Candidatus Aquiluna sp. IMCC13023

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Luna cluster; Luna-1 subcluster; Aquiluna; unclassified Aquiluna

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1364 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0R1S5|I0R1S5_9MICO Peptidyl-prolyl cis-trans isomerase OS=Candidatus Aquiluna sp. IMCC13023 OX=1081644 GN=IMCC13023_02900 PE=3 SV=1
MM1 pKa = 7.53IKK3 pKa = 10.45SKK5 pKa = 9.4TFSMLAIGATAALVLAGCAAAEE27 pKa = 4.41TEE29 pKa = 4.55TEE31 pKa = 4.3TEE33 pKa = 4.23TTAGTEE39 pKa = 3.81AATSIIGAYY48 pKa = 9.3DD49 pKa = 3.64LSGIDD54 pKa = 3.15ITVGSKK60 pKa = 10.89DD61 pKa = 3.47FDD63 pKa = 3.58EE64 pKa = 4.2QLILGEE70 pKa = 4.03MLVAAFEE77 pKa = 4.32EE78 pKa = 4.66AGATVDD84 pKa = 3.75NKK86 pKa = 10.51VNLGGTSVARR96 pKa = 11.84AALEE100 pKa = 4.23SGDD103 pKa = 2.93IDD105 pKa = 4.8IYY107 pKa = 10.64MEE109 pKa = 4.17YY110 pKa = 10.64NGTGWAVHH118 pKa = 6.77LGQEE122 pKa = 4.66DD123 pKa = 4.3PSFDD127 pKa = 4.18PDD129 pKa = 3.45VLTPEE134 pKa = 4.24VAALDD139 pKa = 3.81LSEE142 pKa = 5.51NGIVWVGRR150 pKa = 11.84SPFNNTYY157 pKa = 10.74GFASSPAVTEE167 pKa = 4.23ANGGAFTLTSMMEE180 pKa = 4.0YY181 pKa = 9.33TQANPDD187 pKa = 4.34AIVCMEE193 pKa = 4.35SEE195 pKa = 4.73FPSRR199 pKa = 11.84PDD201 pKa = 3.56GLILTEE207 pKa = 3.97NHH209 pKa = 6.49SGITLPDD216 pKa = 3.36SQQRR220 pKa = 11.84ILDD223 pKa = 3.3TGIIYY228 pKa = 9.73TEE230 pKa = 4.36TSANNCDD237 pKa = 3.71FGEE240 pKa = 4.72VYY242 pKa = 9.73ATDD245 pKa = 3.43GRR247 pKa = 11.84IPALGLTLVTDD258 pKa = 4.18PGVNILYY265 pKa = 9.94NVSGTIGQDD274 pKa = 3.21KK275 pKa = 10.8YY276 pKa = 11.78NEE278 pKa = 4.34APDD281 pKa = 3.99AFDD284 pKa = 5.78AIINAILAPLTNVRR298 pKa = 11.84MAEE301 pKa = 3.95LNGYY305 pKa = 9.12VSAEE309 pKa = 4.12GQDD312 pKa = 3.45SGVVARR318 pKa = 11.84EE319 pKa = 3.87YY320 pKa = 10.85LVSEE324 pKa = 4.29GLIDD328 pKa = 3.64

Molecular weight: 34.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0R302|I0R302_9MICO rRNA (Guanine-N2-)-methyltransferase OS=Candidatus Aquiluna sp. IMCC13023 OX=1081644 GN=IMCC13023_07170 PE=4 SV=1
MM1 pKa = 7.89PPRR4 pKa = 11.84LGTSLCSNSSRR15 pKa = 11.84FVLFDD20 pKa = 3.39LRR22 pKa = 11.84DD23 pKa = 4.18FPRR26 pKa = 11.84EE27 pKa = 3.88PPLPNAPAVPAPRR40 pKa = 11.84PPRR43 pKa = 11.84PAGPGLPAGGPNPGFCPGAPNAGAPPGRR71 pKa = 11.84GAPPPGRR78 pKa = 11.84GAPPAGLGAPGRR90 pKa = 11.84GAPGRR95 pKa = 11.84GADD98 pKa = 3.93PAGLGAPGRR107 pKa = 11.84DD108 pKa = 2.78RR109 pKa = 11.84PMPWFAANGLFPGRR123 pKa = 11.84TIGRR127 pKa = 11.84GIPMPWFEE135 pKa = 5.1EE136 pKa = 3.89NGLLPGRR143 pKa = 11.84GVPSIVAAADD153 pKa = 4.17FSSTTGSPASGAEE166 pKa = 3.99AGSGSTATGAAGSVMSEE183 pKa = 4.09GFGLSATAGASGAGLAFGFAGSFLAGASFFGKK215 pKa = 10.54APFSLAATGGVIVEE229 pKa = 4.71EE230 pKa = 4.62PPLTYY235 pKa = 10.51SPSSSNLAKK244 pKa = 10.41AVLVSIPSSLAMSYY258 pKa = 9.63TRR260 pKa = 11.84GSATFFLLFGADD272 pKa = 3.68PKK274 pKa = 10.78QGRR277 pKa = 11.84PP278 pKa = 3.3

Molecular weight: 27.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1364

0

1364

417606

37

1517

306.2

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.677 ± 0.078

0.537 ± 0.016

5.414 ± 0.054

6.347 ± 0.074

3.806 ± 0.046

8.128 ± 0.048

1.679 ± 0.03

6.065 ± 0.052

4.442 ± 0.053

10.58 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.029

3.166 ± 0.033

4.334 ± 0.039

3.54 ± 0.042

5.16 ± 0.046

6.997 ± 0.052

5.499 ± 0.053

8.027 ± 0.065

1.197 ± 0.027

2.19 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski