.86TrpGlu: 0.86 ± 0.026	0.643TrpPhe: 0.643 ± 0.021	1.092TrpGly: 1.092 ± 0.028	0.351TrpHis: 0.351 ± 0.015	0.845TrpIle: 0.845 ± 0.023	0.696TrpLys: 0.696 ± 0.024	2.171TrpLeu: 2.171 ± 0.043	0.282TrpMet: 0.282 ± 0.014	0.565TrpAsn: 0.565 ± 0.022	0.318TrpPro: 0.318 ± 0.016	1.327TrpGln: 1.327 ± 0.034	0.876TrpArg: 0.876 ± 0.021	0.899TrpSer: 0.899 ± 0.027	0.643TrpThr: 0.643 ± 0.021	0.756TrpVal: 0.756 ± 0.023	0.212TrpTrp: 0.212 ± 0.012	0.519TrpTyr: 0.519 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.85TyrAla: 1.85 ± 0.038	0.469TyrCys: 0.469 ± 0.018	1.503TyrAsp: 1.503 ± 0.034	1.729TyrGlu: 1.729 ± 0.038	1.572TyrPhe: 1.572 ± 0.033	2.031TyrGly: 2.031 ± 0.034	0.798TyrHis: 0.798 ± 0.027	1.766TyrIle: 1.766 ± 0.036	1.379TyrLys: 1.379 ± 0.029	4.226TyrLeu: 4.226 ± 0.062	0.396TyrMet: 0.396 ± 0.014	1.484TyrAsn: 1.484 ± 0.039	1.822TyrPro: 1.822 ± 0.037	2.434TyrGln: 2.434 ± 0.047	2.265TyrArg: 2.265 ± 0.036	2.14TyrSer: 2.14 ± 0.041	1.518TyrThr: 1.518 ± 0.032	1.36TyrVal: 1.36 ± 0.032	0.58TyrTrp: 0.58 ± 0.02	1.253TyrTyr: 1.253 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5981 proteins (1510946 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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