Paenibacillus sp. (strain JDR-2)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6D864|C6D864_PAESJ DNA repair protein RadC OS=Paenibacillus sp. (strain JDR-2) OX=324057 GN=Pjdr2_4423 PE=3 SV=1
MM1 pKa = 7.13YY2 pKa = 9.68CVCKK6 pKa = 10.08QHH8 pKa = 6.99VEE10 pKa = 3.77LAIDD14 pKa = 3.65KK15 pKa = 10.67FVDD18 pKa = 4.01EE19 pKa = 5.32YY20 pKa = 11.35EE21 pKa = 4.39DD22 pKa = 4.27APDD25 pKa = 3.86IVDD28 pKa = 3.85LKK30 pKa = 9.85EE31 pKa = 4.05TEE33 pKa = 4.13FADD36 pKa = 3.3WDD38 pKa = 3.97PPRR41 pKa = 11.84SCDD44 pKa = 3.89LCGEE48 pKa = 4.33AAAEE52 pKa = 4.02FLVVV56 pKa = 3.72

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6D8C4|C6D8C4_PAESJ Uncharacterized protein OS=Paenibacillus sp. (strain JDR-2) OX=324057 GN=Pjdr2_4483 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.74PNVSKK11 pKa = 10.78RR12 pKa = 11.84SKK14 pKa = 9.04VHH16 pKa = 5.83GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSSKK25 pKa = 10.47NGRR28 pKa = 11.84KK29 pKa = 9.01VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6210

0

6210

2103411

26

4640

338.7

37.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.625 ± 0.042

0.692 ± 0.009

5.214 ± 0.025

6.518 ± 0.038

4.135 ± 0.022

7.568 ± 0.036

1.987 ± 0.014

6.605 ± 0.033

5.304 ± 0.03

9.912 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.019

4.016 ± 0.027

4.033 ± 0.02

3.8 ± 0.019

4.825 ± 0.031

6.429 ± 0.028

5.579 ± 0.043

6.996 ± 0.026

1.372 ± 0.014

3.615 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski